Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats
Autor(a) principal: | |
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Data de Publicação: | 1994 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/223978 |
Resumo: | Fencamfamine (FCF) is a psychostimulant drug classified as an indirect dopamine agonist. In the present study we evaluated the daily variation in plasma FCF concentration and in striatal dopamine receptors. Adult male Wistar rats (250-300 g) maintained on a 12-h light/12-h dark cycle (lights on at 07:00 h) were used. Rats received FCF (10.0 mg/kg, ip) at 09:00, 15:00, 21:00 or 03:00 h and blood samples were collected 30 (N = 6) or 60 (N = 6) min after the injections. Plasma FCF was measured by gas chromatography using an electron capture detector. Two-way ANOVA showed significant differences in FCF concentration when blood samples were collected 30 min after the injection, and the highest value was obtained following injection at 21:00 h. Moreover, at 15:00, 21:00 and 03:00 h, plasma FCF levels were significantly lower 60 min after injection when compared to the 30-min interval. Two other groups of rats (N = 6) were decapitated at 09:00 or 21:00 h and the striata were dissected for the binding assays. The Bmax for [3H]-spiroperidol binding to striatal membranes was higher at 21:00 h, without changes in affinity constant (Kd). In conclusion, plasma FCF levels and dopamine receptors undergo daily variation, a phenomenon that should be considered to explain the circadian time-dependent effects of FCF. |
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Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in ratsFencamfamine (FCF) is a psychostimulant drug classified as an indirect dopamine agonist. In the present study we evaluated the daily variation in plasma FCF concentration and in striatal dopamine receptors. Adult male Wistar rats (250-300 g) maintained on a 12-h light/12-h dark cycle (lights on at 07:00 h) were used. Rats received FCF (10.0 mg/kg, ip) at 09:00, 15:00, 21:00 or 03:00 h and blood samples were collected 30 (N = 6) or 60 (N = 6) min after the injections. Plasma FCF was measured by gas chromatography using an electron capture detector. Two-way ANOVA showed significant differences in FCF concentration when blood samples were collected 30 min after the injection, and the highest value was obtained following injection at 21:00 h. Moreover, at 15:00, 21:00 and 03:00 h, plasma FCF levels were significantly lower 60 min after injection when compared to the 30-min interval. Two other groups of rats (N = 6) were decapitated at 09:00 or 21:00 h and the striata were dissected for the binding assays. The Bmax for [3H]-spiroperidol binding to striatal membranes was higher at 21:00 h, without changes in affinity constant (Kd). In conclusion, plasma FCF levels and dopamine receptors undergo daily variation, a phenomenon that should be considered to explain the circadian time-dependent effects of FCF.DPANT Faculdade de Ciencias Farmaceuticas UNESP-Araraquara, Rodovia Araraquara-Jau Km 01, 14801-902 Araraquara, SPDPANT Faculdade de Ciencias Farmaceuticas UNESP-Araraquara, Rodovia Araraquara-Jau Km 01, 14801-902 Araraquara, SPUniversidade Estadual Paulista (UNESP)Planeta, C. S. [UNESP]DeLucia, R. [UNESP]Aizenstein, M. L. [UNESP]Oliveira, G. H. [UNESP]2022-04-28T19:54:01Z2022-04-28T19:54:01Z1994-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article737-741Brazilian Journal of Medical and Biological Research, v. 27, n. 3, p. 737-741, 1994.0100-879Xhttp://hdl.handle.net/11449/2239782-s2.0-0028266031Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccess2022-04-28T19:54:01Zoai:repositorio.unesp.br:11449/223978Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:54:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats |
title |
Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats |
spellingShingle |
Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats Planeta, C. S. [UNESP] |
title_short |
Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats |
title_full |
Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats |
title_fullStr |
Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats |
title_full_unstemmed |
Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats |
title_sort |
Daily variation in plasma concentration of fencamfamine and striatal dopamine receptors in rats |
author |
Planeta, C. S. [UNESP] |
author_facet |
Planeta, C. S. [UNESP] DeLucia, R. [UNESP] Aizenstein, M. L. [UNESP] Oliveira, G. H. [UNESP] |
author_role |
author |
author2 |
DeLucia, R. [UNESP] Aizenstein, M. L. [UNESP] Oliveira, G. H. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Planeta, C. S. [UNESP] DeLucia, R. [UNESP] Aizenstein, M. L. [UNESP] Oliveira, G. H. [UNESP] |
description |
Fencamfamine (FCF) is a psychostimulant drug classified as an indirect dopamine agonist. In the present study we evaluated the daily variation in plasma FCF concentration and in striatal dopamine receptors. Adult male Wistar rats (250-300 g) maintained on a 12-h light/12-h dark cycle (lights on at 07:00 h) were used. Rats received FCF (10.0 mg/kg, ip) at 09:00, 15:00, 21:00 or 03:00 h and blood samples were collected 30 (N = 6) or 60 (N = 6) min after the injections. Plasma FCF was measured by gas chromatography using an electron capture detector. Two-way ANOVA showed significant differences in FCF concentration when blood samples were collected 30 min after the injection, and the highest value was obtained following injection at 21:00 h. Moreover, at 15:00, 21:00 and 03:00 h, plasma FCF levels were significantly lower 60 min after injection when compared to the 30-min interval. Two other groups of rats (N = 6) were decapitated at 09:00 or 21:00 h and the striata were dissected for the binding assays. The Bmax for [3H]-spiroperidol binding to striatal membranes was higher at 21:00 h, without changes in affinity constant (Kd). In conclusion, plasma FCF levels and dopamine receptors undergo daily variation, a phenomenon that should be considered to explain the circadian time-dependent effects of FCF. |
publishDate |
1994 |
dc.date.none.fl_str_mv |
1994-01-01 2022-04-28T19:54:01Z 2022-04-28T19:54:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Medical and Biological Research, v. 27, n. 3, p. 737-741, 1994. 0100-879X http://hdl.handle.net/11449/223978 2-s2.0-0028266031 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research, v. 27, n. 3, p. 737-741, 1994. 0100-879X 2-s2.0-0028266031 |
url |
http://hdl.handle.net/11449/223978 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
737-741 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965302403891200 |