Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1806-83242006000200006 http://hdl.handle.net/11449/68822 |
Resumo: | This study aimed at evaluating the antisepsis of the root canal system (RCS) and periapical region (PR) provided by rotary instrumentation associated with chlorhexidine + calcium hydroxide as intracanal medicament. Chronic periapical lesions were induced in 26 pre-molar roots in two dogs. After microbiological sampling, automatic instrumentation using the Profile system and irrigation with 5.25% sodium hypochlorite solution, with a final rinse of 14.3% EDTA followed by profuse irrigation with physiological saline were carried out in 18 root canals. After drying the canals, a paste based on calcium hydroxide associated with a 2% chlorhexidine digluconate solution was placed inside them. After 21 days, the medication was removed, leaving the root canals empty and coronally sealed. After 96 hours, a final microbiological sample was obtained, followed by histomicrobiological processing by the Brown & Brenn method. Eight untreated root canals represented the control group (C-G). Based on the Mann-Whitney test at a confidence level of 5% (p < 0.05), the procedures of antisepsis used offered significant efficacy (p < 0.05) resulting in 100.0% of the canals free of microorganisms. In the C-G, an elevated incidence of various microbial morphotypes was confirmed in all sites of the RCS, with the presence of microbial colonies in the periapical region. In contrast, the experimental group showed a similar pattern of infection in the RCS, although less intense and a reduced level of periapical infection (p < 0.05). It was concluded that adequate instrumentation followed by the application of calcium hydroxide + chlorhexidine offered significant elimination of microorganisms. |
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Repositório Institucional da UNESP |
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Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogsCalcium hydroxideChlorhexidineEndodonticsMicrobiologybone cementcalcium hydroxidechlorhexidinetopical antiinfective agentanimalanimal modeldogdrug combinationendodonticsinstrumentationmicrobiologytooth pulpAnimalsAnti-Infective Agents, LocalBone CementsCalcium HydroxideDental Pulp CavityDogsDrug CombinationsModels, AnimalRoot Canal PreparationThis study aimed at evaluating the antisepsis of the root canal system (RCS) and periapical region (PR) provided by rotary instrumentation associated with chlorhexidine + calcium hydroxide as intracanal medicament. Chronic periapical lesions were induced in 26 pre-molar roots in two dogs. After microbiological sampling, automatic instrumentation using the Profile system and irrigation with 5.25% sodium hypochlorite solution, with a final rinse of 14.3% EDTA followed by profuse irrigation with physiological saline were carried out in 18 root canals. After drying the canals, a paste based on calcium hydroxide associated with a 2% chlorhexidine digluconate solution was placed inside them. After 21 days, the medication was removed, leaving the root canals empty and coronally sealed. After 96 hours, a final microbiological sample was obtained, followed by histomicrobiological processing by the Brown & Brenn method. Eight untreated root canals represented the control group (C-G). Based on the Mann-Whitney test at a confidence level of 5% (p < 0.05), the procedures of antisepsis used offered significant efficacy (p < 0.05) resulting in 100.0% of the canals free of microorganisms. In the C-G, an elevated incidence of various microbial morphotypes was confirmed in all sites of the RCS, with the presence of microbial colonies in the periapical region. In contrast, the experimental group showed a similar pattern of infection in the RCS, although less intense and a reduced level of periapical infection (p < 0.05). It was concluded that adequate instrumentation followed by the application of calcium hydroxide + chlorhexidine offered significant elimination of microorganisms.Department of Endodontics Federal University of the Valleys of Jequitinhonha and Mucuri, Minas GeraisDepartment of Endodontics School of Dentistry of Araraquara São Paulo State University, São PauloDepartment of Pediatric Dentistry School of Dentistry of Ribeirão Preto, Ribeirão Preto, SPDepartment of Microbiology School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo, São PauloDepartment of Endodontics School of Dentistry of Araraquara São Paulo State University, São PauloFederal University of the Valleys of Jequitinhonha and MucuriUniversidade Estadual Paulista (Unesp)School of Dentistry of Ribeirão PretoUniversidade de São Paulo (USP)Soares, Janir AlvesLeonardo, Mario Roberto [UNESP]da Silva, Léa Assed Bezerra [UNESP]Tanomaru Filho, Mario [UNESP]Ito, Izabel Yoko2014-05-27T11:21:50Z2014-05-27T11:21:50Z2006-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article120-126application/pdfhttp://dx.doi.org/10.1590/S1806-83242006000200006Brazilian Oral Research, v. 20, n. 2, p. 120-126, 2006.1806-83241807-3107http://hdl.handle.net/11449/6882210.1590/S1806-83242006000200006S1806-832420060002000062-s2.0-337463629242-s2.0-33746362924.pdf2665211298584751Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Research1.223info:eu-repo/semantics/openAccess2023-12-09T06:19:30Zoai:repositorio.unesp.br:11449/68822Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-09T06:19:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs |
