Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/biom13030553 http://hdl.handle.net/11449/249801 |
Resumo: | Mosquito females of the genus Mansonia (Blanchard) can be a nuisance to humans and animals since they are voraciously hematophagous and feed on the blood of a variety of vertebrates. Despite their relevance, there is a lack of investigation into the blood-feeding patterns of the Mansonia species. Knowledge of the host preference is crucial in establishing the public health importance of a mosquito species and its potential to be involved in the transmission dynamics of pathogens. Species that are primarily anthropophilic can be more effective in spreading vector-borne pathogens to humans. In this study, we used an Illumina Nextera sequencing protocol and the QIIME2 workflow to assess the diversity of DNA sequences extracted in the ingested blood of mosquito species to evaluate the overall and local host choices for three species: Ma. titillans, Ma. Amazonensis, and Ma. humeralis, in rural areas alongside the Madeira River in the vicinities of the Santo Antonio Energia (SAE) reservoir in the municipality of Porto Velho, Rondônia, Western Brazil. By performing our analysis pipeline, we have found that host diversity per collection site showed a significant heterogeneity across the sample sites. In addition, in rural areas, Ma. amazonensis present a high affinity for B. taurus, Ma. humeralis shows an overall preference for C. familiaris and B. taurus, but also H. sapiens and E. caballus in urban areas, and Ma. titillans showed more opportunistic behavior in rural areas, feeding on wild animals and G. gallus, though with an overall preference for H. sapiens. |
id |
UNSP_686fb67e6997ec46b574377635257435 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/249801 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazilbiting activityblood sourceMansoniasppNGSMosquito females of the genus Mansonia (Blanchard) can be a nuisance to humans and animals since they are voraciously hematophagous and feed on the blood of a variety of vertebrates. Despite their relevance, there is a lack of investigation into the blood-feeding patterns of the Mansonia species. Knowledge of the host preference is crucial in establishing the public health importance of a mosquito species and its potential to be involved in the transmission dynamics of pathogens. Species that are primarily anthropophilic can be more effective in spreading vector-borne pathogens to humans. In this study, we used an Illumina Nextera sequencing protocol and the QIIME2 workflow to assess the diversity of DNA sequences extracted in the ingested blood of mosquito species to evaluate the overall and local host choices for three species: Ma. titillans, Ma. Amazonensis, and Ma. humeralis, in rural areas alongside the Madeira River in the vicinities of the Santo Antonio Energia (SAE) reservoir in the municipality of Porto Velho, Rondônia, Western Brazil. By performing our analysis pipeline, we have found that host diversity per collection site showed a significant heterogeneity across the sample sites. In addition, in rural areas, Ma. amazonensis present a high affinity for B. taurus, Ma. humeralis shows an overall preference for C. familiaris and B. taurus, but also H. sapiens and E. caballus in urban areas, and Ma. titillans showed more opportunistic behavior in rural areas, feeding on wild animals and G. gallus, though with an overall preference for H. sapiens.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Biotechnology Institute and Bioscience Institute Sao Paulo State UniversityDepartamento de Epidemiologia Faculdade de Saúde Pública Universidade de São PauloSanto Antônio EnergiaBiotechnology Institute and Bioscience Institute Sao Paulo State UniversityCNPq: 301877/2016-5Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Santo Antônio EnergiaAlonso, Diego Peres [UNESP]Amorim, Jandui Almeidade Oliveira, Tatiane Marques Porangabade Sá, Ivy Luizi RodriguesPossebon, Fábio Sossai [UNESP]de Carvalho, Dario PiresRibeiro, Kaio Augusto NabasRibolla, Paulo Eduardo Martins [UNESP]Sallum, Maria Anice Mureb2023-07-29T16:09:35Z2023-07-29T16:09:35Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/biom13030553Biomolecules, v. 13, n. 3, 2023.2218-273Xhttp://hdl.handle.net/11449/24980110.3390/biom130305532-s2.0-85151193321Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomoleculesinfo:eu-repo/semantics/openAccess2023-07-29T16:09:35Zoai:repositorio.unesp.br:11449/249801Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:09:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil |
