Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0222626 http://hdl.handle.net/11449/199442 |
Resumo: | Aeromonas bacteria can cause an infection characterized by septicemia and is one of the most common pathogens in tropical fish. This disease is responsible for high morbidity and mortality rates, causing considerable losses in aquaculture. Thus, the understanding of its pathophysiology is crucial to develop control strategies of this bacterial infection in farmed fish. This study aimed to characterize early pathological aspects of acute sepsis in pacu (Piaractus mesopotamicus) experimentally infected with Aeromonas hydrophila. A total of 160 juvenile pacus were inoculated intraperitoneally with A. hydrophila (1.78 x 109 CFU/mL) and at 0 (control), 1, 3, 6, and 9 hours post-inoculation (hpi), animals were anesthetized and samples were collected for microbiological, light microscopy and transmission electron microscopy (TEM) analyzes. The results showed the occurrence of hemodynamic alterations, such as hemorrhage and congestion, which were observed mainly after 6 and 9 hpi. It was possible to re-isolate Aeromonas at all sampling times except in control group. However, just after 9 hpi it was possible to find the bacteria in all fish and tissues. Light microscopy analyses revealed a degenerative process, necrosis and vascular damage mainly at 6 and 9 hpi. According to the ultrastructural examination, areas of cellular death were identified in all examined tissues, especially at 6 and 9 hpi. However, the most severe, related to necrosis, were observed after 6 and 9 hpi. The findings suggested that this bacterium spreads in the first hpi through the fish organs, mainly affecting spleen, liver and kidney, causing irreversible lesions at the molecular level. |
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Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicusAeromonas bacteria can cause an infection characterized by septicemia and is one of the most common pathogens in tropical fish. This disease is responsible for high morbidity and mortality rates, causing considerable losses in aquaculture. Thus, the understanding of its pathophysiology is crucial to develop control strategies of this bacterial infection in farmed fish. This study aimed to characterize early pathological aspects of acute sepsis in pacu (Piaractus mesopotamicus) experimentally infected with Aeromonas hydrophila. A total of 160 juvenile pacus were inoculated intraperitoneally with A. hydrophila (1.78 x 109 CFU/mL) and at 0 (control), 1, 3, 6, and 9 hours post-inoculation (hpi), animals were anesthetized and samples were collected for microbiological, light microscopy and transmission electron microscopy (TEM) analyzes. The results showed the occurrence of hemodynamic alterations, such as hemorrhage and congestion, which were observed mainly after 6 and 9 hpi. It was possible to re-isolate Aeromonas at all sampling times except in control group. However, just after 9 hpi it was possible to find the bacteria in all fish and tissues. Light microscopy analyses revealed a degenerative process, necrosis and vascular damage mainly at 6 and 9 hpi. According to the ultrastructural examination, areas of cellular death were identified in all examined tissues, especially at 6 and 9 hpi. However, the most severe, related to necrosis, were observed after 6 and 9 hpi. The findings suggested that this bacterium spreads in the first hpi through the fish organs, mainly affecting spleen, liver and kidney, causing irreversible lesions at the molecular level.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departament of Veterinary Pathology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Institute of Biodiversity and Forests Federal University of Western Pará (UFOPA)Aquaculture Center of UNESPDepartament of Veterinary Surgery and Clinic School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Departament of Veterinary Pathology School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)Aquaculture Center of UNESPDepartament of Veterinary Surgery and Clinic School of Agricultural and Veterinary Sciences São Paulo State University (UNESP)FAPESP: 2011/20280-2FAPESP: 2014/10231-2CNPq: 441054/2014-5Universidade Estadual Paulista (Unesp)Federal University of Western Pará (UFOPA)Marinho-Neto, Fausto A. [UNESP]Claudiano, Gustavo S. [UNESP]Yunis-Aguinaga, Jefferson [UNESP]Cueva-Quiroz, Victor A. [UNESP]Kobashigawa, Karina K. [UNESP]Cruz, Nathan R.N. [UNESP]Moraes, Flávio R. [UNESP]Moraes, Julieta R.E. [UNESP]2020-12-12T01:39:49Z2020-12-12T01:39:49Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0222626PLoS ONE, v. 14, n. 9, 2019.1932-6203http://hdl.handle.net/11449/19944210.1371/journal.pone.02226262-s2.0-85072520404Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2024-04-09T15:10:55Zoai:repositorio.unesp.br:11449/199442Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:10:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus |
