Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid

Detalhes bibliográficos
Autor(a) principal: Berton, Mariana P. [UNESP]
Data de Publicação: 2016
Outros Autores: Fonseca, Larissa F. S. [UNESP], Gimenez, Daniela F. J. [UNESP], Utembergue, Bruno L., Cesar, Aline S. M., Coutinho, Luiz L., Lemos, Marcos Vinicius A. de [UNESP], Aboujaoude, Carolyn [UNESP], Pereira, Angelica S. C., O Silva, Rafael M. de [UNESP], Stafuzza, Nedenia B. [UNESP], Feitosa, Fabieli L. B. [UNESP], Chiaia, Hermenegildo L. J. [UNESP], Olivieri, Bianca F. [UNESP], Peripolli, Elisa [UNESP], Tonussi, Rafael L. [UNESP], Gordo, Daniel M. [UNESP], Espigolan, Rafael [UNESP], Ferrinho, Adrielle M., Mueller, Lenise F., Albuquerque, Lucia G. de [UNESP], Oliveira, Henrique N. de [UNESP], Duckett, Susan, Baldi, Femando [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s12864-016-3232-y
http://hdl.handle.net/11449/162192
Resumo: Background: Fatty acid type in beef can be detrimental to human health and has received considerable attention in recent years. The aim of this study was to identify differentially expressed genes in longissimus thoracis muscle of 48 Nellore young bulls with extreme phenotypes for fatty acid composition of intramuscular fat by RNA-seq technique. Results: Differential expression analyses between animals with extreme phenotype for fatty acid composition showed a total of 13 differentially expressed genes for myristic (C14:0), 35 for palmitic (C16:0), 187 for stearic (C18:0), 371 for oleic (C18:1, cis-9), 24 for conjugated linoleic (C18:2 cis-9, trans11, CLA), 89 for linoleic (C18:2 cis-9,12 n6), and 110 genes for a-linolenic (C18:3 n3) fatty acids. For the respective sums of the individual fatty acids, 51 differentially expressed genes for saturated fatty acids (SFA), 336 for monounsaturated (MUFA), 131 for polyunsaturated (PUFA), 92 for PUFA/SFA ratio, 55 for omega 3, 627 for omega 6, and 22 for omega 6/omega 3 ratio were identified. Functional annotation analyses identified several genes associated with fatty acid metabolism, such as those involved in intra and extra-cellular transport of fatty acid synthesis precursors in intramuscular fat of longissimus thoracis muscle. Some of them must be highlighted, such as: ACSM3 and ACSS1 genes, which work as a precursor in fatty acid synthesis; DGAT2 gene that acts in the deposition of saturated fat in the adipose tissue; GPP and LPL genes that support the synthesis of insulin, stimulating both the glucose synthesis and the amino acids entry into the cells; and the BDH1 gene, which is responsible for the synthesis and degradation of ketone bodies used in the synthesis of ATP. Conclusion: Several genes related to lipid metabolism and fatty acid composition were identified. These findings must contribute to the elucidation of the genetic basis to improve Nellore meat quality traits, with emphasis on human health. Additionally, it can also contribute to improve the knowledge of fatty acid biosynthesis and the selection of animals with better nutritional quality.
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spelling Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acidBos indicusHuman healthLipid compositionRNA-seqTranscriptomeBackground: Fatty acid type in beef can be detrimental to human health and has received considerable attention in recent years. The aim of this study was to identify differentially expressed genes in longissimus thoracis muscle of 48 Nellore young bulls with extreme phenotypes for fatty acid composition of intramuscular fat by RNA-seq technique. Results: Differential expression analyses between animals with extreme phenotype for fatty acid composition showed a total of 13 differentially expressed genes for myristic (C14:0), 35 for palmitic (C16:0), 187 for stearic (C18:0), 371 for oleic (C18:1, cis-9), 24 for conjugated linoleic (C18:2 cis-9, trans11, CLA), 89 for linoleic (C18:2 cis-9,12 n6), and 110 genes for a-linolenic (C18:3 n3) fatty acids. For the respective sums of the individual fatty acids, 51 differentially expressed genes for saturated fatty acids (SFA), 336 for monounsaturated (MUFA), 131 for polyunsaturated (PUFA), 92 for PUFA/SFA ratio, 55 for omega 3, 627 for omega 6, and 22 for omega 6/omega 3 ratio were identified. Functional annotation analyses identified several genes associated with fatty acid metabolism, such