Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fsi.2019.10.071 http://hdl.handle.net/11449/195055 |
Resumo: | In order to understand the pathophysiology of melanomcrophage centers (MMCs) formation during the tilapia defense response to bacterial infections, the present study evaluated the response, in terms of area, number and pigment constitution, of splenic MMCs of Oreochromis niloticus subjected to intraperitoneal (i.p.) infection with Aeromonas hydrophila and Mycobacterium marinum. Eighty-four fish (396.9 +/- 21.0 g) were randomly distributed into twelve plastic tanks (300 L), to constitute three treatments with 28 animals each: control group (inoculated with PBS); Infected with A. hydrophila (1 x 10(7) UFC mL(-1)); Infected with M. marinum (1 x 10(6) UFC mL(-1)). The spleen was collected in seven fish per treatment on the 3rd, 7th, 14th and 21st day post-infection (DPI). The results revealed the participation of MMCs in the defense response of tilapia during bacterial infection by A. hydrophila and M. marinum, since there was an increase in the number and size of these cell aggregates. Variation of pigment accumulation with significant increase of hemosiderin, in infected tilapias by A. hydrophila, bacteria responsible for causing hemolytic anemia in fish was also found. On the other hand, M. marinum-infected tilapia had high amount of melanin in MMCs. In general, mycobacterial infections are notoriously difficult to treat, being characterized as a chronic disease. These findings demonstrate different strategies of fish response during the evolution of these bacterial diseases. |
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Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infectionsCichlidsHemosiderinImmunityLipofuscinMelaninOreochromis niloticusIn order to understand the pathophysiology of melanomcrophage centers (MMCs) formation during the tilapia defense response to bacterial infections, the present study evaluated the response, in terms of area, number and pigment constitution, of splenic MMCs of Oreochromis niloticus subjected to intraperitoneal (i.p.) infection with Aeromonas hydrophila and Mycobacterium marinum. Eighty-four fish (396.9 +/- 21.0 g) were randomly distributed into twelve plastic tanks (300 L), to constitute three treatments with 28 animals each: control group (inoculated with PBS); Infected with A. hydrophila (1 x 10(7) UFC mL(-1)); Infected with M. marinum (1 x 10(6) UFC mL(-1)). The spleen was collected in seven fish per treatment on the 3rd, 7th, 14th and 21st day post-infection (DPI). The results revealed the participation of MMCs in the defense response of tilapia during bacterial infection by A. hydrophila and M. marinum, since there was an increase in the number and size of these cell aggregates. Variation of pigment accumulation with significant increase of hemosiderin, in infected tilapias by A. hydrophila, bacteria responsible for causing hemolytic anemia in fish was also found. On the other hand, M. marinum-infected tilapia had high amount of melanin in MMCs. In general, mycobacterial infections are notoriously difficult to treat, being characterized as a chronic disease. These findings demonstrate different strategies of fish response during the evolution of these bacterial diseases.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Jaboticabal Campus, Sao Paulo, SP, BrazilFed Univ Rondonia, Dept Vet Med, Rolim de Moura Campus, Rolim De Moura, Rondonia, BrazilBrasil Univ, Sao Paulo, SP, BrazilBiol Inst Sao Paulo, TB Lab, Sao Paulo, BrazilSao Paulo State Univ, Jaboticabal Campus, Sao Paulo, SP, BrazilFAPESP: 13/01838-8Elsevier B.V.Universidade Estadual Paulista (Unesp)Fed Univ RondoniaBrasil UnivBiol Inst Sao PauloManrique, Wilson Gomez [UNESP]Pereira Figueiredo, Mayra AraguaiaCharlie-Silva, IvesAndrade Belo, Marco Antonio de [UNESP]Dib, Cristina Corsi2020-12-10T17:03:14Z2020-12-10T17:03:14Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article514-518http://dx.doi.org/10.1016/j.fsi.2019.10.071Fish & Shellfish Immunology. London: Academic Press Ltd- Elsevier Science Ltd, v. 95, p. 514-518, 2019.1050-4648http://hdl.handle.net/11449/19505510.1016/j.fsi.2019.10.071WOS:000503099200051Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish & Shellfish Immunologyinfo:eu-repo/semantics/openAccess2021-10-23T04:24:34Zoai:repositorio.unesp.br:11449/195055Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T04:24:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections |
