Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4162-7965 http://hdl.handle.net/11449/178254 |
Resumo: | The aim of the present study was to characterize changes in acute phase protein levels according to the occurrence of rotavirus diarrhea in calves in the first month of life. Blood and fecal samples were taken before colostrum intake and at 1, 2, 7, 15, 21 and 30 days of age from 24 Holstein calves allotted in three experimental groups: calves that did not present diarrhea (group A), calves that presented diarrhea, but tested negative for rotavirus in feces (group B), and calves that presented diarrhea and tested positive for rotavirus in feces (group C) (experiment 1). When the animals presented episodes of diarrhea, blood and fecal samples were taken at 24-hour intervals until the end of clinical signs (experiment 2). Serum proteins were separated by SDS-PAGE technique and rotavirus in feces was detected by PAGE. Data of experiment 1 were analyzed by ANOVA and Tukey's test, considered significant at P<0.05. Data of experiment 2 were subjected to the HSD test. Total protein, globulins, and IgG concentrations were lower in group C than in groups A and B. Ceruloplasmin and transferrin levels were higher in group C than in groups A and B. Serum concentrations of haptoglobin and α1-acid glycoprotein did not differ significantly between groups throughout the experimental period. Calves presented diarrhea between 10.4 and 14.6 days of age in group B, and between 10.3 and 14.6 days of age in group C. In the moments of diarrhea manifestation, least square means of IgA, haptoglobin and a1-acid glycoprotein concentrations did not differ significantly between groups B and C, but ceruloplasmin and transferrin concentrations were higher in group C than in group B, as opposed to what occurred with IgG levels. These findings show that optimizing passive immunity transfer of immunoglobulins decrease the likelihood of calves developing diarrhea caused by rotavirus. In addition, ceruloplasmin presents characteristics of a biomarker of rotavirus infection in calves. |
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Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrheaBovine neonatal diarrheaBovine rotavirusCeruloplasminSerum proteinogramThe aim of the present study was to characterize changes in acute phase protein levels according to the occurrence of rotavirus diarrhea in calves in the first month of life. Blood and fecal samples were taken before colostrum intake and at 1, 2, 7, 15, 21 and 30 days of age from 24 Holstein calves allotted in three experimental groups: calves that did not present diarrhea (group A), calves that presented diarrhea, but tested negative for rotavirus in feces (group B), and calves that presented diarrhea and tested positive for rotavirus in feces (group C) (experiment 1). When the animals presented episodes of diarrhea, blood and fecal samples were taken at 24-hour intervals until the end of clinical signs (experiment 2). Serum proteins were separated by SDS-PAGE technique and rotavirus in feces was detected by PAGE. Data of experiment 1 were analyzed by ANOVA and Tukey's test, considered significant at P<0.05. Data of experiment 2 were subjected to the HSD test. Total protein, globulins, and IgG concentrations were lower in group C than in groups A and B. Ceruloplasmin and transferrin levels were higher in group C than in groups A and B. Serum concentrations of haptoglobin and α1-acid glycoprotein did not differ significantly between groups throughout the experimental period. Calves presented diarrhea between 10.4 and 14.6 days of age in group B, and between 10.3 and 14.6 days of age in group C. In the moments of diarrhea manifestation, least square means of IgA, haptoglobin and a1-acid glycoprotein concentrations did not differ significantly between groups B and C, but ceruloplasmin and transferrin concentrations were higher in group C than in group B, as opposed to what occurred with IgG levels. These findings show that optimizing passive immunity transfer of immunoglobulins decrease the likelihood of calves developing diarrhea caused by rotavirus. In addition, ceruloplasmin presents characteristics of a biomarker of rotavirus infection in calves.Universidade Estadual Paulista-UNESPUniversidade de São Paulo-USPEmbrapa Pecuária SudesteUniversidade Estadual Paulista-UNESPUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Rocha, T. G. [UNESP]Silva, F. D.F.Bortoletto, C. [UNESP]Silva, D. G. [UNESP]Buzinaro, M. G. [UNESP]Zafalon, L. F.Fagliari, J. J. [UNESP]2018-12-11T17:29:30Z2018-12-11T17:29:30Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article865-872application/pdfhttp://dx.doi.org/10.1590/1678-4162-7965Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 68, n. 4, p. 865-872, 2016.1678-41620102-0935http://hdl.handle.net/11449/17825410.1590/1678-4162-7965S0102-093520160004008652-s2.0-84984918748S0102-09352016000400865.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinaria e Zootecnia0,248info:eu-repo/semantics/openAccess2024-01-22T06:27:59Zoai:repositorio.unesp.br:11449/178254Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-22T06:27:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea |
title |
Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea |
spellingShingle |
Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea Rocha, T. G. [UNESP] Bovine neonatal diarrhea Bovine rotavirus Ceruloplasmin Serum proteinogram |
title_short |
Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea |
title_full |
Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea |
title_fullStr |
Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea |
title_full_unstemmed |
Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea |
title_sort |
Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea |
author |
Rocha, T. G. [UNESP] |
author_facet |
Rocha, T. G. [UNESP] Silva, F. D.F. Bortoletto, C. [UNESP] Silva, D. G. [UNESP] Buzinaro, M. G. [UNESP] Zafalon, L. F. Fagliari, J. J. [UNESP] |
author_role |
author |
author2 |
Silva, F. D.F. Bortoletto, C. [UNESP] Silva, D. G. [UNESP] Buzinaro, M. G. [UNESP] Zafalon, L. F. Fagliari, J. J. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Rocha, T. G. [UNESP] Silva, F. D.F. Bortoletto, C. [UNESP] Silva, D. G. [UNESP] Buzinaro, M. G. [UNESP] Zafalon, L. F. Fagliari, J. J. [UNESP] |
dc.subject.por.fl_str_mv |
Bovine neonatal diarrhea Bovine rotavirus Ceruloplasmin Serum proteinogram |
topic |
Bovine neonatal diarrhea Bovine rotavirus Ceruloplasmin Serum proteinogram |
description |
The aim of the present study was to characterize changes in acute phase protein levels according to the occurrence of rotavirus diarrhea in calves in the first month of life. Blood and fecal samples were taken before colostrum intake and at 1, 2, 7, 15, 21 and 30 days of age from 24 Holstein calves allotted in three experimental groups: calves that did not present diarrhea (group A), calves that presented diarrhea, but tested negative for rotavirus in feces (group B), and calves that presented diarrhea and tested positive for rotavirus in feces (group C) (experiment 1). When the animals presented episodes of diarrhea, blood and fecal samples were taken at 24-hour intervals until the end of clinical signs (experiment 2). Serum proteins were separated by SDS-PAGE technique and rotavirus in feces was detected by PAGE. Data of experiment 1 were analyzed by ANOVA and Tukey's test, considered significant at P<0.05. Data of experiment 2 were subjected to the HSD test. Total protein, globulins, and IgG concentrations were lower in group C than in groups A and B. Ceruloplasmin and transferrin levels were higher in group C than in groups A and B. Serum concentrations of haptoglobin and α1-acid glycoprotein did not differ significantly between groups throughout the experimental period. Calves presented diarrhea between 10.4 and 14.6 days of age in group B, and between 10.3 and 14.6 days of age in group C. In the moments of diarrhea manifestation, least square means of IgA, haptoglobin and a1-acid glycoprotein concentrations did not differ significantly between groups B and C, but ceruloplasmin and transferrin concentrations were higher in group C than in group B, as opposed to what occurred with IgG levels. These findings show that optimizing passive immunity transfer of immunoglobulins decrease the likelihood of calves developing diarrhea caused by rotavirus. In addition, ceruloplasmin presents characteristics of a biomarker of rotavirus infection in calves. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:29:30Z 2018-12-11T17:29:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4162-7965 Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 68, n. 4, p. 865-872, 2016. 1678-4162 0102-0935 http://hdl.handle.net/11449/178254 10.1590/1678-4162-7965 S0102-09352016000400865 2-s2.0-84984918748 S0102-09352016000400865.pdf |
url |
http://dx.doi.org/10.1590/1678-4162-7965 http://hdl.handle.net/11449/178254 |
identifier_str_mv |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 68, n. 4, p. 865-872, 2016. 1678-4162 0102-0935 10.1590/1678-4162-7965 S0102-09352016000400865 2-s2.0-84984918748 S0102-09352016000400865.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia 0,248 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
865-872 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790369609416704 |