Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation

Bibliographic Details
Main Author: Spirandeli, B. R.
Publication Date: 2021
Other Authors: Ribas, R. G., Amaral, S. S. [UNESP], Martins, E. F., Esposito, E., Vasconcellos, L. M.R. [UNESP], Campos, T. M.B., Thim, G. P., Trichês, E. S.
Format: Article
Language: eng
Source: Repositório Institucional da UNESP
Download full: http://dx.doi.org/10.1016/j.msec.2021.112453
http://hdl.handle.net/11449/222694
Summary: In this work, β-TCP (β-tricalcium phosphate) bioresorbable scaffolds were prepared by the gel casting method. Then, they were impregnated with a 45S5 bioglass sol gel solution to improve biocompatibility and promote bioactivity and antimicrobial activity. The β-TCP scaffolds had an apparent porosity of 72%, and after the incorporation of the bioglass, this porosity was maintained. The elements of the bioglass were incorporated into β-TCP matrix and there was a partial transformation from the β-TCP phase to the α-TCP (α-tricalcium phosphate) phase, besides the formation of bioactive calcium and sodium‑calcium silicates. The scaffolds β-TCP with 45S5 bioglass incorporated (β-TCP/45S5) did not show a reduction in their values of mechanical strength and Weibull modulus, despite the partial transformation to the α-TCP phase. Bioactivity, cell viability, and antimicrobial activity improved significantly for the β-TCP/45S5 scaffold comparing to the scaffold without the bioglass. The mineralization of carbonated hydroxyapatite was verified in Simulated Body Fluid (SBF). The cell viability, evaluated by the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide – MTT in MG63 cells, increased by 178%, and β-TCP/45S5 scaffold also enhanced cell activity and osteoblast differentiation observed by means of total protein contend and alkaline phosphatase activity, respectively. The formation of growth inhibition zones was also observed in the disk diffusion assay for three tested microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. To conclude, the vacuum impregnation method in 45S5 bioglass sol gel solution was effective in penetrating all the interconnected macroporosity of the scaffolds and covering the surface of the struts, which improved their biological properties in vitro, bioactivity and antibacterial activity, without reducing mechanical strength and porosity values. Thus, the β-TCP/45S5 scaffolds are shown as potential candidates for use in tissue engineering, mainly in bone tissue regeneration and recovery.
id UNSP_887f068f41658bd0a10c69316d358a3a
oai_identifier_str oai:repositorio.unesp.br:11449/222694
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation45S5 bioglassAntimicrobial activityBioactivityBiocompatibilityScaffoldsSol-gelTricalcium phosphateIn this work, β-TCP (β-tricalcium phosphate) bioresorbable scaffolds were prepared by the gel casting method. Then, they were impregnated with a 45S5 bioglass sol gel solution to improve biocompatibility and promote bioactivity and antimicrobial activity. The β-TCP scaffolds had an apparent porosity of 72%, and after the incorporation of the bioglass, this porosity was maintained. The elements of the bioglass were incorporated into β-TCP matrix and there was a partial transformation from the β-TCP phase to the α-TCP (α-tricalcium phosphate) phase, besides the formation of bioactive calcium and sodium‑calcium silicates. The scaffolds β-TCP with 45S5 bioglass incorporated (β-TCP/45S5) did not show a reduction in their values of mechanical strength and Weibull modulus, despite the partial transformation to the α-TCP phase. Bioactivity, cell viability, and antimicrobial activity improved significantly for the β-TCP/45S5 scaffold comparing to the scaffold without the bioglass. The mineralization of carbonated hydroxyapatite was verified in Simulated Body Fluid (SBF). The cell viability, evaluated by the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide – MTT in MG63 cells, increased by 178%, and β-TCP/45S5 scaffold also enhanced cell activity and osteoblast differentiation observed by means of total protein contend and alkaline phosphatase activity, respectively. The formation of growth inhibition zones was also observed in the disk diffusion assay for three tested microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. To conclude, the vacuum impregnation method in 45S5 bioglass sol gel solution was effective in penetrating all the interconnected macroporosity of the scaffolds and covering the surface of the struts, which improved their biological properties in vitro, bioactivity and antibacterial activity, without reducing mechanical strength and porosity values. Thus, the β-TCP/45S5 scaffolds are shown as potential candidates for use in tissue engineering, mainly in bone tissue regeneration and recovery.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Federal University of São Paulo (UNIFESP), 330 Talim StTechnological Institute of Aeronautics (ITA), 50 Mal. Eduardo Gomes PlazaSão Paulo State University (UNESP) Institute of Science and Technology, 777 Eng. Francisco José Longo AvenueSão Paulo State University (UNESP) Institute of Science and Technology, 777 Eng. Francisco José Longo AvenueFAPESP: 2015/24659-7Universidade de São Paulo (USP)Technological Institute of Aeronautics (ITA)Universidade Estadual Paulista (UNESP)Spirandeli, B. R.Ribas, R. G.Amaral, S. S. [UNESP]Martins, E. F.Esposito, E.Vasconcellos, L. M.R. [UNESP]Campos, T. M.B.Thim, G. P.Trichês, E. S.2022-04-28T19:46:08Z2022-04-28T19:46:08Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.msec.2021.112453Materials Science and Engineering C, v. 131.1873-01910928-4931http://hdl.handle.net/11449/22269410.1016/j.msec.2021.1124532-s2.0-85117565189Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2022-04-28T19:46:08Zoai:repositorio.unesp.br:11449/222694Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:17:52.439485Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
title Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
spellingShingle Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
Spirandeli, B. R.
