Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis
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Publication Date: | 2008 |
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Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/439 http://hdl.handle.net/11449/70618 |
Summary: | P. brasiliensis parasitizes various human tissues and proteinases exported by this fungus may allow it to metabolize and invade host tissues. The influence of the culture medium on the production of proteinases by P. brasiliensis isolates was studied and the export of these enzymes was followed as a function of culture time. The fungus was grown in neopeptone, BSA, elastin or collagen medium. The culture medium was assayed for azocollytic, elastinolytic and caseinolytic activity. Proteolytic activity was also analysed by electrophoresis of the culture medium on gelatin and casein substrate gels. P. brasiliensis expressed relatively high levels of azocoll, elastin and casein degrading activity in all types of medium, except in neopeptone medium. Generally, expression of azocollytic activity peaked during the third week of culture and caseinolytic activity during the fourth week of culture. Azocollytic activity was highest at pH 4.0 and caseinolytic activity at pH 8.0. Elastinolytic activity was also highest at pH 8.0. This activity, as well as the others, may provide the fungus with a source of carbon and nitrogen and may also be responsible for the invasion of host tissues, such as pulmonary elastic fiber, by P. brasiliensis. |
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Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensisCollagenolytic activityElastinolytic activityParacoccidioides brasiliensisParacoccidioidomycosisProteolytic activitycarboncaseinelastingelatinnitrogenculture mediumelastic fiberelastolysisfungus culturefungus growthfungus isolationnonhumanpHpolyacrylamide gel electrophoresisprotein degradationprotein inductionprotein secretionprotein synthesisP. brasiliensis parasitizes various human tissues and proteinases exported by this fungus may allow it to metabolize and invade host tissues. The influence of the culture medium on the production of proteinases by P. brasiliensis isolates was studied and the export of these enzymes was followed as a function of culture time. The fungus was grown in neopeptone, BSA, elastin or collagen medium. The culture medium was assayed for azocollytic, elastinolytic and caseinolytic activity. Proteolytic activity was also analysed by electrophoresis of the culture medium on gelatin and casein substrate gels. P. brasiliensis expressed relatively high levels of azocoll, elastin and casein degrading activity in all types of medium, except in neopeptone medium. Generally, expression of azocollytic activity peaked during the third week of culture and caseinolytic activity during the fourth week of culture. Azocollytic activity was highest at pH 4.0 and caseinolytic activity at pH 8.0. Elastinolytic activity was also highest at pH 8.0. This activity, as well as the others, may provide the fungus with a source of carbon and nitrogen and may also be responsible for the invasion of host tissues, such as pulmonary elastic fiber, by P. brasiliensis.Laboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista, UNESP, Araraquara, SPLaboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista, UNESP, Rua Expedicionários do Brasil, 1621, CEP: 14801-902 - Araraquara - SPLaboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista, UNESP, Araraquara, SPLaboratório de Micologia Clínica Departamento de Análises Clínicas Universidade Estadual Paulista, UNESP, Rua Expedicionários do Brasil, 1621, CEP: 14801-902 - Araraquara - SPUniversidade Estadual Paulista (Unesp)Voltan, A. R. [UNESP]Donofrio, F. [UNESP]Miranda, E. T. [UNESP]Moraes, R. A. [UNESP]Mendes-Giannini, Maria José Soares [UNESP]2014-05-27T11:23:41Z2014-05-27T11:23:41Z2008-10-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article97-106application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/439Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 29, n. 1, p. 97-106, 2008.1808-45322179-443Xhttp://hdl.handle.net/11449/706182-s2.0-543491192422-s2.0-54349119242.pdf0000-0002-8059-0826Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2023-12-15T06:21:21Zoai:repositorio.unesp.br:11449/70618Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-15T06:21:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis |
title |
Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis |
spellingShingle |
Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis Voltan, A. R. [UNESP] Collagenolytic activity Elastinolytic activity Paracoccidioides brasiliensis Paracoccidioidomycosis Proteolytic activity carbon casein elastin gelatin nitrogen culture medium elastic fiber elastolysis fungus culture fungus growth fungus isolation nonhuman pH polyacrylamide gel electrophoresis protein degradation protein induction protein secretion protein synthesis |
title_short |
Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis |
title_full |
Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis |
title_fullStr |
Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis |
title_full_unstemmed |
Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis |
title_sort |
Induction and secretion of elastinolytic and proteolytic activity in cultures of Paracoccidioides brasiliensis |
author |
Voltan, A. R. [UNESP] |
author_facet |
Voltan, A. R. [UNESP] Donofrio, F. [UNESP] Miranda, E. T. [UNESP] Moraes, R. A. [UNESP] Mendes-Giannini, Maria José Soares [UNESP] |
author_role |
author |
author2 |
Donofrio, F. [UNESP] Miranda, E. T. [UNESP] Moraes, R. A. [UNESP] Mendes-Giannini, Maria José Soares [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Voltan, A. R. [UNESP] Donofrio, F. [UNESP] Miranda, E. T. [UNESP] Moraes, R. A. [UNESP] Mendes-Giannini, Maria José Soares [UNESP] |
dc.subject.por.fl_str_mv |
Collagenolytic activity Elastinolytic activity Paracoccidioides brasiliensis Paracoccidioidomycosis Proteolytic activity carbon casein elastin gelatin nitrogen culture medium elastic fiber elastolysis fungus culture fungus growth fungus isolation nonhuman pH polyacrylamide gel electrophoresis protein degradation protein induction protein secretion protein synthesis |
topic |
Collagenolytic activity Elastinolytic activity Paracoccidioides brasiliensis Paracoccidioidomycosis Proteolytic activity carbon casein elastin gelatin nitrogen culture medium elastic fiber elastolysis fungus culture fungus growth fungus isolation nonhuman pH polyacrylamide gel electrophoresis protein degradation protein induction protein secretion protein synthesis |
description |
P. brasiliensis parasitizes various human tissues and proteinases exported by this fungus may allow it to metabolize and invade host tissues. The influence of the culture medium on the production of proteinases by P. brasiliensis isolates was studied and the export of these enzymes was followed as a function of culture time. The fungus was grown in neopeptone, BSA, elastin or collagen medium. The culture medium was assayed for azocollytic, elastinolytic and caseinolytic activity. Proteolytic activity was also analysed by electrophoresis of the culture medium on gelatin and casein substrate gels. P. brasiliensis expressed relatively high levels of azocoll, elastin and casein degrading activity in all types of medium, except in neopeptone medium. Generally, expression of azocollytic activity peaked during the third week of culture and caseinolytic activity during the fourth week of culture. Azocollytic activity was highest at pH 4.0 and caseinolytic activity at pH 8.0. Elastinolytic activity was also highest at pH 8.0. This activity, as well as the others, may provide the fungus with a source of carbon and nitrogen and may also be responsible for the invasion of host tissues, such as pulmonary elastic fiber, by P. brasiliensis. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-28 2014-05-27T11:23:41Z 2014-05-27T11:23:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/439 Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 29, n. 1, p. 97-106, 2008. 1808-4532 2179-443X http://hdl.handle.net/11449/70618 2-s2.0-54349119242 2-s2.0-54349119242.pdf 0000-0002-8059-0826 |
url |
http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/439 http://hdl.handle.net/11449/70618 |
identifier_str_mv |
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 29, n. 1, p. 97-106, 2008. 1808-4532 2179-443X 2-s2.0-54349119242 2-s2.0-54349119242.pdf 0000-0002-8059-0826 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista de Ciências Farmacêuticas Básica e Aplicada 0,131 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
97-106 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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