Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/cctc.202000320 http://hdl.handle.net/11449/200546 |
Resumo: | The crude naringinase from Penicillium decumbens and a purified naringinase with high α-L-rhamnosidase activity could be covalently immobilized on two-dimensional zeolite ITQ-2 after surface modification with glutaraldehyde. The influence of pH and temperature on the enzyme activity (in free and immobilized forms) as well as the thermal stability were determined using the specific substrate: p-nitrophenyl-alpha-L-rhamnopyranoside (Rha-pNP). The crude and purified naringinase supported on ITQ-2 were applied in the hydrolysis of naringin, giving the flavonoids naringenin and prunin respectively with a conversion '90 % and excellent selectivity. The supported enzymes showed long term stability, being possible to perform up to 25 consecutive cycles without loss of activity, showing its high potential to produce the valuable citrus flavonoids prunin and naringenin. We have also succeeded in the application of the immobilized crude naringinase on ITQ-2 for debittering grapefruit juices in a continuous process that was maintained operating for 300 h, with excellent results. |
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Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of JuicesITQ-2 zeolitejuice citrus debitteringnaringeninnaringinpruninsupported naringinaseThe crude naringinase from Penicillium decumbens and a purified naringinase with high α-L-rhamnosidase activity could be covalently immobilized on two-dimensional zeolite ITQ-2 after surface modification with glutaraldehyde. The influence of pH and temperature on the enzyme activity (in free and immobilized forms) as well as the thermal stability were determined using the specific substrate: p-nitrophenyl-alpha-L-rhamnopyranoside (Rha-pNP). The crude and purified naringinase supported on ITQ-2 were applied in the hydrolysis of naringin, giving the flavonoids naringenin and prunin respectively with a conversion '90 % and excellent selectivity. The supported enzymes showed long term stability, being possible to perform up to 25 consecutive cycles without loss of activity, showing its high potential to produce the valuable citrus flavonoids prunin and naringenin. We have also succeeded in the application of the immobilized crude naringinase on ITQ-2 for debittering grapefruit juices in a continuous process that was maintained operating for 300 h, with excellent results.Universitat Politècnica de València Institute of Chemical Technology (ITQ) – Valencia, Avenida Los Naranjos s/nSchool of Nutrition and Dietetic University of AntioquiaDepartment of Food and Nutrition Faculdade de Ciências Farmacêuticas UNESP – Univ EstadualDepartment of Biocatalysis Institute of Catalysis (ICP-CSIC), Marie Curie 2 Cantoblanco CampusState key Laboratory of Inorganic Synthesis & Preparative Chemistry Jilin University, 2699 Qianjin StreetDepartment of Food and Nutrition Faculdade de Ciências Farmacêuticas UNESP – Univ EstadualInstitute of Chemical Technology (ITQ) – ValenciaUniversity of AntioquiaUniversidade Estadual Paulista (Unesp)Jilin UniversityCarceller, Jose MiguelMartínez Galán, Julián PaulMonti, Rubens [UNESP]Bassan, Juliana Cristina [UNESP]Filice, Marco [UNESP]Yu, JihongCliment, María J.Iborra, SaraCorma, Avelino2020-12-12T02:09:27Z2020-12-12T02:09:27Z2020-09-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4502-4511http://dx.doi.org/10.1002/cctc.202000320ChemCatChem, v. 12, n. 18, p. 4502-4511, 2020.1867-38991867-3880http://hdl.handle.net/11449/20054610.1002/cctc.2020003202-s2.0-85085751464Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemCatCheminfo:eu-repo/semantics/openAccess2021-10-23T14:40:28Zoai:repositorio.unesp.br:11449/200546Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:40:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices |
title |
Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices |
spellingShingle |
Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices Carceller, Jose Miguel ITQ-2 zeolite juice citrus debittering naringenin naringin prunin supported naringinase |
title_short |
Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices |
title_full |
Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices |
title_fullStr |
Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices |
title_full_unstemmed |
Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices |
title_sort |
Covalent Immobilization of Naringinase over Two-Dimensional 2D Zeolites and its Applications in a Continuous Process to Produce Citrus Flavonoids and for Debittering of Juices |
author |
Carceller, Jose Miguel |
author_facet |
Carceller, Jose Miguel Martínez Galán, Julián Paul Monti, Rubens [UNESP] Bassan, Juliana Cristina [UNESP] Filice, Marco [UNESP] Yu, Jihong Climent, María J. Iborra, Sara Corma, Avelino |
author_role |
author |
author2 |
Martínez Galán, Julián Paul Monti, Rubens [UNESP] Bassan, Juliana Cristina [UNESP] Filice, Marco [UNESP] Yu, Jihong Climent, María J. Iborra, Sara Corma, Avelino |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Institute of Chemical Technology (ITQ) – Valencia University of Antioquia Universidade Estadual Paulista (Unesp) Jilin University |
dc.contributor.author.fl_str_mv |
Carceller, Jose Miguel Martínez Galán, Julián Paul Monti, Rubens [UNESP] Bassan, Juliana Cristina [UNESP] Filice, Marco [UNESP] Yu, Jihong Climent, María J. Iborra, Sara Corma, Avelino |
dc.subject.por.fl_str_mv |
ITQ-2 zeolite juice citrus debittering naringenin naringin prunin supported naringinase |
topic |
ITQ-2 zeolite juice citrus debittering naringenin naringin prunin supported naringinase |
description |
The crude naringinase from Penicillium decumbens and a purified naringinase with high α-L-rhamnosidase activity could be covalently immobilized on two-dimensional zeolite ITQ-2 after surface modification with glutaraldehyde. The influence of pH and temperature on the enzyme activity (in free and immobilized forms) as well as the thermal stability were determined using the specific substrate: p-nitrophenyl-alpha-L-rhamnopyranoside (Rha-pNP). The crude and purified naringinase supported on ITQ-2 were applied in the hydrolysis of naringin, giving the flavonoids naringenin and prunin respectively with a conversion '90 % and excellent selectivity. The supported enzymes showed long term stability, being possible to perform up to 25 consecutive cycles without loss of activity, showing its high potential to produce the valuable citrus flavonoids prunin and naringenin. We have also succeeded in the application of the immobilized crude naringinase on ITQ-2 for debittering grapefruit juices in a continuous process that was maintained operating for 300 h, with excellent results. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:09:27Z 2020-12-12T02:09:27Z 2020-09-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/cctc.202000320 ChemCatChem, v. 12, n. 18, p. 4502-4511, 2020. 1867-3899 1867-3880 http://hdl.handle.net/11449/200546 10.1002/cctc.202000320 2-s2.0-85085751464 |
url |
http://dx.doi.org/10.1002/cctc.202000320 http://hdl.handle.net/11449/200546 |
identifier_str_mv |
ChemCatChem, v. 12, n. 18, p. 4502-4511, 2020. 1867-3899 1867-3880 10.1002/cctc.202000320 2-s2.0-85085751464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ChemCatChem |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4502-4511 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797789585147691008 |