The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.lfs.2019.116556 http://hdl.handle.net/11449/189257 |
Resumo: | Triiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T3. E2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess. |
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The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblastsEstrogenOsteoblastRANKL and OPGStem cellsTriiodothyronineTriiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T3. E2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Internal Medicine Botucatu Medical School Univ Estadual Paulista - UNESPLaboratory Biochemistry and Biophysics Butantan Institute, 1500, Avenue Vital BrazilDepartment of Internal Medicine Botucatu Medical School Univ Estadual Paulista - UNESPFAPESP: 2014/16406-9Universidade Estadual Paulista (Unesp)Butantan InstituteOlímpio, Regiane Marques Castro [UNESP]Moretto, Fernanda Cristina Fontes [UNESP]De Sibio, Maria Teresa [UNESP]de Oliveira, Miriane [UNESP]Mathias, Lucas Solla [UNESP]Gonçalves, Bianca Mariani [UNESP]Deprá, Igor Carvalho [UNESP]Tilli, Helena Paim. [UNESP]Rodrigues, Bruna Moretto [UNESP]Saraiva, Patrícia Pinto [UNESP]Maria, Durvanei AugustoNogueira, Célia Regina [UNESP]2019-10-06T16:34:57Z2019-10-06T16:34:57Z2019-08-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.lfs.2019.116556Life Sciences, v. 231.1879-06310024-3205http://hdl.handle.net/11449/18925710.1016/j.lfs.2019.1165562-s2.0-85067341769Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2024-08-14T17:22:13Zoai:repositorio.unesp.br:11449/189257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts |
title |
The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts |
spellingShingle |
The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts Olímpio, Regiane Marques Castro [UNESP] Estrogen Osteoblast RANKL and OPG Stem cells Triiodothyronine |
title_short |
The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts |
title_full |
The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts |
title_fullStr |
The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts |
title_full_unstemmed |
The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts |
title_sort |
The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts |
author |
Olímpio, Regiane Marques Castro [UNESP] |
author_facet |
Olímpio, Regiane Marques Castro [UNESP] Moretto, Fernanda Cristina Fontes [UNESP] De Sibio, Maria Teresa [UNESP] de Oliveira, Miriane [UNESP] Mathias, Lucas Solla [UNESP] Gonçalves, Bianca Mariani [UNESP] Deprá, Igor Carvalho [UNESP] Tilli, Helena Paim. [UNESP] Rodrigues, Bruna Moretto [UNESP] Saraiva, Patrícia Pinto [UNESP] Maria, Durvanei Augusto Nogueira, Célia Regina [UNESP] |
author_role |
author |
author2 |
Moretto, Fernanda Cristina Fontes [UNESP] De Sibio, Maria Teresa [UNESP] de Oliveira, Miriane [UNESP] Mathias, Lucas Solla [UNESP] Gonçalves, Bianca Mariani [UNESP] Deprá, Igor Carvalho [UNESP] Tilli, Helena Paim. [UNESP] Rodrigues, Bruna Moretto [UNESP] Saraiva, Patrícia Pinto [UNESP] Maria, Durvanei Augusto Nogueira, Célia Regina [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Butantan Institute |
dc.contributor.author.fl_str_mv |
Olímpio, Regiane Marques Castro [UNESP] Moretto, Fernanda Cristina Fontes [UNESP] De Sibio, Maria Teresa [UNESP] de Oliveira, Miriane [UNESP] Mathias, Lucas Solla [UNESP] Gonçalves, Bianca Mariani [UNESP] Deprá, Igor Carvalho [UNESP] Tilli, Helena Paim. [UNESP] Rodrigues, Bruna Moretto [UNESP] Saraiva, Patrícia Pinto [UNESP] Maria, Durvanei Augusto Nogueira, Célia Regina [UNESP] |
dc.subject.por.fl_str_mv |
Estrogen Osteoblast RANKL and OPG Stem cells Triiodothyronine |
topic |
Estrogen Osteoblast RANKL and OPG Stem cells Triiodothyronine |
description |
Triiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T3. E2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:34:57Z 2019-10-06T16:34:57Z 2019-08-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.lfs.2019.116556 Life Sciences, v. 231. 1879-0631 0024-3205 http://hdl.handle.net/11449/189257 10.1016/j.lfs.2019.116556 2-s2.0-85067341769 |
url |
http://dx.doi.org/10.1016/j.lfs.2019.116556 http://hdl.handle.net/11449/189257 |
identifier_str_mv |
Life Sciences, v. 231. 1879-0631 0024-3205 10.1016/j.lfs.2019.116556 2-s2.0-85067341769 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Life Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128111455240192 |