as those involved in intra and extra-cellular transport of fatty acid synthesis precursors in intramuscular fat of longissimus thoracis muscle. Some of them must be highlighted, such as: ACSM3 and ACSS1 genes, which work as a precursor in fatty acid synthesis; DGAT2 gene that acts in the deposition of saturated fat in the adipose tissue; GPP and LPL genes that support the synthesis of insulin, stimulating both the glucose synthesis and the amino acids entry into the cells; and the BDH1 gene, which is responsible for the synthesis and degradation of ketone bodies used in the synthesis of ATP. Conclusion: Several genes related to lipid metabolism and fatty acid composition were identified. These findings must contribute to the elucidation of the genetic basis to improve Nellore meat quality traits, with emphasis on human health. Additionally, it can also contribute to improve the knowledge of fatty acid biosynthesis and the selection of animals with better nutritional quality.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, BrazilConselho Nacl Desenvolvimento Cient & Tecnol, BR-71605001 Lago Sul, DF, BrazilUniv Sao Paulo, Fac Med Vet & Zootecnia, Dept Nutr & Prod Anim, BR-13635900 Pirassununga, SP, BrazilUniv Sao Paulo, Dept Zootecnia, BR-13418900 Piracicaba, SP, BrazilUniv Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Zootecnia, BR-13635900 Pirassununga, SP, BrazilClemson Univ, Anim & Vet Sci Dept, Clemson, SC 29634 USAUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2011/2141-0FAPESP: 2009/16118-5FAPESP: 2012/15098-3Biomed Central LtdUniversidade Estadual Paulista (Unesp)Conselho Nacl Desenvolvimento Cient & TecnolUniversidade de São Paulo (USP)Clemson UnivBerton, Mariana P. [UNESP]Fonseca, Larissa F. S. [UNESP]Gimenez, Daniela F. J. [UNESP]Utembergue, Bruno L.Cesar, Aline S. M.Coutinho, Luiz L.Lemos, Marcos Vinicius A. de [UNESP]Aboujaoude, Carolyn [UNESP]Pereira, Angelica S. C.O Silva, Rafael M. de [UNESP]Stafuzza, Nedenia B. [UNESP]Feitosa, Fabieli L. B. [UNESP]Chiaia, Hermenegildo L. J. [UNESP]Olivieri, Bianca F. [UNESP]Peripolli, Elisa [UNESP]Tonussi, Rafael L. [UNESP]Gordo, Daniel M. [UNESP]Espigolan, Rafael [UNESP]Ferrinho, Adrielle M.Mueller, Lenise F.Albuquerque, Lucia G. de [UNESP]Oliveira, Henrique N. de [UNESP]Duckett, SusanBaldi, Femando [UNESP]2018-11-26T17:12:11Z2018-11-26T17:12:11Z2016-11-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://dx.doi.org/10.1186/s12864-016-3232-yBmc Genomics. London: Biomed Central Ltd, v. 17, 16 p., 2016.1471-2164http://hdl.handle.net/11449/16219210.1186/s12864-016-3232-yWOS:000388773400002WOS000388773400002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Genomics2,110info:eu-repo/semantics/openAccess2024-06-07T18:39:52Zoai:repositorio.unesp.br:11449/162192Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:10:16.110187Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
title Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
spellingShingle Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
Berton, Mariana P. [UNESP]
Bos indicus
Human health
Lipid composition
RNA-seq
Transcriptome
title_short Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
title_full Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
title_fullStr Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
title_full_unstemmed Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
title_sort Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
author Berton, Mariana P. [UNESP]
author_facet Berton, Mariana P. [UNESP]
Fonseca, Larissa F. S. [UNESP]
Gimenez, Daniela F. J. [UNESP]
Utembergue, Bruno L.
Cesar, Aline S. M.
Coutinho, Luiz L.
Lemos, Marcos Vinicius A. de [UNESP]
Aboujaoude, Carolyn [UNESP]
Pereira, Angelica S. C.
O Silva, Rafael M. de [UNESP]
Stafuzza, Nedenia B. [UNESP]
Feitosa, Fabieli L. B. [UNESP]
Chiaia, Hermenegildo L. J. [UNESP]
Olivieri, Bianca F. [UNESP]
Peripolli, Elisa [UNESP]
Tonussi, Rafael L. [UNESP]
Gordo, Daniel M. [UNESP]
Espigolan, Rafael [UNESP]
Ferrinho, Adrielle M.
Mueller, Lenise F.
Albuquerque, Lucia G. de [UNESP]
Oliveira, Henrique N. de [UNESP]
Duckett, Susan
Baldi, Femando [UNESP]
author_role author
author2 Fonseca, Larissa F. S. [UNESP]
Gimenez, Daniela F. J. [UNESP]
Utembergue, Bruno L.
Cesar, Aline S. M.
Coutinho, Luiz L.
Lemos, Marcos Vinicius A. de [UNESP]
Aboujaoude, Carolyn [UNESP]
Pereira, Angelica S. C.
