Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pathogens11101067 http://hdl.handle.net/11449/247808 |
Resumo: | The aim of this study was to evaluate the effect of the Cymbopogon citratus essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined by broth microdilution assay. Microcosms (polymicrobial) biofilms were produced on glass coverslips, using inoculum from human saliva in McBain culture medium (0.5% sucrose exposure for 6 h/day) for 3 days in 24-well plates. The biofilms were treated twice a day and their composition was evaluated by microorganism quantification. The acidogenicity was evaluated by measuring the pH of the spent culture medium in contact with the biofilm. Overall, the association of C. citratus and chlorhexidine reduced total bacterial counts and aciduric bacteria (maximum reduction of 3.55 log UFC/mL) in microcosm biofilms. This group also presented the lowest acidogenicity even when exposed to sucrose-containing medium. C. citratus essential oil increases the effect of digluconate chlorhexidine on microcosm biofilms. Based on these findings, this study can contribute to the development of new formulations that might allow for the use of mouthwashes for a shorter period, which may reduce undesirable effects and increase patient compliance to the treatment. |
id |
UNSP_6d6a2003619c42f64d08ab0f00334eb9 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/247808 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilmsbiofilmbiological productsdental cariesessential oilsphytotherapyThe aim of this study was to evaluate the effect of the Cymbopogon citratus essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined by broth microdilution assay. Microcosms (polymicrobial) biofilms were produced on glass coverslips, using inoculum from human saliva in McBain culture medium (0.5% sucrose exposure for 6 h/day) for 3 days in 24-well plates. The biofilms were treated twice a day and their composition was evaluated by microorganism quantification. The acidogenicity was evaluated by measuring the pH of the spent culture medium in contact with the biofilm. Overall, the association of C. citratus and chlorhexidine reduced total bacterial counts and aciduric bacteria (maximum reduction of 3.55 log UFC/mL) in microcosm biofilms. This group also presented the lowest acidogenicity even when exposed to sucrose-containing medium. C. citratus essential oil increases the effect of digluconate chlorhexidine on microcosm biofilms. Based on these findings, this study can contribute to the development of new formulations that might allow for the use of mouthwashes for a shorter period, which may reduce undesirable effects and increase patient compliance to the treatment.Department of Morphology Genetics Orthodontics and Pediatric Dentistry Araraquara Dental School São Paulo State University—UNESP, R Humaitá, 1680Department of Environment Engineering Institute of Science and Technology São José dos Campos São Paulo State University—UNESP, Av Eng Francisco José Longo, 777Department of Plant Biology Institute of Biology University of Campinas—UNICAMP, R Carlos Gomes, 241Department of Morphology Genetics Orthodontics and Pediatric Dentistry Araraquara Dental School São Paulo State University—UNESP, R Humaitá, 1680Department of Environment Engineering Institute of Science and Technology São José dos Campos São Paulo State University—UNESP, Av Eng Francisco José Longo, 777Universidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Mouta, Luís Felipe Garcia Leal [UNESP]Marques, Raquel Souza [UNESP]Koga-Ito, Cristiane Yumi [UNESP]Salvador, Marcos JoséGiro, Elisa Maria Aparecida [UNESP]Brighenti, Fernanda Lourenção [UNESP]2023-07-29T13:26:22Z2023-07-29T13:26:22Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/pathogens11101067Pathogens, v. 11, n. 10, 2022.2076-0817http://hdl.handle.net/11449/24780810.3390/pathogens111010672-s2.0-85140833838Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2023-07-29T13:26:22Zoai:repositorio.unesp.br:11449/247808Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:26:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms |
title |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms |
spellingShingle |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms Mouta, Luís Felipe Garcia Leal [UNESP] biofilm biological products dental caries essential oils phytotherapy |
title_short |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms |
title_full |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms |
title_fullStr |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms |
title_full_unstemmed |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms |
title_sort |
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms |
author |
Mouta, Luís Felipe Garcia Leal [UNESP] |
author_facet |
Mouta, Luís Felipe Garcia Leal [UNESP] Marques, Raquel Souza [UNESP] Koga-Ito, Cristiane Yumi [UNESP] Salvador, Marcos José Giro, Elisa Maria Aparecida [UNESP] Brighenti, Fernanda Lourenção [UNESP] |
author_role |
author |
author2 |
Marques, Raquel Souza [UNESP] Koga-Ito, Cristiane Yumi [UNESP] Salvador, Marcos José Giro, Elisa Maria Aparecida [UNESP] Brighenti, Fernanda Lourenção [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Mouta, Luís Felipe Garcia Leal [UNESP] Marques, Raquel Souza [UNESP] Koga-Ito, Cristiane Yumi [UNESP] Salvador, Marcos José Giro, Elisa Maria Aparecida [UNESP] Brighenti, Fernanda Lourenção [UNESP] |
dc.subject.por.fl_str_mv |
biofilm biological products dental caries essential oils phytotherapy |
topic |
biofilm biological products dental caries essential oils phytotherapy |
description |
The aim of this study was to evaluate the effect of the Cymbopogon citratus essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined by broth microdilution assay. Microcosms (polymicrobial) biofilms were produced on glass coverslips, using inoculum from human saliva in McBain culture medium (0.5% sucrose exposure for 6 h/day) for 3 days in 24-well plates. The biofilms were treated twice a day and their composition was evaluated by microorganism quantification. The acidogenicity was evaluated by measuring the pH of the spent culture medium in contact with the biofilm. Overall, the association of C. citratus and chlorhexidine reduced total bacterial counts and aciduric bacteria (maximum reduction of 3.55 log UFC/mL) in microcosm biofilms. This group also presented the lowest acidogenicity even when exposed to sucrose-containing medium. C. citratus essential oil increases the effect of digluconate chlorhexidine on microcosm biofilms. Based on these findings, this study can contribute to the development of new formulations that might allow for the use of mouthwashes for a shorter period, which may reduce undesirable effects and increase patient compliance to the treatment. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-01 2023-07-29T13:26:22Z 2023-07-29T13:26:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pathogens11101067 Pathogens, v. 11, n. 10, 2022. 2076-0817 http://hdl.handle.net/11449/247808 10.3390/pathogens11101067 2-s2.0-85140833838 |
url |
http://dx.doi.org/10.3390/pathogens11101067 http://hdl.handle.net/11449/247808 |
identifier_str_mv |
Pathogens, v. 11, n. 10, 2022. 2076-0817 10.3390/pathogens11101067 2-s2.0-85140833838 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965238959800320 |