title |
Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections |
spellingShingle |
Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections Manrique, Wilson Gomez [UNESP] Cichlids Hemosiderin Immunity Lipofuscin Melanin Oreochromis niloticus |
title_short |
Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections |
title_full |
Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections |
title_fullStr |
Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections |
title_full_unstemmed |
Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections |
title_sort |
Spleen melanomacrophage centers response of Nile tilapia during Aeromanas hydrophila and Mycobacterium marinum infections |
author |
Manrique, Wilson Gomez [UNESP] |
author_facet |
Manrique, Wilson Gomez [UNESP] Pereira Figueiredo, Mayra Araguaia Charlie-Silva, Ives Andrade Belo, Marco Antonio de [UNESP] Dib, Cristina Corsi |
author_role |
author |
author2 |
Pereira Figueiredo, Mayra Araguaia Charlie-Silva, Ives Andrade Belo, Marco Antonio de [UNESP] Dib, Cristina Corsi |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fed Univ Rondonia Brasil Univ Biol Inst Sao Paulo |
dc.contributor.author.fl_str_mv |
Manrique, Wilson Gomez [UNESP] Pereira Figueiredo, Mayra Araguaia Charlie-Silva, Ives Andrade Belo, Marco Antonio de [UNESP] Dib, Cristina Corsi |
dc.subject.por.fl_str_mv |
Cichlids Hemosiderin Immunity Lipofuscin Melanin Oreochromis niloticus |
topic |
Cichlids Hemosiderin Immunity Lipofuscin Melanin Oreochromis niloticus |
description |
In order to understand the pathophysiology of melanomcrophage centers (MMCs) formation during the tilapia defense response to bacterial infections, the present study evaluated the response, in terms of area, number and pigment constitution, of splenic MMCs of Oreochromis niloticus subjected to intraperitoneal (i.p.) infection with Aeromonas hydrophila and Mycobacterium marinum. Eighty-four fish (396.9 +/- 21.0 g) were randomly distributed into twelve plastic tanks (300 L), to constitute three treatments with 28 animals each: control group (inoculated with PBS); Infected with A. hydrophila (1 x 10(7) UFC mL(-1)); Infected with M. marinum (1 x 10(6) UFC mL(-1)). The spleen was collected in seven fish per treatment on the 3rd, 7th, 14th and 21st day post-infection (DPI). The results revealed the participation of MMCs in the defense response of tilapia during bacterial infection by A. hydrophila and M. marinum, since there was an increase in the number and size of these cell aggregates. Variation of pigment accumulation with significant increase of hemosiderin, in infected tilapias by A. hydrophila, bacteria responsible for causing hemolytic anemia in fish was also found. On the other hand, M. marinum-infected tilapia had high amount of melanin in MMCs. In general, mycobacterial infections are notoriously difficult to treat, being characterized as a chronic disease. These findings demonstrate different strategies of fish response during the evolution of these bacterial diseases. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2020-12-10T17:03:14Z 2020-12-10T17:03:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2019.10.071 Fish & Shellfish Immunology. London: Academic Press Ltd- Elsevier Science Ltd, v. 95, p. 514-518, 2019. 1050-4648 http://hdl.handle.net/11449/195055 10.1016/j.fsi.2019.10.071 WOS:000503099200051 |
url |
http://dx.doi.org/10.1016/j.fsi.2019.10.071 http://hdl.handle.net/11449/195055 |
identifier_str_mv |
Fish & Shellfish Immunology. London: Academic Press Ltd- Elsevier Science Ltd, v. 95, p. 514-518, 2019. 1050-4648 10.1016/j.fsi.2019.10.071 WOS:000503099200051 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish & Shellfish Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
514-518 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961656854151168 |