Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species

Detalhes bibliográficos
Autor(a) principal: Chitiva, Luis Carlos [UNESP]
Data de Publicação: 2023
Outros Autores: Lozano-Puentes, Hair Santiago, Londoño, Ximena, Leão, Tiago F. [UNESP], Cala, Mónica P., Ruiz-Sanchez, Eduardo, Díaz-Ariza, Lucía Ana, Prieto-Rodríguez, Juliet A., Castro-Gamboa, Ian [UNESP], Costa, Geison M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fmolb.2023.1192088
http://hdl.handle.net/11449/247551
Resumo: Bamboo species have traditionally been used as building material and potential source of bioactive substances, as they produce a wide variety of phenolic compounds, including flavonoids and cinnamic acid derivatives that are considered biologically active. However, the effects of growth conditions such as location, altitude, climate, and soil on the metabolome of these species still need to be fully understood. This study aimed to evaluate variations in chemical composition induced by altitudinal gradient (0–3000 m) by utilizing an untargeted metabolomics approach and mapping chemical space using molecular networking analysis. We analyzed 111 samples from 12 bamboo species collected from different altitudinal ranges using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). We used multivariate and univariate statistical analyses to identify the metabolites that showed significant differences in the altitude environments. Additionally, we used the Global Natural Products Social Molecular Networking (GNPS) web platform to perform chemical mapping by comparing the metabolome among the studied species and the reference spectra from its database. The results showed 89 differential metabolites between the altitudinal ranges investigated, wherein high altitude environments significantly increased the profile of flavonoids. While, low altitude environments significantly boosted the profile of cinnamic acid derivatives, particularly caffeoylquinic acids (CQAs). MolNetEnhancer networks confirmed the same differential molecular families already found, revealing metabolic diversity. Overall, this study provides the first report of variations induced by altitude in the chemical profile of bamboo species. The findings may possess fascinating active biological properties, thus offering an alternative use for bamboo.
id UNSP_7ef7237f7e4aa134ad69d2389ed86bf3
oai_identifier_str oai:repositorio.unesp.br:11449/247551
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo speciesaltitudinal variationbamboocinnamic acid derivativesflavonoidsGNPSGuaduanatural productsuntargeted metabolomicsBamboo species have traditionally been used as building material and potential source of bioactive substances, as they produce a wide variety of phenolic compounds, including flavonoids and cinnamic acid derivatives that are considered biologically active. However, the effects of growth conditions such as location, altitude, climate, and soil on the metabolome of these species still need to be fully understood. This study aimed to evaluate variations in chemical composition induced by altitudinal gradient (0–3000 m) by utilizing an untargeted metabolomics approach and mapping chemical space using molecular networking analysis. We analyzed 111 samples from 12 bamboo species collected from different altitudinal ranges using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). We used multivariate and univariate statistical analyses to identify the metabolites that showed significant differences in the altitude environments. Additionally, we used the Global Natural Products Social Molecular Networking (GNPS) web platform to perform chemical mapping by comparing the metabolome among the studied species and the reference spectra from its database. The results showed 89 differential metabolites between the altitudinal ranges investigated, wherein high altitude environments significantly increased the profile of flavonoids. While, low altitude environments significantly boosted the profile of cinnamic acid derivatives, particularly caffeoylquinic acids (CQAs). MolNetEnhancer networks confirmed the same differential molecular families already found, revealing metabolic diversity. Overall, this study provides the first report of variations induced by altitude in the chemical profile of bamboo species. The findings may possess fascinating active biological properties, thus offering an alternative use for bamboo.Department of Chemistry Faculty of Sciences Pontificia Universidad JaverianaInstitute of Chemistry São Paulo State University (UNESP)Department of Biology Faculty of Sciences Pontificia Universidad JaverianaFaculty of Agricultural Sciences Universidad Nacional de ColombiaMetabolomics Core Facility-MetCore Universidad de los AndesDepartment of Botany and Zoology Universidad de GuadalajaraInstitute of Chemistry São Paulo State University (UNESP)Pontificia Universidad JaverianaUniversidade Estadual Paulista (UNESP)Universidad Nacional de ColombiaUniversidad de los AndesUniversidad de GuadalajaraChitiva, Luis Carlos [UNESP]Lozano-Puentes, Hair SantiagoLondoño, XimenaLeão, Tiago F. [UNESP]Cala, Mónica P.Ruiz-Sanchez, EduardoDíaz-Ariza, Lucía AnaPrieto-Rodríguez, Juliet A.Castro-Gamboa, Ian [UNESP]Costa, Geison M.2023-07-29T13:19:10Z2023-07-29T13:19:10Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmolb.2023.1192088Frontiers in Molecular Biosciences, v. 10.2296-889Xhttp://hdl.handle.net/11449/24755110.3389/fmolb.2023.11920882-s2.0-85161420781Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Molecular Biosciencesinfo:eu-repo/semantics/openAccess2023-07-29T13:19:10Zoai:repositorio.unesp.br:11449/247551Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:22:43.483695Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species
title Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species
spellingShingle Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species
Chitiva, Luis Carlos [UNESP]
altitudinal variation
bamboo
cinnamic acid derivatives
flavonoids
GNPS
Guadua
natural products
untargeted metabolomics
title_short Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species
title_full Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species
title_fullStr Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species
title_full_unstemmed Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species
title_sort Untargeted metabolomics approach and molecular networking analysis reveal changes in chemical composition under the influence of altitudinal variation in bamboo species
author Chitiva, Luis Carlos [UNESP]
author_facet Chitiva, Luis Carlos [UNESP]
Lozano-Puentes, Hair Santiago
Londoño, Ximena
Leão, Tiago F. [UNESP]
Cala, Mónica P.
