Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12864-016-3232-y http://hdl.handle.net/11449/162192 |
Resumo: | Background: Fatty acid type in beef can be detrimental to human health and has received considerable attention in recent years. The aim of this study was to identify differentially expressed genes in longissimus thoracis muscle of 48 Nellore young bulls with extreme phenotypes for fatty acid composition of intramuscular fat by RNA-seq technique. Results: Differential expression analyses between animals with extreme phenotype for fatty acid composition showed a total of 13 differentially expressed genes for myristic (C14:0), 35 for palmitic (C16:0), 187 for stearic (C18:0), 371 for oleic (C18:1, cis-9), 24 for conjugated linoleic (C18:2 cis-9, trans11, CLA), 89 for linoleic (C18:2 cis-9,12 n6), and 110 genes for a-linolenic (C18:3 n3) fatty acids. For the respective sums of the individual fatty acids, 51 differentially expressed genes for saturated fatty acids (SFA), 336 for monounsaturated (MUFA), 131 for polyunsaturated (PUFA), 92 for PUFA/SFA ratio, 55 for omega 3, 627 for omega 6, and 22 for omega 6/omega 3 ratio were identified. Functional annotation analyses identified several genes associated with fatty acid metabolism, such as those involved in intra and extra-cellular transport of fatty acid synthesis precursors in intramuscular fat of longissimus thoracis muscle. Some of them must be highlighted, such as: ACSM3 and ACSS1 genes, which work as a precursor in fatty acid synthesis; DGAT2 gene that acts in the deposition of saturated fat in the adipose tissue; GPP and LPL genes that support the synthesis of insulin, stimulating both the glucose synthesis and the amino acids entry into the cells; and the BDH1 gene, which is responsible for the synthesis and degradation of ketone bodies used in the synthesis of ATP. Conclusion: Several genes related to lipid metabolism and fatty acid composition were identified. These findings must contribute to the elucidation of the genetic basis to improve Nellore meat quality traits, with emphasis on human health. Additionally, it can also contribute to improve the knowledge of fatty acid biosynthesis and the selection of animals with better nutritional quality. |
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Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acidBos indicusHuman healthLipid compositionRNA-seqTranscriptomeBackground: Fatty acid type in beef can be detrimental to human health and has received considerable attention in recent years. The aim of this study was to identify differentially expressed genes in longissimus thoracis muscle of 48 Nellore young bulls with extreme phenotypes for fatty acid composition of intramuscular fat by RNA-seq technique. Results: Differential expression analyses between animals with extreme phenotype for fatty acid composition showed a total of 13 differentially expressed genes for myristic (C14:0), 35 for palmitic (C16:0), 187 for stearic (C18:0), 371 for oleic (C18:1, cis-9), 24 for conjugated linoleic (C18:2 cis-9, trans11, CLA), 89 for linoleic (C18:2 cis-9,12 n6), and 110 genes for a-linolenic (C18:3 n3) fatty acids. For the respective sums of the individual fatty acids, 51 differentially expressed genes for saturated fatty acids (SFA), 336 for monounsaturated (MUFA), 131 for polyunsaturated (PUFA), 92 for PUFA/SFA ratio, 55 for omega 3, 627 for omega 6, and 22 for omega 6/omega 3 ratio were identified. Functional annotation analyses identified several genes associated with fatty acid metabolism, such as those involved in intra and extra-cellular transport of fatty acid synthesis precursors in intramuscular fat of longissimus thoracis muscle. Some of them must be highlighted, such as: ACSM3 and ACSS1 genes, which work as a precursor in fatty acid synthesis; DGAT2 gene that acts in the deposition of saturated fat in the adipose tissue; GPP and LPL genes that support the synthesis of insulin, stimulating both the glucose synthesis and the amino acids entry into the cells; and the BDH1 gene, which is responsible for the synthesis and degradation of ketone bodies used in the synthesis of ATP. Conclusion: Several genes related to lipid metabolism and fatty acid composition were identified. These findings must contribute to the elucidation of the genetic basis to improve Nellore meat quality traits, with emphasis on human health. Additionally, it can also contribute to improve the knowledge of fatty acid biosynthesis and the selection of animals with better nutritional quality.