45S5 bioglass
Antimicrobial activity
Bioactivity
Biocompatibility
Scaffolds
Sol-gel
Tricalcium phosphate
title_short Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
title_full Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
title_fullStr Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
title_full_unstemmed Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
title_sort Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
author Spirandeli, B. R.
author_facet Spirandeli, B. R.
Ribas, R. G.
Amaral, S. S. [UNESP]
Martins, E. F.
Esposito, E.
Vasconcellos, L. M.R. [UNESP]
Campos, T. M.B.
Thim, G. P.
Trichês, E. S.
author_role author
author2 Ribas, R. G.
Amaral, S. S. [UNESP]
Martins, E. F.
Esposito, E.
Vasconcellos, L. M.R. [UNESP]
Campos, T. M.B.
Thim, G. P.
Trichês, E. S.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Technological Institute of Aeronautics (ITA)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Spirandeli, B. R.
Ribas, R. G.
Amaral, S. S. [UNESP]
Martins, E. F.
Esposito, E.
Vasconcellos, L. M.R. [UNESP]
Campos, T. M.B.
Thim, G. P.
Trichês, E. S.
dc.subject.por.fl_str_mv 45S5 bioglass
Antimicrobial activity
Bioactivity
Biocompatibility
Scaffolds
Sol-gel
Tricalcium phosphate
topic 45S5 bioglass
Antimicrobial activity
Bioactivity
Biocompatibility
Scaffolds
Sol-gel
Tricalcium phosphate
description In this work, β-TCP (β-tricalcium phosphate) bioresorbable scaffolds were prepared by the gel casting method. Then, they were impregnated with a 45S5 bioglass sol gel solution to improve biocompatibility and promote bioactivity and antimicrobial activity. The β-TCP scaffolds had an apparent porosity of 72%, and after the incorporation of the bioglass, this porosity was maintained. The elements of the bioglass were incorporated into β-TCP matrix and there was a partial transformation from the β-TCP phase to the α-TCP (α-tricalcium phosphate) phase, besides the formation of bioactive calcium and sodium‑calcium silicates. The scaffolds β-TCP with 45S5 bioglass incorporated (β-TCP/45S5) did not show a reduction in their values of mechanical strength and Weibull modulus, despite the partial transformation to the α-TCP phase. Bioactivity, cell viability, and antimicrobial activity improved significantly for the β-TCP/45S5 scaffold comparing to the scaffold without the bioglass. The mineralization of carbonated hydroxyapatite was verified in Simulated Body Fluid (SBF). The cell viability, evaluated by the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide – MTT in MG63 cells, increased by 178%, and β-TCP/45S5 scaffold also enhanced cell activity and osteoblast differentiation observed by means of total protein contend and alkaline phosphatase activity, respectively. The formation of growth inhibition zones was also observed in the disk diffusion assay for three tested microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. To conclude, the vacuum impregnation method in 45S5 bioglass sol gel solution was effective in penetrating all the interconnected macroporosity of the scaffolds and covering the surface of the struts, which improved their biological properties in vitro, bioactivity and antibacterial activity, without reducing mechanical strength and porosity values. Thus, the β-TCP/45S5 scaffolds are shown as potential candidates for use in tissue engineering, mainly in bone tissue regeneration and recovery.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-01
2022-04-28T19:46:08Z
2022-04-28T19:46:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.msec.2021.112453
Materials Science and Engineering C, v. 131.
1873-0191
0928-4931
http://hdl.handle.net/11449/222694
10.1016/j.msec.2021.112453
2-s2.0-85117565189
url http://dx.doi.org/10.1016/j.msec.2021.112453
http://hdl.handle.net/11449/222694
identifier_str_mv Materials Science and Engineering C, v. 131.
1873-0191
0928-4931
10.1016/j.msec.2021.112453
2-s2.0-85117565189
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials Science and Engineering C
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129048383062016