Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fsi.2019.01.044 http://hdl.handle.net/11449/188809 |
Resumo: | The pathogenesis of sepsis involves complex systems and multiple interrelationships between the host and pathogen producing high mortality rates in various animal species. In this study, hematological disturbances, innate immunity and survival during the septic process in Piaractus mesopotamicus inoculated with Aeromonas hydrophila were studied. For this aim, fish blood samples were taken from control and infected groups 1, 3, 6, and 9 h post-inoculation (HPI). Leukogram showed reduction in the number of leukocytes and thrombocytes, followed by cessation of leukocyte chemotaxis 6 HPI and severe morphological changes in leukocytes and erythrocytes. At 3 HPI production of reactive oxygen species increased and at 6 HPI decreased. There was no change in serum lysozyme concentration and lytic activity of the complement system, despite the progressive increase in serum lytic activity and bacterial agglutination. Finally, the changes in clinical signs due to aeromonosis and increasing septicemia resulted in a reduction in survival to 57.14% after 36 HPI. It was possible concluded that these hematological and immune are crucial event in the worsening of sepsis in P. mesopotamicus, and these findings are utility for diagnosing and understanding the pathophysiology sepsis in pacu induced by A. hydrophila. |
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Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophilaBacteriaBloodInnate immunityLeukocytesPacuThe pathogenesis of sepsis involves complex systems and multiple interrelationships between the host and pathogen producing high mortality rates in various animal species. In this study, hematological disturbances, innate immunity and survival during the septic process in Piaractus mesopotamicus inoculated with Aeromonas hydrophila were studied. For this aim, fish blood samples were taken from control and infected groups 1, 3, 6, and 9 h post-inoculation (HPI). Leukogram showed reduction in the number of leukocytes and thrombocytes, followed by cessation of leukocyte chemotaxis 6 HPI and severe morphological changes in leukocytes and erythrocytes. At 3 HPI production of reactive oxygen species increased and at 6 HPI decreased. There was no change in serum lysozyme concentration and lytic activity of the complement system, despite the progressive increase in serum lytic activity and bacterial agglutination. Finally, the changes in clinical signs due to aeromonosis and increasing septicemia resulted in a reduction in survival to 57.14% after 36 HPI. It was possible concluded that these hematological and immune are crucial event in the worsening of sepsis in P. mesopotamicus, and these findings are utility for diagnosing and understanding the pathophysiology sepsis in pacu induced by A. hydrophila.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Veterinarian Pathology Faculty of Agrarian and Veterinarian Sciences São Paulo State University UnespInstitute of Biodiversity and Forests Federal University of Western Pará UFOPA ParáAquaculture Center of UNESP, JaboticabalClinical Analysis Laboratory Veterinary Hospital Federal University of Uberlândia (UFU)Department of Clinical Toxicological and Bromatological Analyses Ribeirão Preto School of Pharmaceutical Sciences University of São Paulo (USP)Department of Veterinarian Pathology Faculty of Agrarian and Veterinarian Sciences São Paulo State University UnespAquaculture Center of UNESP, JaboticabalFAPESP: 2011/20280-2FAPESP: 2014/10231-2CNPq: 441054/2014-5Universidade Estadual Paulista (Unesp)ParáUniversidade Federal de Uberlândia (UFU)Universidade de São Paulo (USP)Claudiano, Gustavo S. [UNESP]Yunis-Aguinaga, Jefferson [UNESP]Marinho-Neto, Fausto A. [UNESP]Miranda, Renata L.Martins, Isabela M. [UNESP]Otani, Fabrizia S.Mundim, Antonio V.Marzocchi-Machado, Cleni M.Moraes, Julieta R.E. [UNESP]de Moraes, Flávio Ruas [UNESP]2019-10-06T16:19:52Z2019-10-06T16:19:52Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article259-265http://dx.doi.org/10.1016/j.fsi.2019.01.044Fish and Shellfish Immunology, v. 88, p. 259-265.1095-99471050-4648http://hdl.handle.net/11449/18880910.1016/j.fsi.2019.01.0442-s2.0-85062516579Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccess2024-04-09T15:43:16Zoai:repositorio.unesp.br:11449/188809Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:43:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila |
title |
Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila |
spellingShingle |
Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila Claudiano, Gustavo S. [UNESP] Bacteria Blood Innate immunity Leukocytes Pacu |
title_short |
Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila |
title_full |
Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila |
title_fullStr |
Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila |
title_full_unstemmed |
Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila |
title_sort |
Hematological and immune changes in Piaractus mesopotamicus in the sepsis induced by Aeromonas hydrophila |
author |
Claudiano, Gustavo S. [UNESP] |
author_facet |
Claudiano, Gustavo S. [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Marinho-Neto, Fausto A. [UNESP] Miranda, Renata L. Martins, Isabela M. [UNESP] Otani, Fabrizia S. Mundim, Antonio V. Marzocchi-Machado, Cleni M. Moraes, Julieta R.E. [UNESP] de Moraes, Flávio Ruas [UNESP] |
author_role |
author |
author2 |
Yunis-Aguinaga, Jefferson [UNESP] Marinho-Neto, Fausto A. [UNESP] Miranda, Renata L. Martins, Isabela M. [UNESP] Otani, Fabrizia S. Mundim, Antonio V. Marzocchi-Machado, Cleni M. Moraes, Julieta R.E. [UNESP] de Moraes, Flávio Ruas [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Pará Universidade Federal de Uberlândia (UFU) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Claudiano, Gustavo S. [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Marinho-Neto, Fausto A. [UNESP] Miranda, Renata L. Martins, Isabela M. [UNESP] Otani, Fabrizia S. Mundim, Antonio V. Marzocchi-Machado, Cleni M. Moraes, Julieta R.E. [UNESP] de Moraes, Flávio Ruas [UNESP] |
dc.subject.por.fl_str_mv |
Bacteria Blood Innate immunity Leukocytes Pacu |
topic |
Bacteria Blood Innate immunity Leukocytes Pacu |
description |
The pathogenesis of sepsis involves complex systems and multiple interrelationships between the host and pathogen producing high mortality rates in various animal species. In this study, hematological disturbances, innate immunity and survival during the septic process in Piaractus mesopotamicus inoculated with Aeromonas hydrophila were studied. For this aim, fish blood samples were taken from control and infected groups 1, 3, 6, and 9 h post-inoculation (HPI). Leukogram showed reduction in the number of leukocytes and thrombocytes, followed by cessation of leukocyte chemotaxis 6 HPI and severe morphological changes in leukocytes and erythrocytes. At 3 HPI production of reactive oxygen species increased and at 6 HPI decreased. There was no change in serum lysozyme concentration and lytic activity of the complement system, despite the progressive increase in serum lytic activity and bacterial agglutination. Finally, the changes in clinical signs due to aeromonosis and increasing septicemia resulted in a reduction in survival to 57.14% after 36 HPI. It was possible concluded that these hematological and immune are crucial event in the worsening of sepsis in P. mesopotamicus, and these findings are utility for diagnosing and understanding the pathophysiology sepsis in pacu induced by A. hydrophila. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:19:52Z 2019-10-06T16:19:52Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2019.01.044 Fish and Shellfish Immunology, v. 88, p. 259-265. 1095-9947 1050-4648 http://hdl.handle.net/11449/188809 10.1016/j.fsi.2019.01.044 2-s2.0-85062516579 |
url |
http://dx.doi.org/10.1016/j.fsi.2019.01.044 http://hdl.handle.net/11449/188809 |
identifier_str_mv |
Fish and Shellfish Immunology, v. 88, p. 259-265. 1095-9947 1050-4648 10.1016/j.fsi.2019.01.044 2-s2.0-85062516579 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish and Shellfish Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
259-265 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965492455145472 |