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, BrazilConselho Nacl Desenvolvimento Cient & Tecnol, BR-71605001 Lago Sul, DF, BrazilUniv Sao Paulo, Fac Med Vet & Zootecnia, Dept Nutr & Prod Anim, BR-13635900 Pirassununga, SP, BrazilUniv Sao Paulo, Dept Zootecnia, BR-13418900 Piracicaba, SP, BrazilUniv Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Zootecnia, BR-13635900 Pirassununga, SP, BrazilClemson Univ, Anim & Vet Sci Dept, Clemson, SC 29634 USAUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2011/2141-0FAPESP: 2009/16118-5FAPESP: 2012/15098-3Biomed Central LtdUniversidade Estadual Paulista (Unesp)Conselho Nacl Desenvolvimento Cient & TecnolUniversidade de São Paulo (USP)Clemson UnivBerton, Mariana P. [UNESP]Fonseca, Larissa F. S. [UNESP]Gimenez, Daniela F. J. [UNESP]Utembergue, Bruno L.Cesar, Aline S. M.Coutinho, Luiz L.Lemos, Marcos Vinicius A. de [UNESP]Aboujaoude, Carolyn [UNESP]Pereira, Angelica S. C.O Silva, Rafael M. de [UNESP]Stafuzza, Nedenia B. [UNESP]Feitosa, Fabieli L. B. [UNESP]Chiaia, Hermenegildo L. J. [UNESP]Olivieri, Bianca F. [UNESP]Peripolli, Elisa [UNESP]Tonussi, Rafael L. [UNESP]Gordo, Daniel M. [UNESP]Espigolan, Rafael [UNESP]Ferrinho, Adrielle M.Mueller, Lenise F.Albuquerque, Lucia G. de [UNESP]Oliveira, Henrique N. de [UNESP]Duckett, SusanBaldi, Femando [UNESP]2018-11-26T17:12:11Z2018-11-26T17:12:11Z2016-11-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://dx.doi.org/10.1186/s12864-016-3232-yBmc Genomics. London: Biomed Central Ltd, v. 17, 16 p., 2016.1471-2164http://hdl.handle.net/11449/16219210.1186/s12864-016-3232-yWOS:000388773400002WOS000388773400002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Genomics2,110info:eu-repo/semantics/openAccess2024-06-07T18:39:52Zoai:repositorio.unesp.br:11449/162192Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:10:16.110187Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid |
title |
Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid |
spellingShingle |
Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid Berton, Mariana P. [UNESP] Bos indicus Human health Lipid composition RNA-seq Transcriptome |
title_short |
Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid |
title_full |
Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid |
title_fullStr |
Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid |
title_full_unstemmed |
Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid |
title_sort |
Gene expression profile of intramuscular muscle in Nellore cattle with extreme values of fatty acid |
author |
Berton, Mariana P. [UNESP] |
author_facet |
Berton, Mariana P. [UNESP] Fonseca, Larissa F. S. [UNESP] Gimenez, Daniela F. J. [UNESP] Utembergue, Bruno L. Cesar, Aline S. M. Coutinho, Luiz L. Lemos, Marcos Vinicius A. de [UNESP] Aboujaoude, Carolyn [UNESP] Pereira, Angelica S. C. O Silva, Rafael M. de [UNESP] Stafuzza, Nedenia B. [UNESP] Feitosa, Fabieli L. B. [UNESP] Chiaia, Hermenegildo L. J. [UNESP] Olivieri, Bianca F. [UNESP] Peripolli, Elisa [UNESP] Tonussi, Rafael L. [UNESP] Gordo, Daniel M. [UNESP] Espigolan, Rafael [UNESP] Ferrinho, Adrielle M. Mueller, Lenise F. Albuquerque, Lucia G. de [UNESP] Oliveira, Henrique N. de [UNESP] Duckett, Susan Baldi, Femando [UNESP] |
author_role |
author |
author2 |
