Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-7757-2016-0517 http://hdl.handle.net/11449/163424 |
Resumo: | Periodontal research involves the use of animal models to better understand the biological processes of periodontal diseases and the potential of new or existing therapies. Currently, ligature-induced periodontitis in rats is the main model used in periodontal research, in this model, alveolar bone loss (ABL) is the main parameter evaluated by radiographic, morphometric, and histological techniques. Interestingly, although these methodologies are widely used, it is not totally clarified neither the kinetics of ABL over the induction time nor the agreement degree (repeatability and reproducibility) of these techniques. Objective: To characterize ABL kinetics at 0, 3, 7, 15, 30, and 60 days after ABL induction by ligature and to evaluate the intra- (repeatability) and inter-examiner(reproducibility) agreement and the correlation among the radiographic, morphometric, and histological methodologies. Material and Methods: 60 male Wistar rats with induced ABL were randomly divided into 6 experimental groups (n=10 aninnals/group). After 0, 3, 7, 15, 30, and 60 days, the animals were euthanized and their hennimandibles were removed for ABL determination using radiographic, morphometric and histological techniques. Results: Radiographic and morphometric/linear techniques allowed the detection of statistically significant ABL on the third day, while histological and morphometric/area techniques could only detect ABL after the seventh day (ANOVA/Tukey, p<0.05). After the fifteenth day, except for histological analysis, the ABL was stabilized. Concerning the agreement of the methodologies, Bland Altman's test (intra and inter-examiner evaluations) showed no difference among the measurements (p>0.05). In addition, high correlations (Pearson's test, r2>0.9, p<0.05) were observed. Conclusion: The results indicated that the minimum time for ABL induction could vary from 3 to 7 days, according to the chosen analysis methodology. Agreement and correlation data support the comparison of results between studies with same induction time. |
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Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal modelExperimental animal modelsPeriodontitisAlveolar bone lossPeriodontal research involves the use of animal models to better understand the biological processes of periodontal diseases and the potential of new or existing therapies. Currently, ligature-induced periodontitis in rats is the main model used in periodontal research, in this model, alveolar bone loss (ABL) is the main parameter evaluated by radiographic, morphometric, and histological techniques. Interestingly, although these methodologies are widely used, it is not totally clarified neither the kinetics of ABL over the induction time nor the agreement degree (repeatability and reproducibility) of these techniques. Objective: To characterize ABL kinetics at 0, 3, 7, 15, 30, and 60 days after ABL induction by ligature and to evaluate the intra- (repeatability) and inter-examiner(reproducibility) agreement and the correlation among the radiographic, morphometric, and histological methodologies. Material and Methods: 60 male Wistar rats with induced ABL were randomly divided into 6 experimental groups (n=10 aninnals/group). After 0, 3, 7, 15, 30, and 60 days, the animals were euthanized and their hennimandibles were removed for ABL determination using radiographic, morphometric and histological techniques. Results: Radiographic and morphometric/linear techniques allowed the detection of statistically significant ABL on the third day, while histological and morphometric/area techniques could only detect ABL after the seventh day (ANOVA/Tukey, p<0.05). After the fifteenth day, except for histological analysis, the ABL was stabilized. Concerning the agreement of the methodologies, Bland Altman's test (intra and inter-examiner evaluations) showed no difference among the measurements (p>0.05). In addition, high correlations (Pearson's test, r2>0.9, p<0.05) were observed. Conclusion: The results indicated that the minimum time for ABL induction could vary from 3 to 7 days, according to the chosen analysis methodology. Agreement and correlation data support the comparison of results between studies with same induction time.Univ Estadual Ponta Grossa, Dept Odontol, Av Gen Cados Cavalcanti 4748, BR-84030900 Ponta Grossa, PR, BrazilUniv Estadual Paulista, Dept Biociencias & Diagnost Oral, Sao Jose Dos Campos, SP, BrazilUniv Estadual Ponta Grossa, Programa Posgrad Ciencias Biomed, Ponta Grossa, PR, BrazilUniv Estadual Ponta Grossa, Programa Grad Enfermagem, Ponta Grossa, PR, BrazilUniv Estadual Ponta Grossa, Dept Biol, Ponta Grossa, PR, BrazilUniv Estadual Paulista, Dept Biociencias & Diagnost Oral, Sao Jose Dos Campos, SP, BrazilUniv Sao Paulo Fac Odontologia BauruUniversidade Estadual de Ponta Grossa (UEPG)Universidade Estadual Paulista (Unesp)Vargas-Sanchez, Paula KatherineMoro, Marcella GoetzSantos, Fabio Andre dosAnbinder, Ana Lia [UNESP]Kreich, ElianeMoraes, Renata Mendonca [UNESP]Padilha, LauryellenKusiak, CarolineScomparin, Dionizia XavierNobre Franco, Gilson Cesar2018-11-26T17:41:58Z2018-11-26T17:41:58Z2017-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article490-497application/pdfhttp://dx.doi.org/10.1590/1678-7757-2016-0517Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 25, n. 5, p. 490-497, 2017.1678-7757http://hdl.handle.net/11449/16342410.1590/1678-7757-2016-0517S1678-77572017000500490WOS:000413737100004S1678-77572017000500490.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Applied Oral Science0,645info:eu-repo/semantics/openAccess2023-12-20T06:17:47Zoai:repositorio.unesp.br:11449/163424Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-20T06:17:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model |
