The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts

Detalhes bibliográficos
Autor(a) principal: Olímpio, Regiane Marques Castro [UNESP]
Data de Publicação: 2019
Outros Autores: Moretto, Fernanda Cristina Fontes [UNESP], De Sibio, Maria Teresa [UNESP], de Oliveira, Miriane [UNESP], Mathias, Lucas Solla [UNESP], Gonçalves, Bianca Mariani [UNESP], Deprá, Igor Carvalho [UNESP], Tilli, Helena Paim. [UNESP], Rodrigues, Bruna Moretto [UNESP], Saraiva, Patrícia Pinto [UNESP], Maria, Durvanei Augusto, Nogueira, Célia Regina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.lfs.2019.116556
http://hdl.handle.net/11449/189257
Resumo: Triiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T3. E2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess.
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spelling The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblastsEstrogenOsteoblastRANKL and OPGStem cellsTriiodothyronineTriiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T3. E2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Internal Medicine Botucatu Medical School Univ Estadual Paulista - UNESPLaboratory Biochemistry and Biophysics Butantan Institute, 1500, Avenue Vital BrazilDepartment of Internal Medicine Botucatu Medical School Univ Estadual Paulista - UNESPFAPESP: 2014/16406-9Universidade Estadual Paulista (Unesp)Butantan InstituteOlímpio, Regiane Marques Castro [UNESP]Moretto, Fernanda Cristina Fontes [UNESP]De Sibio, Maria Teresa [UNESP]de Oliveira, Miriane [UNESP]Mathias, Lucas Solla [UNESP]Gonçalves, Bianca Mariani [UNESP]Deprá, Igor Carvalho [UNESP]Tilli, Helena Paim. [UNESP]Rodrigues, Bruna Moretto [UNESP]Saraiva, Patrícia Pinto [UNESP]Maria, Durvanei AugustoNogueira, Célia Regina [UNESP]2019-10-06T16:34:57Z2019-10-06T16:34:57Z2019-08-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.lfs.2019.116556Life Sciences, v. 231.1879-06310024-3205http://hdl.handle.net/11449/18925710.1016/j.lfs.2019.1165562-s2.0-85067341769Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2024-08-14T17:22:13Zoai:repositorio.unesp.br:11449/189257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
title The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
spellingShingle The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
Olímpio, Regiane Marques Castro [UNESP]
Estrogen
Osteoblast
RANKL and OPG
Stem cells
Triiodothyronine
title_short The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
title_full The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
title_fullStr The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
title_full_unstemmed The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
title_sort The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
author Olímpio, Regiane Marques Castro [UNESP]
author_facet Olímpio, Regiane Marques Castro [UNESP]
Moretto, Fernanda Cristina Fontes [UNESP]
De Sibio, Maria Teresa [UNESP]
de Oliveira, Miriane [UNESP]
Mathias, Lucas Solla [UNESP]
Gonçalves, Bianca Mariani [UNESP]
Deprá, Igor Carvalho [UNESP]
Tilli, Helena Paim. [UNESP]
Rodrigues, Bruna Moretto [UNESP]
Saraiva, Patrícia Pinto [UNESP]
Maria, Durvanei Augusto
Nogueira, Célia Regina [UNESP]
author_role author
author2 Moretto, Fernanda Cristina Fontes [UNESP]
De Sibio, Maria Teresa [UNESP]
de Oliveira, Miriane [UNESP]
Mathias, Lucas Solla [UNESP]
Gonçalves, Bianca Mariani [UNESP]
Deprá, Igor Carvalho [UNESP]
Tilli, Helena Paim. [UNESP]
Rodrigues, Bruna Moretto [UNESP]
Saraiva, Patrícia Pinto [UNESP]
Maria, Durvanei Augusto
Nogueira, Célia Regina [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Butantan Institute
dc.contributor.author.fl_str_mv Olímpio, Regiane Marques Castro [UNESP]
Moretto, Fernanda Cristina Fontes [UNESP]
De Sibio, Maria Teresa [UNESP]
de Oliveira, Miriane [UNESP]
Mathias, Lucas Solla [UNESP]
Gonçalves, Bianca Mariani [UNESP]
Deprá, Igor Carvalho [UNESP]
Tilli, Helena Paim. [UNESP]
Rodrigues, Bruna Moretto [UNESP]
Saraiva, Patrícia Pinto [UNESP]
Maria, Durvanei Augusto
Nogueira, Célia Regina [UNESP]
dc.subject.por.fl_str_mv Estrogen
Osteoblast
RANKL and OPG
Stem cells
Triiodothyronine
topic Estrogen
Osteoblast
RANKL and OPG
Stem cells
Triiodothyronine
description Triiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T3. E2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:34:57Z
2019-10-06T16:34:57Z
2019-08-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.lfs.2019.116556
Life Sciences, v. 231.
1879-0631
0024-3205
http://hdl.handle.net/11449/189257
10.1016/j.lfs.2019.116556
2-s2.0-85067341769
url http://dx.doi.org/10.1016/j.lfs.2019.116556
http://hdl.handle.net/11449/189257
identifier_str_mv Life Sciences, v. 231.
1879-0631
0024-3205
10.1016/j.lfs.2019.116556
2-s2.0-85067341769
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Life Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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