Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-020-68949-5 http://hdl.handle.net/11449/195562 |
Resumo: | Capsicum annuum is one of the most important horticultural crops worldwide. Anthracnose disease (Colletotrichum spp.) is a major constraint for chili production, causing substantial losses. Capsidiol is a sesquiterpene phytoalexin present in pepper fruits that can enhance plant resistance. The genetic mechanisms involved in capisidiol biosynthesis are still poorly understood. In this study, a 3 ' RNA sequencing approach was used to develop the transcriptional profile dataset of C. annuum genes in unripe (UF) and ripe fruits (RF) in response to C. scovillei infection. Results showed 4,845 upregulated and 4,720 downregulated genes in UF, and 2,560 upregulated and 1,762 downregulated genes in RF under fungus inoculation. Four capsidiol-related genes were selected for RT-qPCR analysis, two 5-epi-aristolochene synthase (CA12g05030, CA02g09520) and two 5-epi-aristolochene-1,3-dihydroxylase genes (CA12g05070, CA01g05990). CA12g05030 and CA01g05990 genes showed an early response to fungus infection in RF (24 h post-inoculation-HPI), being 68-fold and 53-fold more expressed at 96 HPI, respectively. In UF, all genes showed a late response, especially CA12g05030, which was 700-fold more expressed at 96 HPI compared to control plants. We are proving here the first high-throughput expression dataset of pepper fruits in response to anthracnose disease in order to contribute for future pepper breeding programs. |
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Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stagesCapsicum annuum is one of the most important horticultural crops worldwide. Anthracnose disease (Colletotrichum spp.) is a major constraint for chili production, causing substantial losses. Capsidiol is a sesquiterpene phytoalexin present in pepper fruits that can enhance plant resistance. The genetic mechanisms involved in capisidiol biosynthesis are still poorly understood. In this study, a 3 ' RNA sequencing approach was used to develop the transcriptional profile dataset of C. annuum genes in unripe (UF) and ripe fruits (RF) in response to C. scovillei infection. Results showed 4,845 upregulated and 4,720 downregulated genes in UF, and 2,560 upregulated and 1,762 downregulated genes in RF under fungus inoculation. Four capsidiol-related genes were selected for RT-qPCR analysis, two 5-epi-aristolochene synthase (CA12g05030, CA02g09520) and two 5-epi-aristolochene-1,3-dihydroxylase genes (CA12g05070, CA01g05990). CA12g05030 and CA01g05990 genes showed an early response to fungus infection in RF (24 h post-inoculation-HPI), being 68-fold and 53-fold more expressed at 96 HPI, respectively. In UF, all genes showed a late response, especially CA12g05030, which was 700-fold more expressed at 96 HPI compared to control plants. We are proving here the first high-throughput expression dataset of pepper fruits in response to anthracnose disease in order to contribute for future pepper breeding programs.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Londrina, Programa Posgrad Agron, Lab Ecofisiol & Biotecnol Agr, Londrina, Parana, BrazilBoyce Thompson Inst Plant Res, Ithaca, NY USAUniv Estadual Paulista, Inst Biociencias, Rio Claro, BrazilEmpresa Brasileira Pesquisa Agr, Brasilia, DF, BrazilUniv Estadual Londrina, Lab Citogenet & Diversidade Vegetal, Londrina, Parana, BrazilUniv Estadual Norte Fluminense, Genet & Melhoramento Plantas, Campos Goytacazes, Goytacazes, BrazilUniv Estadual Paulista, Inst Biociencias, Rio Claro, BrazilFAPESP: 2017/01455-2Nature Publishing GroupUniversidade Estadual de Londrina (UEL)Boyce Thompson Inst Plant ResUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Univ Estadual Norte FluminenseBaba, Viviane Y.Powell, Adrian F.Ivamoto-Suzuki, Suzana T. [UNESP]Pereira, Luiz F. P.Vanzela, Andre L. L.Giacomin, Renata M.Strickler, Susan R.Mueller, Lukas A.Rodrigues, RosanaGoncalves, Leandro S. A.2020-12-10T17:38:46Z2020-12-10T17:38:46Z2020-07-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.1038/s41598-020-68949-5Scientific Reports. London: Nature Publishing Group, v. 10, n. 1, 14 p., 2020.2045-2322http://hdl.handle.net/11449/19556210.1038/s41598-020-68949-5WOS:000555518700019Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-23T09:41:44Zoai:repositorio.unesp.br:11449/195562Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T09:41:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages |
title |
Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages |
spellingShingle |
Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages Baba, Viviane Y. |
title_short |
Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages |
title_full |
Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages |
title_fullStr |
Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages |
title_full_unstemmed |
Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages |
title_sort |
Capsidiol-related genes are highly expressed in response to Colletotrichum scovillei during Capsicum annuum fruit development stages |
author |
Baba, Viviane Y. |
author_facet |
Baba, Viviane Y. Powell, Adrian F. Ivamoto-Suzuki, Suzana T. [UNESP] Pereira, Luiz F. P. Vanzela, Andre L. L. Giacomin, Renata M. Strickler, Susan R. Mueller, Lukas A. Rodrigues, Rosana Goncalves, Leandro S. A. |
author_role |
author |
author2 |
Powell, Adrian F. Ivamoto-Suzuki, Suzana T. [UNESP] Pereira, Luiz F. P. Vanzela, Andre L. L. Giacomin, Renata M. Strickler, Susan R. Mueller, Lukas A. Rodrigues, Rosana Goncalves, Leandro S. A. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Boyce Thompson Inst Plant Res Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Univ Estadual Norte Fluminense |
dc.contributor.author.fl_str_mv |
Baba, Viviane Y. Powell, Adrian F. Ivamoto-Suzuki, Suzana T. [UNESP] Pereira, Luiz F. P. Vanzela, Andre L. L. Giacomin, Renata M. Strickler, Susan R. Mueller, Lukas A. Rodrigues, Rosana Goncalves, Leandro S. A. |
description |
Capsicum annuum is one of the most important horticultural crops worldwide. Anthracnose disease (Colletotrichum spp.) is a major constraint for chili production, causing substantial losses. Capsidiol is a sesquiterpene phytoalexin present in pepper fruits that can enhance plant resistance. The genetic mechanisms involved in capisidiol biosynthesis are still poorly understood. In this study, a 3 ' RNA sequencing approach was used to develop the transcriptional profile dataset of C. annuum genes in unripe (UF) and ripe fruits (RF) in response to C. scovillei infection. Results showed 4,845 upregulated and 4,720 downregulated genes in UF, and 2,560 upregulated and 1,762 downregulated genes in RF under fungus inoculation. Four capsidiol-related genes were selected for RT-qPCR analysis, two 5-epi-aristolochene synthase (CA12g05030, CA02g09520) and two 5-epi-aristolochene-1,3-dihydroxylase genes (CA12g05070, CA01g05990). CA12g05030 and CA01g05990 genes showed an early response to fungus infection in RF (24 h post-inoculation-HPI), being 68-fold and 53-fold more expressed at 96 HPI, respectively. In UF, all genes showed a late response, especially CA12g05030, which was 700-fold more expressed at 96 HPI compared to control plants. We are proving here the first high-throughput expression dataset of pepper fruits in response to anthracnose disease in order to contribute for future pepper breeding programs. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T17:38:46Z 2020-12-10T17:38:46Z 2020-07-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-020-68949-5 Scientific Reports. London: Nature Publishing Group, v. 10, n. 1, 14 p., 2020. 2045-2322 http://hdl.handle.net/11449/195562 10.1038/s41598-020-68949-5 WOS:000555518700019 |
url |
http://dx.doi.org/10.1038/s41598-020-68949-5 http://hdl.handle.net/11449/195562 |
identifier_str_mv |
Scientific Reports. London: Nature Publishing Group, v. 10, n. 1, 14 p., 2020. 2045-2322 10.1038/s41598-020-68949-5 WOS:000555518700019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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