Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/microorganisms11051145 http://hdl.handle.net/11449/249996 |
Resumo: | Anopheles darlingi is a major malaria vector in the Amazon region and, like other vectors, harbors a community of microorganisms with which it shares a network of interactions. Here, we describe the diversity and bacterial composition from the midguts and salivary glands of lab-reared and field-captured An. darlingi using metagenome sequencing of the 16S rRNA gene. The libraries were built using the amplification of the region V3–V4 16S rRNA gene. The bacterial community from the salivary glands was more diverse and richer than the community from the midguts. However, the salivary glands and midguts only showed dissimilarities in beta diversity between lab-reared mosquitoes. Despite that, intra-variability was observed in the samples. Acinetobacter and Pseudomonas were dominant in the tissues of lab-reared mosquitoes. Sequences of Wolbachia and Asaia were both found in the tissue of lab-reared mosquitoes; however, only Asaia was found in field-captured An. darlingi, but in low abundance. This is the first report on the characterization of microbiota composition from the salivary glands of An. darlingi from lab-reared and field-captured individuals. This study can provide invaluable insights for future investigations regarding mosquito development and interaction between mosquito microbiota and Plasmodium sp. |
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Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland16S rRNAAmazon regionbacteriamalaria vectormicrobiotamidgutsalivary glandAnopheles darlingi is a major malaria vector in the Amazon region and, like other vectors, harbors a community of microorganisms with which it shares a network of interactions. Here, we describe the diversity and bacterial composition from the midguts and salivary glands of lab-reared and field-captured An. darlingi using metagenome sequencing of the 16S rRNA gene. The libraries were built using the amplification of the region V3–V4 16S rRNA gene. The bacterial community from the salivary glands was more diverse and richer than the community from the midguts. However, the salivary glands and midguts only showed dissimilarities in beta diversity between lab-reared mosquitoes. Despite that, intra-variability was observed in the samples. Acinetobacter and Pseudomonas were dominant in the tissues of lab-reared mosquitoes. Sequences of Wolbachia and Asaia were both found in the tissue of lab-reared mosquitoes; however, only Asaia was found in field-captured An. darlingi, but in low abundance. This is the first report on the characterization of microbiota composition from the salivary glands of An. darlingi from lab-reared and field-captured individuals. This study can provide invaluable insights for future investigations regarding mosquito development and interaction between mosquito microbiota and Plasmodium sp.Bill and Melinda Gates FoundationPrograma de Pós-Graduação em Biologia Experimental Departament of Medicine Fundação Universidade Federal de Rondônia/Fiocruz Rondônia, ROPlataforma de Produção e Infecção de Vetores da Malária-PIVEM Laboratório de Entomologia Fiocruz Rondônia, ROMultiuser Central Laboratory Department of Bioprocesses and Biotechnology School of Agricultural Sciences São Paulo State University (UNESP), SPDepartment of Biotecnology (IBTEC–Campus Botucatu) Instituto de Biotecnologia da UNESP Universidade Estadual Paulista (UNESP), SPPrograma de Pós-Graduação em Conservação e uso de Recursos Naturais–PPGReN Departament of Biology Fundação Universidade Federal de Rondônia, Campus José Ribeiro Filho, ROLaboratório de Pesquisa Translacional e Clínica Centro de Pesquisa em Medicina Tropical, ROMultiuser Central Laboratory Department of Bioprocesses and Biotechnology School of Agricultural Sciences São Paulo State University (UNESP), SPDepartment of Biotecnology (IBTEC–Campus Botucatu) Instituto de Biotecnologia da UNESP Universidade Estadual Paulista (UNESP), SPBill and Melinda Gates Foundation: INV-003970Fundação Universidade Federal de Rondônia/Fiocruz RondôniaFiocruz RondôniaUniversidade Estadual Paulista (UNESP)Fundação Universidade Federal de RondôniaCentro de Pesquisa em Medicina TropicalSantos, Najara Akira Costa dosCarvalho, Vanessa Rafaela de [UNESP]Souza-Neto, Jayme A. [UNESP]Alonso, Diego Peres [UNESP]Ribolla, Paulo Eduardo Martins [UNESP]Medeiros, Jansen FernandesAraujo, Maisa da Silva2023-07-29T16:14:57Z2023-07-29T16:14:57Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/microorganisms11051145Microorganisms, v. 11, n. 5, 2023.2076-2607http://hdl.handle.net/11449/24999610.3390/microorganisms110511452-s2.0-85160402189Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroorganismsinfo:eu-repo/semantics/openAccess2023-07-29T16:14:57Zoai:repositorio.unesp.br:11449/249996Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:14:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland |
title |
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland |
spellingShingle |
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland Santos, Najara Akira Costa dos 16S rRNA Amazon region bacteria malaria vector microbiota midgut salivary gland |
title_short |
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland |
title_full |
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland |
title_fullStr |
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland |
title_full_unstemmed |
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland |
title_sort |
Bacterial Microbiota from Lab-Reared and Field-Captured Anopheles darlingi Midgut and Salivary Gland |
author |
Santos, Najara Akira Costa dos |
author_facet |
Santos, Najara Akira Costa dos Carvalho, Vanessa Rafaela de [UNESP] Souza-Neto, Jayme A. [UNESP] Alonso, Diego Peres [UNESP] Ribolla, Paulo Eduardo Martins [UNESP] Medeiros, Jansen Fernandes Araujo, Maisa da Silva |
author_role |
author |
author2 |
Carvalho, Vanessa Rafaela de [UNESP] Souza-Neto, Jayme A. [UNESP] Alonso, Diego Peres [UNESP] Ribolla, Paulo Eduardo Martins [UNESP] Medeiros, Jansen Fernandes Araujo, Maisa da Silva |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Fundação Universidade Federal de Rondônia/Fiocruz Rondônia Fiocruz Rondônia Universidade Estadual Paulista (UNESP) Fundação Universidade Federal de Rondônia Centro de Pesquisa em Medicina Tropical |
dc.contributor.author.fl_str_mv |
Santos, Najara Akira Costa dos Carvalho, Vanessa Rafaela de [UNESP] Souza-Neto, Jayme A. [UNESP] Alonso, Diego Peres [UNESP] Ribolla, Paulo Eduardo Martins [UNESP] Medeiros, Jansen Fernandes Araujo, Maisa da Silva |
dc.subject.por.fl_str_mv |
16S rRNA Amazon region bacteria malaria vector microbiota midgut salivary gland |
topic |
16S rRNA Amazon region bacteria malaria vector microbiota midgut salivary gland |
description |
Anopheles darlingi is a major malaria vector in the Amazon region and, like other vectors, harbors a community of microorganisms with which it shares a network of interactions. Here, we describe the diversity and bacterial composition from the midguts and salivary glands of lab-reared and field-captured An. darlingi using metagenome sequencing of the 16S rRNA gene. The libraries were built using the amplification of the region V3–V4 16S rRNA gene. The bacterial community from the salivary glands was more diverse and richer than the community from the midguts. However, the salivary glands and midguts only showed dissimilarities in beta diversity between lab-reared mosquitoes. Despite that, intra-variability was observed in the samples. Acinetobacter and Pseudomonas were dominant in the tissues of lab-reared mosquitoes. Sequences of Wolbachia and Asaia were both found in the tissue of lab-reared mosquitoes; however, only Asaia was found in field-captured An. darlingi, but in low abundance. This is the first report on the characterization of microbiota composition from the salivary glands of An. darlingi from lab-reared and field-captured individuals. This study can provide invaluable insights for future investigations regarding mosquito development and interaction between mosquito microbiota and Plasmodium sp. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:14:57Z 2023-07-29T16:14:57Z 2023-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/microorganisms11051145 Microorganisms, v. 11, n. 5, 2023. 2076-2607 http://hdl.handle.net/11449/249996 10.3390/microorganisms11051145 2-s2.0-85160402189 |
url |
http://dx.doi.org/10.3390/microorganisms11051145 http://hdl.handle.net/11449/249996 |
identifier_str_mv |
Microorganisms, v. 11, n. 5, 2023. 2076-2607 10.3390/microorganisms11051145 2-s2.0-85160402189 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microorganisms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964522550657024 |