Teneurin-2 presence in rat and human odontoblasts
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0184794 http://hdl.handle.net/11449/163298 |
Resumo: | Teneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed in the central nervous system during development. All teneurins contain a bioactive peptide in their carboxyl terminal named teneurin C-terminal associated peptide (TCAP). The present study analyzed the detailed distribution of teneurin-2-like immunoreactive (Ten-2-LI) cells in developing and mature rat molar teeth, as well as in mature human dental pulps. Ten-2 and TCAP-2 genic expressions were also evaluated in rat and human dental pulps. Finally, Ten-2-LI cells were analyzed during the repair process after dentinpulp complex injury in rat lower molar teeth. For this, histological sections of rat molar teeth and human dental pulps were submitted to immunohistochemical techniques, while total RNA from developing rat teeth and mature human dental pulps were submitted to conventional RT-PCR. Ten-2-LI cells were evident in the initial bell stage of rat molar teeth development, especially in ectomesenchymal cells of the dental papilla. Ten-2-LI odontoblasts showed strong immunoreactivity in rat and human mature teeth. Ten-2 and TCAP-2 genic expressions were confirmed in rat and human dental pulps. Dentin-pulp complex injury resulted in a decrease of Ten-2-LI odontoblasts after traumatic injury. Interestingly, Ten-2-LI cells were also evident in the pulp cell-rich zone in all postoperative days. In conclusion, Ten-2-LI presence in rat and human odontoblasts was demonstrated for the first time and Ten-2/TCAP-2 genic expressions were confirmed in rat and human dental pulps. Furthermore, it was revealed that Ten-2-LI rat odontoblasts can be modulated during the regenerative process. |
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Teneurin-2 presence in rat and human odontoblastsTeneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed in the central nervous system during development. All teneurins contain a bioactive peptide in their carboxyl terminal named teneurin C-terminal associated peptide (TCAP). The present study analyzed the detailed distribution of teneurin-2-like immunoreactive (Ten-2-LI) cells in developing and mature rat molar teeth, as well as in mature human dental pulps. Ten-2 and TCAP-2 genic expressions were also evaluated in rat and human dental pulps. Finally, Ten-2-LI cells were analyzed during the repair process after dentinpulp complex injury in rat lower molar teeth. For this, histological sections of rat molar teeth and human dental pulps were submitted to immunohistochemical techniques, while total RNA from developing rat teeth and mature human dental pulps were submitted to conventional RT-PCR. Ten-2-LI cells were evident in the initial bell stage of rat molar teeth development, especially in ectomesenchymal cells of the dental papilla. Ten-2-LI odontoblasts showed strong immunoreactivity in rat and human mature teeth. Ten-2 and TCAP-2 genic expressions were confirmed in rat and human dental pulps. Dentin-pulp complex injury resulted in a decrease of Ten-2-LI odontoblasts after traumatic injury. Interestingly, Ten-2-LI cells were also evident in the pulp cell-rich zone in all postoperative days. In conclusion, Ten-2-LI presence in rat and human odontoblasts was demonstrated for the first time and Ten-2/TCAP-2 genic expressions were confirmed in rat and human dental pulps. Furthermore, it was revealed that Ten-2-LI rat odontoblasts can be modulated during the regenerative process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Inst Biosci Botucatu, Botucatu, SP, BrazilSao Paulo State Univ, Sch Dent Aracatuba, Basic Sci Dept, Aracatuba, SP, BrazilSao Paulo State Univ, Sch Dent Aracatuba, Restorat Dent Dept, Aracatuba, SP, BrazilSao Paulo State Univ, Sch Dent Aracatuba, Dept Surg & Integrated Clin, Aracatuba, SP, BrazilUniv Toronto, Cell & Syst Biol Dept, Toronto, ON, CanadaSao Paulo State Univ, Inst Biosci Botucatu, Botucatu, SP, BrazilSao Paulo State Univ, Sch Dent Aracatuba, Basic Sci Dept, Aracatuba, SP, BrazilSao Paulo State Univ, Sch Dent Aracatuba, Restorat Dent Dept, Aracatuba, SP, BrazilSao Paulo State Univ, Sch Dent Aracatuba, Dept Surg & Integrated Clin, Aracatuba, SP, BrazilFAPESP: 2012/03067-6FAPESP: FAPESP 2012/08833-9FAPESP: FAPESP 2013/0635-06FAPESP: FAPESP 2013/01288-8Public Library ScienceUniversidade Estadual Paulista (Unesp)Univ TorontoTorres-da-Silvali, K. R. [UNESP]Tessarin, G. W. L. [UNESP]Dias, C. A. [UNESP]Guiati, I. Z. [UNESP]Ervolino, E. [UNESP]Goncalves, A. [UNESP]Beneti, I. M. [UNESP]Lovejoy, D. A.Casatti, C. A. [UNESP]2018-11-26T17:40:52Z2018-11-26T17:40:52Z2017-09-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19application/pdfhttp://dx.doi.org/10.1371/journal.pone.0184794Plos One. San Francisco: Public Library Science, v. 12, n. 9, 19 p., 2017.1932-6203http://hdl.handle.net/11449/16329810.1371/journal.pone.0184794WOS:000411166600042WOS000411166600042.pdf44080955173468460000-0003-4859-058384874626269318770000-0001-5650-7343Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2023-12-19T06:18:38Zoai:repositorio.unesp.br:11449/163298Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-19T06:18:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Teneurin-2 presence in rat and human odontoblasts |
