Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2020.101817 http://hdl.handle.net/11449/200610 |
Resumo: | Background: An appropriate photosensitizer (PS) for photodynamic inactivation should have a pronounced antimicrobial efficacy but low dark toxicity. The aim of this study is to investigate the concentration-dependent antimicrobial efficacies of methylene blue (MB) and chlorin e6 (Ce6), against Streptococcus mutans biofilms and to compare the efficacies of these two PSs. Methods: The 48-h S. mutans UA159 biofilms, grown on glass coverslips, were subjected to MB or Ce6 at 25, 50, 100 and 200 μM with or without irradiation by 660 nM LED light (L). Control groups (-PS-L and -PS + L) were also included. Viability of the biofilm was analyzed by CFU/biofilm and biofilm lactic acid production was quantified by an enzymatic assay. Results: With irradiation, MB under 25 μM resulted in 2-log reduction in biofilm viability and 30-fold reduction in biofilm lactic acid production. However, this biofilm killing efficacy did not change with increasing MB concentration. The biofilm killing efficacy of Ce6 increased with increasing Ce6 concentrations and resulted in 5-log reduction in biofilm viability. The lactic acid inhibitory effect of Ce6 was significantly lower than MB at 25 μM (p<0.01) but higher than MB at 200 μM (p=0.05), although the difference at 200 μM did not reach statistical significance. No dark toxicity could be observed for MB whereas low dark toxicity could be seen for Ce6 when the concentration is above 50 μM. Conclusion: Ce6 under 200 μM showed to be a more powerful PS for photodynamic inactivation than MB. Both Ce6- and MB-based photodynamic inactivation are useful methods for biofilm control in caries prevention. |
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Repositório Institucional da UNESP |
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Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilmBiofilmChlorin e6Lactic acid productionMethylene bluePhotodynamic inactivationStreptococcus mutansBackground: An appropriate photosensitizer (PS) for photodynamic inactivation should have a pronounced antimicrobial efficacy but low dark toxicity. The aim of this study is to investigate the concentration-dependent antimicrobial efficacies of methylene blue (MB) and chlorin e6 (Ce6), against Streptococcus mutans biofilms and to compare the efficacies of these two PSs. Methods: The 48-h S. mutans UA159 biofilms, grown on glass coverslips, were subjected to MB or Ce6 at 25, 50, 100 and 200 μM with or without irradiation by 660 nM LED light (L). Control groups (-PS-L and -PS + L) were also included. Viability of the biofilm was analyzed by CFU/biofilm and biofilm lactic acid production was quantified by an enzymatic assay. Results: With irradiation, MB under 25 μM resulted in 2-log reduction in biofilm viability and 30-fold reduction in biofilm lactic acid production. However, this biofilm killing efficacy did not change with increasing MB concentration. The biofilm killing efficacy of Ce6 increased with increasing Ce6 concentrations and resulted in 5-log reduction in biofilm viability. The lactic acid inhibitory effect of Ce6 was significantly lower than MB at 25 μM (p<0.01) but higher than MB at 200 μM (p=0.05), although the difference at 200 μM did not reach statistical significance. No dark toxicity could be observed for MB whereas low dark toxicity could be seen for Ce6 when the concentration is above 50 μM. Conclusion: Ce6 under 200 μM showed to be a more powerful PS for photodynamic inactivation than MB. Both Ce6- and MB-based photodynamic inactivation are useful methods for biofilm control in caries prevention.State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan UniversityDepartment of Preventive Dentistry Academic Centre for Dentistry Amsterdam University of Amsterdam and Vrije Universiteit AmsterdamKey Lab of Oral Diseases of Gansu Province Northwest Minzu UniversityFederal University of São Carlos – UFSCar Department of ChemistryUniversity of São Paulo - USP Physics Institute of São Carlos - IFSC Optical Group Campus São CarlosUniversity of São Paulo State – UNESP Araraquara School of Dentistry Department of Restorative Dentistry, Campus AraraquaraUniversity of São Paulo State – UNESP Araraquara School of Dentistry Department of Restorative Dentistry, Campus AraraquaraSichuan UniversityUniversity of Amsterdam and Vrije Universiteit AmsterdamNorthwest Minzu UniversityUniversidade Federal de São Carlos (UFSCar)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Nie, MinDeng, Dong MeiWu, Yafeide Oliveira, Kleber ThiagoBagnato, Vanderlei SalvadorCrielaard, WimRastelli, Alessandra Nara de Souza [UNESP]2020-12-12T02:11:13Z2020-12-12T02:11:13Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.pdpdt.2020.101817Photodiagnosis and Photodynamic Therapy, v. 31.1873-15971572-1000http://hdl.handle.net/11449/20061010.1016/j.pdpdt.2020.1018172-s2.0-85086568778Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2021-10-23T14:48:17Zoai:repositorio.unesp.br:11449/200610Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:48:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm |
title |
Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm |
spellingShingle |
Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm Nie, Min Biofilm Chlorin e6 Lactic acid production Methylene blue Photodynamic inactivation Streptococcus mutans |
title_short |
Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm |
title_full |
Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm |
title_fullStr |
Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm |
title_full_unstemmed |
Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm |
title_sort |
Photodynamic inactivation mediated by methylene blue or chlorin e6 against Streptococcus mutans biofilm |
author |
Nie, Min |
author_facet |
Nie, Min Deng, Dong Mei Wu, Yafei de Oliveira, Kleber Thiago Bagnato, Vanderlei Salvador Crielaard, Wim Rastelli, Alessandra Nara de Souza [UNESP] |
author_role |
author |
author2 |
Deng, Dong Mei Wu, Yafei de Oliveira, Kleber Thiago Bagnato, Vanderlei Salvador Crielaard, Wim Rastelli, Alessandra Nara de Souza [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sichuan University University of Amsterdam and Vrije Universiteit Amsterdam Northwest Minzu University Universidade Federal de São Carlos (UFSCar) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nie, Min Deng, Dong Mei Wu, Yafei de Oliveira, Kleber Thiago Bagnato, Vanderlei Salvador Crielaard, Wim Rastelli, Alessandra Nara de Souza [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Chlorin e6 Lactic acid production Methylene blue Photodynamic inactivation Streptococcus mutans |
topic |
Biofilm Chlorin e6 Lactic acid production Methylene blue Photodynamic inactivation Streptococcus mutans |
description |
Background: An appropriate photosensitizer (PS) for photodynamic inactivation should have a pronounced antimicrobial efficacy but low dark toxicity. The aim of this study is to investigate the concentration-dependent antimicrobial efficacies of methylene blue (MB) and chlorin e6 (Ce6), against Streptococcus mutans biofilms and to compare the efficacies of these two PSs. Methods: The 48-h S. mutans UA159 biofilms, grown on glass coverslips, were subjected to MB or Ce6 at 25, 50, 100 and 200 μM with or without irradiation by 660 nM LED light (L). Control groups (-PS-L and -PS + L) were also included. Viability of the biofilm was analyzed by CFU/biofilm and biofilm lactic acid production was quantified by an enzymatic assay. Results: With irradiation, MB under 25 μM resulted in 2-log reduction in biofilm viability and 30-fold reduction in biofilm lactic acid production. However, this biofilm killing efficacy did not change with increasing MB concentration. The biofilm killing efficacy of Ce6 increased with increasing Ce6 concentrations and resulted in 5-log reduction in biofilm viability. The lactic acid inhibitory effect of Ce6 was significantly lower than MB at 25 μM (p<0.01) but higher than MB at 200 μM (p=0.05), although the difference at 200 μM did not reach statistical significance. No dark toxicity could be observed for MB whereas low dark toxicity could be seen for Ce6 when the concentration is above 50 μM. Conclusion: Ce6 under 200 μM showed to be a more powerful PS for photodynamic inactivation than MB. Both Ce6- and MB-based photodynamic inactivation are useful methods for biofilm control in caries prevention. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:11:13Z 2020-12-12T02:11:13Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2020.101817 Photodiagnosis and Photodynamic Therapy, v. 31. 1873-1597 1572-1000 http://hdl.handle.net/11449/200610 10.1016/j.pdpdt.2020.101817 2-s2.0-85086568778 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2020.101817 http://hdl.handle.net/11449/200610 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 31. 1873-1597 1572-1000 10.1016/j.pdpdt.2020.101817 2-s2.0-85086568778 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964847554691072 |