Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/nu14132567 http://hdl.handle.net/11449/241668 |
Resumo: | Diabetes associated with post-menopause is related to a worse condition of kidney dis-ease. Taking into consideration that this disorder may be regulated by estrogenic mediators, we evaluated the renal protective effect of isoflavone. We investigated the role of the PPARγ in the pathogenesis of the disease. For this study, we used diabetic female rats in a postmenopausal model through ovariectomy. The animals were treated with isoflavone or 17β-estradiol. A dosage was ad-ministered to bring on blood glycemia, and through immunohistochemistry, we evaluated the im-munoreactivity of PPARγ in the endometrium and renal tissue. We analyzed the immunoreactivity of renal injury molecule KIM-1 and the collagen and glycogen densities in the kidney. Through bioinformatics analysis, we observed PPARγ and COL1A1 gene expression under the influence of different glucose doses. In particular, we observed that isoflavone and 17β-estradiol regulate blood glycemia. Renal injury was inhibited by isoflavone, observed by a reduction in KIM-1, along with glycogen accumulation. These benefits of isoflavone may be associated with PPARγ overexpression in the kidneys and endometrium of diabetic ovariectomized rats. |
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Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γdiabetesisoflavonekidneypostmenopausalPPARγDiabetes associated with post-menopause is related to a worse condition of kidney dis-ease. Taking into consideration that this disorder may be regulated by estrogenic mediators, we evaluated the renal protective effect of isoflavone. We investigated the role of the PPARγ in the pathogenesis of the disease. For this study, we used diabetic female rats in a postmenopausal model through ovariectomy. The animals were treated with isoflavone or 17β-estradiol. A dosage was ad-ministered to bring on blood glycemia, and through immunohistochemistry, we evaluated the im-munoreactivity of PPARγ in the endometrium and renal tissue. We analyzed the immunoreactivity of renal injury molecule KIM-1 and the collagen and glycogen densities in the kidney. Through bioinformatics analysis, we observed PPARγ and COL1A1 gene expression under the influence of different glucose doses. In particular, we observed that isoflavone and 17β-estradiol regulate blood glycemia. Renal injury was inhibited by isoflavone, observed by a reduction in KIM-1, along with glycogen accumulation. These benefits of isoflavone may be associated with PPARγ overexpression in the kidneys and endometrium of diabetic ovariectomized rats.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Structural and Functional Biology Graduate Program Paulista School of Medicine Federal University of São Paulo (EPM/UNIFESP), 740 Edifício Lemos Torres—2° andar, Vila Clementino, SPDepartment of Gynecology Paulista School of Medicine Federal University of São Paulo (EPM/UNIFESP), SPBiosciences Graduate Program Institute of Biosciences Letters and Exact Sciences São Paulo State University (IBILCE/UNESP), SPDepartment of Obstetrics and Gynecology Medicine Faculty of University of São Paulo (FMUSP), SPQueen’s Cardiopulmonary Unit (QCPU) Queen’s UniversityDepartment of Medicine Nephrology Division Paulista School of Medicine Federal University of São Paulo (EPM/UNIFESP), SPInterdisciplinary Graduate Program in Health Sciences Cruzeiro do Sul University, SPBiosciences Graduate Program Institute of Biosciences Letters and Exact Sciences São Paulo State University (IBILCE/UNESP), SPFAPESP: 20/13405-2Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Queen’s UniversityCruzeiro do Sul UniversityFerraz Carbonel, Adriana Aparecidada Silva, Rafael André [UNESP]de Souza Ferreira, Luiz PhilipeVieira, Renata RamosDos Santos Simões, Ricardoda Silva Sasso, Gisela Rodriguesde Jesus Simões, ManuelSoares Junior, José MariaAzevedo Lima, Patrícia DanieleBorges, Fernanda Teixeira2023-03-01T21:15:52Z2023-03-01T21:15:52Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/nu14132567Nutrients, v. 14, n. 13, 2022.2072-6643http://hdl.handle.net/11449/24166810.3390/nu141325672-s2.0-85132244813Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNutrientsinfo:eu-repo/semantics/openAccess2023-03-01T21:15:52Zoai:repositorio.unesp.br:11449/241668Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T21:15:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ |
title |
Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ |
spellingShingle |
Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ Ferraz Carbonel, Adriana Aparecida diabetes isoflavone kidney postmenopausal PPARγ |
title_short |
Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ |
title_full |
Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ |
title_fullStr |
Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ |
title_full_unstemmed |
Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ |
title_sort |
Isoflavone Protects the Renal Tissue of Diabetic Ovariectomized Rats via PPAR-γ |
author |
Ferraz Carbonel, Adriana Aparecida |
author_facet |
Ferraz Carbonel, Adriana Aparecida da Silva, Rafael André [UNESP] de Souza Ferreira, Luiz Philipe Vieira, Renata Ramos Dos Santos Simões, Ricardo da Silva Sasso, Gisela Rodrigues de Jesus Simões, Manuel Soares Junior, José Maria Azevedo Lima, Patrícia Daniele Borges, Fernanda Teixeira |
author_role |
author |
author2 |
da Silva, Rafael André [UNESP] de Souza Ferreira, Luiz Philipe Vieira, Renata Ramos Dos Santos Simões, Ricardo da Silva Sasso, Gisela Rodrigues de Jesus Simões, Manuel Soares Junior, José Maria Azevedo Lima, Patrícia Daniele Borges, Fernanda Teixeira |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Queen’s University Cruzeiro do Sul University |
dc.contributor.author.fl_str_mv |
Ferraz Carbonel, Adriana Aparecida da Silva, Rafael André [UNESP] de Souza Ferreira, Luiz Philipe Vieira, Renata Ramos Dos Santos Simões, Ricardo da Silva Sasso, Gisela Rodrigues de Jesus Simões, Manuel Soares Junior, José Maria Azevedo Lima, Patrícia Daniele Borges, Fernanda Teixeira |
dc.subject.por.fl_str_mv |
diabetes isoflavone kidney postmenopausal PPARγ |
topic |
diabetes isoflavone kidney postmenopausal PPARγ |
description |
Diabetes associated with post-menopause is related to a worse condition of kidney dis-ease. Taking into consideration that this disorder may be regulated by estrogenic mediators, we evaluated the renal protective effect of isoflavone. We investigated the role of the PPARγ in the pathogenesis of the disease. For this study, we used diabetic female rats in a postmenopausal model through ovariectomy. The animals were treated with isoflavone or 17β-estradiol. A dosage was ad-ministered to bring on blood glycemia, and through immunohistochemistry, we evaluated the im-munoreactivity of PPARγ in the endometrium and renal tissue. We analyzed the immunoreactivity of renal injury molecule KIM-1 and the collagen and glycogen densities in the kidney. Through bioinformatics analysis, we observed PPARγ and COL1A1 gene expression under the influence of different glucose doses. In particular, we observed that isoflavone and 17β-estradiol regulate blood glycemia. Renal injury was inhibited by isoflavone, observed by a reduction in KIM-1, along with glycogen accumulation. These benefits of isoflavone may be associated with PPARγ overexpression in the kidneys and endometrium of diabetic ovariectomized rats. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 2023-03-01T21:15:52Z 2023-03-01T21:15:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/nu14132567 Nutrients, v. 14, n. 13, 2022. 2072-6643 http://hdl.handle.net/11449/241668 10.3390/nu14132567 2-s2.0-85132244813 |
url |
http://dx.doi.org/10.3390/nu14132567 http://hdl.handle.net/11449/241668 |
identifier_str_mv |
Nutrients, v. 14, n. 13, 2022. 2072-6643 10.3390/nu14132567 2-s2.0-85132244813 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nutrients |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790399220154368 |