Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fpls.2015.00561 http://hdl.handle.net/11449/158449 |
Resumo: | In Nicotiana tabacum, female gametophytes are not fully developed at anthesis, but flower buds pollinated 12 h before anthesis produce mature embryo sacs. We investigated several pollination-associated parameters in N. tabacum flower buds to determine the developmental timing of important events in preparation for successful fertilization. First, we performed hand pollinations in flowers from stages 4 to 11 to study at which developmental stage pollination would produce fruits. A Peroxtesmo test was performed to correlate peroxidase activity on the stigma surface, indicative of stigma receptivity, with fruit set. Pollen tube growth and female gametophyte development were microscopically analyzed in pistils of different developmental stages. Fruits were obtained only after pollinations of flower buds at late stage 7 and older; fruit weight and seed germination capacity increased as the developmental stage of the pollinated flower approached anthesis. Despite positive peroxidase activity and pollen tube growth, pistils at stages 5 and 6 were unable to produce fruits. At late stage 7, female gametophytes were undergoing first mitotic division. After 24 h, female gametophytes of unpollinated pistils were still in the end of the first division, whereas those of pollinated pistils showed egg cells. RT-qPCR assay showed that the expression of the NtEC1 gene, a marker of egg cell development, is considerably higher in pollinated late stage 7 ovaries compared with unpollinated ovaries. To test whether ethylene is the signal eliciting female gametophyte maturation, the expression of ACC synthase was examined in unpollinated and pollinated stage 6 and late stage 7 stigmas/styles. Pollination induced NtACS expression in stage 6 pistils, which are unable to produce fruits. Our results show that pollination is a stimulus capable of triggering female gametophyte development in immature tobacco flowers and suggests the existence of a yet undefined signal sensed by the pistil. |
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Pollination triggers female gametophyte development in immature Nicotiana tabacum flowersstigma receptivitypollen tube growthpollination signalfemale gametophyte developmentfruit weightseed germination capacityIn Nicotiana tabacum, female gametophytes are not fully developed at anthesis, but flower buds pollinated 12 h before anthesis produce mature embryo sacs. We investigated several pollination-associated parameters in N. tabacum flower buds to determine the developmental timing of important events in preparation for successful fertilization. First, we performed hand pollinations in flowers from stages 4 to 11 to study at which developmental stage pollination would produce fruits. A Peroxtesmo test was performed to correlate peroxidase activity on the stigma surface, indicative of stigma receptivity, with fruit set. Pollen tube growth and female gametophyte development were microscopically analyzed in pistils of different developmental stages. Fruits were obtained only after pollinations of flower buds at late stage 7 and older; fruit weight and seed germination capacity increased as the developmental stage of the pollinated flower approached anthesis. Despite positive peroxidase activity and pollen tube growth, pistils at stages 5 and 6 were unable to produce fruits. At late stage 7, female gametophytes were undergoing first mitotic division. After 24 h, female gametophytes of unpollinated pistils were still in the end of the first division, whereas those of pollinated pistils showed egg cells. RT-qPCR assay showed that the expression of the NtEC1 gene, a marker of egg cell development, is considerably higher in pollinated late stage 7 ovaries compared with unpollinated ovaries. To test whether ethylene is the signal eliciting female gametophyte maturation, the expression of ACC synthase was examined in unpollinated and pollinated stage 6 and late stage 7 stigmas/styles. Pollination induced NtACS expression in stage 6 pistils, which are unable to produce fruits. Our results show that pollination is a stimulus capable of triggering female gametophyte development in immature tobacco flowers and suggests the existence of a yet undefined signal sensed by the pistil.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Med Ribeirao Preto, Programa Posgrad Genet, BR-14040901 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Programa Posgrad Genet & Melhoramento Plantas, Jaboticabal, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14049 Ribeirao Preto, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Programa Posgrad Genet & Melhoramento Plantas, Jaboticabal, BrazilFrontiers Media SaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Brito, Michael S. [UNESP]Bertolino, Ligia T.Cossalter, VivianeQuiapim, Andrea C.DePaolit, Henrique C.Goldman, Gustavo H.Teixeira, Simone P.Goldman, Maria H. S.2018-11-26T15:27:42Z2018-11-26T15:27:42Z2015-07-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.3389/fpls.2015.00561Frontiers In Plant Science. Lausanne: Frontiers Media Sa, v. 6, 10 p., 2015.1664-462Xhttp://hdl.handle.net/11449/15844910.3389/fpls.2015.00561WOS:000358684900001WOS000358684900001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Plant Scienceinfo:eu-repo/semantics/openAccess2023-11-19T06:12:49Zoai:repositorio.unesp.br:11449/158449Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-19T06:12:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers |
title |
Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers |
spellingShingle |
Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers Brito, Michael S. [UNESP] stigma receptivity pollen tube growth pollination signal female gametophyte development fruit weight seed germination capacity |
title_short |
Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers |
title_full |
Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers |
title_fullStr |
Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers |
title_full_unstemmed |
Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers |
title_sort |
Pollination triggers female gametophyte development in immature Nicotiana tabacum flowers |
author |
Brito, Michael S. [UNESP] |
author_facet |
Brito, Michael S. [UNESP] Bertolino, Ligia T. Cossalter, Viviane Quiapim, Andrea C. DePaolit, Henrique C. Goldman, Gustavo H. Teixeira, Simone P. Goldman, Maria H. S. |
author_role |
author |
author2 |
Bertolino, Ligia T. Cossalter, Viviane Quiapim, Andrea C. DePaolit, Henrique C. Goldman, Gustavo H. Teixeira, Simone P. Goldman, Maria H. S. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Brito, Michael S. [UNESP] Bertolino, Ligia T. Cossalter, Viviane Quiapim, Andrea C. DePaolit, Henrique C. Goldman, Gustavo H. Teixeira, Simone P. Goldman, Maria H. S. |
dc.subject.por.fl_str_mv |
stigma receptivity pollen tube growth pollination signal female gametophyte development fruit weight seed germination capacity |
topic |
stigma receptivity pollen tube growth pollination signal female gametophyte development fruit weight seed germination capacity |
description |
In Nicotiana tabacum, female gametophytes are not fully developed at anthesis, but flower buds pollinated 12 h before anthesis produce mature embryo sacs. We investigated several pollination-associated parameters in N. tabacum flower buds to determine the developmental timing of important events in preparation for successful fertilization. First, we performed hand pollinations in flowers from stages 4 to 11 to study at which developmental stage pollination would produce fruits. A Peroxtesmo test was performed to correlate peroxidase activity on the stigma surface, indicative of stigma receptivity, with fruit set. Pollen tube growth and female gametophyte development were microscopically analyzed in pistils of different developmental stages. Fruits were obtained only after pollinations of flower buds at late stage 7 and older; fruit weight and seed germination capacity increased as the developmental stage of the pollinated flower approached anthesis. Despite positive peroxidase activity and pollen tube growth, pistils at stages 5 and 6 were unable to produce fruits. At late stage 7, female gametophytes were undergoing first mitotic division. After 24 h, female gametophytes of unpollinated pistils were still in the end of the first division, whereas those of pollinated pistils showed egg cells. RT-qPCR assay showed that the expression of the NtEC1 gene, a marker of egg cell development, is considerably higher in pollinated late stage 7 ovaries compared with unpollinated ovaries. To test whether ethylene is the signal eliciting female gametophyte maturation, the expression of ACC synthase was examined in unpollinated and pollinated stage 6 and late stage 7 stigmas/styles. Pollination induced NtACS expression in stage 6 pistils, which are unable to produce fruits. Our results show that pollination is a stimulus capable of triggering female gametophyte development in immature tobacco flowers and suggests the existence of a yet undefined signal sensed by the pistil. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-22 2018-11-26T15:27:42Z 2018-11-26T15:27:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fpls.2015.00561 Frontiers In Plant Science. Lausanne: Frontiers Media Sa, v. 6, 10 p., 2015. 1664-462X http://hdl.handle.net/11449/158449 10.3389/fpls.2015.00561 WOS:000358684900001 WOS000358684900001.pdf |
url |
http://dx.doi.org/10.3389/fpls.2015.00561 http://hdl.handle.net/11449/158449 |
identifier_str_mv |
Frontiers In Plant Science. Lausanne: Frontiers Media Sa, v. 6, 10 p., 2015. 1664-462X 10.3389/fpls.2015.00561 WOS:000358684900001 WOS000358684900001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Plant Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media Sa |
publisher.none.fl_str_mv |
Frontiers Media Sa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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