Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-021-04121-1 http://hdl.handle.net/11449/233377 |
Resumo: | Objective: To assess the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the treatment of cancer. This study evaluated the influence of cisplatin (CIS) and 5-fluorouracil (5-FU) over the peri-implant tissues around osseointegrated titanium implants in animals previously exposed to nicotine. Materials and methods One hundred twenty male rats were divided into two groups, receiving via subcutaneous injection, either physiological saline solution (PSS) (n = 30) or nicotine hemissulfate (NIC) (n = 90) for 30 days prior to implants’ placement. One titanium implant (4.0 × 2.2 mm) was installed in each tibia of all animals. PSS and NIC were continued for 30 days after surgery. Five days after cessation, rats were subdivided into three subgroups in accordance with systemic treatments with either PSS, CIS, or 5-FU. Euthanasia was performed at 50, 65, and 95 days post-surgery. Histometric, histopathological, and immunohistochemical analyses were performed. Results: NIC-CIS and NIC-5FU presented lower BIC (50, 65, and 95 days) and bone area fraction occupancy (BAFO) (65 and 95 days) than group NIC. Intense inflammatory infiltration, severe tissue breakdown, reduced expression of bone formation biomarkers, and upregulation of TRAP were observed in NIC-CIS and NIC-5FU when compared with group NIC. TRAP expression was significantly higher in NIC-5FU as compared with NIC-CIS at 50 and 95 days. Groups NIC, NIC-CIS, and NIC-5FU presented statistically significant negative impact in all outcome parameters than group PSS. Conclusion: CIS and 5-FU severely disrupted the peri-implant tissues around osseointegrated implants in animals previously exposed to nicotine. Clinical relevance: Assessing the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the cancer treatment. |
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Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in ratsAntineoplastic agentsCisplatinDental implantsFluorouracilNicotineOsseointegrationObjective: To assess the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the treatment of cancer. This study evaluated the influence of cisplatin (CIS) and 5-fluorouracil (5-FU) over the peri-implant tissues around osseointegrated titanium implants in animals previously exposed to nicotine. Materials and methods One hundred twenty male rats were divided into two groups, receiving via subcutaneous injection, either physiological saline solution (PSS) (n = 30) or nicotine hemissulfate (NIC) (n = 90) for 30 days prior to implants’ placement. One titanium implant (4.0 × 2.2 mm) was installed in each tibia of all animals. PSS and NIC were continued for 30 days after surgery. Five days after cessation, rats were subdivided into three subgroups in accordance with systemic treatments with either PSS, CIS, or 5-FU. Euthanasia was performed at 50, 65, and 95 days post-surgery. Histometric, histopathological, and immunohistochemical analyses were performed. Results: NIC-CIS and NIC-5FU presented lower BIC (50, 65, and 95 days) and bone area fraction occupancy (BAFO) (65 and 95 days) than group NIC. Intense inflammatory infiltration, severe tissue breakdown, reduced expression of bone formation biomarkers, and upregulation of TRAP were observed in NIC-CIS and NIC-5FU when compared with group NIC. TRAP expression was significantly higher in NIC-5FU as compared with NIC-CIS at 50 and 95 days. Groups NIC, NIC-CIS, and NIC-5FU presented statistically significant negative impact in all outcome parameters than group PSS. Conclusion: CIS and 5-FU severely disrupted the peri-implant tissues around osseointegrated implants in animals previously exposed to nicotine. Clinical relevance: Assessing the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the cancer treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Diagnosis and Surgery–Periodontics Division School of Dentistry São Paulo State University (Unesp), St. José Bonifácio 1193, Vila Mendonça, SPNucleus of Study and Research in Periodontics and Implantology (NEPPI) School of Dentistry São Paulo State University (Unesp), SPDepartment of Basic Science School of Dentistry São Paulo State University (Unesp), SPDepartment of Oral and Maxillofacial Surgery Massachusetts General Hospital Harvard School of Dental MedicineDepartment of Diagnosis and Surgery–Periodontics Division School of Dentistry São Paulo State University (Unesp), St. José Bonifácio 1193, Vila Mendonça, SPNucleus of Study and Research in Periodontics and Implantology (NEPPI) School of Dentistry São Paulo State University (Unesp), SPDepartment of Basic Science School of Dentistry São Paulo State University (Unesp), SPFAPESP: 2014/11427-8FAPESP: 2017/11805-0Universidade Estadual Paulista (UNESP)Harvard School of Dental Medicinede Almeida, Juliano Milanezi [UNESP]Ervolino, Edilson [UNESP]Gusman, David Jonathan Rodrigues [UNESP]Fiorin, Luiz Guilherme [UNESP]Alves, Breno Edson Sendão [UNESP]Guastaldi, Fernando Pozzi SemeghiniMatheus, Henrique Rinaldi [UNESP]2022-05-01T08:15:11Z2022-05-01T08:15:11Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1477-1489http://dx.doi.org/10.1007/s00784-021-04121-1Clinical Oral Investigations, v. 26, n. 2, p. 1477-1489, 2022.1436-37711432-6981http://hdl.handle.net/11449/23337710.1007/s00784-021-04121-12-s2.0-85112348515Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2022-05-01T08:15:11Zoai:repositorio.unesp.br:11449/233377Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T08:15:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats |
title |
Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats |
spellingShingle |
Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats de Almeida, Juliano Milanezi [UNESP] Antineoplastic agents Cisplatin Dental implants Fluorouracil Nicotine Osseointegration |
title_short |
Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats |
title_full |
Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats |
title_fullStr |
Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats |
title_full_unstemmed |
Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats |
title_sort |
Antineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rats |
author |
de Almeida, Juliano Milanezi [UNESP] |
author_facet |
de Almeida, Juliano Milanezi [UNESP] Ervolino, Edilson [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Fiorin, Luiz Guilherme [UNESP] Alves, Breno Edson Sendão [UNESP] Guastaldi, Fernando Pozzi Semeghini Matheus, Henrique Rinaldi [UNESP] |
author_role |
author |
author2 |
Ervolino, Edilson [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Fiorin, Luiz Guilherme [UNESP] Alves, Breno Edson Sendão [UNESP] Guastaldi, Fernando Pozzi Semeghini Matheus, Henrique Rinaldi [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Harvard School of Dental Medicine |
dc.contributor.author.fl_str_mv |
de Almeida, Juliano Milanezi [UNESP] Ervolino, Edilson [UNESP] Gusman, David Jonathan Rodrigues [UNESP] Fiorin, Luiz Guilherme [UNESP] Alves, Breno Edson Sendão [UNESP] Guastaldi, Fernando Pozzi Semeghini Matheus, Henrique Rinaldi [UNESP] |
dc.subject.por.fl_str_mv |
Antineoplastic agents Cisplatin Dental implants Fluorouracil Nicotine Osseointegration |
topic |
Antineoplastic agents Cisplatin Dental implants Fluorouracil Nicotine Osseointegration |
description |
Objective: To assess the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the treatment of cancer. This study evaluated the influence of cisplatin (CIS) and 5-fluorouracil (5-FU) over the peri-implant tissues around osseointegrated titanium implants in animals previously exposed to nicotine. Materials and methods One hundred twenty male rats were divided into two groups, receiving via subcutaneous injection, either physiological saline solution (PSS) (n = 30) or nicotine hemissulfate (NIC) (n = 90) for 30 days prior to implants’ placement. One titanium implant (4.0 × 2.2 mm) was installed in each tibia of all animals. PSS and NIC were continued for 30 days after surgery. Five days after cessation, rats were subdivided into three subgroups in accordance with systemic treatments with either PSS, CIS, or 5-FU. Euthanasia was performed at 50, 65, and 95 days post-surgery. Histometric, histopathological, and immunohistochemical analyses were performed. Results: NIC-CIS and NIC-5FU presented lower BIC (50, 65, and 95 days) and bone area fraction occupancy (BAFO) (65 and 95 days) than group NIC. Intense inflammatory infiltration, severe tissue breakdown, reduced expression of bone formation biomarkers, and upregulation of TRAP were observed in NIC-CIS and NIC-5FU when compared with group NIC. TRAP expression was significantly higher in NIC-5FU as compared with NIC-CIS at 50 and 95 days. Groups NIC, NIC-CIS, and NIC-5FU presented statistically significant negative impact in all outcome parameters than group PSS. Conclusion: CIS and 5-FU severely disrupted the peri-implant tissues around osseointegrated implants in animals previously exposed to nicotine. Clinical relevance: Assessing the interaction between chemotherapy and normal tissues is critical to assure quality of life during and after the cancer treatment. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T08:15:11Z 2022-05-01T08:15:11Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-021-04121-1 Clinical Oral Investigations, v. 26, n. 2, p. 1477-1489, 2022. 1436-3771 1432-6981 http://hdl.handle.net/11449/233377 10.1007/s00784-021-04121-1 2-s2.0-85112348515 |
url |
http://dx.doi.org/10.1007/s00784-021-04121-1 http://hdl.handle.net/11449/233377 |
identifier_str_mv |
Clinical Oral Investigations, v. 26, n. 2, p. 1477-1489, 2022. 1436-3771 1432-6981 10.1007/s00784-021-04121-1 2-s2.0-85112348515 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1477-1489 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1792961553299931136 |