Prevalence and molecular characterization of Mycoplasma ovis in selected free-ranging Brazilian deer populations

• Autor(a) principal: Grazziotin, Ana Laura
• Data de Publicação: 2011
• Outros Autores: Duarte, José Maurício Barbanti [UNESP], Szabó, Matias Pablo Juan, Santos, Andrea Pires, Guimarães, Ana Marcia Sa, Mohamed, Ahmed, da Costa Vieira, Rafael Felipe, Filho, Ivan Roque de Barrosf, Biondo, Alexander Welker, Messick, Joanne Belle
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.7589/0090-3558-47.4.1005; http://hdl.handle.net/11449/226651
• topic: Blastocerus dichotomus; Deer; Hemoplasma; Hemotropic mycoplasma; Molecular characterization; Mycoplasma ovis; Ozotocerus bezoarticus; Prevalence

Mycoplasma ovis is a hemoplasma that may cause anemia and mortality in small ruminants. Our aim was to determine whether M. ovis infects populations of free-ranging deer in Brazil. Buffy coat samples from 64 Blastocerus dichotomus from Porto Primavera, 18 Ozotocerus bezoarticus from Pantanal, and 21 O. bezoarticus from Emas National Park were tested. Using a M. ovis PCR protocol to amplify extracted DNA, 46/64 (72%) of deer from Porto Primavera, 10/18 (56%) from Pantanal, and 4/ 21 (19%) from Emas National Park were positive, giving an overall positive rate of 58% for hemoplasma in these wild deer. Sequencing and phylogenetic analysis of the 16S rRNA gene revealed 3 genetically distinct hemoplasmas including M. ovis, 'Candidatus Mycoplasma erythrocervae', and a hemoplasma most closely related to M. ovis. Phylogenetic analysis of the 23S rRNA gene from selected sequences confirmed these relationships. © Wildlife Disease Association 2011.