FOXO1 is downregulated in obese mice subjected to short-term strength training
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jcp.30882 http://hdl.handle.net/11449/237884 |
Resumo: | Obesity is a worldwide health problem and is directly associated with insulin resistance and type 2 diabetes. The liver is an important organ for the control of healthy glycemic levels, since insulin resistance in this organ reduces phosphorylation of forkhead box protein 1 (FOXO1) protein, leading to higher hepatic glucose production (HGP) and fasting hyperglycemia. Aerobic physical training is known as an important strategy in increasing the insulin action in the liver by increasing FOXO1 phosphorylation and reducing gluconeogenesis. However, little is known about the effects of strength training in this context. This study aimed to investigate the effects of short-term strength training on hepatic insulin sensitivity and glycogen synthase kinase-3 beta (GSK3 beta) and FOXO1 phosphorylation in obese (OB) mice. To achieve this goal, OB Swiss mice performed the strength training protocol (one daily session for 15 days). Short-term strength training increased the phosphorylation of protein kinase B and GSK3 beta in the liver after insulin stimulus and improved the control of HGP during the pyruvate tolerance test. On the other hand, sedentary OB animals reduced FOXO1 phosphorylation and increased the levels of nuclear FOXO1 in the liver, increasing the phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) content. The bioinformatics analysis also showed positive correlations between hepatic FOXO1 levels and gluconeogenic genes, reinforcing our findings. However, strength-trained animals reverted to this scenario, regardless of body adiposity changes. In conclusion, short-term strength training is an efficient strategy to enhance the insulin action in the liver of OB mice, contributing to glycemic control by reducing the activity of hepatic FOXO1 and lowering PEPCK and G6Pase contents. |
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FOXO1 is downregulated in obese mice subjected to short-term strength trainingDiabetesGluconeogenesisInsulin sensitivityLiverObesityShort-term strength trainingObesity is a worldwide health problem and is directly associated with insulin resistance and type 2 diabetes. The liver is an important organ for the control of healthy glycemic levels, since insulin resistance in this organ reduces phosphorylation of forkhead box protein 1 (FOXO1) protein, leading to higher hepatic glucose production (HGP) and fasting hyperglycemia. Aerobic physical training is known as an important strategy in increasing the insulin action in the liver by increasing FOXO1 phosphorylation and reducing gluconeogenesis. However, little is known about the effects of strength training in this context. This study aimed to investigate the effects of short-term strength training on hepatic insulin sensitivity and glycogen synthase kinase-3 beta (GSK3 beta) and FOXO1 phosphorylation in obese (OB) mice. To achieve this goal, OB Swiss mice performed the strength training protocol (one daily session for 15 days). Short-term strength training increased the phosphorylation of protein kinase B and GSK3 beta in the liver after insulin stimulus and improved the control of HGP during the pyruvate tolerance test. On the other hand, sedentary OB animals reduced FOXO1 phosphorylation and increased the levels of nuclear FOXO1 in the liver, increasing the phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) content. The bioinformatics analysis also showed positive correlations between hepatic FOXO1 levels and gluconeogenic genes, reinforcing our findings. However, strength-trained animals reverted to this scenario, regardless of body adiposity changes. In conclusion, short-term strength training is an efficient strategy to enhance the insulin action in the liver of OB mice, contributing to glycemic control by reducing the activity of hepatic FOXO1 and lowering PEPCK and G6Pase contents.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAEPEXUniv Estadual Campinas, Sch Appl Sci, Exercise Cell Biol Lab, Limeira, BrazilUniv Estadual Campinas, Sch Appl Sci, Lab Mol Biol Exercise, Limeira, BrazilUniv Estadual Campinas, Sch Appl Sci, Lab Nutr Genom, Nutr Div, Limeira, BrazilUniv Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, BrazilUniv Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Postgrad Program Phys Educ & Sport, Ribeirao Preto, BrazilUniv Estadual Campinas, Sch Appl Sci, Multidisciplinary Lab Food & Hlth, Hlth Div, Limeira, BrazilSao Paulo State Univ, UNESP, Sch Technol & Sci, Dept Phys Educ,Postgrad Program,Multicentr Physio, Campus Aracatuba, Presidente Prudente, BrazilState Univ Sao Paulo, UNESP, Expt Lab Exercise Biol, Presidente Prudente, BrazilUniv Estadual Campinas, Lab Cell Signaling, Obes & Comorbid Res Ctr, Campinas, BrazilUniv Fed Sao Paulo, Dept Biochem, Sao Paulo, BrazilSao Paulo State Univ, UNESP, Sch Technol & Sci, Dept Phys Educ,Postgrad Program,Multicentr Physio, Campus Aracatuba, Presidente Prudente, BrazilState Univ Sao Paulo, UNESP, Expt Lab Exercise Biol, Presidente Prudente, BrazilFAPESP: 2016/12569-6FAPESP: 2015/07199-2Wiley-BlackwellUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Federal de São Paulo (UNIFESP)Pereira, Rodrigo M.Rodrigues, Kellen C. da CruzSant'Ana, Marcella R.Rocha, Alisson L. daMorelli, Ana P.Veras, Allice S. C. [UNESP]Gaspar, Rodrigo S.Fernandes, Celio J. da CostaTeixeira, Giovana R. [UNESP]Simabuco, Fernando M.Silva, Adelino S. R. daCintra, Dennys E.Ropelle, Eduardo R.Pauli, Jose R.Moura, Leandro P. de2022-11-30T13:47:37Z2022-11-30T13:47:37Z2022-09-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13http://dx.doi.org/10.1002/jcp.30882Journal Of Cellular Physiology. Hoboken: Wiley, 13 p., 2022.0021-9541http://hdl.handle.net/11449/23788410.1002/jcp.30882WOS:000855419800001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Cellular Physiologyinfo:eu-repo/semantics/openAccess2022-11-30T13:47:37Zoai:repositorio.unesp.br:11449/237884Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-11-30T13:47:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
