Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121625 http://hdl.handle.net/11449/129633 |
Resumo: | The present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120 +/- 5.0 g) were anesthetized and 45 inoculated with 20 mu L (40 mg/mL) (2.0 x 10(6) CFU/mg) and five inoculated with saline (0,65%) into muscle tissue in the laterodorsal region. To evaluate the inflammatory process, nine fish inoculated with BCG and one control were sampled in five periods: 3rd, 7th, 14th, 21st and 33rd days post-inoculation (DPI). Immunohistochemical examination showed that the marking with anti-S-100 protein and anti-iNOS antibodies was weak, with a diffuse pattern, between the third and seventh DPI. From the 14th to the 33rd day, the marking became stronger and marked the cytoplasm of the macrophages. Positivity for cytokeratin was initially observed in the 14th DPI, and the stronger immunostaining in the 33rd day, period in which the epithelioid cells were more evident and the granuloma was fully formed. Also after the 14th day, a certain degree of cellular organization was observed, due to the arrangement of the macrophages around the inoculated material, with little evidence of edema. The arrangement of the macrophages around the inoculum, the fibroblasts, the lymphocytes and, in most cases, the presence of melanomacrophages formed the granuloma and kept the inoculum isolated in the 33rd DPI. The present study suggested that the granulomatous experimental model using teleost fish P. mesopotamicus presented a similar response to those observed in mammals, confirming its importance for studies of chronic inflammatory reaction. |
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Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus modelThe present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120 +/- 5.0 g) were anesthetized and 45 inoculated with 20 mu L (40 mg/mL) (2.0 x 10(6) CFU/mg) and five inoculated with saline (0,65%) into muscle tissue in the laterodorsal region. To evaluate the inflammatory process, nine fish inoculated with BCG and one control were sampled in five periods: 3rd, 7th, 14th, 21st and 33rd days post-inoculation (DPI). Immunohistochemical examination showed that the marking with anti-S-100 protein and anti-iNOS antibodies was weak, with a diffuse pattern, between the third and seventh DPI. From the 14th to the 33rd day, the marking became stronger and marked the cytoplasm of the macrophages. Positivity for cytokeratin was initially observed in the 14th DPI, and the stronger immunostaining in the 33rd day, period in which the epithelioid cells were more evident and the granuloma was fully formed. Also after the 14th day, a certain degree of cellular organization was observed, due to the arrangement of the macrophages around the inoculated material, with little evidence of edema. The arrangement of the macrophages around the inoculum, the fibroblasts, the lymphocytes and, in most cases, the presence of melanomacrophages formed the granuloma and kept the inoculum isolated in the 33rd DPI. The present study suggested that the granulomatous experimental model using teleost fish P. mesopotamicus presented a similar response to those observed in mammals, confirming its importance for studies of chronic inflammatory reaction.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Sch Agrarian &Vet Sci, Dept Vet Pathol, Sao Paulo, BrazilUniv Santiago de Compostela, Coll Vet, Dept Pathol Anat, Lugo, SpainSao Paulo State Univ, Sch Agrarian &Vet Sci, Dept Vet Pathol, Sao Paulo, BrazilFAPESP: 2010/086245FAPESP: 2009/17640-7Public Library ScienceUniversidade Estadual Paulista (Unesp)Univ Santiago de CompostelaManrique, Wilson Gomez [UNESP]Claudiano, Gustavo da Silva [UNESP]Castro, Marcello Pardi de [UNESP]Petrillo, Thalita Regina [UNESP]Pereira Figueiredo, Mayra Araguaia [UNESP]Andrade Belo, Marco Antonio de [UNESP]Quiroga Berdeal, Maria IsabelRodini de Moraes, Julieta Engracia [UNESP]Moraes, Flavio Ruas de [UNESP]2015-10-22T06:20:11Z2015-10-22T06:20:11Z2015-03-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8application/pdfhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121625Plos One. San Francisco: Public Library Science, v. 10, n. 3, p. 1-8, 2015.1932-6203http://hdl.handle.net/11449/12963310.1371/journal.pone.0121625WOS:000356353700123WOS000356353700123.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One2.7661,164info:eu-repo/semantics/openAccess2021-10-23T21:50:45Zoai:repositorio.unesp.br:11449/129633Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T21:50:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model |