Fonseca, Larissa F. S. [UNESP] Gimenez, Daniela F. J. [UNESP] Utembergue, Bruno L. Cesar, Aline S. M. Coutinho, Luiz L. Lemos, Marcos Vinicius A. de [UNESP] Aboujaoude, Carolyn [UNESP] Pereira, Angelica S. C. O Silva, Rafael M. de [UNESP] Stafuzza, Nedenia B. [UNESP] Feitosa, Fabieli L. B. [UNESP] Chiaia, Hermenegildo L. J. [UNESP] Olivieri, Bianca F. [UNESP] Peripolli, Elisa [UNESP] Tonussi, Rafael L. [UNESP] Gordo, Daniel M. [UNESP] Espigolan, Rafael [UNESP] Ferrinho, Adrielle M. Mueller, Lenise F. Albuquerque, Lucia G. de [UNESP] Oliveira, Henrique N. de [UNESP] Duckett, Susan Baldi, Femando [UNESP] |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Conselho Nacl Desenvolvimento Cient & Tecnol Universidade de São Paulo (USP) Clemson Univ |
dc.contributor.author.fl_str_mv |
Berton, Mariana P. [UNESP] Fonseca, Larissa F. S. [UNESP] Gimenez, Daniela F. J. [UNESP] Utembergue, Bruno L. Cesar, Aline S. M. Coutinho, Luiz L. Lemos, Marcos Vinicius A. de [UNESP] Aboujaoude, Carolyn [UNESP] Pereira, Angelica S. C. O Silva, Rafael M. de [UNESP] Stafuzza, Nedenia B. [UNESP] Feitosa, Fabieli L. B. [UNESP] Chiaia, Hermenegildo L. J. [UNESP] Olivieri, Bianca F. [UNESP] Peripolli, Elisa [UNESP] Tonussi, Rafael L. [UNESP] Gordo, Daniel M. [UNESP] Espigolan, Rafael [UNESP] Ferrinho, Adrielle M. Mueller, Lenise F. Albuquerque, Lucia G. de [UNESP] Oliveira, Henrique N. de [UNESP] Duckett, Susan Baldi, Femando [UNESP] |
dc.subject.por.fl_str_mv |
Bos indicus Human health Lipid composition RNA-seq Transcriptome |
topic |
Bos indicus Human health Lipid composition RNA-seq Transcriptome |
description |
Background: Fatty acid type in beef can be detrimental to human health and has received considerable attention in recent years. The aim of this study was to identify differentially expressed genes in longissimus thoracis muscle of 48 Nellore young bulls with extreme phenotypes for fatty acid composition of intramuscular fat by RNA-seq technique. Results: Differential expression analyses between animals with extreme phenotype for fatty acid composition showed a total of 13 differentially expressed genes for myristic (C14:0), 35 for palmitic (C16:0), 187 for stearic (C18:0), 371 for oleic (C18:1, cis-9), 24 for conjugated linoleic (C18:2 cis-9, trans11, CLA), 89 for linoleic (C18:2 cis-9,12 n6), and 110 genes for a-linolenic (C18:3 n3) fatty acids. For the respective sums of the individual fatty acids, 51 differentially expressed genes for saturated fatty acids (SFA), 336 for monounsaturated (MUFA), 131 for polyunsaturated (PUFA), 92 for PUFA/SFA ratio, 55 for omega 3, 627 for omega 6, and 22 for omega 6/omega 3 ratio were identified. Functional annotation analyses identified several genes associated with fatty acid metabolism, such as those involved in intra and extra-cellular transport of fatty acid synthesis precursors in intramuscular fat of longissimus thoracis muscle. Some of them must be highlighted, such as: ACSM3 and ACSS1 genes, which work as a precursor in fatty acid synthesis; DGAT2 gene that acts in the deposition of saturated fat in the adipose tissue; GPP and LPL genes that support the synthesis of insulin, stimulating both the glucose synthesis and the amino acids entry into the cells; and the BDH1 gene, which is responsible for the synthesis and degradation of ketone bodies used in the synthesis of ATP. Conclusion: Several genes related to lipid metabolism and fatty acid composition were identified. These findings must contribute to the elucidation of the genetic basis to improve Nellore meat quality traits, with emphasis on human health. Additionally, it can also contribute to improve the knowledge of fatty acid biosynthesis and the selection of animals with better nutritional quality. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-25 2018-11-26T17:12:11Z 2018-11-26T17:12:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12864-016-3232-y Bmc Genomics. London: Biomed Central Ltd, v. 17, 16 p., 2016. 1471-2164 http://hdl.handle.net/11449/162192 10.1186/s12864-016-3232-y WOS:000388773400002 WOS000388773400002.pdf |
url |
http://dx.doi.org/10.1186/s12864-016-3232-y http://hdl.handle.net/11449/162192 |
identifier_str_mv |
Bmc Genomics. London: Biomed Central Ltd, v. 17, 16 p., 2016. 1471-2164 10.1186/s12864-016-3232-y WOS:000388773400002 WOS000388773400002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Genomics 2,110 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128328018690048 |