Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anaerobe.2022.102588 http://hdl.handle.net/11449/240154 |
Resumo: | Objectives: Periodontitis is a pathology resulting from complex interaction of microorganisms in the dental biofilm with the host's immune system. Increased use of antibiotics associated with their inappropriate use has increased resistance levels in anaerobic bacteria. Therefore, identifying new antimicrobial compounds, such as chalcones, is urgent. This study evaluates the antibacterial activity and the antibiofilm activity of 15 chalcones against the periodontopathogenic bacteria Prevotella nigrescens (ATCC 33563), P. oralis (ATCC 33269), Peptostreptococcus anaerobius (ATCC 27337), Actinomyces viscosus (ATCC 43146), Porphyromonas asaccharolytica (ATCC 25260), and Fusobacterium nucleatum (ATCC 25586). Methods: The compounds were evaluated by minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) tests. Results: Compounds 1–6 showed good antibacterial and antibiofilm activities against most of the evaluated bacteria: MIC was lower than or equal to 6.25 μg/mL, biofilm biomass was reduced by 95%, and the compounds at concentrations between 0.78 and 100 μg/mL totally inhibited cell viability. Among the tested chalcones, 3 stood out: it was effective against all the bacteria, as revealed by the MIC and MBIC results. Conclusions: Our results have consolidated a base for the development of new studies on the effects of the tested chalcones as agents to combat and to prevent periodontitis. |
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Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteriaAnaerobic bacteriaAntibacterial activityAntibiofilm activityChalconePeriodontitisObjectives: Periodontitis is a pathology resulting from complex interaction of microorganisms in the dental biofilm with the host's immune system. Increased use of antibiotics associated with their inappropriate use has increased resistance levels in anaerobic bacteria. Therefore, identifying new antimicrobial compounds, such as chalcones, is urgent. This study evaluates the antibacterial activity and the antibiofilm activity of 15 chalcones against the periodontopathogenic bacteria Prevotella nigrescens (ATCC 33563), P. oralis (ATCC 33269), Peptostreptococcus anaerobius (ATCC 27337), Actinomyces viscosus (ATCC 43146), Porphyromonas asaccharolytica (ATCC 25260), and Fusobacterium nucleatum (ATCC 25586). Methods: The compounds were evaluated by minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) tests. Results: Compounds 1–6 showed good antibacterial and antibiofilm activities against most of the evaluated bacteria: MIC was lower than or equal to 6.25 μg/mL, biofilm biomass was reduced by 95%, and the compounds at concentrations between 0.78 and 100 μg/mL totally inhibited cell viability. Among the tested chalcones, 3 stood out: it was effective against all the bacteria, as revealed by the MIC and MBIC results. Conclusions: Our results have consolidated a base for the development of new studies on the effects of the tested chalcones as agents to combat and to prevent periodontitis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Antimicrobial Testing (LEA) Institute of Biomedical Sciences Federal University of Uberlândia, MGUniversity of Franca, SPLaboratory of Antibiotics and Chemotherapeutics (LAQ) Department of Chemistry and Environmental Sciences São Paulo State University, SPLaboratory of Antibiotics and Chemotherapeutics (LAQ) Department of Chemistry and Environmental Sciences São Paulo State University, SPFAPESP: 2014/18330–0FAPESP: 2018/15083–2Universidade Federal de Uberlândia (UFU)University of FrancaUniversidade Estadual Paulista (UNESP)Satokata, Alessandra Akemi CurySouza, Jonathan HenriqueSilva, Luana Luiza OliveiraSantiago, Mariana BrentiniRamos, Salvador BoccalettiAssis, Leticia Ribeiro de [UNESP]Theodoro, Reinaldo dos Santos [UNESP]Oliveira, Lígia Rodrigues e [UNESP]Regasini, Luis Octavio [UNESP]Martins, Carlos Henrique Gomes2023-03-01T20:03:51Z2023-03-01T20:03:51Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.anaerobe.2022.102588Anaerobe, v. 76.1095-82741075-9964http://hdl.handle.net/11449/24015410.1016/j.anaerobe.2022.1025882-s2.0-85130940359Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnaerobeinfo:eu-repo/semantics/openAccess2023-03-01T20:03:52Zoai:repositorio.unesp.br:11449/240154Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:03:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria |
