Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes)
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascibiolsci.v33i4.7045 http://hdl.handle.net/11449/72678 |
Resumo: | The present study investigates the δ 13C and δ 15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentatus. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze - control group (-15oC), conservation in alcohol 70% and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference was found in isotopic values of carbon and nitrogen, between the control and the samples in alcohol and formaldehyde, except for δ 13C from the H. edentatus samples in formaldehyde. The carbon isotopic values of samples in alcohol were mostly enriched compared to control, whereas the samples in formaldehyde presented depleted values in relation to the control. The nitrogen isotopic values for both samples preserved in alcohol and formaldehyde were enriched when compared to the values of frozen samples, independently of used preservatives. Therefore, the isotopic correction should be accomplished according to the isotope and preservative employed for species of freshwater fish. |
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Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes)Effect of alcohol and formaldehyde on the δ 13C and δ 15N isotopic composition of Plagioscion squamosissimus and hypophthalmus edentatus (Pisces, Osteichthyes)FishesHypophthalmus edentatusPlagioscion squamosissimusPreservativeStable isotopealcoholcarboncarbon 13delta carbon 13delta nitrogen 15formaldehydenitrogennitrogen 15unclassified druganimal tissuecontrolled studyfishfreezingnonhumanpreservationOsteichthyesPiscesThe present study investigates the δ 13C and δ 15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentatus. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze - control group (-15oC), conservation in alcohol 70% and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference was found in isotopic values of carbon and nitrogen, between the control and the samples in alcohol and formaldehyde, except for δ 13C from the H. edentatus samples in formaldehyde. The carbon isotopic values of samples in alcohol were mostly enriched compared to control, whereas the samples in formaldehyde presented depleted values in relation to the control. The nitrogen isotopic values for both samples preserved in alcohol and formaldehyde were enriched when compared to the values of frozen samples, independently of used preservatives. Therefore, the isotopic correction should be accomplished according to the isotope and preservative employed for species of freshwater fish.Universidade Estadual de Maringá, Av. Colombo, 5790, 87020-900, Maringá, ParanáUniversidade Estadual Paulista 'Júlio de Mesquita Filho', São PauloUniversidade Estadual Paulista 'Júlio de Mesquita Filho', São PauloUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Manetta, Gislaine IachstelBenedito, EvanildeDucatti, Carlos [UNESP]2014-05-27T11:26:00Z2014-05-27T11:26:00Z2011-09-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article393-397application/pdfhttp://dx.doi.org/10.4025/actascibiolsci.v33i4.7045Acta Scientiarum - Biological Sciences, v. 33, n. 4, p. 393-397, 2011.1679-92831807-863Xhttp://hdl.handle.net/11449/7267810.4025/actascibiolsci.v33i4.70452-s2.0-800525870362-s2.0-80052587036.pdf1030251743943217Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporActa Scientiarum: Biological Sciences0,208info:eu-repo/semantics/openAccess2023-11-12T06:13:46Zoai:repositorio.unesp.br:11449/72678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-12T06:13:46Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes) Effect of alcohol and formaldehyde on the δ 13C and δ 15N isotopic composition of Plagioscion squamosissimus and hypophthalmus edentatus (Pisces, Osteichthyes) |
title |
Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes) |
spellingShingle |
Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes) Manetta, Gislaine Iachstel Fishes Hypophthalmus edentatus Plagioscion squamosissimus Preservative Stable isotope alcohol carbon carbon 13 delta carbon 13 delta nitrogen 15 formaldehyde nitrogen nitrogen 15 unclassified drug animal tissue controlled study fish freezing nonhuman preservation Osteichthyes Pisces |
title_short |
Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes) |
title_full |
Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes) |
title_fullStr |
Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes) |
title_full_unstemmed |
Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes) |
title_sort |
Efeito do álcool e formol sobre a composição isotópica de δ 13C e de δ 15N em Plagioscion squamosissimus e Hypophthalmus edentatus (Pisces, Osteichthyes) |
author |
Manetta, Gislaine Iachstel |
author_facet |
Manetta, Gislaine Iachstel Benedito, Evanilde Ducatti, Carlos [UNESP] |
author_role |
author |
author2 |
Benedito, Evanilde Ducatti, Carlos [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Manetta, Gislaine Iachstel Benedito, Evanilde Ducatti, Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Fishes Hypophthalmus edentatus Plagioscion squamosissimus Preservative Stable isotope alcohol carbon carbon 13 delta carbon 13 delta nitrogen 15 formaldehyde nitrogen nitrogen 15 unclassified drug animal tissue controlled study fish freezing nonhuman preservation Osteichthyes Pisces |
topic |
Fishes Hypophthalmus edentatus Plagioscion squamosissimus Preservative Stable isotope alcohol carbon carbon 13 delta carbon 13 delta nitrogen 15 formaldehyde nitrogen nitrogen 15 unclassified drug animal tissue controlled study fish freezing nonhuman preservation Osteichthyes Pisces |
description |
The present study investigates the δ 13C and δ 15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentatus. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze - control group (-15oC), conservation in alcohol 70% and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference was found in isotopic values of carbon and nitrogen, between the control and the samples in alcohol and formaldehyde, except for δ 13C from the H. edentatus samples in formaldehyde. The carbon isotopic values of samples in alcohol were mostly enriched compared to control, whereas the samples in formaldehyde presented depleted values in relation to the control. The nitrogen isotopic values for both samples preserved in alcohol and formaldehyde were enriched when compared to the values of frozen samples, independently of used preservatives. Therefore, the isotopic correction should be accomplished according to the isotope and preservative employed for species of freshwater fish. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-14 2014-05-27T11:26:00Z 2014-05-27T11:26:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascibiolsci.v33i4.7045 Acta Scientiarum - Biological Sciences, v. 33, n. 4, p. 393-397, 2011. 1679-9283 1807-863X http://hdl.handle.net/11449/72678 10.4025/actascibiolsci.v33i4.7045 2-s2.0-80052587036 2-s2.0-80052587036.pdf 1030251743943217 |
url |
http://dx.doi.org/10.4025/actascibiolsci.v33i4.7045 http://hdl.handle.net/11449/72678 |
identifier_str_mv |
Acta Scientiarum - Biological Sciences, v. 33, n. 4, p. 393-397, 2011. 1679-9283 1807-863X 10.4025/actascibiolsci.v33i4.7045 2-s2.0-80052587036 2-s2.0-80052587036.pdf 1030251743943217 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Acta Scientiarum: Biological Sciences 0,208 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
393-397 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964911373123584 |