IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/jre.12940 http://hdl.handle.net/11449/229641 |
Resumo: | Background and objective: Previous studies have demonstrated an association between the IL10 promoter rs6667202 (C > A) single-nucleotide polymorphism (SNP) and grade C, stage 3 or 4 periodontitis (Perio4C) in the Brazilian population, where the altered A allele was detected more frequently in these patients. However, no functional analysis of this variation has yet been performed. Thus, the objective of this preliminary study was to evaluate the functionality of rs6667202 in gingival fibroblasts (GFs) of individuals with Perio4C and with periodontal health (PH) stimulated with Aggregatibacter actinomycetencomitans protein extract (AaPE). Methods: Patients with PH and Perio4C were segregated according to their genotype (AA, AC, or CC), and a biopsy was performed to establish the culture of the GFs. After GFs exposure to AaPE at 5 µg/ml for 1.5 h, RNA was extracted to analyze IL10 expression by qPCR. Aliquots of the cell's supernatant were subjected to immunoenzymatic analysis (MAGpix) to detect interleukin-10 (IL-10). Results: In PH, the genotypes AA and AC are related to less expression of IL10 (p = 0.027 and p < 0.0001) and less production of IL-10 (p = 0.002 and p = 0.001), when compared to CC. In Perio4C, there was no statistical difference between the genotypes (p > 0.05), although a lower IL-10 expression and release compared with PH CC was seen (p = 0.033 and p < 0.001). Conclusion: The rs6667202 SNP is functional in PH, as it decreases the expression and production of IL-10. In Perio4C, other factors may be masking its action by altering the IL-10's response. |
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Repositório Institucional da UNESP |
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spelling |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot studyBackground and objective: Previous studies have demonstrated an association between the IL10 promoter rs6667202 (C > A) single-nucleotide polymorphism (SNP) and grade C, stage 3 or 4 periodontitis (Perio4C) in the Brazilian population, where the altered A allele was detected more frequently in these patients. However, no functional analysis of this variation has yet been performed. Thus, the objective of this preliminary study was to evaluate the functionality of rs6667202 in gingival fibroblasts (GFs) of individuals with Perio4C and with periodontal health (PH) stimulated with Aggregatibacter actinomycetencomitans protein extract (AaPE). Methods: Patients with PH and Perio4C were segregated according to their genotype (AA, AC, or CC), and a biopsy was performed to establish the culture of the GFs. After GFs exposure to AaPE at 5 µg/ml for 1.5 h, RNA was extracted to analyze IL10 expression by qPCR. Aliquots of the cell's supernatant were subjected to immunoenzymatic analysis (MAGpix) to detect interleukin-10 (IL-10). Results: In PH, the genotypes AA and AC are related to less expression of IL10 (p = 0.027 and p < 0.0001) and less production of IL-10 (p = 0.002 and p = 0.001), when compared to CC. In Perio4C, there was no statistical difference between the genotypes (p > 0.05), although a lower IL-10 expression and release compared with PH CC was seen (p = 0.033 and p < 0.001). Conclusion: The rs6667202 SNP is functional in PH, as it decreases the expression and production of IL-10. In Perio4C, other factors may be masking its action by altering the IL-10's response.Periodontics Division Department of Prosthodontics and Periodontics Piracicaba Dental School University of CampinasOral Pathology Division Department of Oral Diagnosis Piracicaba Dental School University of CampinasMicrobiology and Immunology Division Department of Biosciences and Oral Biopathology São José dos Campos School of Dentistry São Paulo State UniversityPharmacology Anesthesiology and Therapeutics Division Department of Biosciences Piracicaba Dental School University of CampinasPeriodontics Division Department of Diagnosis and Surgery São José dos Campos School of Dentistry São Paulo State UniversityMicrobiology and Immunology Division Department of Biosciences and Oral Biopathology São José dos Campos School of Dentistry São Paulo State UniversityPeriodontics Division Department of Diagnosis and Surgery São José dos Campos School of Dentistry São Paulo State UniversityUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Stolf, Camila