Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/113793 |
Resumo: | Lettuce (Lactuca sativa L.) belongs to the Asteraceae family and is one of the most consumed vegetables in Brazil. One of the major disease problems in this crop are plant viruses, especially the Lettuce mosaic virus (LMV) that cause losses to producers in various regions of the country, but also Lettuce mottle virus (LeMoV). As a form of control, the use of resistant varieties is the most effective way to overcome the damage caused by plant viruses. Most of the resistant varieties of lettuce makes the use of recessive genes, mostly involved in plant-virus interactions and characterized as eukaryotic translation initiation factor (eIFs). Thus, this study aimed to evaluate two lettuce genotypes (169501 and 169501C) for resistance to LMV and LeMoV. LMV and LeMoV were sap transmitted at different time of intervals. For LMV also transmissions with aphids were carried out. The two varieties tested were resistant to LMV in both forms of transmission, but showed symptoms when inoculated with LeMoV. In RT-PCR with specific primers, LMV was not detected but LeMoV yes, confirming the infection in the inoculated plants. The isolate of LMV used in the tests was not classified as belonging to subgroups Most ... |
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Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virusAlface - Resistencia a doenças e pragas - Aspectos geneticosAlface – VariedadesVirus de plantasPlantas - Efeito dos herbicidasDisease and pest resistance - Genetic aspectsLettuce (Lactuca sativa L.) belongs to the Asteraceae family and is one of the most consumed vegetables in Brazil. One of the major disease problems in this crop are plant viruses, especially the Lettuce mosaic virus (LMV) that cause losses to producers in various regions of the country, but also Lettuce mottle virus (LeMoV). As a form of control, the use of resistant varieties is the most effective way to overcome the damage caused by plant viruses. Most of the resistant varieties of lettuce makes the use of recessive genes, mostly involved in plant-virus interactions and characterized as eukaryotic translation initiation factor (eIFs). Thus, this study aimed to evaluate two lettuce genotypes (169501 and 169501C) for resistance to LMV and LeMoV. LMV and LeMoV were sap transmitted at different time of intervals. For LMV also transmissions with aphids were carried out. The two varieties tested were resistant to LMV in both forms of transmission, but showed symptoms when inoculated with LeMoV. In RT-PCR with specific primers, LMV was not detected but LeMoV yes, confirming the infection in the inoculated plants. The isolate of LMV used in the tests was not classified as belonging to subgroups Most ...A alface (Lactuca sativa L.), pertencente à família Asteraceae, é uma das hortaliças mais consumidas no Brasil. Um dos principais problemas fitossanitários para essa cultura são as fitoviroses, em especial o Lettuce mosaic virus (LMV), que causa elevados prejuízos aos produtores em diversas regiões do país, além do Lettuce mottle virus (LeMoV), que vem crescendo em importância nos últimos anos. Como forma de controle, o uso de variedades resistentes é maneira mais eficaz de contornar os danos causados por vírus. Na maioria das variedades resistentes de alface faz-se o uso de genes recessivos, em sua maioria envolvidos na interação planta-vírus, são os chamados codificadores de fatores de iniciação de tradução eucarióticos (eIFs). Diante disso, este trabalho teve como objetivo avaliar dois genótipos de alface (169501 e 169501C), quanto a resistência ao LMV e ao LeMoV. Foram realizadas transmissões via extrato vegetal nos dois genótipos de alface com LMV e LeMoV em diferentes intervalos de tempo. Para LMV também foram realizadas transmissões por afídeos. As duas variedades testadas se mostraram resistentes ao LMV nas duas formas de transmissão, mas apresentaram sintomas quando inoculadas com LeMoV. Em reações de RT-PCR com oligonucleotídeos específicos, foram comprovadas a ausência de LMV e a infecção por LeMoV nas plantas inoculadas. O isolado de LMV após análise do sequenciamento da porção N’ terminal da ...Universidade Estadual Paulista (Unesp)Sakate, Renate Krause [UNESP]Universidade Estadual Paulista (Unesp)Suzuki, Gerson Shinya [UNESP]2015-01-26T13:21:13Z2015-01-26T13:21:13Z2014-08-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisvi, 34 f. : il. color., gráfs, tabs.application/pdfSUZUKI, Gerson Shinya. Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus. 2014. vi, 34 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2014.http://hdl.handle.net/11449/113793000806750000806750.pdf33004064034P196598228556976850000-0001-7526-640XAlephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-12-22T06:20:08Zoai:repositorio.unesp.br:11449/113793Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-22T06:20:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus |
title |
Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus |
spellingShingle |
Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus Suzuki, Gerson Shinya [UNESP] Alface - Resistencia a doenças e pragas - Aspectos geneticos Alface – Variedades Virus de plantas Plantas - Efeito dos herbicidas Disease and pest resistance - Genetic aspects |
title_short |
Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus |
title_full |
Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus |
title_fullStr |
Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus |
title_full_unstemmed |
Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus |
title_sort |
Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus |
author |
Suzuki, Gerson Shinya [UNESP] |
author_facet |
Suzuki, Gerson Shinya [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Sakate, Renate Krause [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Suzuki, Gerson Shinya [UNESP] |
dc.subject.por.fl_str_mv |
Alface - Resistencia a doenças e pragas - Aspectos geneticos Alface – Variedades Virus de plantas Plantas - Efeito dos herbicidas Disease and pest resistance - Genetic aspects |
topic |
Alface - Resistencia a doenças e pragas - Aspectos geneticos Alface – Variedades Virus de plantas Plantas - Efeito dos herbicidas Disease and pest resistance - Genetic aspects |
description |
Lettuce (Lactuca sativa L.) belongs to the Asteraceae family and is one of the most consumed vegetables in Brazil. One of the major disease problems in this crop are plant viruses, especially the Lettuce mosaic virus (LMV) that cause losses to producers in various regions of the country, but also Lettuce mottle virus (LeMoV). As a form of control, the use of resistant varieties is the most effective way to overcome the damage caused by plant viruses. Most of the resistant varieties of lettuce makes the use of recessive genes, mostly involved in plant-virus interactions and characterized as eukaryotic translation initiation factor (eIFs). Thus, this study aimed to evaluate two lettuce genotypes (169501 and 169501C) for resistance to LMV and LeMoV. LMV and LeMoV were sap transmitted at different time of intervals. For LMV also transmissions with aphids were carried out. The two varieties tested were resistant to LMV in both forms of transmission, but showed symptoms when inoculated with LeMoV. In RT-PCR with specific primers, LMV was not detected but LeMoV yes, confirming the infection in the inoculated plants. The isolate of LMV used in the tests was not classified as belonging to subgroups Most ... |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-19 2015-01-26T13:21:13Z 2015-01-26T13:21:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SUZUKI, Gerson Shinya. Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus. 2014. vi, 34 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2014. http://hdl.handle.net/11449/113793 000806750 000806750.pdf 33004064034P1 9659822855697685 0000-0001-7526-640X |
identifier_str_mv |
SUZUKI, Gerson Shinya. Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus. 2014. vi, 34 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2014. 000806750 000806750.pdf 33004064034P1 9659822855697685 0000-0001-7526-640X |
url |
http://hdl.handle.net/11449/113793 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
vi, 34 f. : il. color., gráfs, tabs. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1792962143092473856 |