Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00436-019-06337-3 http://hdl.handle.net/11449/189128 |
Resumo: | Babesia bovis is the etiological agent of bovine babesiosis, a disease transmitted by Rhipicephalus microplus, which affects cattle herds in tropical and subtropical regions of the world, causing significant economic losses due to decreasing meat and milk yield. This study used molecular techniques to determine the occurrence and genetic diversity of B. bovis, based on the genes encoding the spherical body protein (sbp-2) and the merozoite surface antigens (MSAs) genes, in a herd of 400 Nellore (Bos indicus) sampled from beef cattle farms in the Pantanal region, state of Mato Grosso do Sul, Midwestern Brazil. The results of the nested PCR assays based on the sbp-2 gene indicated that 18 (4.5%) calves were positive for B. bovis; out of them, while 77.7% (14/18) were positive for the B. bovis msa-2b fragment, 66.6% (12/18) were positive for the msa-2c fragment. The phylogenetic analysis based on the maximum likelihood method using 14 sequences from msa-2b clones and 13 sequences from msa-2c clones indicated that the sequences detected in this study are clearly distributed in different cladograms. These findings corroborated the diversity analysis of the same sequences, which revealed the presence of 14 and 11 haplotypes of the msa-2b and msa-2c genes, respectively. Furthermore, the entropy analyses of amino acid sequences revealed 78 and 44 high entropy peaks with values ranging from 0.25 to 1.53 and from 0.27 to 1.09 for MSA-2B and MSA-2C, respectively. Therefore, the results indicate a low molecular occurrence of B. bovis in beef cattle sampled in the Brazilian Pantanal. Despite this, a high degree of genetic diversity was found in the analyzed B. bovis population, with possibly different haplotypes coexisting in the same animal and/or in the same studied herd. |
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Genetic diversity of Babesia bovis in beef cattle in a large wetland in BrazilBabesia bovisBovine babesiosisGenetic diversityMSAPantanalBabesia bovis is the etiological agent of bovine babesiosis, a disease transmitted by Rhipicephalus microplus, which affects cattle herds in tropical and subtropical regions of the world, causing significant economic losses due to decreasing meat and milk yield. This study used molecular techniques to determine the occurrence and genetic diversity of B. bovis, based on the genes encoding the spherical body protein (sbp-2) and the merozoite surface antigens (MSAs) genes, in a herd of 400 Nellore (Bos indicus) sampled from beef cattle farms in the Pantanal region, state of Mato Grosso do Sul, Midwestern Brazil. The results of the nested PCR assays based on the sbp-2 gene indicated that 18 (4.5%) calves were positive for B. bovis; out of them, while 77.7% (14/18) were positive for the B. bovis msa-2b fragment, 66.6% (12/18) were positive for the msa-2c fragment. The phylogenetic analysis based on the maximum likelihood method using 14 sequences from msa-2b clones and 13 sequences from msa-2c clones indicated that the sequences detected in this study are clearly distributed in different cladograms. These findings corroborated the diversity analysis of the same sequences, which revealed the presence of 14 and 11 haplotypes of the msa-2b and msa-2c genes, respectively. Furthermore, the entropy analyses of amino acid sequences revealed 78 and 44 high entropy peaks with values ranging from 0.25 to 1.53 and from 0.27 to 1.09 for MSA-2B and MSA-2C, respectively. Therefore, the results indicate a low molecular occurrence of B. bovis in beef cattle sampled in the Brazilian Pantanal. Despite this, a high degree of genetic diversity was found in the analyzed B. bovis population, with possibly different haplotypes coexisting in the same animal and/or in the same studied herd.Agricultural and Livestock Microbiology Graduation Program School of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)Laboratory of Immunoparasitology Department of Veterinary Pathology Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona RuralUniversidade Católica Dom Bosco (UCDB)Agricultural and Livestock Microbiology Graduation Program School of Agricultural and Veterinarian Sciences São Paulo State University (UNESP)Laboratory of Immunoparasitology Department of Veterinary Pathology Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, Zona RuralUniversidade Estadual Paulista (Unesp)Universidade Católica Dom Bosco (UCDB)Mendes, Natalia Serra [UNESP]de Souza Ramos, Inalda Angélica [UNESP]Herrera, Heitor MiragliaCampos, João Bosco Vilelade Almeida Alves, João Victorde Macedo, Gabriel CarvalhoMachado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2019-10-06T16:30:41Z2019-10-06T16:30:41Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2027-2040http://dx.doi.org/10.1007/s00436-019-06337-3Parasitology Research, v. 118, n. 7, p. 2027-2040, 2019.1432-19550932-0113http://hdl.handle.net/11449/18912810.1007/s00436-019-06337-32-s2.0-850657357259139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Researchinfo:eu-repo/semantics/openAccess2021-10-22T21:10:09Zoai:repositorio.unesp.br:11449/189128Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:10:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil |
title |
Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil |
spellingShingle |
Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil Mendes, Natalia Serra [UNESP] Babesia bovis Bovine babesiosis Genetic diversity MSA Pantanal |
title_short |
Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil |
title_full |
Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil |
title_fullStr |
Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil |
title_full_unstemmed |
Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil |
title_sort |
Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil |
author |
Mendes, Natalia Serra [UNESP] |
author_facet |
Mendes, Natalia Serra [UNESP] de Souza Ramos, Inalda Angélica [UNESP] Herrera, Heitor Miraglia Campos, João Bosco Vilela de Almeida Alves, João Victor de Macedo, Gabriel Carvalho Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author_role |
author |
author2 |
de Souza Ramos, Inalda Angélica [UNESP] Herrera, Heitor Miraglia Campos, João Bosco Vilela de Almeida Alves, João Victor de Macedo, Gabriel Carvalho Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Católica Dom Bosco (UCDB) |
dc.contributor.author.fl_str_mv |
Mendes, Natalia Serra [UNESP] de Souza Ramos, Inalda Angélica [UNESP] Herrera, Heitor Miraglia Campos, João Bosco Vilela de Almeida Alves, João Victor de Macedo, Gabriel Carvalho Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
dc.subject.por.fl_str_mv |
Babesia bovis Bovine babesiosis Genetic diversity MSA Pantanal |
topic |
Babesia bovis Bovine babesiosis Genetic diversity MSA Pantanal |
description |
Babesia bovis is the etiological agent of bovine babesiosis, a disease transmitted by Rhipicephalus microplus, which affects cattle herds in tropical and subtropical regions of the world, causing significant economic losses due to decreasing meat and milk yield. This study used molecular techniques to determine the occurrence and genetic diversity of B. bovis, based on the genes encoding the spherical body protein (sbp-2) and the merozoite surface antigens (MSAs) genes, in a herd of 400 Nellore (Bos indicus) sampled from beef cattle farms in the Pantanal region, state of Mato Grosso do Sul, Midwestern Brazil. The results of the nested PCR assays based on the sbp-2 gene indicated that 18 (4.5%) calves were positive for B. bovis; out of them, while 77.7% (14/18) were positive for the B. bovis msa-2b fragment, 66.6% (12/18) were positive for the msa-2c fragment. The phylogenetic analysis based on the maximum likelihood method using 14 sequences from msa-2b clones and 13 sequences from msa-2c clones indicated that the sequences detected in this study are clearly distributed in different cladograms. These findings corroborated the diversity analysis of the same sequences, which revealed the presence of 14 and 11 haplotypes of the msa-2b and msa-2c genes, respectively. Furthermore, the entropy analyses of amino acid sequences revealed 78 and 44 high entropy peaks with values ranging from 0.25 to 1.53 and from 0.27 to 1.09 for MSA-2B and MSA-2C, respectively. Therefore, the results indicate a low molecular occurrence of B. bovis in beef cattle sampled in the Brazilian Pantanal. Despite this, a high degree of genetic diversity was found in the analyzed B. bovis population, with possibly different haplotypes coexisting in the same animal and/or in the same studied herd. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:30:41Z 2019-10-06T16:30:41Z 2019-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00436-019-06337-3 Parasitology Research, v. 118, n. 7, p. 2027-2040, 2019. 1432-1955 0932-0113 http://hdl.handle.net/11449/189128 10.1007/s00436-019-06337-3 2-s2.0-85065735725 9139899895580513 |
url |
http://dx.doi.org/10.1007/s00436-019-06337-3 http://hdl.handle.net/11449/189128 |
identifier_str_mv |
Parasitology Research, v. 118, n. 7, p. 2027-2040, 2019. 1432-1955 0932-0113 10.1007/s00436-019-06337-3 2-s2.0-85065735725 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2027-2040 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790286888304640 |