S.Sacramento, Catharina M.Paz, Hélvis E. S.Machado, Renato A.Ramos, Lucas P. [UNESP]de Oliveira, Luciane D. [UNESP]Cogo-Müller, KarinaSantamaria, Mauro P. [UNESP]Ruiz, Karina G. S.Casarin, Renato C. V.2022-04-29T08:34:56Z2022-04-29T08:34:56Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article85-93http://dx.doi.org/10.1111/jre.12940Journal of Periodontal Research, v. 57, n. 1, p. 85-93, 2022.1600-07650022-3484http://hdl.handle.net/11449/22964110.1111/jre.129402-s2.0-85116375782Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Periodontal Researchinfo:eu-repo/semantics/openAccess2022-04-29T08:34:56Zoai:repositorio.unesp.br:11449/229641Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:34:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study |
title |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study |
spellingShingle |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study Stolf, Camila S. |
title_short |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study |
title_full |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study |
title_fullStr |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study |
title_full_unstemmed |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study |
title_sort |
IL10 promoter rs6667202 polymorphism is functional in health but not in grade c periodontitis patients: A pilot study |
author |
Stolf, Camila S. |
author_facet |
Stolf, Camila S. Sacramento, Catharina M. Paz, Hélvis E. S. Machado, Renato A. Ramos, Lucas P. [UNESP] de Oliveira, Luciane D. [UNESP] Cogo-Müller, Karina Santamaria, Mauro P. [UNESP] Ruiz, Karina G. S. Casarin, Renato C. V. |
author_role |
author |
author2 |
Sacramento, Catharina M. Paz, Hélvis E. S. Machado, Renato A. Ramos, Lucas P. [UNESP] de Oliveira, Luciane D. [UNESP] Cogo-Müller, Karina Santamaria, Mauro P. [UNESP] Ruiz, Karina G. S. Casarin, Renato C. V. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Stolf, Camila S. Sacramento, Catharina M. Paz, Hélvis E. S. Machado, Renato A. Ramos, Lucas P. [UNESP] de Oliveira, Luciane D. [UNESP] Cogo-Müller, Karina Santamaria, Mauro P. [UNESP] Ruiz, Karina G. S. Casarin, Renato C. V. |
description |
Background and objective: Previous studies have demonstrated an association between the IL10 promoter rs6667202 (C > A) single-nucleotide polymorphism (SNP) and grade C, stage 3 or 4 periodontitis (Perio4C) in the Brazilian population, where the altered A allele was detected more frequently in these patients. However, no functional analysis of this variation has yet been performed. Thus, the objective of this preliminary study was to evaluate the functionality of rs6667202 in gingival fibroblasts (GFs) of individuals with Perio4C and with periodontal health (PH) stimulated with Aggregatibacter actinomycetencomitans protein extract (AaPE). Methods: Patients with PH and Perio4C were segregated according to their genotype (AA, AC, or CC), and a biopsy was performed to establish the culture of the GFs. After GFs exposure to AaPE at 5 µg/ml for 1.5 h, RNA was extracted to analyze IL10 expression by qPCR. Aliquots of the cell's supernatant were subjected to immunoenzymatic analysis (MAGpix) to detect interleukin-10 (IL-10). Results: In PH, the genotypes AA and AC are related to less expression of IL10 (p = 0.027 and p < 0.0001) and less production of IL-10 (p = 0.002 and p = 0.001), when compared to CC. In Perio4C, there was no statistical difference between the genotypes (p > 0.05), although a lower IL-10 expression and release compared with PH CC was seen (p = 0.033 and p < 0.001). Conclusion: The rs6667202 SNP is functional in PH, as it decreases the expression and production of IL-10. In Perio4C, other factors may be masking its action by altering the IL-10's response. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:34:56Z 2022-04-29T08:34:56Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/jre.12940 Journal of Periodontal Research, v. 57, n. 1, p. 85-93, 2022. 1600-0765 0022-3484 http://hdl.handle.net/11449/229641 10.1111/jre.12940 2-s2.0-85116375782 |
url |
http://dx.doi.org/10.1111/jre.12940 http://hdl.handle.net/11449/229641 |
identifier_str_mv |
Journal of Periodontal Research, v. 57, n. 1, p. 85-93, 2022. 1600-0765 0022-3484 10.1111/jre.12940 2-s2.0-85116375782 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Periodontal Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
85-93 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964488361836544 |