title |
Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model |
spellingShingle |
Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model Manrique, Wilson Gomez [UNESP] |
title_short |
Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model |
title_full |
Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model |
title_fullStr |
Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model |
title_full_unstemmed |
Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model |
title_sort |
Expression of cellular components in granulomatous inflammatory response in piaractus mesopotamicus model |
author |
Manrique, Wilson Gomez [UNESP] |
author_facet |
Manrique, Wilson Gomez [UNESP] Claudiano, Gustavo da Silva [UNESP] Castro, Marcello Pardi de [UNESP] Petrillo, Thalita Regina [UNESP] Pereira Figueiredo, Mayra Araguaia [UNESP] Andrade Belo, Marco Antonio de [UNESP] Quiroga Berdeal, Maria Isabel Rodini de Moraes, Julieta Engracia [UNESP] Moraes, Flavio Ruas de [UNESP] |
author_role |
author |
author2 |
Claudiano, Gustavo da Silva [UNESP] Castro, Marcello Pardi de [UNESP] Petrillo, Thalita Regina [UNESP] Pereira Figueiredo, Mayra Araguaia [UNESP] Andrade Belo, Marco Antonio de [UNESP] Quiroga Berdeal, Maria Isabel Rodini de Moraes, Julieta Engracia [UNESP] Moraes, Flavio Ruas de [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Santiago de Compostela |
dc.contributor.author.fl_str_mv |
Manrique, Wilson Gomez [UNESP] Claudiano, Gustavo da Silva [UNESP] Castro, Marcello Pardi de [UNESP] Petrillo, Thalita Regina [UNESP] Pereira Figueiredo, Mayra Araguaia [UNESP] Andrade Belo, Marco Antonio de [UNESP] Quiroga Berdeal, Maria Isabel Rodini de Moraes, Julieta Engracia [UNESP] Moraes, Flavio Ruas de [UNESP] |
description |
The present study aimed to describe and characterize the cellular components during the evolution of chronic granulomatous inflammation in the teleost fish pacus (P. mesopotamicus) induced by Bacillus Calmette-Guerin (BCG), using S-100, iNOS and cytokeratin antibodies. 50 fish (120 +/- 5.0 g) were anesthetized and 45 inoculated with 20 mu L (40 mg/mL) (2.0 x 10(6) CFU/mg) and five inoculated with saline (0,65%) into muscle tissue in the laterodorsal region. To evaluate the inflammatory process, nine fish inoculated with BCG and one control were sampled in five periods: 3rd, 7th, 14th, 21st and 33rd days post-inoculation (DPI). Immunohistochemical examination showed that the marking with anti-S-100 protein and anti-iNOS antibodies was weak, with a diffuse pattern, between the third and seventh DPI. From the 14th to the 33rd day, the marking became stronger and marked the cytoplasm of the macrophages. Positivity for cytokeratin was initially observed in the 14th DPI, and the stronger immunostaining in the 33rd day, period in which the epithelioid cells were more evident and the granuloma was fully formed. Also after the 14th day, a certain degree of cellular organization was observed, due to the arrangement of the macrophages around the inoculated material, with little evidence of edema. The arrangement of the macrophages around the inoculum, the fibroblasts, the lymphocytes and, in most cases, the presence of melanomacrophages formed the granuloma and kept the inoculum isolated in the 33rd DPI. The present study suggested that the granulomatous experimental model using teleost fish P. mesopotamicus presented a similar response to those observed in mammals, confirming its importance for studies of chronic inflammatory reaction. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-22T06:20:11Z 2015-10-22T06:20:11Z 2015-03-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121625 Plos One. San Francisco: Public Library Science, v. 10, n. 3, p. 1-8, 2015. 1932-6203 http://hdl.handle.net/11449/129633 10.1371/journal.pone.0121625 WOS:000356353700123 WOS000356353700123.pdf |
url |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121625 http://hdl.handle.net/11449/129633 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 10, n. 3, p. 1-8, 2015. 1932-6203 10.1371/journal.pone.0121625 WOS:000356353700123 WOS000356353700123.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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