Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate

Detalhes bibliográficos
Autor(a) principal: Botello Suárez, Wilmar Alirio [UNESP]
Data de Publicação: 2018
Outros Autores: da Silva Vantini, Juliana [UNESP], Duda, Rose Maria, Giachetto, Poliana Fernanda, Cintra, Leandro Carrijo, Tiraboschi Ferro, Maria Inês [UNESP], de Oliveira, Roberto Alves [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.biortech.2018.06.091
http://hdl.handle.net/11449/176657
Resumo: The effect of the organic loading rate (OLR) on the performance and microbial composition of a two-stage UASB system treating coffee processing wastewater was assessed. The system was operated with OLR up to 18.2 g COD (L d)−1 and effluent recirculation. Methane production and effluent characteristics were monitored. The microbial composition was examined through next-generation sequencing and qPCR from the anaerobic sludge of the first reactor (R1) operated at low and high OLR. The system showed operational stability, obtaining a maximum methane production of 2.2 L CH4 (L d)−1, with a removal efficiency of COD and phenolic compounds of 84 and 73%, respectively. The performance of R1 at high OLR in steady conditions was associated with an appropriate proportion of nutrients (particularly Fe) and a marked increase of the syntrophic bacteria Syntrophus and Candidatus Cloacimonas, and acetoclastic and hydrogenotrophic methanogens, mainly Methanosaeta, Methanoculleus, Methanobacterium and Methanomassiliicoccus.
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spelling Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rateAnaerobic digestionBiogasMetagenomics of anaerobic sludgeMethanogenic archaeaPollutant removalThe effect of the organic loading rate (OLR) on the performance and microbial composition of a two-stage UASB system treating coffee processing wastewater was assessed. The system was operated with OLR up to 18.2 g COD (L d)−1 and effluent recirculation. Methane production and effluent characteristics were monitored. The microbial composition was examined through next-generation sequencing and qPCR from the anaerobic sludge of the first reactor (R1) operated at low and high OLR. The system showed operational stability, obtaining a maximum methane production of 2.2 L CH4 (L d)−1, with a removal efficiency of COD and phenolic compounds of 84 and 73%, respectively. The performance of R1 at high OLR in steady conditions was associated with an appropriate proportion of nutrients (particularly Fe) and a marked increase of the syntrophic bacteria Syntrophus and Candidatus Cloacimonas, and acetoclastic and hydrogenotrophic methanogens, mainly Methanosaeta, Methanoculleus, Methanobacterium and Methanomassiliicoccus.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Post-Graduate Program in Agricultural and Livestock Microbiology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalLaboratory of Environmental Sanitation Department of Rural Engineering São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalLaboratory of Biochemistry and Molecular Biology Department of Technology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalFaculty of Technology, JaboticabalEmbrapa Informática Agropecuária, CampinasPost-Graduate Program in Agricultural and Livestock Microbiology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalLaboratory of Environmental Sanitation Department of Rural Engineering São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalLaboratory of Biochemistry and Molecular Biology Department of Technology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, JaboticabalUniversidade Estadual Paulista (Unesp)Faculty of TechnologyEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Botello Suárez, Wilmar Alirio [UNESP]da Silva Vantini, Juliana [UNESP]Duda, Rose MariaGiachetto, Poliana FernandaCintra, Leandro CarrijoTiraboschi Ferro, Maria Inês [UNESP]de Oliveira, Roberto Alves [UNESP]2018-12-11T17:21:57Z2018-12-11T17:21:57Z2018-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article158-168application/pdfhttp://dx.doi.org/10.1016/j.biortech.2018.06.091Bioresource Technology, v. 268, p. 158-168.1873-29760960-8524http://hdl.handle.net/11449/17665710.1016/j.biortech.2018.06.0912-s2.0-850507995982-s2.0-85050799598.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioresource Technology2,029info:eu-repo/semantics/openAccess2024-01-12T06:23:47Zoai:repositorio.unesp.br:11449/176657Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-12T06:23:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
title Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
spellingShingle Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
Botello Suárez, Wilmar Alirio [UNESP]
Anaerobic digestion
Biogas
Metagenomics of anaerobic sludge
Methanogenic archaea
Pollutant removal
title_short Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
title_full Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
title_fullStr Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
title_full_unstemmed Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
title_sort Predominance of syntrophic bacteria, Methanosaeta and Methanoculleus in a two-stage up-flow anaerobic sludge blanket reactor treating coffee processing wastewater at high organic loading rate
author Botello Suárez, Wilmar Alirio [UNESP]
author_facet Botello Suárez, Wilmar Alirio [UNESP]
da Silva Vantini, Juliana [UNESP]
Duda, Rose Maria
Giachetto, Poliana Fernanda
Cintra, Leandro Carrijo
Tiraboschi Ferro, Maria Inês [UNESP]
de Oliveira, Roberto Alves [UNESP]
author_role author
author2 da Silva Vantini, Juliana [UNESP]
Duda, Rose Maria
Giachetto, Poliana Fernanda
Cintra, Leandro Carrijo
Tiraboschi Ferro, Maria Inês [UNESP]
de Oliveira, Roberto Alves [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Faculty of Technology
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Botello Suárez, Wilmar Alirio [UNESP]
da Silva Vantini, Juliana [UNESP]
Duda, Rose Maria
Giachetto, Poliana Fernanda
Cintra, Leandro Carrijo
Tiraboschi Ferro, Maria Inês [UNESP]
de Oliveira, Roberto Alves [UNESP]
dc.subject.por.fl_str_mv Anaerobic digestion
Biogas
Metagenomics of anaerobic sludge
Methanogenic archaea
Pollutant removal
topic Anaerobic digestion
Biogas
Metagenomics of anaerobic sludge
Methanogenic archaea
Pollutant removal
description The effect of the organic loading rate (OLR) on the performance and microbial composition of a two-stage UASB system treating coffee processing wastewater was assessed. The system was operated with OLR up to 18.2 g COD (L d)−1 and effluent recirculation. Methane production and effluent characteristics were monitored. The microbial composition was examined through next-generation sequencing and qPCR from the anaerobic sludge of the first reactor (R1) operated at low and high OLR. The system showed operational stability, obtaining a maximum methane production of 2.2 L CH4 (L d)−1, with a removal efficiency of COD and phenolic compounds of 84 and 73%, respectively. The performance of R1 at high OLR in steady conditions was associated with an appropriate proportion of nutrients (particularly Fe) and a marked increase of the syntrophic bacteria Syntrophus and Candidatus Cloacimonas, and acetoclastic and hydrogenotrophic methanogens, mainly Methanosaeta, Methanoculleus, Methanobacterium and Methanomassiliicoccus.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:21:57Z
2018-12-11T17:21:57Z
2018-11-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.biortech.2018.06.091
Bioresource Technology, v. 268, p. 158-168.
1873-2976
0960-8524
http://hdl.handle.net/11449/176657
10.1016/j.biortech.2018.06.091
2-s2.0-85050799598
2-s2.0-85050799598.pdf
url http://dx.doi.org/10.1016/j.biortech.2018.06.091
http://hdl.handle.net/11449/176657
identifier_str_mv Bioresource Technology, v. 268, p. 158-168.
1873-2976
0960-8524
10.1016/j.biortech.2018.06.091
2-s2.0-85050799598
2-s2.0-85050799598.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioresource Technology
2,029
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 158-168
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965593400508416

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