title |
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs |
spellingShingle |
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs Soares, Janir Alves Calcium hydroxide Chlorhexidine Endodontics Microbiology bone cement calcium hydroxide chlorhexidine topical antiinfective agent animal animal model dog drug combination endodontics instrumentation microbiology tooth pulp Animals Anti-Infective Agents, Local Bone Cements Calcium Hydroxide Dental Pulp Cavity Dogs Drug Combinations Models, Animal Root Canal Preparation |
title_short |
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs |
title_full |
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs |
title_fullStr |
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs |
title_full_unstemmed |
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs |
title_sort |
Effect of rotary instrumentation and of the association of calcium hydroxide and chlorhexidine on the antisepsis of the root canal system in dogs |
author |
Soares, Janir Alves |
author_facet |
Soares, Janir Alves Leonardo, Mario Roberto [UNESP] da Silva, Léa Assed Bezerra [UNESP] Tanomaru Filho, Mario [UNESP] Ito, Izabel Yoko |
author_role |
author |
author2 |
Leonardo, Mario Roberto [UNESP] da Silva, Léa Assed Bezerra [UNESP] Tanomaru Filho, Mario [UNESP] Ito, Izabel Yoko |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Federal University of the Valleys of Jequitinhonha and Mucuri Universidade Estadual Paulista (Unesp) School of Dentistry of Ribeirão Preto Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Soares, Janir Alves Leonardo, Mario Roberto [UNESP] da Silva, Léa Assed Bezerra [UNESP] Tanomaru Filho, Mario [UNESP] Ito, Izabel Yoko |
dc.subject.por.fl_str_mv |
Calcium hydroxide Chlorhexidine Endodontics Microbiology bone cement calcium hydroxide chlorhexidine topical antiinfective agent animal animal model dog drug combination endodontics instrumentation microbiology tooth pulp Animals Anti-Infective Agents, Local Bone Cements Calcium Hydroxide Dental Pulp Cavity Dogs Drug Combinations Models, Animal Root Canal Preparation |
topic |
Calcium hydroxide Chlorhexidine Endodontics Microbiology bone cement calcium hydroxide chlorhexidine topical antiinfective agent animal animal model dog drug combination endodontics instrumentation microbiology tooth pulp Animals Anti-Infective Agents, Local Bone Cements Calcium Hydroxide Dental Pulp Cavity Dogs Drug Combinations Models, Animal Root Canal Preparation |
description |
This study aimed at evaluating the antisepsis of the root canal system (RCS) and periapical region (PR) provided by rotary instrumentation associated with chlorhexidine + calcium hydroxide as intracanal medicament. Chronic periapical lesions were induced in 26 pre-molar roots in two dogs. After microbiological sampling, automatic instrumentation using the Profile system and irrigation with 5.25% sodium hypochlorite solution, with a final rinse of 14.3% EDTA followed by profuse irrigation with physiological saline were carried out in 18 root canals. After drying the canals, a paste based on calcium hydroxide associated with a 2% chlorhexidine digluconate solution was placed inside them. After 21 days, the medication was removed, leaving the root canals empty and coronally sealed. After 96 hours, a final microbiological sample was obtained, followed by histomicrobiological processing by the Brown & Brenn method. Eight untreated root canals represented the control group (C-G). Based on the Mann-Whitney test at a confidence level of 5% (p < 0.05), the procedures of antisepsis used offered significant efficacy (p < 0.05) resulting in 100.0% of the canals free of microorganisms. In the C-G, an elevated incidence of various microbial morphotypes was confirmed in all sites of the RCS, with the presence of microbial colonies in the periapical region. In contrast, the experimental group showed a similar pattern of infection in the RCS, although less intense and a reduced level of periapical infection (p < 0.05). It was concluded that adequate instrumentation followed by the application of calcium hydroxide + chlorhexidine offered significant elimination of microorganisms. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-04-01 2014-05-27T11:21:50Z 2014-05-27T11:21:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1806-83242006000200006 Brazilian Oral Research, v. 20, n. 2, p. 120-126, 2006. 1806-8324 1807-3107 http://hdl.handle.net/11449/68822 10.1590/S1806-83242006000200006 S1806-83242006000200006 2-s2.0-33746362924 2-s2.0-33746362924.pdf 2665211298584751 |
url |
http://dx.doi.org/10.1590/S1806-83242006000200006 http://hdl.handle.net/11449/68822 |
identifier_str_mv |
Brazilian Oral Research, v. 20, n. 2, p. 120-126, 2006. 1806-8324 1807-3107 10.1590/S1806-83242006000200006 S1806-83242006000200006 2-s2.0-33746362924 2-s2.0-33746362924.pdf 2665211298584751 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research 1.223 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
120-126 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965223686242304 |