Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fpls.2022.1009350 http://hdl.handle.net/11449/245981 |
Resumo: | Huanglongbing (HLB), the most destructive citrus disease, is associated with unculturable, phloem-limited Candidatus Liberibacter species, mainly Ca. L. asiaticus (Las). Las is transmitted naturally by the insect Diaphorina citri. In a previous study, we determined that the Oceanian citrus relatives Eremocitrus glauca, Microcitrus warburgiana, Microcitrus papuana, and Microcitrus australis and three hybrids among them and Citrus were full-resistant to Las. After 2 years of evaluations, leaves of those seven genotypes remained Las-free even with their susceptible rootstock being infected. However, Las was detected in their stem bark above the scion-rootstock graft union. Aiming to gain an understanding of the full-resistance phenotype, new experiments were carried out with the challenge-inoculated Oceanian citrus genotypes through which we evaluated: (1) Las acquisition by D. citri fed onto them; (2) Las infection in sweet orange plants grafted with bark or budwood from them; (3) Las infection in sweet orange plants top-grafted onto them; (4) Las infection in new shoots from rooted plants of them; and (5) Las infection in new shoots of them after drastic back-pruning. Overall, results showed that insects that fed on plants from the Oceanian citrus genotypes, their canopies, new flushes, and leaves from rooted cuttings evaluated remained quantitative real-time polymerase chain reaction (qPCR)-negative. Moreover, their budwood pieces were unable to infect sweet orange through grafting. Furthermore, sweet orange control leaves resulted infected when insects fed onto them and graft-receptor susceptible plants. Genomic and morphological analysis of the Oceanian genotypes corroborated that E. glauca and M. warburgiana are pure species while our M. australis accession is an M. australis × M. inodora hybrid and M. papuana is probably a M. papuana × M. warburgiana hybrid. E. glauca × C. sinensis hybrid was found coming from a cross between E. glauca and mandarin or tangor. Eremocitrus × Microcitrus hybrid is a complex admixture of M. australasica, M. australis, and E. glauca while the last hybrid is an M. australasica × M. australis admixture. Confirmation of consistent full resistance in these genotypes with proper validation of their genomic parentages is essential to map properly genomic regions for breeding programs aimed to generate new Citrus-like cultivars yielding immunity to HLB. |
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Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypesAurantioideaecitrus breedingEremocitrusgreeningHLBMicrocitrusRutaceaeHuanglongbing (HLB), the most destructive citrus disease, is associated with unculturable, phloem-limited Candidatus Liberibacter species, mainly Ca. L. asiaticus (Las). Las is transmitted naturally by the insect Diaphorina citri. In a previous study, we determined that the Oceanian citrus relatives Eremocitrus glauca, Microcitrus warburgiana, Microcitrus papuana, and Microcitrus australis and three hybrids among them and Citrus were full-resistant to Las. After 2 years of evaluations, leaves of those seven genotypes remained Las-free even with their susceptible rootstock being infected. However, Las was detected in their stem bark above the scion-rootstock graft union. Aiming to gain an understanding of the full-resistance phenotype, new experiments were carried out with the challenge-inoculated Oceanian citrus genotypes through which we evaluated: (1) Las acquisition by D. citri fed onto them; (2) Las infection in sweet orange plants grafted with bark or budwood from them; (3) Las infection in sweet orange plants top-grafted onto them; (4) Las infection in new shoots from rooted plants of them; and (5) Las infection in new shoots of them after drastic back-pruning. Overall, results showed that insects that fed on plants from the Oceanian citrus genotypes, their canopies, new flushes, and leaves from rooted cuttings evaluated remained quantitative real-time polymerase chain reaction (qPCR)-negative. Moreover, their budwood pieces were unable to infect sweet orange through grafting. Furthermore, sweet orange control leaves resulted infected when insects fed onto them and graft-receptor susceptible plants. Genomic and morphological analysis of the Oceanian genotypes corroborated that E. glauca and M. warburgiana are pure species while our M. australis accession is an M. australis × M. inodora hybrid and M. papuana is probably a M. papuana × M. warburgiana hybrid. E. glauca × C. sinensis hybrid was found coming from a cross between E. glauca and mandarin or tangor. Eremocitrus × Microcitrus hybrid is a complex admixture of M. australasica, M. australis, and E. glauca while the last hybrid is an M. australasica × M. australis admixture. Confirmation of consistent full resistance in these genotypes with proper validation of their genomic parentages is essential to map properly genomic regions for breeding programs aimed to generate new Citrus-like cultivars yielding immunity to HLB.Fundo de Defesa da CitriculturaFundo de Defesa da CitriculturaFaculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa AgropecuáriaCIRAD UMR AGAP InstitutAGAP Institut Univ. Montpellier CIRAD INRAE Institut AgroInstituto de Biologia Molecular y Celular de Plantas – Consejo Superior de Investigaciones Científicas Universidad Politécnica de ValenciaFaculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista (UNESP)Fundo de Defesa da CitriculturaUniversidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)UMR AGAP InstitutInstitut AgroUniversidad Politécnica de ValenciaAlves, Mônica N. [UNESP]Raiol-Junior, Laudecir L.Girardi, Eduardo A.Miranda, MaévaWulff, Nelson A.Carvalho, Everton V.Lopes, Sílvio A.Ferro, Jesus A. [UNESP]Ollitrault, PatrickPeña, Leandro2023-07-29T12:28:33Z2023-07-29T12:28:33Z2022-09-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fpls.2022.1009350Frontiers in Plant Science, v. 13.1664-462Xhttp://hdl.handle.net/11449/24598110.3389/fpls.2022.10093502-s2.0-85138831014Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Plant Scienceinfo:eu-repo/semantics/openAccess2023-07-29T12:28:33Zoai:repositorio.unesp.br:11449/245981Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:28:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes |
title |
Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes |
spellingShingle |
Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes Alves, Mônica N. [UNESP] Aurantioideae citrus breeding Eremocitrus greening HLB Microcitrus Rutaceae |
title_short |
Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes |
title_full |
Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes |
title_fullStr |
Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes |
title_full_unstemmed |
Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes |
title_sort |
Insight into resistance to ‘Candidatus Liberibacter asiaticus,’ associated with Huanglongbing, in Oceanian citrus genotypes |
author |
Alves, Mônica N. [UNESP] |
author_facet |
Alves, Mônica N. [UNESP] Raiol-Junior, Laudecir L. Girardi, Eduardo A. Miranda, Maéva Wulff, Nelson A. Carvalho, Everton V. Lopes, Sílvio A. Ferro, Jesus A. [UNESP] Ollitrault, Patrick Peña, Leandro |
author_role |
author |
author2 |
Raiol-Junior, Laudecir L. Girardi, Eduardo A. Miranda, Maéva Wulff, Nelson A. Carvalho, Everton V. Lopes, Sílvio A. Ferro, Jesus A. [UNESP] Ollitrault, Patrick Peña, Leandro |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Fundo de Defesa da Citricultura Universidade Estadual Paulista (UNESP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) UMR AGAP Institut Institut Agro Universidad Politécnica de Valencia |
dc.contributor.author.fl_str_mv |
Alves, Mônica N. [UNESP] Raiol-Junior, Laudecir L. Girardi, Eduardo A. Miranda, Maéva Wulff, Nelson A. Carvalho, Everton V. Lopes, Sílvio A. Ferro, Jesus A. [UNESP] Ollitrault, Patrick Peña, Leandro |
dc.subject.por.fl_str_mv |
Aurantioideae citrus breeding Eremocitrus greening HLB Microcitrus Rutaceae |
topic |
Aurantioideae citrus breeding Eremocitrus greening HLB Microcitrus Rutaceae |
description |
Huanglongbing (HLB), the most destructive citrus disease, is associated with unculturable, phloem-limited Candidatus Liberibacter species, mainly Ca. L. asiaticus (Las). Las is transmitted naturally by the insect Diaphorina citri. In a previous study, we determined that the Oceanian citrus relatives Eremocitrus glauca, Microcitrus warburgiana, Microcitrus papuana, and Microcitrus australis and three hybrids among them and Citrus were full-resistant to Las. After 2 years of evaluations, leaves of those seven genotypes remained Las-free even with their susceptible rootstock being infected. However, Las was detected in their stem bark above the scion-rootstock graft union. Aiming to gain an understanding of the full-resistance phenotype, new experiments were carried out with the challenge-inoculated Oceanian citrus genotypes through which we evaluated: (1) Las acquisition by D. citri fed onto them; (2) Las infection in sweet orange plants grafted with bark or budwood from them; (3) Las infection in sweet orange plants top-grafted onto them; (4) Las infection in new shoots from rooted plants of them; and (5) Las infection in new shoots of them after drastic back-pruning. Overall, results showed that insects that fed on plants from the Oceanian citrus genotypes, their canopies, new flushes, and leaves from rooted cuttings evaluated remained quantitative real-time polymerase chain reaction (qPCR)-negative. Moreover, their budwood pieces were unable to infect sweet orange through grafting. Furthermore, sweet orange control leaves resulted infected when insects fed onto them and graft-receptor susceptible plants. Genomic and morphological analysis of the Oceanian genotypes corroborated that E. glauca and M. warburgiana are pure species while our M. australis accession is an M. australis × M. inodora hybrid and M. papuana is probably a M. papuana × M. warburgiana hybrid. E. glauca × C. sinensis hybrid was found coming from a cross between E. glauca and mandarin or tangor. Eremocitrus × Microcitrus hybrid is a complex admixture of M. australasica, M. australis, and E. glauca while the last hybrid is an M. australasica × M. australis admixture. Confirmation of consistent full resistance in these genotypes with proper validation of their genomic parentages is essential to map properly genomic regions for breeding programs aimed to generate new Citrus-like cultivars yielding immunity to HLB. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-09 2023-07-29T12:28:33Z 2023-07-29T12:28:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fpls.2022.1009350 Frontiers in Plant Science, v. 13. 1664-462X http://hdl.handle.net/11449/245981 10.3389/fpls.2022.1009350 2-s2.0-85138831014 |
url |
http://dx.doi.org/10.3389/fpls.2022.1009350 http://hdl.handle.net/11449/245981 |
identifier_str_mv |
Frontiers in Plant Science, v. 13. 1664-462X 10.3389/fpls.2022.1009350 2-s2.0-85138831014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Plant Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965725308223488 |