title |
Teneurin-2 presence in rat and human odontoblasts |
spellingShingle |
Teneurin-2 presence in rat and human odontoblasts Torres-da-Silvali, K. R. [UNESP] |
title_short |
Teneurin-2 presence in rat and human odontoblasts |
title_full |
Teneurin-2 presence in rat and human odontoblasts |
title_fullStr |
Teneurin-2 presence in rat and human odontoblasts |
title_full_unstemmed |
Teneurin-2 presence in rat and human odontoblasts |
title_sort |
Teneurin-2 presence in rat and human odontoblasts |
author |
Torres-da-Silvali, K. R. [UNESP] |
author_facet |
Torres-da-Silvali, K. R. [UNESP] Tessarin, G. W. L. [UNESP] Dias, C. A. [UNESP] Guiati, I. Z. [UNESP] Ervolino, E. [UNESP] Goncalves, A. [UNESP] Beneti, I. M. [UNESP] Lovejoy, D. A. Casatti, C. A. [UNESP] |
author_role |
author |
author2 |
Tessarin, G. W. L. [UNESP] Dias, C. A. [UNESP] Guiati, I. Z. [UNESP] Ervolino, E. [UNESP] Goncalves, A. [UNESP] Beneti, I. M. [UNESP] Lovejoy, D. A. Casatti, C. A. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Toronto |
dc.contributor.author.fl_str_mv |
Torres-da-Silvali, K. R. [UNESP] Tessarin, G. W. L. [UNESP] Dias, C. A. [UNESP] Guiati, I. Z. [UNESP] Ervolino, E. [UNESP] Goncalves, A. [UNESP] Beneti, I. M. [UNESP] Lovejoy, D. A. Casatti, C. A. [UNESP] |
description |
Teneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed in the central nervous system during development. All teneurins contain a bioactive peptide in their carboxyl terminal named teneurin C-terminal associated peptide (TCAP). The present study analyzed the detailed distribution of teneurin-2-like immunoreactive (Ten-2-LI) cells in developing and mature rat molar teeth, as well as in mature human dental pulps. Ten-2 and TCAP-2 genic expressions were also evaluated in rat and human dental pulps. Finally, Ten-2-LI cells were analyzed during the repair process after dentinpulp complex injury in rat lower molar teeth. For this, histological sections of rat molar teeth and human dental pulps were submitted to immunohistochemical techniques, while total RNA from developing rat teeth and mature human dental pulps were submitted to conventional RT-PCR. Ten-2-LI cells were evident in the initial bell stage of rat molar teeth development, especially in ectomesenchymal cells of the dental papilla. Ten-2-LI odontoblasts showed strong immunoreactivity in rat and human mature teeth. Ten-2 and TCAP-2 genic expressions were confirmed in rat and human dental pulps. Dentin-pulp complex injury resulted in a decrease of Ten-2-LI odontoblasts after traumatic injury. Interestingly, Ten-2-LI cells were also evident in the pulp cell-rich zone in all postoperative days. In conclusion, Ten-2-LI presence in rat and human odontoblasts was demonstrated for the first time and Ten-2/TCAP-2 genic expressions were confirmed in rat and human dental pulps. Furthermore, it was revealed that Ten-2-LI rat odontoblasts can be modulated during the regenerative process. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-19 2018-11-26T17:40:52Z 2018-11-26T17:40:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0184794 Plos One. San Francisco: Public Library Science, v. 12, n. 9, 19 p., 2017. 1932-6203 http://hdl.handle.net/11449/163298 10.1371/journal.pone.0184794 WOS:000411166600042 WOS000411166600042.pdf 4408095517346846 0000-0003-4859-0583 8487462626931877 0000-0001-5650-7343 |
url |
http://dx.doi.org/10.1371/journal.pone.0184794 http://hdl.handle.net/11449/163298 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 12, n. 9, 19 p., 2017. 1932-6203 10.1371/journal.pone.0184794 WOS:000411166600042 WOS000411166600042.pdf 4408095517346846 0000-0003-4859-0583 8487462626931877 0000-0001-5650-7343 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Plos One 1,164 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
19 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
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Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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