Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://scidoc.org/IJDOS-2377-8075-S1-001.php http://hdl.handle.net/11449/126078 |
Resumo: | The purpose of this study was to evaluate the effectiveness of complementary heat treatment and water storage in reducing cytotoxicity of acrylic resins denture bases used in Brazil by the MTT assay. Material and Methods: First, nine specimens were fabricated from metal matrix in the form of discs with 14 mm in diameter and 1.2 mm of thick. Immediately after making, 24 or 48 hours after storage in distilled water, the samples of heat-polymerized resins were divided into 3 groups (n = 3) according to the type of thermal treatment: Group 1: samples were individually exposed to microwave energy (500 W for 3 minutes); Group 2: samples were immersed in water at 550 C for 60 minutes; Group 3: samples did not receive heat treatment. To prepare the extracts, 3 samples of each group were placed into vials containing 3 mL of culture medium and stored at 37°C for 24 hours. L929 cells were used and the MTT assay was performed to analyze the cellular metabolism. Two-factor analysis of variance was used to detect significant among groups at 5% significance. Results: After statistical analysis, the materials were classified according to the cytotoxic effect: non-cytotoxic, slightly cytotoxic; moderately cytotoxic; and strongly cytotoxic. The results showed that the resins ranged from moderately cytotoxic to non-cytotoxic, but no statistically significant difference among experimental groups. Furthermore, the water storage and thermal treatments reduced the cytotoxicity of the resins. Conclusions: It was concluded that the resins studied are potentially toxic and that treatments can decrease their cytotoxicity. |
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Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resinsAcrylic resinHeat treatmentCytotoxicityThe purpose of this study was to evaluate the effectiveness of complementary heat treatment and water storage in reducing cytotoxicity of acrylic resins denture bases used in Brazil by the MTT assay. Material and Methods: First, nine specimens were fabricated from metal matrix in the form of discs with 14 mm in diameter and 1.2 mm of thick. Immediately after making, 24 or 48 hours after storage in distilled water, the samples of heat-polymerized resins were divided into 3 groups (n = 3) according to the type of thermal treatment: Group 1: samples were individually exposed to microwave energy (500 W for 3 minutes); Group 2: samples were immersed in water at 550 C for 60 minutes; Group 3: samples did not receive heat treatment. To prepare the extracts, 3 samples of each group were placed into vials containing 3 mL of culture medium and stored at 37°C for 24 hours. L929 cells were used and the MTT assay was performed to analyze the cellular metabolism. Two-factor analysis of variance was used to detect significant among groups at 5% significance. Results: After statistical analysis, the materials were classified according to the cytotoxic effect: non-cytotoxic, slightly cytotoxic; moderately cytotoxic; and strongly cytotoxic. The results showed that the resins ranged from moderately cytotoxic to non-cytotoxic, but no statistically significant difference among experimental groups. Furthermore, the water storage and thermal treatments reduced the cytotoxicity of the resins. Conclusions: It was concluded that the resins studied are potentially toxic and that treatments can decrease their cytotoxicity.Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801903, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801903, SP, BrasilMateriais Odontológicos e PróteseUniversidade Estadual Paulista (Unesp)Silva, Carla Regina Couto [UNESP]Pellissari, Cláudia Viviane Guimarães [UNESP]Sanitá, Paula Volpato [UNESP]Arbeláez, María Isabel Amaya [UNESP]Massetti, Paula [UNESP]Jorge, Janaina Habib [UNESP]2015-08-06T16:13:53Z2015-08-06T16:13:53Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8application/pdfhttp://scidoc.org/IJDOS-2377-8075-S1-001.phpInternational Journal of Dentistry and Oral Science, v. 2, n. 4, p. 1-8, 2015.2377-8075http://hdl.handle.net/11449/126078ISSN2377-8075-2015-02-04-01-08.pdf8605087609369467Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Dentistry and Oral Scienceinfo:eu-repo/semantics/openAccess2023-12-29T06:22:51Zoai:repositorio.unesp.br:11449/126078Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-29T06:22:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins |
title |
Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins |
spellingShingle |
Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins Silva, Carla Regina Couto [UNESP] Acrylic resin Heat treatment Cytotoxicity |
title_short |
Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins |
title_full |
Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins |
title_fullStr |
Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins |
title_full_unstemmed |
Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins |
title_sort |
Metabolism of L929 cells after contact with acrylic resins. Part 1: Acrylic denture base resins |
author |
Silva, Carla Regina Couto [UNESP] |
author_facet |
Silva, Carla Regina Couto [UNESP] Pellissari, Cláudia Viviane Guimarães [UNESP] Sanitá, Paula Volpato [UNESP] Arbeláez, María Isabel Amaya [UNESP] Massetti, Paula [UNESP] Jorge, Janaina Habib [UNESP] |
author_role |
author |
author2 |
Pellissari, Cláudia Viviane Guimarães [UNESP] Sanitá, Paula Volpato [UNESP] Arbeláez, María Isabel Amaya [UNESP] Massetti, Paula [UNESP] Jorge, Janaina Habib [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Carla Regina Couto [UNESP] Pellissari, Cláudia Viviane Guimarães [UNESP] Sanitá, Paula Volpato [UNESP] Arbeláez, María Isabel Amaya [UNESP] Massetti, Paula [UNESP] Jorge, Janaina Habib [UNESP] |
dc.subject.por.fl_str_mv |
Acrylic resin Heat treatment Cytotoxicity |
topic |
Acrylic resin Heat treatment Cytotoxicity |
description |
The purpose of this study was to evaluate the effectiveness of complementary heat treatment and water storage in reducing cytotoxicity of acrylic resins denture bases used in Brazil by the MTT assay. Material and Methods: First, nine specimens were fabricated from metal matrix in the form of discs with 14 mm in diameter and 1.2 mm of thick. Immediately after making, 24 or 48 hours after storage in distilled water, the samples of heat-polymerized resins were divided into 3 groups (n = 3) according to the type of thermal treatment: Group 1: samples were individually exposed to microwave energy (500 W for 3 minutes); Group 2: samples were immersed in water at 550 C for 60 minutes; Group 3: samples did not receive heat treatment. To prepare the extracts, 3 samples of each group were placed into vials containing 3 mL of culture medium and stored at 37°C for 24 hours. L929 cells were used and the MTT assay was performed to analyze the cellular metabolism. Two-factor analysis of variance was used to detect significant among groups at 5% significance. Results: After statistical analysis, the materials were classified according to the cytotoxic effect: non-cytotoxic, slightly cytotoxic; moderately cytotoxic; and strongly cytotoxic. The results showed that the resins ranged from moderately cytotoxic to non-cytotoxic, but no statistically significant difference among experimental groups. Furthermore, the water storage and thermal treatments reduced the cytotoxicity of the resins. Conclusions: It was concluded that the resins studied are potentially toxic and that treatments can decrease their cytotoxicity. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-06T16:13:53Z 2015-08-06T16:13:53Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scidoc.org/IJDOS-2377-8075-S1-001.php International Journal of Dentistry and Oral Science, v. 2, n. 4, p. 1-8, 2015. 2377-8075 http://hdl.handle.net/11449/126078 ISSN2377-8075-2015-02-04-01-08.pdf 8605087609369467 |
url |
http://scidoc.org/IJDOS-2377-8075-S1-001.php http://hdl.handle.net/11449/126078 |
identifier_str_mv |
International Journal of Dentistry and Oral Science, v. 2, n. 4, p. 1-8, 2015. 2377-8075 ISSN2377-8075-2015-02-04-01-08.pdf 8605087609369467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Dentistry and Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792962206930829312 |