title |
Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria |
spellingShingle |
Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria Satokata, Alessandra Akemi Cury Anaerobic bacteria Antibacterial activity Antibiofilm activity Chalcone Periodontitis |
title_short |
Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria |
title_full |
Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria |
title_fullStr |
Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria |
title_full_unstemmed |
Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria |
title_sort |
Chalcones with potential antibacterial and antibiofilm activities against periodontopathogenic bacteria |
author |
Satokata, Alessandra Akemi Cury |
author_facet |
Satokata, Alessandra Akemi Cury Souza, Jonathan Henrique Silva, Luana Luiza Oliveira Santiago, Mariana Brentini Ramos, Salvador Boccaletti Assis, Leticia Ribeiro de [UNESP] Theodoro, Reinaldo dos Santos [UNESP] Oliveira, Lígia Rodrigues e [UNESP] Regasini, Luis Octavio [UNESP] Martins, Carlos Henrique Gomes |
author_role |
author |
author2 |
Souza, Jonathan Henrique Silva, Luana Luiza Oliveira Santiago, Mariana Brentini Ramos, Salvador Boccaletti Assis, Leticia Ribeiro de [UNESP] Theodoro, Reinaldo dos Santos [UNESP] Oliveira, Lígia Rodrigues e [UNESP] Regasini, Luis Octavio [UNESP] Martins, Carlos Henrique Gomes |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) University of Franca Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Satokata, Alessandra Akemi Cury Souza, Jonathan Henrique Silva, Luana Luiza Oliveira Santiago, Mariana Brentini Ramos, Salvador Boccaletti Assis, Leticia Ribeiro de [UNESP] Theodoro, Reinaldo dos Santos [UNESP] Oliveira, Lígia Rodrigues e [UNESP] Regasini, Luis Octavio [UNESP] Martins, Carlos Henrique Gomes |
dc.subject.por.fl_str_mv |
Anaerobic bacteria Antibacterial activity Antibiofilm activity Chalcone Periodontitis |
topic |
Anaerobic bacteria Antibacterial activity Antibiofilm activity Chalcone Periodontitis |
description |
Objectives: Periodontitis is a pathology resulting from complex interaction of microorganisms in the dental biofilm with the host's immune system. Increased use of antibiotics associated with their inappropriate use has increased resistance levels in anaerobic bacteria. Therefore, identifying new antimicrobial compounds, such as chalcones, is urgent. This study evaluates the antibacterial activity and the antibiofilm activity of 15 chalcones against the periodontopathogenic bacteria Prevotella nigrescens (ATCC 33563), P. oralis (ATCC 33269), Peptostreptococcus anaerobius (ATCC 27337), Actinomyces viscosus (ATCC 43146), Porphyromonas asaccharolytica (ATCC 25260), and Fusobacterium nucleatum (ATCC 25586). Methods: The compounds were evaluated by minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) tests. Results: Compounds 1–6 showed good antibacterial and antibiofilm activities against most of the evaluated bacteria: MIC was lower than or equal to 6.25 μg/mL, biofilm biomass was reduced by 95%, and the compounds at concentrations between 0.78 and 100 μg/mL totally inhibited cell viability. Among the tested chalcones, 3 stood out: it was effective against all the bacteria, as revealed by the MIC and MBIC results. Conclusions: Our results have consolidated a base for the development of new studies on the effects of the tested chalcones as agents to combat and to prevent periodontitis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 2023-03-01T20:03:51Z 2023-03-01T20:03:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anaerobe.2022.102588 Anaerobe, v. 76. 1095-8274 1075-9964 http://hdl.handle.net/11449/240154 10.1016/j.anaerobe.2022.102588 2-s2.0-85130940359 |
url |
http://dx.doi.org/10.1016/j.anaerobe.2022.102588 http://hdl.handle.net/11449/240154 |
identifier_str_mv |
Anaerobe, v. 76. 1095-8274 1075-9964 10.1016/j.anaerobe.2022.102588 2-s2.0-85130940359 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anaerobe |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965270926688256 |