title |
Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus |
spellingShingle |
Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus Marinho-Neto, Fausto A. [UNESP] |
title_short |
Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus |
title_full |
Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus |
title_fullStr |
Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus |
title_full_unstemmed |
Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus |
title_sort |
Morphological, microbiological and ultrastructural aspects of sepsis by Aeromonas hydrophila in Piaractus mesopotamicus |
author |
Marinho-Neto, Fausto A. [UNESP] |
author_facet |
Marinho-Neto, Fausto A. [UNESP] Claudiano, Gustavo S. [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Cueva-Quiroz, Victor A. [UNESP] Kobashigawa, Karina K. [UNESP] Cruz, Nathan R.N. [UNESP] Moraes, Flávio R. [UNESP] Moraes, Julieta R.E. [UNESP] |
author_role |
author |
author2 |
Claudiano, Gustavo S. [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Cueva-Quiroz, Victor A. [UNESP] Kobashigawa, Karina K. [UNESP] Cruz, Nathan R.N. [UNESP] Moraes, Flávio R. [UNESP] Moraes, Julieta R.E. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Western Pará (UFOPA) |
dc.contributor.author.fl_str_mv |
Marinho-Neto, Fausto A. [UNESP] Claudiano, Gustavo S. [UNESP] Yunis-Aguinaga, Jefferson [UNESP] Cueva-Quiroz, Victor A. [UNESP] Kobashigawa, Karina K. [UNESP] Cruz, Nathan R.N. [UNESP] Moraes, Flávio R. [UNESP] Moraes, Julieta R.E. [UNESP] |
description |
Aeromonas bacteria can cause an infection characterized by septicemia and is one of the most common pathogens in tropical fish. This disease is responsible for high morbidity and mortality rates, causing considerable losses in aquaculture. Thus, the understanding of its pathophysiology is crucial to develop control strategies of this bacterial infection in farmed fish. This study aimed to characterize early pathological aspects of acute sepsis in pacu (Piaractus mesopotamicus) experimentally infected with Aeromonas hydrophila. A total of 160 juvenile pacus were inoculated intraperitoneally with A. hydrophila (1.78 x 109 CFU/mL) and at 0 (control), 1, 3, 6, and 9 hours post-inoculation (hpi), animals were anesthetized and samples were collected for microbiological, light microscopy and transmission electron microscopy (TEM) analyzes. The results showed the occurrence of hemodynamic alterations, such as hemorrhage and congestion, which were observed mainly after 6 and 9 hpi. It was possible to re-isolate Aeromonas at all sampling times except in control group. However, just after 9 hpi it was possible to find the bacteria in all fish and tissues. Light microscopy analyses revealed a degenerative process, necrosis and vascular damage mainly at 6 and 9 hpi. According to the ultrastructural examination, areas of cellular death were identified in all examined tissues, especially at 6 and 9 hpi. However, the most severe, related to necrosis, were observed after 6 and 9 hpi. The findings suggested that this bacterium spreads in the first hpi through the fish organs, mainly affecting spleen, liver and kidney, causing irreversible lesions at the molecular level. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-01 2020-12-12T01:39:49Z 2020-12-12T01:39:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0222626 PLoS ONE, v. 14, n. 9, 2019. 1932-6203 http://hdl.handle.net/11449/199442 10.1371/journal.pone.0222626 2-s2.0-85072520404 |
url |
http://dx.doi.org/10.1371/journal.pone.0222626 http://hdl.handle.net/11449/199442 |
identifier_str_mv |
PLoS ONE, v. 14, n. 9, 2019. 1932-6203 10.1371/journal.pone.0222626 2-s2.0-85072520404 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964719437578240 |