FOXO1 is downregulated in obese mice subjected to short-term strength training |
title |
FOXO1 is downregulated in obese mice subjected to short-term strength training |
spellingShingle |
FOXO1 is downregulated in obese mice subjected to short-term strength training Pereira, Rodrigo M. Diabetes Gluconeogenesis Insulin sensitivity Liver Obesity Short-term strength training |
title_short |
FOXO1 is downregulated in obese mice subjected to short-term strength training |
title_full |
FOXO1 is downregulated in obese mice subjected to short-term strength training |
title_fullStr |
FOXO1 is downregulated in obese mice subjected to short-term strength training |
title_full_unstemmed |
FOXO1 is downregulated in obese mice subjected to short-term strength training |
title_sort |
FOXO1 is downregulated in obese mice subjected to short-term strength training |
author |
Pereira, Rodrigo M. |
author_facet |
Pereira, Rodrigo M. Rodrigues, Kellen C. da Cruz Sant'Ana, Marcella R. Rocha, Alisson L. da Morelli, Ana P. Veras, Allice S. C. [UNESP] Gaspar, Rodrigo S. Fernandes, Celio J. da Costa Teixeira, Giovana R. [UNESP] Simabuco, Fernando M. Silva, Adelino S. R. da Cintra, Dennys E. Ropelle, Eduardo R. Pauli, Jose R. Moura, Leandro P. de |
author_role |
author |
author2 |
Rodrigues, Kellen C. da Cruz Sant'Ana, Marcella R. Rocha, Alisson L. da Morelli, Ana P. Veras, Allice S. C. [UNESP] Gaspar, Rodrigo S. Fernandes, Celio J. da Costa Teixeira, Giovana R. [UNESP] Simabuco, Fernando M. Silva, Adelino S. R. da Cintra, Dennys E. Ropelle, Eduardo R. Pauli, Jose R. Moura, Leandro P. de |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Pereira, Rodrigo M. Rodrigues, Kellen C. da Cruz Sant'Ana, Marcella R. Rocha, Alisson L. da Morelli, Ana P. Veras, Allice S. C. [UNESP] Gaspar, Rodrigo S. Fernandes, Celio J. da Costa Teixeira, Giovana R. [UNESP] Simabuco, Fernando M. Silva, Adelino S. R. da Cintra, Dennys E. Ropelle, Eduardo R. Pauli, Jose R. Moura, Leandro P. de |
dc.subject.por.fl_str_mv |
Diabetes Gluconeogenesis Insulin sensitivity Liver Obesity Short-term strength training |
topic |
Diabetes Gluconeogenesis Insulin sensitivity Liver Obesity Short-term strength training |
description |
Obesity is a worldwide health problem and is directly associated with insulin resistance and type 2 diabetes. The liver is an important organ for the control of healthy glycemic levels, since insulin resistance in this organ reduces phosphorylation of forkhead box protein 1 (FOXO1) protein, leading to higher hepatic glucose production (HGP) and fasting hyperglycemia. Aerobic physical training is known as an important strategy in increasing the insulin action in the liver by increasing FOXO1 phosphorylation and reducing gluconeogenesis. However, little is known about the effects of strength training in this context. This study aimed to investigate the effects of short-term strength training on hepatic insulin sensitivity and glycogen synthase kinase-3 beta (GSK3 beta) and FOXO1 phosphorylation in obese (OB) mice. To achieve this goal, OB Swiss mice performed the strength training protocol (one daily session for 15 days). Short-term strength training increased the phosphorylation of protein kinase B and GSK3 beta in the liver after insulin stimulus and improved the control of HGP during the pyruvate tolerance test. On the other hand, sedentary OB animals reduced FOXO1 phosphorylation and increased the levels of nuclear FOXO1 in the liver, increasing the phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase) content. The bioinformatics analysis also showed positive correlations between hepatic FOXO1 levels and gluconeogenic genes, reinforcing our findings. However, strength-trained animals reverted to this scenario, regardless of body adiposity changes. In conclusion, short-term strength training is an efficient strategy to enhance the insulin action in the liver of OB mice, contributing to glycemic control by reducing the activity of hepatic FOXO1 and lowering PEPCK and G6Pase contents. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:47:37Z 2022-11-30T13:47:37Z 2022-09-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jcp.30882 Journal Of Cellular Physiology. Hoboken: Wiley, 13 p., 2022. 0021-9541 http://hdl.handle.net/11449/237884 10.1002/jcp.30882 WOS:000855419800001 |
url |
http://dx.doi.org/10.1002/jcp.30882 http://hdl.handle.net/11449/237884 |
identifier_str_mv |
Journal Of Cellular Physiology. Hoboken: Wiley, 13 p., 2022. 0021-9541 10.1002/jcp.30882 WOS:000855419800001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Cellular Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790375543308288 |