Ruiz-Sanchez, Eduardo
Díaz-Ariza, Lucía Ana
Prieto-Rodríguez, Juliet A.
Castro-Gamboa, Ian [UNESP]
Costa, Geison M.
author_role author
author2 Lozano-Puentes, Hair Santiago
Londoño, Ximena
Leão, Tiago F. [UNESP]
Cala, Mónica P.
Ruiz-Sanchez, Eduardo
Díaz-Ariza, Lucía Ana
Prieto-Rodríguez, Juliet A.
Castro-Gamboa, Ian [UNESP]
Costa, Geison M.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Pontificia Universidad Javeriana
Universidade Estadual Paulista (UNESP)
Universidad Nacional de Colombia
Universidad de los Andes
Universidad de Guadalajara
dc.contributor.author.fl_str_mv Chitiva, Luis Carlos [UNESP]
Lozano-Puentes, Hair Santiago
Londoño, Ximena
Leão, Tiago F. [UNESP]
Cala, Mónica P.
Ruiz-Sanchez, Eduardo
Díaz-Ariza, Lucía Ana
Prieto-Rodríguez, Juliet A.
Castro-Gamboa, Ian [UNESP]
Costa, Geison M.
dc.subject.por.fl_str_mv altitudinal variation
bamboo
cinnamic acid derivatives
flavonoids
GNPS
Guadua
natural products
untargeted metabolomics
topic altitudinal variation
bamboo
cinnamic acid derivatives
flavonoids
GNPS
Guadua
natural products
untargeted metabolomics
description Bamboo species have traditionally been used as building material and potential source of bioactive substances, as they produce a wide variety of phenolic compounds, including flavonoids and cinnamic acid derivatives that are considered biologically active. However, the effects of growth conditions such as location, altitude, climate, and soil on the metabolome of these species still need to be fully understood. This study aimed to evaluate variations in chemical composition induced by altitudinal gradient (0–3000 m) by utilizing an untargeted metabolomics approach and mapping chemical space using molecular networking analysis. We analyzed 111 samples from 12 bamboo species collected from different altitudinal ranges using liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). We used multivariate and univariate statistical analyses to identify the metabolites that showed significant differences in the altitude environments. Additionally, we used the Global Natural Products Social Molecular Networking (GNPS) web platform to perform chemical mapping by comparing the metabolome among the studied species and the reference spectra from its database. The results showed 89 differential metabolites between the altitudinal ranges investigated, wherein high altitude environments significantly increased the profile of flavonoids. While, low altitude environments significantly boosted the profile of cinnamic acid derivatives, particularly caffeoylquinic acids (CQAs). MolNetEnhancer networks confirmed the same differential molecular families already found, revealing metabolic diversity. Overall, this study provides the first report of variations induced by altitude in the chemical profile of bamboo species. The findings may possess fascinating active biological properties, thus offering an alternative use for bamboo.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:19:10Z
2023-07-29T13:19:10Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmolb.2023.1192088
Frontiers in Molecular Biosciences, v. 10.
2296-889X
http://hdl.handle.net/11449/247551
10.3389/fmolb.2023.1192088
2-s2.0-85161420781
url http://dx.doi.org/10.3389/fmolb.2023.1192088
http://hdl.handle.net/11449/247551
identifier_str_mv Frontiers in Molecular Biosciences, v. 10.
2296-889X
10.3389/fmolb.2023.1192088
2-s2.0-85161420781
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Molecular Biosciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128801996013568