Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus

Detalhes bibliográficos
Autor(a) principal: Suzuki, Gerson Shinya [UNESP]
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/113793
Resumo: Lettuce (Lactuca sativa L.) belongs to the Asteraceae family and is one of the most consumed vegetables in Brazil. One of the major disease problems in this crop are plant viruses, especially the Lettuce mosaic virus (LMV) that cause losses to producers in various regions of the country, but also Lettuce mottle virus (LeMoV). As a form of control, the use of resistant varieties is the most effective way to overcome the damage caused by plant viruses. Most of the resistant varieties of lettuce makes the use of recessive genes, mostly involved in plant-virus interactions and characterized as eukaryotic translation initiation factor (eIFs). Thus, this study aimed to evaluate two lettuce genotypes (169501 and 169501C) for resistance to LMV and LeMoV. LMV and LeMoV were sap transmitted at different time of intervals. For LMV also transmissions with aphids were carried out. The two varieties tested were resistant to LMV in both forms of transmission, but showed symptoms when inoculated with LeMoV. In RT-PCR with specific primers, LMV was not detected but LeMoV yes, confirming the infection in the inoculated plants. The isolate of LMV used in the tests was not classified as belonging to subgroups Most ...
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spelling Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virusAlface - Resistencia a doenças e pragas - Aspectos geneticosAlface – VariedadesVirus de plantasPlantas - Efeito dos herbicidasDisease and pest resistance - Genetic aspectsLettuce (Lactuca sativa L.) belongs to the Asteraceae family and is one of the most consumed vegetables in Brazil. One of the major disease problems in this crop are plant viruses, especially the Lettuce mosaic virus (LMV) that cause losses to producers in various regions of the country, but also Lettuce mottle virus (LeMoV). As a form of control, the use of resistant varieties is the most effective way to overcome the damage caused by plant viruses. Most of the resistant varieties of lettuce makes the use of recessive genes, mostly involved in plant-virus interactions and characterized as eukaryotic translation initiation factor (eIFs). Thus, this study aimed to evaluate two lettuce genotypes (169501 and 169501C) for resistance to LMV and LeMoV. LMV and LeMoV were sap transmitted at different time of intervals. For LMV also transmissions with aphids were carried out. The two varieties tested were resistant to LMV in both forms of transmission, but showed symptoms when inoculated with LeMoV. In RT-PCR with specific primers, LMV was not detected but LeMoV yes, confirming the infection in the inoculated plants. The isolate of LMV used in the tests was not classified as belonging to subgroups Most ...A alface (Lactuca sativa L.), pertencente à família Asteraceae, é uma das hortaliças mais consumidas no Brasil. Um dos principais problemas fitossanitários para essa cultura são as fitoviroses, em especial o Lettuce mosaic virus (LMV), que causa elevados prejuízos aos produtores em diversas regiões do país, além do Lettuce mottle virus (LeMoV), que vem crescendo em importância nos últimos anos. Como forma de controle, o uso de variedades resistentes é maneira mais eficaz de contornar os danos causados por vírus. Na maioria das variedades resistentes de alface faz-se o uso de genes recessivos, em sua maioria envolvidos na interação planta-vírus, são os chamados codificadores de fatores de iniciação de tradução eucarióticos (eIFs). Diante disso, este trabalho teve como objetivo avaliar dois genótipos de alface (169501 e 169501C), quanto a resistência ao LMV e ao LeMoV. Foram realizadas transmissões via extrato vegetal nos dois genótipos de alface com LMV e LeMoV em diferentes intervalos de tempo. Para LMV também foram realizadas transmissões por afídeos. As duas variedades testadas se mostraram resistentes ao LMV nas duas formas de transmissão, mas apresentaram sintomas quando inoculadas com LeMoV. Em reações de RT-PCR com oligonucleotídeos específicos, foram comprovadas a ausência de LMV e a infecção por LeMoV nas plantas inoculadas. O isolado de LMV após análise do sequenciamento da porção N’ terminal da ...Universidade Estadual Paulista (Unesp)Sakate, Renate Krause [UNESP]Universidade Estadual Paulista (Unesp)Suzuki, Gerson Shinya [UNESP]2015-01-26T13:21:13Z2015-01-26T13:21:13Z2014-08-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisvi, 34 f. : il. color., gráfs, tabs.application/pdfSUZUKI, Gerson Shinya. Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus. 2014. vi, 34 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2014.http://hdl.handle.net/11449/113793000806750000806750.pdf33004064034P196598228556976850000-0001-7526-640XAlephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-12-22T06:20:08Zoai:repositorio.unesp.br:11449/113793Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-22T06:20:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
title Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
spellingShingle Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
Suzuki, Gerson Shinya [UNESP]
Alface - Resistencia a doenças e pragas - Aspectos geneticos
Alface – Variedades
Virus de plantas
Plantas - Efeito dos herbicidas
Disease and pest resistance - Genetic aspects
title_short Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
title_full Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
title_fullStr Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
title_full_unstemmed Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
title_sort Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus
author Suzuki, Gerson Shinya [UNESP]
author_facet Suzuki, Gerson Shinya [UNESP]
author_role author
dc.contributor.none.fl_str_mv Sakate, Renate Krause [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Suzuki, Gerson Shinya [UNESP]
dc.subject.por.fl_str_mv Alface - Resistencia a doenças e pragas - Aspectos geneticos
Alface – Variedades
Virus de plantas
Plantas - Efeito dos herbicidas
Disease and pest resistance - Genetic aspects
topic Alface - Resistencia a doenças e pragas - Aspectos geneticos
Alface – Variedades
Virus de plantas
Plantas - Efeito dos herbicidas
Disease and pest resistance - Genetic aspects
description Lettuce (Lactuca sativa L.) belongs to the Asteraceae family and is one of the most consumed vegetables in Brazil. One of the major disease problems in this crop are plant viruses, especially the Lettuce mosaic virus (LMV) that cause losses to producers in various regions of the country, but also Lettuce mottle virus (LeMoV). As a form of control, the use of resistant varieties is the most effective way to overcome the damage caused by plant viruses. Most of the resistant varieties of lettuce makes the use of recessive genes, mostly involved in plant-virus interactions and characterized as eukaryotic translation initiation factor (eIFs). Thus, this study aimed to evaluate two lettuce genotypes (169501 and 169501C) for resistance to LMV and LeMoV. LMV and LeMoV were sap transmitted at different time of intervals. For LMV also transmissions with aphids were carried out. The two varieties tested were resistant to LMV in both forms of transmission, but showed symptoms when inoculated with LeMoV. In RT-PCR with specific primers, LMV was not detected but LeMoV yes, confirming the infection in the inoculated plants. The isolate of LMV used in the tests was not classified as belonging to subgroups Most ...
publishDate 2014
dc.date.none.fl_str_mv 2014-08-19
2015-01-26T13:21:13Z
2015-01-26T13:21:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SUZUKI, Gerson Shinya. Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus. 2014. vi, 34 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2014.
http://hdl.handle.net/11449/113793
000806750
000806750.pdf
33004064034P1
9659822855697685
0000-0001-7526-640X
identifier_str_mv SUZUKI, Gerson Shinya. Comportamento de genótipos de alface com o alelo mo10 ao Lettuce mosaic virus e Lettuce mottle virus. 2014. vi, 34 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agronômicas de Botucatu, 2014.
000806750
000806750.pdf
33004064034P1
9659822855697685
0000-0001-7526-640X
url http://hdl.handle.net/11449/113793
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv vi, 34 f. : il. color., gráfs, tabs.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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