Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine®
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-3107bor-2019.vol33.0060 http://hdl.handle.net/11449/190586 |
Resumo: | This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine® (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (µm2) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student's t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p<0.05). In rats, hypertension was able to increase inflammatory infiltrate and decrease biomineralization of the tested materials. |
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spelling |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine®This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine® (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (µm2) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student's t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p<0.05). In rats, hypertension was able to increase inflammatory infiltrate and decrease biomineralization of the tested materials.Universidade Estadual Paulista - Unesp School of Dentistry Departament of EndodonticsUniversidade Federal de Alfenas - Unifal School of Dentistry Departament of SurgeryUniversidade Estadual Paulista - Unesp School of Dentistry Departament of EndodonticsUniversidade Estadual Paulista (Unesp)School of DentistryCosme-Silva, Leopoldo [UNESP]Dal Fabbro, Renan [UNESP]Gonçalves, Luanna de Oliveira [UNESP]Prado, Alana Sant'Ana do [UNESP]Plazza, Flávia Alfredo [UNESP]Viola, Naiana VianaCintra, Luciano Tavares Angelo [UNESP]Gomes Filho, João Eduardo [UNESP]2019-10-06T17:18:17Z2019-10-06T17:18:17Z2019-07-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee060application/pdfhttp://dx.doi.org/10.1590/1807-3107bor-2019.vol33.0060Brazilian oral research, v. 33, p. e060-.1807-3107http://hdl.handle.net/11449/19058610.1590/1807-3107bor-2019.vol33.0060S1806-832420190001002482-s2.0-85070880470S1806-83242019000100248.pdf92357430816673629235743081667362Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian oral researchinfo:eu-repo/semantics/openAccess2024-01-02T06:24:37Zoai:repositorio.unesp.br:11449/190586Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-02T06:24:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
spellingShingle |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® Cosme-Silva, Leopoldo [UNESP] |
title_short |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title_full |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title_fullStr |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title_full_unstemmed |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
title_sort |
Hypertension affects the biocompatibility and biomineralization of MTA, High-plasticity MTA, and Biodentine® |
author |
Cosme-Silva, Leopoldo [UNESP] |
author_facet |
Cosme-Silva, Leopoldo [UNESP] Dal Fabbro, Renan [UNESP] Gonçalves, Luanna de Oliveira [UNESP] Prado, Alana Sant'Ana do [UNESP] Plazza, Flávia Alfredo [UNESP] Viola, Naiana Viana Cintra, Luciano Tavares Angelo [UNESP] Gomes Filho, João Eduardo [UNESP] |
author_role |
author |
author2 |
Dal Fabbro, Renan [UNESP] Gonçalves, Luanna de Oliveira [UNESP] Prado, Alana Sant'Ana do [UNESP] Plazza, Flávia Alfredo [UNESP] Viola, Naiana Viana Cintra, Luciano Tavares Angelo [UNESP] Gomes Filho, João Eduardo [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) School of Dentistry |
dc.contributor.author.fl_str_mv |
Cosme-Silva, Leopoldo [UNESP] Dal Fabbro, Renan [UNESP] Gonçalves, Luanna de Oliveira [UNESP] Prado, Alana Sant'Ana do [UNESP] Plazza, Flávia Alfredo [UNESP] Viola, Naiana Viana Cintra, Luciano Tavares Angelo [UNESP] Gomes Filho, João Eduardo [UNESP] |
description |
This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine® (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (µm2) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student's t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p<0.05). In rats, hypertension was able to increase inflammatory infiltrate and decrease biomineralization of the tested materials. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:18:17Z 2019-10-06T17:18:17Z 2019-07-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-3107bor-2019.vol33.0060 Brazilian oral research, v. 33, p. e060-. 1807-3107 http://hdl.handle.net/11449/190586 10.1590/1807-3107bor-2019.vol33.0060 S1806-83242019000100248 2-s2.0-85070880470 S1806-83242019000100248.pdf 9235743081667362 9235743081667362 |
url |
http://dx.doi.org/10.1590/1807-3107bor-2019.vol33.0060 http://hdl.handle.net/11449/190586 |
identifier_str_mv |
Brazilian oral research, v. 33, p. e060-. 1807-3107 10.1590/1807-3107bor-2019.vol33.0060 S1806-83242019000100248 2-s2.0-85070880470 S1806-83242019000100248.pdf 9235743081667362 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian oral research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e060 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965494472605696 |