title |
Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model |
spellingShingle |
Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model Vargas-Sanchez, Paula Katherine Experimental animal models Periodontitis Alveolar bone loss |
title_short |
Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model |
title_full |
Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model |
title_fullStr |
Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model |
title_full_unstemmed |
Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model |
title_sort |
Agreement, correlation, and kinetics of the alveolar bone-loss measurement methodologies in a ligature-induced periodontitis animal model |
author |
Vargas-Sanchez, Paula Katherine |
author_facet |
Vargas-Sanchez, Paula Katherine Moro, Marcella Goetz Santos, Fabio Andre dos Anbinder, Ana Lia [UNESP] Kreich, Eliane Moraes, Renata Mendonca [UNESP] Padilha, Lauryellen Kusiak, Caroline Scomparin, Dionizia Xavier Nobre Franco, Gilson Cesar |
author_role |
author |
author2 |
Moro, Marcella Goetz Santos, Fabio Andre dos Anbinder, Ana Lia [UNESP] Kreich, Eliane Moraes, Renata Mendonca [UNESP] Padilha, Lauryellen Kusiak, Caroline Scomparin, Dionizia Xavier Nobre Franco, Gilson Cesar |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Ponta Grossa (UEPG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vargas-Sanchez, Paula Katherine Moro, Marcella Goetz Santos, Fabio Andre dos Anbinder, Ana Lia [UNESP] Kreich, Eliane Moraes, Renata Mendonca [UNESP] Padilha, Lauryellen Kusiak, Caroline Scomparin, Dionizia Xavier Nobre Franco, Gilson Cesar |
dc.subject.por.fl_str_mv |
Experimental animal models Periodontitis Alveolar bone loss |
topic |
Experimental animal models Periodontitis Alveolar bone loss |
description |
Periodontal research involves the use of animal models to better understand the biological processes of periodontal diseases and the potential of new or existing therapies. Currently, ligature-induced periodontitis in rats is the main model used in periodontal research, in this model, alveolar bone loss (ABL) is the main parameter evaluated by radiographic, morphometric, and histological techniques. Interestingly, although these methodologies are widely used, it is not totally clarified neither the kinetics of ABL over the induction time nor the agreement degree (repeatability and reproducibility) of these techniques. Objective: To characterize ABL kinetics at 0, 3, 7, 15, 30, and 60 days after ABL induction by ligature and to evaluate the intra- (repeatability) and inter-examiner(reproducibility) agreement and the correlation among the radiographic, morphometric, and histological methodologies. Material and Methods: 60 male Wistar rats with induced ABL were randomly divided into 6 experimental groups (n=10 aninnals/group). After 0, 3, 7, 15, 30, and 60 days, the animals were euthanized and their hennimandibles were removed for ABL determination using radiographic, morphometric and histological techniques. Results: Radiographic and morphometric/linear techniques allowed the detection of statistically significant ABL on the third day, while histological and morphometric/area techniques could only detect ABL after the seventh day (ANOVA/Tukey, p<0.05). After the fifteenth day, except for histological analysis, the ABL was stabilized. Concerning the agreement of the methodologies, Bland Altman's test (intra and inter-examiner evaluations) showed no difference among the measurements (p>0.05). In addition, high correlations (Pearson's test, r2>0.9, p<0.05) were observed. Conclusion: The results indicated that the minimum time for ABL induction could vary from 3 to 7 days, according to the chosen analysis methodology. Agreement and correlation data support the comparison of results between studies with same induction time. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 2018-11-26T17:41:58Z 2018-11-26T17:41:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-7757-2016-0517 Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 25, n. 5, p. 490-497, 2017. 1678-7757 http://hdl.handle.net/11449/163424 10.1590/1678-7757-2016-0517 S1678-77572017000500490 WOS:000413737100004 S1678-77572017000500490.pdf |
url |
http://dx.doi.org/10.1590/1678-7757-2016-0517 http://hdl.handle.net/11449/163424 |
identifier_str_mv |
Journal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 25, n. 5, p. 490-497, 2017. 1678-7757 10.1590/1678-7757-2016-0517 S1678-77572017000500490 WOS:000413737100004 S1678-77572017000500490.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Applied Oral Science 0,645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
490-497 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Sao Paulo Fac Odontologia Bauru |
publisher.none.fl_str_mv |
Univ Sao Paulo Fac Odontologia Bauru |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790053930369024 |