Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3897/COMPCYTOGEN.V10I2.7939 http://hdl.handle.net/11449/228520 |
Resumo: | Characidium gomesi Travasso, 1956 specimens from the Pardo River have up to four heterochromatic supernumerary chromosomes, derived from the sex chromosomes. To access the meiotic behavior and distribution of an active chromatin marker, males and females of C. gomesi with two or three B chromosomes were analyzed. Mitotic chromosomes were characterized using C-banding and FISH with B chromosome probes. Meiocytes were subjected to immunofluorescence-FISH assay using anti-SYCP3, anti-H3K4m, and B chromosomes probes. Molecular homology of supernumeraries was confirmed by FISH and by its bivalent conformation in individuals with two of these chromosomes. In individuals with three Bs, these elements formed a bivalent and a univalent. Supernumerary and sex chromosomes exhibited H3K4m signals during pachytene contrasting with their heterochromatic and asynaptic nature, which suggest a more structural role than functional of this histone modification. The implications of this result are discussed in light of the homology, meiotic nuclear organization, and meiotic silencing of unsynapsed chomatin. |
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Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae)Chromosome paintingImmunodetectionSYCP3Synaptonemal complexCharacidium gomesi Travasso, 1956 specimens from the Pardo River have up to four heterochromatic supernumerary chromosomes, derived from the sex chromosomes. To access the meiotic behavior and distribution of an active chromatin marker, males and females of C. gomesi with two or three B chromosomes were analyzed. Mitotic chromosomes were characterized using C-banding and FISH with B chromosome probes. Meiocytes were subjected to immunofluorescence-FISH assay using anti-SYCP3, anti-H3K4m, and B chromosomes probes. Molecular homology of supernumeraries was confirmed by FISH and by its bivalent conformation in individuals with two of these chromosomes. In individuals with three Bs, these elements formed a bivalent and a univalent. Supernumerary and sex chromosomes exhibited H3K4m signals during pachytene contrasting with their heterochromatic and asynaptic nature, which suggest a more structural role than functional of this histone modification. The implications of this result are discussed in light of the homology, meiotic nuclear organization, and meiotic silencing of unsynapsed chomatin.Departamento de Morfologia Instituto de Biociências Universidade Estadual Paulista, Distrito de Rubião Junior, s/nInstituto de Ciencias Marinas y Limnólogicas Universidad Austral de Chile, Casilla 567Laboratório de Ecologia e Evolução Instituto Butantan, Avenida Vital Brazil, 1500, São PauloDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista, Distrito de Rubião Junior, s/nUniversidade Estadual Paulista (UNESP)Universidad Austral de ChileInstituto ButantanSerrano, érica Alves [UNESP]Araya-Jaime, Cristian [UNESP]Suárez-Villota, Elkin Y.Oliveira, Claudio [UNESP]Foresti, Fausto [UNESP]2022-04-29T08:27:14Z2022-04-29T08:27:14Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article255-268ahttp://dx.doi.org/10.3897/COMPCYTOGEN.V10I2.7939Comparative Cytogenetics, v. 10, n. 2, p. 255-268a, 2016.1993-078X1993-0771http://hdl.handle.net/11449/22852010.3897/COMPCYTOGEN.V10I2.79392-s2.0-85043624401Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogeneticsinfo:eu-repo/semantics/openAccess2022-04-29T08:27:15Zoai:repositorio.unesp.br:11449/228520Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:27:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae) |
title |
Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae) |
spellingShingle |
Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae) Serrano, érica Alves [UNESP] Chromosome painting Immunodetection SYCP3 Synaptonemal complex |
title_short |
Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae) |
title_full |
Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae) |
title_fullStr |
Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae) |
title_full_unstemmed |
Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae) |
title_sort |
Meiotic behavior and H3K4m distribution in B chromosomes of Characidium gomesi (Characiformes, Crenuchidae) |
author |
Serrano, érica Alves [UNESP] |
author_facet |
Serrano, érica Alves [UNESP] Araya-Jaime, Cristian [UNESP] Suárez-Villota, Elkin Y. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Araya-Jaime, Cristian [UNESP] Suárez-Villota, Elkin Y. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidad Austral de Chile Instituto Butantan |
dc.contributor.author.fl_str_mv |
Serrano, érica Alves [UNESP] Araya-Jaime, Cristian [UNESP] Suárez-Villota, Elkin Y. Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
Chromosome painting Immunodetection SYCP3 Synaptonemal complex |
topic |
Chromosome painting Immunodetection SYCP3 Synaptonemal complex |
description |
Characidium gomesi Travasso, 1956 specimens from the Pardo River have up to four heterochromatic supernumerary chromosomes, derived from the sex chromosomes. To access the meiotic behavior and distribution of an active chromatin marker, males and females of C. gomesi with two or three B chromosomes were analyzed. Mitotic chromosomes were characterized using C-banding and FISH with B chromosome probes. Meiocytes were subjected to immunofluorescence-FISH assay using anti-SYCP3, anti-H3K4m, and B chromosomes probes. Molecular homology of supernumeraries was confirmed by FISH and by its bivalent conformation in individuals with two of these chromosomes. In individuals with three Bs, these elements formed a bivalent and a univalent. Supernumerary and sex chromosomes exhibited H3K4m signals during pachytene contrasting with their heterochromatic and asynaptic nature, which suggest a more structural role than functional of this histone modification. The implications of this result are discussed in light of the homology, meiotic nuclear organization, and meiotic silencing of unsynapsed chomatin. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2022-04-29T08:27:14Z 2022-04-29T08:27:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3897/COMPCYTOGEN.V10I2.7939 Comparative Cytogenetics, v. 10, n. 2, p. 255-268a, 2016. 1993-078X 1993-0771 http://hdl.handle.net/11449/228520 10.3897/COMPCYTOGEN.V10I2.7939 2-s2.0-85043624401 |
url |
http://dx.doi.org/10.3897/COMPCYTOGEN.V10I2.7939 http://hdl.handle.net/11449/228520 |
identifier_str_mv |
Comparative Cytogenetics, v. 10, n. 2, p. 255-268a, 2016. 1993-078X 1993-0771 10.3897/COMPCYTOGEN.V10I2.7939 2-s2.0-85043624401 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Cytogenetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
255-268a |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964444117172224 |