Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2018.02.020 http://hdl.handle.net/11449/179619 |
Resumo: | This study aimed to evaluate the antioxidant properties of coenzyme Q10 (CoQ10) during cryopreservation of semen obtained from stallions having good and bad semen freezing ability (GFA vs. BFA, respectively). Forty ejaculates (n = 20 stallions) were split into five centrifugation and five freezing extenders containing different concentrations of CoQ10 (0, 25, 50, 75 and 100 μmols/L). If CoQ10 was added to the centrifugation extender, the freezing extender had no CoQ10 added; similarly, if CoQ10 was added to the freezing extender, the centrifugation extender had no CoQ10. Semen cryopreserved on extenders containing no CoQ10 served as the control. After post-thaw total sperm motility (TM) assessments, the stallions were classified as GFA (i.e., decrease of ≤25% in TM, n = 7) or BFA (i.e., decrease of ≥40% in TM, n = 5). Stallions not fitting (n = 8) this enrollment criteria had samples discarded. After that, two straws for each extender were thawed at 37 °C for 30 s; one straw was immediately used for evaluation of sperm kinetics, plasma membrane integrity, non-capacitated spermatozoa, reactive oxygen species production, mitochondrial activity and lipid peroxidation. The second straw was kept at 37 °C for 30 min and subjected to the same assessments. Expectedly, sperm motility parameters were significantly lower for stallions with BFA. There were no effects of CoQ10 concentration or time for all parameters evaluated in the group with GFA when compared with the control extender (p > 0.05), except lipid peroxidation (p < 0.05). However, stallions with BFA had improved sperm parameters for samples processed with extenders containing CoQ10 (particularly 75 μmols/L) (p < 0.05), except for the reactive oxygen species production and mitochondrial potential (T0) in which there were no differences between the groups (p > 0.05). In summary, 75 μmols/L appears to be the optimal dose of Co-Q10, particularly, when added to the centrifugation extender. |
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Repositório Institucional da UNESP |
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Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing abilityAntioxidantCryodamageHorseOxidative stressSemen freezingThis study aimed to evaluate the antioxidant properties of coenzyme Q10 (CoQ10) during cryopreservation of semen obtained from stallions having good and bad semen freezing ability (GFA vs. BFA, respectively). Forty ejaculates (n = 20 stallions) were split into five centrifugation and five freezing extenders containing different concentrations of CoQ10 (0, 25, 50, 75 and 100 μmols/L). If CoQ10 was added to the centrifugation extender, the freezing extender had no CoQ10 added; similarly, if CoQ10 was added to the freezing extender, the centrifugation extender had no CoQ10. Semen cryopreserved on extenders containing no CoQ10 served as the control. After post-thaw total sperm motility (TM) assessments, the stallions were classified as GFA (i.e., decrease of ≤25% in TM, n = 7) or BFA (i.e., decrease of ≥40% in TM, n = 5). Stallions not fitting (n = 8) this enrollment criteria had samples discarded. After that, two straws for each extender were thawed at 37 °C for 30 s; one straw was immediately used for evaluation of sperm kinetics, plasma membrane integrity, non-capacitated spermatozoa, reactive oxygen species production, mitochondrial activity and lipid peroxidation. The second straw was kept at 37 °C for 30 min and subjected to the same assessments. Expectedly, sperm motility parameters were significantly lower for stallions with BFA. There were no effects of CoQ10 concentration or time for all parameters evaluated in the group with GFA when compared with the control extender (p > 0.05), except lipid peroxidation (p < 0.05). However, stallions with BFA had improved sperm parameters for samples processed with extenders containing CoQ10 (particularly 75 μmols/L) (p < 0.05), except for the reactive oxygen species production and mitochondrial potential (T0) in which there were no differences between the groups (p > 0.05). In summary, 75 μmols/L appears to be the optimal dose of Co-Q10, particularly, when added to the centrifugation extender.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State University (UNESP) School of Veterinary Medicine and Animal ScienceDepartment of Veterinary Clinical Medicine College of Veterinary Medicine University of Illinois Urbana-ChampaignSao Paulo State University (UNESP) School of Veterinary Medicine and Animal ScienceFAPESP: 2015/25638-3Universidade Estadual Paulista (Unesp)University of Illinois Urbana-ChampaignCarneiro, Joao A.M. [UNESP]Canisso, Igor F.Bandeira, R. S. [UNESP]Scheeren, V. F.C. [UNESP]Freitas-Dell'Aqua, Camila P. [UNESP]Alvarenga, Marco A. [UNESP]Papa, Frederico O. [UNESP]Dell'Aqua, Jose A. [UNESP]2018-12-11T17:36:03Z2018-12-11T17:36:03Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article107-118application/pdfhttp://dx.doi.org/10.1016/j.anireprosci.2018.02.020Animal Reproduction Science, v. 192, p. 107-118.0378-4320http://hdl.handle.net/11449/17961910.1016/j.anireprosci.2018.02.0202-s2.0-850426182612-s2.0-85042618261.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Science0,704info:eu-repo/semantics/openAccess2023-11-25T06:16:57Zoai:repositorio.unesp.br:11449/179619Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-25T06:16:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability |
title |
Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability |
spellingShingle |
Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability Carneiro, Joao A.M. [UNESP] Antioxidant Cryodamage Horse Oxidative stress Semen freezing |
title_short |
Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability |
title_full |
Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability |
title_fullStr |
Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability |
title_full_unstemmed |
Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability |
title_sort |
Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability |
author |
Carneiro, Joao A.M. [UNESP] |
author_facet |
Carneiro, Joao A.M. [UNESP] Canisso, Igor F. Bandeira, R. S. [UNESP] Scheeren, V. F.C. [UNESP] Freitas-Dell'Aqua, Camila P. [UNESP] Alvarenga, Marco A. [UNESP] Papa, Frederico O. [UNESP] Dell'Aqua, Jose A. [UNESP] |
author_role |
author |
author2 |
Canisso, Igor F. Bandeira, R. S. [UNESP] Scheeren, V. F.C. [UNESP] Freitas-Dell'Aqua, Camila P. [UNESP] Alvarenga, Marco A. [UNESP] Papa, Frederico O. [UNESP] Dell'Aqua, Jose A. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Illinois Urbana-Champaign |
dc.contributor.author.fl_str_mv |
Carneiro, Joao A.M. [UNESP] Canisso, Igor F. Bandeira, R. S. [UNESP] Scheeren, V. F.C. [UNESP] Freitas-Dell'Aqua, Camila P. [UNESP] Alvarenga, Marco A. [UNESP] Papa, Frederico O. [UNESP] Dell'Aqua, Jose A. [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant Cryodamage Horse Oxidative stress Semen freezing |
topic |
Antioxidant Cryodamage Horse Oxidative stress Semen freezing |
description |
This study aimed to evaluate the antioxidant properties of coenzyme Q10 (CoQ10) during cryopreservation of semen obtained from stallions having good and bad semen freezing ability (GFA vs. BFA, respectively). Forty ejaculates (n = 20 stallions) were split into five centrifugation and five freezing extenders containing different concentrations of CoQ10 (0, 25, 50, 75 and 100 μmols/L). If CoQ10 was added to the centrifugation extender, the freezing extender had no CoQ10 added; similarly, if CoQ10 was added to the freezing extender, the centrifugation extender had no CoQ10. Semen cryopreserved on extenders containing no CoQ10 served as the control. After post-thaw total sperm motility (TM) assessments, the stallions were classified as GFA (i.e., decrease of ≤25% in TM, n = 7) or BFA (i.e., decrease of ≥40% in TM, n = 5). Stallions not fitting (n = 8) this enrollment criteria had samples discarded. After that, two straws for each extender were thawed at 37 °C for 30 s; one straw was immediately used for evaluation of sperm kinetics, plasma membrane integrity, non-capacitated spermatozoa, reactive oxygen species production, mitochondrial activity and lipid peroxidation. The second straw was kept at 37 °C for 30 min and subjected to the same assessments. Expectedly, sperm motility parameters were significantly lower for stallions with BFA. There were no effects of CoQ10 concentration or time for all parameters evaluated in the group with GFA when compared with the control extender (p > 0.05), except lipid peroxidation (p < 0.05). However, stallions with BFA had improved sperm parameters for samples processed with extenders containing CoQ10 (particularly 75 μmols/L) (p < 0.05), except for the reactive oxygen species production and mitochondrial potential (T0) in which there were no differences between the groups (p > 0.05). In summary, 75 μmols/L appears to be the optimal dose of Co-Q10, particularly, when added to the centrifugation extender. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:36:03Z 2018-12-11T17:36:03Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2018.02.020 Animal Reproduction Science, v. 192, p. 107-118. 0378-4320 http://hdl.handle.net/11449/179619 10.1016/j.anireprosci.2018.02.020 2-s2.0-85042618261 2-s2.0-85042618261.pdf |
url |
http://dx.doi.org/10.1016/j.anireprosci.2018.02.020 http://hdl.handle.net/11449/179619 |
identifier_str_mv |
Animal Reproduction Science, v. 192, p. 107-118. 0378-4320 10.1016/j.anireprosci.2018.02.020 2-s2.0-85042618261 2-s2.0-85042618261.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science 0,704 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
107-118 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965067038425088 |