Effect of hydrogen peroxide on thawed ovine sperm motility
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v11n2/pag119-123%20(AR654).pdf http://hdl.handle.net/11449/117701 |
Resumo: | Oxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen peroxide (H2O2) in ovine sperm motility after thawing and catalase (CAT) ability to preserve sperm motility. Semen was incubated at 37 degrees C with 100 mu M H2O2, 1 U catalase + 100 mu M H2O2 or no treatment, for 30 min. Immediately after adding treatments, sperm motility was determined by computer-assisted semen analysis (CASA). Incubation with H2O2 led to a significant (P < 0.05) decrease in motility parameters whereas catalase prevented a decline in motility secondary to oxidative stress. After 30 min of incubation with H2O2, total motility (12.0% vs. control 73.0%, H2O2 +CAT 70.0%), progressive motility (0.0% vs. control 19.0%, H2O2 +CAT 19.0%) and rapid motility (1.0% vs. control 43.0%, H2O2 +CAT 40.0%) decreased significantly (P < 0.05), whereas percentage of static cells increased (84.0% vs. control 18.0%, H2O2 +CAT 20.0%). We conclude that H2O2 causes damage to ovine sperm motility and that catalase is able to avoid detrimental effect of H2O2 on sperm motility. |
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Repositório Institucional da UNESP |
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Effect of hydrogen peroxide on thawed ovine sperm motilityantioxidantscatalaseoxidative stressROSOxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen peroxide (H2O2) in ovine sperm motility after thawing and catalase (CAT) ability to preserve sperm motility. Semen was incubated at 37 degrees C with 100 mu M H2O2, 1 U catalase + 100 mu M H2O2 or no treatment, for 30 min. Immediately after adding treatments, sperm motility was determined by computer-assisted semen analysis (CASA). Incubation with H2O2 led to a significant (P < 0.05) decrease in motility parameters whereas catalase prevented a decline in motility secondary to oxidative stress. After 30 min of incubation with H2O2, total motility (12.0% vs. control 73.0%, H2O2 +CAT 70.0%), progressive motility (0.0% vs. control 19.0%, H2O2 +CAT 19.0%) and rapid motility (1.0% vs. control 43.0%, H2O2 +CAT 40.0%) decreased significantly (P < 0.05), whereas percentage of static cells increased (84.0% vs. control 18.0%, H2O2 +CAT 20.0%). We conclude that H2O2 causes damage to ovine sperm motility and that catalase is able to avoid detrimental effect of H2O2 on sperm motility.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Agr Res Corp EMBRAPA, Semiarid Res Ctr, Petrolina, PE, BrazilSao Paulo State Univ, Fac Vet Med & Anim Sci, Botucatu, SP, BrazilSao Paulo State Univ, Fac Vet Med & Anim Sci, Botucatu, SP, BrazilBrazilian Coll Animal ReproductionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Maia, Marciane da SilvaBicudo, Sony Dimas [UNESP]Rodello, Leandro [UNESP]2015-03-18T15:56:48Z2015-03-18T15:56:48Z2014-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article119-123application/pdfhttp://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v11n2/pag119-123%20(AR654).pdfAnimal Reproduction. Belo Horizonte: Brazilian Coll Animal Reproduction, v. 11, n. 2, p. 119-123, 2014.1806-9614http://hdl.handle.net/11449/117701WOS:000346368100007WOS000346368100007.pdf8134606270913860Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction0.9910,308info:eu-repo/semantics/openAccess2024-01-21T06:22:43Zoai:repositorio.unesp.br:11449/117701Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-21T06:22:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of hydrogen peroxide on thawed ovine sperm motility |
title |
Effect of hydrogen peroxide on thawed ovine sperm motility |
spellingShingle |
Effect of hydrogen peroxide on thawed ovine sperm motility Maia, Marciane da Silva antioxidants catalase oxidative stress ROS |
title_short |
Effect of hydrogen peroxide on thawed ovine sperm motility |
title_full |
Effect of hydrogen peroxide on thawed ovine sperm motility |
title_fullStr |
Effect of hydrogen peroxide on thawed ovine sperm motility |
title_full_unstemmed |
Effect of hydrogen peroxide on thawed ovine sperm motility |
title_sort |
Effect of hydrogen peroxide on thawed ovine sperm motility |
author |
Maia, Marciane da Silva |
author_facet |
Maia, Marciane da Silva Bicudo, Sony Dimas [UNESP] Rodello, Leandro [UNESP] |
author_role |
author |
author2 |
Bicudo, Sony Dimas [UNESP] Rodello, Leandro [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Maia, Marciane da Silva Bicudo, Sony Dimas [UNESP] Rodello, Leandro [UNESP] |
dc.subject.por.fl_str_mv |
antioxidants catalase oxidative stress ROS |
topic |
antioxidants catalase oxidative stress ROS |
description |
Oxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen peroxide (H2O2) in ovine sperm motility after thawing and catalase (CAT) ability to preserve sperm motility. Semen was incubated at 37 degrees C with 100 mu M H2O2, 1 U catalase + 100 mu M H2O2 or no treatment, for 30 min. Immediately after adding treatments, sperm motility was determined by computer-assisted semen analysis (CASA). Incubation with H2O2 led to a significant (P < 0.05) decrease in motility parameters whereas catalase prevented a decline in motility secondary to oxidative stress. After 30 min of incubation with H2O2, total motility (12.0% vs. control 73.0%, H2O2 +CAT 70.0%), progressive motility (0.0% vs. control 19.0%, H2O2 +CAT 19.0%) and rapid motility (1.0% vs. control 43.0%, H2O2 +CAT 40.0%) decreased significantly (P < 0.05), whereas percentage of static cells increased (84.0% vs. control 18.0%, H2O2 +CAT 20.0%). We conclude that H2O2 causes damage to ovine sperm motility and that catalase is able to avoid detrimental effect of H2O2 on sperm motility. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 2015-03-18T15:56:48Z 2015-03-18T15:56:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v11n2/pag119-123%20(AR654).pdf Animal Reproduction. Belo Horizonte: Brazilian Coll Animal Reproduction, v. 11, n. 2, p. 119-123, 2014. 1806-9614 http://hdl.handle.net/11449/117701 WOS:000346368100007 WOS000346368100007.pdf 8134606270913860 |
url |
http://www.cbra.org.br/pages/publicacoes/animalreproduction/issues/download/v11n2/pag119-123%20(AR654).pdf http://hdl.handle.net/11449/117701 |
identifier_str_mv |
Animal Reproduction. Belo Horizonte: Brazilian Coll Animal Reproduction, v. 11, n. 2, p. 119-123, 2014. 1806-9614 WOS:000346368100007 WOS000346368100007.pdf 8134606270913860 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction 0.991 0,308 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
119-123 application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Coll Animal Reproduction |
publisher.none.fl_str_mv |
Brazilian Coll Animal Reproduction |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792962395049558016 |