Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10499-017-0204-4 http://hdl.handle.net/11449/175309 |
Resumo: | Francisella noatunensis orientalis is a bacterium that causes emerging bacteriosis in Nile tilapia (Oreochromis niloticus) in many parts of the world, including Brazil. It is a non-motile, Gram-negative, strictly aerobic, facultative intracellular coccobacillus. This species of bacteria is responsible for low to high mortality in fish farms, causing economic losses for fish farmers. This study aimed to detect the presence of F. noatunensis orientalis using qPCR (real-time polymerase chain reaction) and to describe lesions caused by the bacterium in O. niloticus in Brazilian aquaculture. For this purpose, 360 fish from six fish farms (30 per farm) were sampled at two time points (n = 180 per sampling). Necropsies and histopathology were performed for lesion observation, in addition to qPCR and sequencing for detection and identification of Francisella species. Environmental data were collected using a multiparameter sonde YSI EXO2. All measured limnological variables were within the optimum range for cultivation of Nile tilapia. The major lesions present were melanization of the skin, splenomegaly, granulomas, and inflammatory cell responses. The prevalence of francisellosis varied from 0 to 86.66% between time periods and fish farms analyzed, and an outbreak was observed during the second sampling period. This study describes the prevalence of francisellosis in O. niloticus and reports that the lesions found are not exclusively associated with this bacterial disease. |
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Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in BrazilBacterial diseasesFrancisellosisOutbreakqPCRFrancisella noatunensis orientalis is a bacterium that causes emerging bacteriosis in Nile tilapia (Oreochromis niloticus) in many parts of the world, including Brazil. It is a non-motile, Gram-negative, strictly aerobic, facultative intracellular coccobacillus. This species of bacteria is responsible for low to high mortality in fish farms, causing economic losses for fish farmers. This study aimed to detect the presence of F. noatunensis orientalis using qPCR (real-time polymerase chain reaction) and to describe lesions caused by the bacterium in O. niloticus in Brazilian aquaculture. For this purpose, 360 fish from six fish farms (30 per farm) were sampled at two time points (n = 180 per sampling). Necropsies and histopathology were performed for lesion observation, in addition to qPCR and sequencing for detection and identification of Francisella species. Environmental data were collected using a multiparameter sonde YSI EXO2. All measured limnological variables were within the optimum range for cultivation of Nile tilapia. The major lesions present were melanization of the skin, splenomegaly, granulomas, and inflammatory cell responses. The prevalence of francisellosis varied from 0 to 86.66% between time periods and fish farms analyzed, and an outbreak was observed during the second sampling period. This study describes the prevalence of francisellosis in O. niloticus and reports that the lesions found are not exclusively associated with this bacterial disease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Microbiology and Immunology Biosciences Institute Univ. Estadual Paulista (UNESP), Distrito de Rubião JúniorDepartment of Parasitology Biosciences Institute Univ. Estadual Paulista (UNESP), Distrito de Rubião JúniorSão Paulo State Agency for Agribusiness Technology (APTA), CP 66 JaúDepartment of Microbiology and Immunology Biosciences Institute Univ. Estadual Paulista (UNESP), Distrito de Rubião JúniorDepartment of Parasitology Biosciences Institute Univ. Estadual Paulista (UNESP), Distrito de Rubião JúniorFAPESP: 2013/50504-5FAPESP: 2014/13718-0FAPESP: 2014/15859-0FAPESP: 2015/13025-7FAPESP: 2016/50377-1CNPq: 307808/2014-9CNPq: 440496/2015-2Universidade Estadual Paulista (Unesp)São Paulo State Agency for Agribusiness Technology (APTA)Rodrigues, Marianna Vaz [UNESP]Francisco, Claire Juliana [UNESP]David, Gianmarco S.da Silva, Reinaldo José [UNESP]Falcone-Dias, Maria FernandaJúnior, João Pessoa Araújo [UNESP]2018-12-11T17:15:14Z2018-12-11T17:15:14Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article127-138application/pdfhttp://dx.doi.org/10.1007/s10499-017-0204-4Aquaculture International, v. 26, n. 1, p. 127-138, 2018.1573-143X0967-6120http://hdl.handle.net/11449/17530910.1007/s10499-017-0204-42-s2.0-850307206372-s2.0-85030720637.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture International0,591info:eu-repo/semantics/openAccess2023-10-12T06:04:38Zoai:repositorio.unesp.br:11449/175309Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-12T06:04:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil |
title |
Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil |
spellingShingle |
Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil Rodrigues, Marianna Vaz [UNESP] Bacterial diseases Francisellosis Outbreak qPCR |
title_short |
Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil |
title_full |
Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil |
title_fullStr |
Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil |
title_full_unstemmed |
Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil |
title_sort |
Monitoring of Francisella noatunensis subsp. orientalis in farmed Nile tilapia (Oreochromis niloticus) in Brazil |
author |
Rodrigues, Marianna Vaz [UNESP] |
author_facet |
Rodrigues, Marianna Vaz [UNESP] Francisco, Claire Juliana [UNESP] David, Gianmarco S. da Silva, Reinaldo José [UNESP] Falcone-Dias, Maria Fernanda Júnior, João Pessoa Araújo [UNESP] |
author_role |
author |
author2 |
Francisco, Claire Juliana [UNESP] David, Gianmarco S. da Silva, Reinaldo José [UNESP] Falcone-Dias, Maria Fernanda Júnior, João Pessoa Araújo [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) São Paulo State Agency for Agribusiness Technology (APTA) |
dc.contributor.author.fl_str_mv |
Rodrigues, Marianna Vaz [UNESP] Francisco, Claire Juliana [UNESP] David, Gianmarco S. da Silva, Reinaldo José [UNESP] Falcone-Dias, Maria Fernanda Júnior, João Pessoa Araújo [UNESP] |
dc.subject.por.fl_str_mv |
Bacterial diseases Francisellosis Outbreak qPCR |
topic |
Bacterial diseases Francisellosis Outbreak qPCR |
description |
Francisella noatunensis orientalis is a bacterium that causes emerging bacteriosis in Nile tilapia (Oreochromis niloticus) in many parts of the world, including Brazil. It is a non-motile, Gram-negative, strictly aerobic, facultative intracellular coccobacillus. This species of bacteria is responsible for low to high mortality in fish farms, causing economic losses for fish farmers. This study aimed to detect the presence of F. noatunensis orientalis using qPCR (real-time polymerase chain reaction) and to describe lesions caused by the bacterium in O. niloticus in Brazilian aquaculture. For this purpose, 360 fish from six fish farms (30 per farm) were sampled at two time points (n = 180 per sampling). Necropsies and histopathology were performed for lesion observation, in addition to qPCR and sequencing for detection and identification of Francisella species. Environmental data were collected using a multiparameter sonde YSI EXO2. All measured limnological variables were within the optimum range for cultivation of Nile tilapia. The major lesions present were melanization of the skin, splenomegaly, granulomas, and inflammatory cell responses. The prevalence of francisellosis varied from 0 to 86.66% between time periods and fish farms analyzed, and an outbreak was observed during the second sampling period. This study describes the prevalence of francisellosis in O. niloticus and reports that the lesions found are not exclusively associated with this bacterial disease. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:15:14Z 2018-12-11T17:15:14Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10499-017-0204-4 Aquaculture International, v. 26, n. 1, p. 127-138, 2018. 1573-143X 0967-6120 http://hdl.handle.net/11449/175309 10.1007/s10499-017-0204-4 2-s2.0-85030720637 2-s2.0-85030720637.pdf |
url |
http://dx.doi.org/10.1007/s10499-017-0204-4 http://hdl.handle.net/11449/175309 |
identifier_str_mv |
Aquaculture International, v. 26, n. 1, p. 127-138, 2018. 1573-143X 0967-6120 10.1007/s10499-017-0204-4 2-s2.0-85030720637 2-s2.0-85030720637.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture International 0,591 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
127-138 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964531009519616 |