Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2014/412437 http://hdl.handle.net/11449/131012 |
Resumo: | Uncaria tomentosa is a medicinal plant used in folk medicine by Amazon tribes. In this study the constituents of aqueous extract of U. tomentosa bark were quantified by chromatographic technique and its lethal concentration 50 (48 h) in Hyphessobrycon eques was determined. The chromatography showed high levels of oxindole alkaloids, quinovic acid glycosides, and low molecular weight polyphenols. The CL50 48 h was 1816 mg/L. Fish showed behavior changes at concentrations above 2000 mg/L, accompanied by a significant decrease of dissolved oxygen. At the highest concentration 100% mortality was observed attributed to oxygen reduction by the amount of oxindole alkaloids, polyphenols accumulation of the extract in the gills, and the interaction of these compounds with dopamine. In conclusion, the aqueous extract of U. tomentosa did not alter the chemical components and it was shown that U. tomentosa has low toxicity to H. eques; therefore, it can be used safely in this species. |
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Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon equesUncaria tomentosa is a medicinal plant used in folk medicine by Amazon tribes. In this study the constituents of aqueous extract of U. tomentosa bark were quantified by chromatographic technique and its lethal concentration 50 (48 h) in Hyphessobrycon eques was determined. The chromatography showed high levels of oxindole alkaloids, quinovic acid glycosides, and low molecular weight polyphenols. The CL50 48 h was 1816 mg/L. Fish showed behavior changes at concentrations above 2000 mg/L, accompanied by a significant decrease of dissolved oxygen. At the highest concentration 100% mortality was observed attributed to oxygen reduction by the amount of oxindole alkaloids, polyphenols accumulation of the extract in the gills, and the interaction of these compounds with dopamine. In conclusion, the aqueous extract of U. tomentosa did not alter the chemical components and it was shown that U. tomentosa has low toxicity to H. eques; therefore, it can be used safely in this species.Aquaculture Center of UNESP, Jaboticabal, SP, Brazil.Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), 14884-900 Jaboticabal, SP, Brazil.Weed Science Environmental Research Studies Centre of UNESP, University Estadual Paulista, Jaboticabal, SP, Brazil.Faculty of Pharmacy, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.Aquaculture Center of UNESP, Jaboticabal, SP, Brazil ; Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), 14884-900 Jaboticabal, SP, Brazil.Aquaculture Center of UNESP, Jaboticabal, SP, Brazil.Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), 14884-900 Jaboticabal, SP, Brazil.Weed Science Environmental Research Studies Centre of UNESP, University Estadual Paulista, Jaboticabal, SP, Brazil.Aquaculture Center of UNESP, Jaboticabal, SP, Brazil ; Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences, São Paulo State University (UNESP), 14884-900 Jaboticabal, SP, Brazil.Hindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Universidade Federal do Rio Grande do Sul (UFRGS)Yunis Aguinaga, Jefferson [UNESP]Claudiano, Gustavo S. [UNESP]Marcusso, Paulo F. [UNESP]Ikefuti, Cynthia [UNESP]Ortega, George G.Eto, Silas F. [UNESP]da Cruz, Claudinei [UNESP]Moraes, Juliet R. E. [UNESP]Moraes, Flávio R. [UNESP]Fernandes, João B. K. [UNESP]2015-12-07T15:30:49Z2015-12-07T15:30:49Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-5application/pdfhttp://dx.doi.org/10.1155/2014/412437Journal Of Toxicology, v. 2014, p. 1-5, 2014.1687-8191http://hdl.handle.net/11449/13101210.1155/2014/412437PMC3964835.pdf24734041PMC3964835PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Toxicology0,515info:eu-repo/semantics/openAccess2024-04-09T15:10:26Zoai:repositorio.unesp.br:11449/131012Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:10:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques |
title |
Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques |
spellingShingle |
Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques Yunis Aguinaga, Jefferson [UNESP] |
title_short |
Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques |
title_full |
Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques |
title_fullStr |
Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques |
title_full_unstemmed |
Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques |
title_sort |
Acute toxicity and determination of the active constituents of aqueous extract of uncaria tomentosa bark in hyphessobrycon eques |
author |
Yunis Aguinaga, Jefferson [UNESP] |
author_facet |
Yunis Aguinaga, Jefferson [UNESP] Claudiano, Gustavo S. [UNESP] Marcusso, Paulo F. [UNESP] Ikefuti, Cynthia [UNESP] Ortega, George G. Eto, Silas F. [UNESP] da Cruz, Claudinei [UNESP] Moraes, Juliet R. E. [UNESP] Moraes, Flávio R. [UNESP] Fernandes, João B. K. [UNESP] |
author_role |
author |
author2 |
Claudiano, Gustavo S. [UNESP] Marcusso, Paulo F. [UNESP] Ikefuti, Cynthia [UNESP] Ortega, George G. Eto, Silas F. [UNESP] da Cruz, Claudinei [UNESP] Moraes, Juliet R. E. [UNESP] Moraes, Flávio R. [UNESP] Fernandes, João B. K. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do Rio Grande do Sul (UFRGS) |
dc.contributor.author.fl_str_mv |
Yunis Aguinaga, Jefferson [UNESP] Claudiano, Gustavo S. [UNESP] Marcusso, Paulo F. [UNESP] Ikefuti, Cynthia [UNESP] Ortega, George G. Eto, Silas F. [UNESP] da Cruz, Claudinei [UNESP] Moraes, Juliet R. E. [UNESP] Moraes, Flávio R. [UNESP] Fernandes, João B. K. [UNESP] |
description |
Uncaria tomentosa is a medicinal plant used in folk medicine by Amazon tribes. In this study the constituents of aqueous extract of U. tomentosa bark were quantified by chromatographic technique and its lethal concentration 50 (48 h) in Hyphessobrycon eques was determined. The chromatography showed high levels of oxindole alkaloids, quinovic acid glycosides, and low molecular weight polyphenols. The CL50 48 h was 1816 mg/L. Fish showed behavior changes at concentrations above 2000 mg/L, accompanied by a significant decrease of dissolved oxygen. At the highest concentration 100% mortality was observed attributed to oxygen reduction by the amount of oxindole alkaloids, polyphenols accumulation of the extract in the gills, and the interaction of these compounds with dopamine. In conclusion, the aqueous extract of U. tomentosa did not alter the chemical components and it was shown that U. tomentosa has low toxicity to H. eques; therefore, it can be used safely in this species. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-12-07T15:30:49Z 2015-12-07T15:30:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2014/412437 Journal Of Toxicology, v. 2014, p. 1-5, 2014. 1687-8191 http://hdl.handle.net/11449/131012 10.1155/2014/412437 PMC3964835.pdf 24734041 PMC3964835 |
url |
http://dx.doi.org/10.1155/2014/412437 http://hdl.handle.net/11449/131012 |
identifier_str_mv |
Journal Of Toxicology, v. 2014, p. 1-5, 2014. 1687-8191 10.1155/2014/412437 PMC3964835.pdf 24734041 PMC3964835 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Toxicology 0,515 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-5 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789309580869632 |