Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572005000400012 http://hdl.handle.net/11449/68574 |
Resumo: | Herbicides inhibit enzymatic systems of plants. Acetolactate synthase (ALS, EC = 4.1.3.18) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, EC 2.5.1.19) are key enzymes for herbicide action. Hundreds of compounds inhibit ALS. This enzyme is highly variable, enabling the selective control of weeds in a number of crops. Glyphosate, the only commercial herbicide inhibiting EPSPS is widely used for non-selective control of weeds in many crops. Recently, transgenic crops resistant to glyphosate were developed and have been used by farmers. The aim of this study was the data mining of eucalypt expressed sequence tags (ESTs) in the FORESTs Genome Project database (https://forests.esalq.usp.br) related to these enzymes. Representative amino acid sequences from the NCBI database associated with ALS and EPSPS were blasted with ESTs from the FORESTs database using the tBLASTx option of the blast tool. The best blasting reads and clusters from FORESTs, represented as nucleotide sequences, were blasted back with the NCBI database to evaluate the level of similarity with available sequences from different species. One and seven clusters were identified as showing high similarity with EPSPS and ALS sequences from the literature, respectively. The alignment of EPSPS sequences allowed the identification of conserved regions that can be used to design specific primers for additional sequencings. Copyright by the Brazilian Society of Genetics. |
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Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesisALSEPSPSEucalyptusHerbicides3 phosphoshikimate 1 carboxyvinyltransferaseacetolactate synthasearomatic amino acidbranched chain amino acidglyphosateherbicideagricultural workeramino acid sequenceamino acid synthesiscontrolled studycropdata baseenzyme inhibitionexpressed sequence tagFORESTs Genome Project databaseGenBankgene clusterherbicide resistanceinformation processingnonhumannucleotide sequencetransgenic plantweed controlHerbicides inhibit enzymatic systems of plants. Acetolactate synthase (ALS, EC = 4.1.3.18) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, EC 2.5.1.19) are key enzymes for herbicide action. Hundreds of compounds inhibit ALS. This enzyme is highly variable, enabling the selective control of weeds in a number of crops. Glyphosate, the only commercial herbicide inhibiting EPSPS is widely used for non-selective control of weeds in many crops. Recently, transgenic crops resistant to glyphosate were developed and have been used by farmers. The aim of this study was the data mining of eucalypt expressed sequence tags (ESTs) in the FORESTs Genome Project database (https://forests.esalq.usp.br) related to these enzymes. Representative amino acid sequences from the NCBI database associated with ALS and EPSPS were blasted with ESTs from the FORESTs database using the tBLASTx option of the blast tool. The best blasting reads and clusters from FORESTs, represented as nucleotide sequences, were blasted back with the NCBI database to evaluate the level of similarity with available sequences from different species. One and seven clusters were identified as showing high similarity with EPSPS and ALS sequences from the literature, respectively. The alignment of EPSPS sequences allowed the identification of conserved regions that can be used to design specific primers for additional sequencings. Copyright by the Brazilian Society of Genetics.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias Laboratório de Motologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Laboratório de Xenobióticos, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Laboratório de Genética, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Melhoramento Vegetal, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Fitopatologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Solos, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Entomologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias Laboratório de Motologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Laboratório de Xenobióticos, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Instituto de Biociências Laboratório de Genética, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Melhoramento Vegetal, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Fitopatologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Solos, 18603-970 Botucatu, SPUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade Ciências Agrárias Laboratório de Entomologia, 18603-970 Botucatu, SPUniversidade Estadual Paulista (Unesp)Velini, Edivaldo Domingues [UNESP]Trindade, Maria Lúcia Bueno [UNESP]Alves, Elza [UNESP]Catâneo, Ana Catarina [UNESP]Marino, Celso Luis [UNESP]de Godoy Maia, Ivan [UNESP]Mori, Edson Seizo [UNESP]Furtado, Edson Luiz [UNESP]Guerrini, Iraê Amaral [UNESP]Wilcken, Carlos Frederico [UNESP]2014-05-27T11:21:43Z2014-05-27T11:21:43Z2005-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article575-581application/pdfhttp://dx.doi.org/10.1590/S1415-47572005000400012Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 575-581, 2005.1415-47571678-4685http://hdl.handle.net/11449/6857410.1590/S1415-47572005000400012S1415-47572005000400012WOS:0002043898000122-s2.0-308444711312-s2.0-30844471131.pdf864922209917616284590425674861090165348738208319985549344816170238459894858333950000-0003-0431-59420000-0002-6924-835X0000-0003-4524-954XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-04-30T15:57:15Zoai:repositorio.unesp.br:11449/68574Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T15:57:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis |
title |
Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis |
spellingShingle |
Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis Velini, Edivaldo Domingues [UNESP] ALS EPSPS Eucalyptus Herbicides 3 phosphoshikimate 1 carboxyvinyltransferase acetolactate synthase aromatic amino acid branched chain amino acid glyphosate herbicide agricultural worker amino acid sequence amino acid synthesis controlled study crop data base enzyme inhibition expressed sequence tag FORESTs Genome Project database GenBank gene cluster herbicide resistance information processing nonhuman nucleotide sequence transgenic plant weed control |
title_short |
Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis |
title_full |
Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis |
title_fullStr |
Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis |
title_full_unstemmed |
Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis |
title_sort |
Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis |
author |
Velini, Edivaldo Domingues [UNESP] |
author_facet |
Velini, Edivaldo Domingues [UNESP] Trindade, Maria Lúcia Bueno [UNESP] Alves, Elza [UNESP] Catâneo, Ana Catarina [UNESP] Marino, Celso Luis [UNESP] de Godoy Maia, Ivan [UNESP] Mori, Edson Seizo [UNESP] Furtado, Edson Luiz [UNESP] Guerrini, Iraê Amaral [UNESP] Wilcken, Carlos Frederico [UNESP] |
author_role |
author |
author2 |
Trindade, Maria Lúcia Bueno [UNESP] Alves, Elza [UNESP] Catâneo, Ana Catarina [UNESP] Marino, Celso Luis [UNESP] de Godoy Maia, Ivan [UNESP] Mori, Edson Seizo [UNESP] Furtado, Edson Luiz [UNESP] Guerrini, Iraê Amaral [UNESP] Wilcken, Carlos Frederico [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Velini, Edivaldo Domingues [UNESP] Trindade, Maria Lúcia Bueno [UNESP] Alves, Elza [UNESP] Catâneo, Ana Catarina [UNESP] Marino, Celso Luis [UNESP] de Godoy Maia, Ivan [UNESP] Mori, Edson Seizo [UNESP] Furtado, Edson Luiz [UNESP] Guerrini, Iraê Amaral [UNESP] Wilcken, Carlos Frederico [UNESP] |
dc.subject.por.fl_str_mv |
ALS EPSPS Eucalyptus Herbicides 3 phosphoshikimate 1 carboxyvinyltransferase acetolactate synthase aromatic amino acid branched chain amino acid glyphosate herbicide agricultural worker amino acid sequence amino acid synthesis controlled study crop data base enzyme inhibition expressed sequence tag FORESTs Genome Project database GenBank gene cluster herbicide resistance information processing nonhuman nucleotide sequence transgenic plant weed control |
topic |
ALS EPSPS Eucalyptus Herbicides 3 phosphoshikimate 1 carboxyvinyltransferase acetolactate synthase aromatic amino acid branched chain amino acid glyphosate herbicide agricultural worker amino acid sequence amino acid synthesis controlled study crop data base enzyme inhibition expressed sequence tag FORESTs Genome Project database GenBank gene cluster herbicide resistance information processing nonhuman nucleotide sequence transgenic plant weed control |
description |
Herbicides inhibit enzymatic systems of plants. Acetolactate synthase (ALS, EC = 4.1.3.18) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, EC 2.5.1.19) are key enzymes for herbicide action. Hundreds of compounds inhibit ALS. This enzyme is highly variable, enabling the selective control of weeds in a number of crops. Glyphosate, the only commercial herbicide inhibiting EPSPS is widely used for non-selective control of weeds in many crops. Recently, transgenic crops resistant to glyphosate were developed and have been used by farmers. The aim of this study was the data mining of eucalypt expressed sequence tags (ESTs) in the FORESTs Genome Project database (https://forests.esalq.usp.br) related to these enzymes. Representative amino acid sequences from the NCBI database associated with ALS and EPSPS were blasted with ESTs from the FORESTs database using the tBLASTx option of the blast tool. The best blasting reads and clusters from FORESTs, represented as nucleotide sequences, were blasted back with the NCBI database to evaluate the level of similarity with available sequences from different species. One and seven clusters were identified as showing high similarity with EPSPS and ALS sequences from the literature, respectively. The alignment of EPSPS sequences allowed the identification of conserved regions that can be used to design specific primers for additional sequencings. Copyright by the Brazilian Society of Genetics. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 2014-05-27T11:21:43Z 2014-05-27T11:21:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572005000400012 Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 575-581, 2005. 1415-4757 1678-4685 http://hdl.handle.net/11449/68574 10.1590/S1415-47572005000400012 S1415-47572005000400012 WOS:000204389800012 2-s2.0-30844471131 2-s2.0-30844471131.pdf 8649222099176162 8459042567486109 0165348738208319 9855493448161702 3845989485833395 0000-0003-0431-5942 0000-0002-6924-835X 0000-0003-4524-954X |
url |
http://dx.doi.org/10.1590/S1415-47572005000400012 http://hdl.handle.net/11449/68574 |
identifier_str_mv |
Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 575-581, 2005. 1415-4757 1678-4685 10.1590/S1415-47572005000400012 S1415-47572005000400012 WOS:000204389800012 2-s2.0-30844471131 2-s2.0-30844471131.pdf 8649222099176162 8459042567486109 0165348738208319 9855493448161702 3845989485833395 0000-0003-0431-5942 0000-0002-6924-835X 0000-0003-4524-954X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
575-581 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965152940916736 |