O Silva, Rafael M. de [UNESP]
Stafuzza, Nedenia B. [UNESP]
Feitosa, Fabieli L. B. [UNESP]
Chiaia, Hermenegildo L. J. [UNESP]
Olivieri, Bianca F. [UNESP]
Peripolli, Elisa [UNESP]
Tonussi, Rafael L. [UNESP]
Gordo, Daniel M. [UNESP]
Espigolan, Rafael [UNESP]
Ferrinho, Adrielle M.
Mueller, Lenise F.
Albuquerque, Lucia G. de [UNESP]
Oliveira, Henrique N. de [UNESP]
Duckett, Susan
Baldi, Femando [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Conselho Nacl Desenvolvimento Cient & Tecnol
Universidade de São Paulo (USP)
Clemson Univ
dc.contributor.author.fl_str_mv Berton, Mariana P. [UNESP]
Fonseca, Larissa F. S. [UNESP]
Gimenez, Daniela F. J. [UNESP]
Utembergue, Bruno L.
Cesar, Aline S. M.
Coutinho, Luiz L.
Lemos, Marcos Vinicius A. de [UNESP]
Aboujaoude, Carolyn [UNESP]
Pereira, Angelica S. C.
O Silva, Rafael M. de [UNESP]
Stafuzza, Nedenia B. [UNESP]
Feitosa, Fabieli L. B. [UNESP]
Chiaia, Hermenegildo L. J. [UNESP]
Olivieri, Bianca F. [UNESP]
Peripolli, Elisa [UNESP]
Tonussi, Rafael L. [UNESP]
Gordo, Daniel M. [UNESP]
Espigolan, Rafael [UNESP]
Ferrinho, Adrielle M.
Mueller, Lenise F.
Albuquerque, Lucia G. de [UNESP]
Oliveira, Henrique N. de [UNESP]
Duckett, Susan
Baldi, Femando [UNESP]
dc.subject.por.fl_str_mv Bos indicus
Human health
Lipid composition
RNA-seq
Transcriptome
topic Bos indicus
Human health
Lipid composition
RNA-seq
Transcriptome
description Background: Fatty acid type in beef can be detrimental to human health and has received considerable attention in recent years. The aim of this study was to identify differentially expressed genes in longissimus thoracis muscle of 48 Nellore young bulls with extreme phenotypes for fatty acid composition of intramuscular fat by RNA-seq technique. Results: Differential expression analyses between animals with extreme phenotype for fatty acid composition showed a total of 13 differentially expressed genes for myristic (C14:0), 35 for palmitic (C16:0), 187 for stearic (C18:0), 371 for oleic (C18:1, cis-9), 24 for conjugated linoleic (C18:2 cis-9, trans11, CLA), 89 for linoleic (C18:2 cis-9,12 n6), and 110 genes for a-linolenic (C18:3 n3) fatty acids. For the respective sums of the individual fatty acids, 51 differentially expressed genes for saturated fatty acids (SFA), 336 for monounsaturated (MUFA), 131 for polyunsaturated (PUFA), 92 for PUFA/SFA ratio, 55 for omega 3, 627 for omega 6, and 22 for omega 6/omega 3 ratio were identified. Functional annotation analyses identified several genes associated with fatty acid metabolism, such as those involved in intra and extra-cellular transport of fatty acid synthesis precursors in intramuscular fat of longissimus thoracis muscle. Some of them must be highlighted, such as: ACSM3 and ACSS1 genes, which work as a precursor in fatty acid synthesis; DGAT2 gene that acts in the deposition of saturated fat in the adipose tissue; GPP and LPL genes that support the synthesis of insulin, stimulating both the glucose synthesis and the amino acids entry into the cells; and the BDH1 gene, which is responsible for the synthesis and degradation of ketone bodies used in the synthesis of ATP. Conclusion: Several genes related to lipid metabolism and fatty acid composition were identified. These findings must contribute to the elucidation of the genetic basis to improve Nellore meat quality traits, with emphasis on human health. Additionally, it can also contribute to improve the knowledge of fatty acid biosynthesis and the selection of animals with better nutritional quality.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-25
2018-11-26T17:12:11Z
2018-11-26T17:12:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12864-016-3232-y
Bmc Genomics. London: Biomed Central Ltd, v. 17, 16 p., 2016.
1471-2164
http://hdl.handle.net/11449/162192
10.1186/s12864-016-3232-y
WOS:000388773400002
WOS000388773400002.pdf
url http://dx.doi.org/10.1186/s12864-016-3232-y
http://hdl.handle.net/11449/162192
identifier_str_mv Bmc Genomics. London: Biomed Central Ltd, v. 17, 16 p., 2016.
1471-2164
10.1186/s12864-016-3232-y
WOS:000388773400002
WOS000388773400002.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bmc Genomics
2,110
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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