In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-9946201658048 http://hdl.handle.net/11449/161780 |
Resumo: | Thirty-eight dogs naturally affected by visceral leishmaniasis were recruited in Aracatuba, Sao Paulo State, Brazil - an endemic area for visceral leishmaniasis. The animals were distributed into one of two groups, according to their clinical and laboratory features, as either symptomatic or asymptomatic dogs. Correlations between clinical features and inflammatory patterns, cellular immune responses, and parasitism in the macroscopically uninjured skin of the ear were investigated. Histological skin patterns were similar in both groups, and were generally characterized by a mild to intense inflammatory infiltrate in the dermis, mainly consisting of mononuclear cells. There was no difference in the number of parasites in the skin (amastigotes/mm(2)) between the two groups. Concerning the characterization of the cellular immune response, the number of positive inducible nitric oxide synthase (iNOS(+)) cells was higher in the dermis of symptomatic than in asymptomatic dogs (p = 0.0368). A positive correlation between parasite density and macrophages density (p = 0.031), CD4(+) T-cells (p = 0.015), and CD8(+) T-cells (p = 0.023) was observed. Furthermore, a positive correlation between density of iNOS+ cells and CD3(+) T-cells (p = 0.005), CD4(+) T-cells (p = 0.001), and CD8+ T-cells (p = 0.0001) was also found. The results showed the existence of a non-specific chronic inflammatory infiltrate in the dermis of dogs affected by visceral leishmaniasis, characterized by the presence of activated macrophages and T-lymphocytes, associated to cutaneous parasitism, independent of clinical status. |
id |
UNSP_7377d7885c5ffe7b2055b14155439419 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/161780 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASISCanine visceral leishmaniasisCutaneous lesionHistopathologyCellular immune responseThirty-eight dogs naturally affected by visceral leishmaniasis were recruited in Aracatuba, Sao Paulo State, Brazil - an endemic area for visceral leishmaniasis. The animals were distributed into one of two groups, according to their clinical and laboratory features, as either symptomatic or asymptomatic dogs. Correlations between clinical features and inflammatory patterns, cellular immune responses, and parasitism in the macroscopically uninjured skin of the ear were investigated. Histological skin patterns were similar in both groups, and were generally characterized by a mild to intense inflammatory infiltrate in the dermis, mainly consisting of mononuclear cells. There was no difference in the number of parasites in the skin (amastigotes/mm(2)) between the two groups. Concerning the characterization of the cellular immune response, the number of positive inducible nitric oxide synthase (iNOS(+)) cells was higher in the dermis of symptomatic than in asymptomatic dogs (p = 0.0368). A positive correlation between parasite density and macrophages density (p = 0.031), CD4(+) T-cells (p = 0.015), and CD8(+) T-cells (p = 0.023) was observed. Furthermore, a positive correlation between density of iNOS+ cells and CD3(+) T-cells (p = 0.005), CD4(+) T-cells (p = 0.001), and CD8+ T-cells (p = 0.0001) was also found. The results showed the existence of a non-specific chronic inflammatory infiltrate in the dermis of dogs affected by visceral leishmaniasis, characterized by the presence of activated macrophages and T-lymphocytes, associated to cutaneous parasitism, independent of clinical status.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)LIM50 HC-FMUSPUniv Sao Paulo, Dept Clin, Sch Vet Med & Anim Sci, Sao Paulo, SP, BrazilUniv Sao Paulo, Dept Pathol LIM 50, Med School, Sao Paulo, SP, BrazilUniv Sao Paulo, Dept Pathol, Sch Vet Med & Anim Sci, Sao Paulo, SP, BrazilSao Paulo State Univ, Dept Anim Hlth & Prod, Sch Vet Medicine, Aracatuba, SP, BrazilSao Paulo State Univ, Dept Anim Hlth & Prod, Sch Vet Medicine, Aracatuba, SP, BrazilFAPESP: 2004/07965-2FAPESP: 2012/50285-9CNPq: 476479/2012-6Inst Medicina Tropical Sao PauloUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Rossi, Claudio NazaretianTomokane, Thaise YumieSilva Batista, Luis Fabio daMarcondes, Mary [UNESP]Larsson, Carlos EduardoLaurenti, Marcia Dalastra2018-11-26T16:48:35Z2018-11-26T16:48:35Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1590/S1678-9946201658048Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 58, 8 p., 2016.0036-4665http://hdl.handle.net/11449/16178010.1590/S1678-9946201658048S0036-46652016005000235WOS:000380680200036S0036-46652016005000235.pdf1817946671090010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Do Instituto De Medicina Tropical De Sao Paulo0,669info:eu-repo/semantics/openAccess2023-12-29T06:15:50Zoai:repositorio.unesp.br:11449/161780Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-29T06:15:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS |
title |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS |
spellingShingle |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS Rossi, Claudio Nazaretian Canine visceral leishmaniasis Cutaneous lesion Histopathology Cellular immune response |
title_short |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS |
title_full |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS |
title_fullStr |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS |
title_full_unstemmed |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS |
title_sort |
In situ CUTANEOUS CELLULAR IMMUNE RESPONSE IN DOGS NATURALLY AFFECTED BY VISCERAL LEISHMANIASIS |
author |
Rossi, Claudio Nazaretian |
author_facet |
Rossi, Claudio Nazaretian Tomokane, Thaise Yumie Silva Batista, Luis Fabio da Marcondes, Mary [UNESP] Larsson, Carlos Eduardo Laurenti, Marcia Dalastra |
author_role |
author |
author2 |
Tomokane, Thaise Yumie Silva Batista, Luis Fabio da Marcondes, Mary [UNESP] Larsson, Carlos Eduardo Laurenti, Marcia Dalastra |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rossi, Claudio Nazaretian Tomokane, Thaise Yumie Silva Batista, Luis Fabio da Marcondes, Mary [UNESP] Larsson, Carlos Eduardo Laurenti, Marcia Dalastra |
dc.subject.por.fl_str_mv |
Canine visceral leishmaniasis Cutaneous lesion Histopathology Cellular immune response |
topic |
Canine visceral leishmaniasis Cutaneous lesion Histopathology Cellular immune response |
description |
Thirty-eight dogs naturally affected by visceral leishmaniasis were recruited in Aracatuba, Sao Paulo State, Brazil - an endemic area for visceral leishmaniasis. The animals were distributed into one of two groups, according to their clinical and laboratory features, as either symptomatic or asymptomatic dogs. Correlations between clinical features and inflammatory patterns, cellular immune responses, and parasitism in the macroscopically uninjured skin of the ear were investigated. Histological skin patterns were similar in both groups, and were generally characterized by a mild to intense inflammatory infiltrate in the dermis, mainly consisting of mononuclear cells. There was no difference in the number of parasites in the skin (amastigotes/mm(2)) between the two groups. Concerning the characterization of the cellular immune response, the number of positive inducible nitric oxide synthase (iNOS(+)) cells was higher in the dermis of symptomatic than in asymptomatic dogs (p = 0.0368). A positive correlation between parasite density and macrophages density (p = 0.031), CD4(+) T-cells (p = 0.015), and CD8(+) T-cells (p = 0.023) was observed. Furthermore, a positive correlation between density of iNOS+ cells and CD3(+) T-cells (p = 0.005), CD4(+) T-cells (p = 0.001), and CD8+ T-cells (p = 0.0001) was also found. The results showed the existence of a non-specific chronic inflammatory infiltrate in the dermis of dogs affected by visceral leishmaniasis, characterized by the presence of activated macrophages and T-lymphocytes, associated to cutaneous parasitism, independent of clinical status. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-11-26T16:48:35Z 2018-11-26T16:48:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-9946201658048 Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 58, 8 p., 2016. 0036-4665 http://hdl.handle.net/11449/161780 10.1590/S1678-9946201658048 S0036-46652016005000235 WOS:000380680200036 S0036-46652016005000235.pdf 1817946671090010 |
url |
http://dx.doi.org/10.1590/S1678-9946201658048 http://hdl.handle.net/11449/161780 |
identifier_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 58, 8 p., 2016. 0036-4665 10.1590/S1678-9946201658048 S0036-46652016005000235 WOS:000380680200036 S0036-46652016005000235.pdf 1817946671090010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo 0,669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790139817132032 |