title |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil |
spellingShingle |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil Alonso, Diego Peres [UNESP] biting activity blood source Mansoniaspp NGS |
title_short |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil |
title_full |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil |
title_fullStr |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil |
title_full_unstemmed |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil |
title_sort |
Host Feeding Patterns of Mansonia (Diptera, Culicidae) in Rural Settlements near Porto Velho, State of Rondonia, Brazil |
author |
Alonso, Diego Peres [UNESP] |
author_facet |
Alonso, Diego Peres [UNESP] Amorim, Jandui Almeida de Oliveira, Tatiane Marques Porangaba de Sá, Ivy Luizi Rodrigues Possebon, Fábio Sossai [UNESP] de Carvalho, Dario Pires Ribeiro, Kaio Augusto Nabas Ribolla, Paulo Eduardo Martins [UNESP] Sallum, Maria Anice Mureb |
author_role |
author |
author2 |
Amorim, Jandui Almeida de Oliveira, Tatiane Marques Porangaba de Sá, Ivy Luizi Rodrigues Possebon, Fábio Sossai [UNESP] de Carvalho, Dario Pires Ribeiro, Kaio Augusto Nabas Ribolla, Paulo Eduardo Martins [UNESP] Sallum, Maria Anice Mureb |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Santo Antônio Energia |
dc.contributor.author.fl_str_mv |
Alonso, Diego Peres [UNESP] Amorim, Jandui Almeida de Oliveira, Tatiane Marques Porangaba de Sá, Ivy Luizi Rodrigues Possebon, Fábio Sossai [UNESP] de Carvalho, Dario Pires Ribeiro, Kaio Augusto Nabas Ribolla, Paulo Eduardo Martins [UNESP] Sallum, Maria Anice Mureb |
dc.subject.por.fl_str_mv |
biting activity blood source Mansoniaspp NGS |
topic |
biting activity blood source Mansoniaspp NGS |
description |
Mosquito females of the genus Mansonia (Blanchard) can be a nuisance to humans and animals since they are voraciously hematophagous and feed on the blood of a variety of vertebrates. Despite their relevance, there is a lack of investigation into the blood-feeding patterns of the Mansonia species. Knowledge of the host preference is crucial in establishing the public health importance of a mosquito species and its potential to be involved in the transmission dynamics of pathogens. Species that are primarily anthropophilic can be more effective in spreading vector-borne pathogens to humans. In this study, we used an Illumina Nextera sequencing protocol and the QIIME2 workflow to assess the diversity of DNA sequences extracted in the ingested blood of mosquito species to evaluate the overall and local host choices for three species: Ma. titillans, Ma. Amazonensis, and Ma. humeralis, in rural areas alongside the Madeira River in the vicinities of the Santo Antonio Energia (SAE) reservoir in the municipality of Porto Velho, Rondônia, Western Brazil. By performing our analysis pipeline, we have found that host diversity per collection site showed a significant heterogeneity across the sample sites. In addition, in rural areas, Ma. amazonensis present a high affinity for B. taurus, Ma. humeralis shows an overall preference for C. familiaris and B. taurus, but also H. sapiens and E. caballus in urban areas, and Ma. titillans showed more opportunistic behavior in rural areas, feeding on wild animals and G. gallus, though with an overall preference for H. sapiens. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:09:35Z 2023-07-29T16:09:35Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/biom13030553 Biomolecules, v. 13, n. 3, 2023. 2218-273X http://hdl.handle.net/11449/249801 10.3390/biom13030553 2-s2.0-85151193321 |
url |
http://dx.doi.org/10.3390/biom13030553 http://hdl.handle.net/11449/249801 |
identifier_str_mv |
Biomolecules, v. 13, n. 3, 2023. 2218-273X 10.3390/biom13030553 2-s2.0-85151193321 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomolecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964552763277312 |