Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3109/13880200903122202 http://hdl.handle.net/11449/225777 |
Resumo: | This study evaluated the influence of Psidium cattleianum Sabine (Myrtaceae) and Myracrodruon urundeuva Allemão (Anacardiaceae) aqueous extracts on S. mutans counts and dental enamel micro-hardness of rats submitted to a cariogenic challenge. Sixty Wistar rats were distributed in three groups and received water (control) or aqueous extracts of Psidium cattleianum or Myracrodruon urundeuva as hydration solution. Initially the animals had their sublingual and submandibular salivary glands surgically removed and the parotid ducts ligated. Then the rats were inoculated with 10 6 CFU of Streptococcus mutans ATCC 35668 and were fed with a cariogenic diet. To detect and quantify the presence of S. mutans, oral biofilms were sampled and microbial DNA was extracted and submitted to amplification by means of real-time PCR (Polymerase Chain Reaction). After seven weeks the animals were sacrificed and enamel demineralization was analyzed by cross-sectional micro-hardness. Both extracts produced a significant reduction on S. mutans counts and decreased the enamel demineralization. It can be concluded that the extracts tested had a significant effect on S. mutans in oral biofilm of the rats, decreasing S. mutans accumulation and enamel demineralization. © 2010 Informa UK Ltd. |
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Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in ratsAntibacterial agentsDental cariesNatural productsStreptococcus mutansThis study evaluated the influence of Psidium cattleianum Sabine (Myrtaceae) and Myracrodruon urundeuva Allemão (Anacardiaceae) aqueous extracts on S. mutans counts and dental enamel micro-hardness of rats submitted to a cariogenic challenge. Sixty Wistar rats were distributed in three groups and received water (control) or aqueous extracts of Psidium cattleianum or Myracrodruon urundeuva as hydration solution. Initially the animals had their sublingual and submandibular salivary glands surgically removed and the parotid ducts ligated. Then the rats were inoculated with 10 6 CFU of Streptococcus mutans ATCC 35668 and were fed with a cariogenic diet. To detect and quantify the presence of S. mutans, oral biofilms were sampled and microbial DNA was extracted and submitted to amplification by means of real-time PCR (Polymerase Chain Reaction). After seven weeks the animals were sacrificed and enamel demineralization was analyzed by cross-sectional micro-hardness. Both extracts produced a significant reduction on S. mutans counts and decreased the enamel demineralization. It can be concluded that the extracts tested had a significant effect on S. mutans in oral biofilm of the rats, decreasing S. mutans accumulation and enamel demineralization. © 2010 Informa UK Ltd.Department of Pediatric Dentistry São Paulo State University UNESP, AraçatubaDepartment of Pathology and Oral Diagnostics São Paulo State University UNESP, AraçatubaDepartment of Pediatric Dentistry São Paulo State University UNESP, AraçatubaDepartment of Pathology and Oral Diagnostics São Paulo State University UNESP, AraçatubaUniversidade Estadual Paulista (UNESP)Crivelaro De Menezes, Taís Elisabete [UNESP]Botazzo Delbem, Alberto Carlos [UNESP]Lourenção Brighenti, Fernanda [UNESP]Cluádia Okamoto, Ana [UNESP]Gaetti-Jardim Jr., Elerson [UNESP]2022-04-28T20:59:35Z2022-04-28T20:59:35Z2010-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article300-305http://dx.doi.org/10.3109/13880200903122202Pharmaceutical Biology, v. 48, n. 3, p. 300-305, 2010.1388-02091744-5116http://hdl.handle.net/11449/22577710.3109/138802009031222022-s2.0-77249106488Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPharmaceutical Biologyinfo:eu-repo/semantics/openAccess2022-04-28T20:59:36Zoai:repositorio.unesp.br:11449/225777Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T20:59:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats |
title |
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats |
spellingShingle |
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats Crivelaro De Menezes, Taís Elisabete [UNESP] Antibacterial agents Dental caries Natural products Streptococcus mutans |
title_short |
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats |
title_full |
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats |
title_fullStr |
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats |
title_full_unstemmed |
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats |
title_sort |
Protective efficacy of Psidium cattleianum and Myracrodruon urundeuva aqueous extracts against caries development in rats |
author |
Crivelaro De Menezes, Taís Elisabete [UNESP] |
author_facet |
Crivelaro De Menezes, Taís Elisabete [UNESP] Botazzo Delbem, Alberto Carlos [UNESP] Lourenção Brighenti, Fernanda [UNESP] Cluádia Okamoto, Ana [UNESP] Gaetti-Jardim Jr., Elerson [UNESP] |
author_role |
author |
author2 |
Botazzo Delbem, Alberto Carlos [UNESP] Lourenção Brighenti, Fernanda [UNESP] Cluádia Okamoto, Ana [UNESP] Gaetti-Jardim Jr., Elerson [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Crivelaro De Menezes, Taís Elisabete [UNESP] Botazzo Delbem, Alberto Carlos [UNESP] Lourenção Brighenti, Fernanda [UNESP] Cluádia Okamoto, Ana [UNESP] Gaetti-Jardim Jr., Elerson [UNESP] |
dc.subject.por.fl_str_mv |
Antibacterial agents Dental caries Natural products Streptococcus mutans |
topic |
Antibacterial agents Dental caries Natural products Streptococcus mutans |
description |
This study evaluated the influence of Psidium cattleianum Sabine (Myrtaceae) and Myracrodruon urundeuva Allemão (Anacardiaceae) aqueous extracts on S. mutans counts and dental enamel micro-hardness of rats submitted to a cariogenic challenge. Sixty Wistar rats were distributed in three groups and received water (control) or aqueous extracts of Psidium cattleianum or Myracrodruon urundeuva as hydration solution. Initially the animals had their sublingual and submandibular salivary glands surgically removed and the parotid ducts ligated. Then the rats were inoculated with 10 6 CFU of Streptococcus mutans ATCC 35668 and were fed with a cariogenic diet. To detect and quantify the presence of S. mutans, oral biofilms were sampled and microbial DNA was extracted and submitted to amplification by means of real-time PCR (Polymerase Chain Reaction). After seven weeks the animals were sacrificed and enamel demineralization was analyzed by cross-sectional micro-hardness. Both extracts produced a significant reduction on S. mutans counts and decreased the enamel demineralization. It can be concluded that the extracts tested had a significant effect on S. mutans in oral biofilm of the rats, decreasing S. mutans accumulation and enamel demineralization. © 2010 Informa UK Ltd. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01 2022-04-28T20:59:35Z 2022-04-28T20:59:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3109/13880200903122202 Pharmaceutical Biology, v. 48, n. 3, p. 300-305, 2010. 1388-0209 1744-5116 http://hdl.handle.net/11449/225777 10.3109/13880200903122202 2-s2.0-77249106488 |
url |
http://dx.doi.org/10.3109/13880200903122202 http://hdl.handle.net/11449/225777 |
identifier_str_mv |
Pharmaceutical Biology, v. 48, n. 3, p. 300-305, 2010. 1388-0209 1744-5116 10.3109/13880200903122202 2-s2.0-77249106488 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pharmaceutical Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
300-305 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965712436953088 |