Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation
Main Author: | |
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Publication Date: | 2021 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.msec.2021.112453 http://hdl.handle.net/11449/222694 |
Summary: | In this work, β-TCP (β-tricalcium phosphate) bioresorbable scaffolds were prepared by the gel casting method. Then, they were impregnated with a 45S5 bioglass sol gel solution to improve biocompatibility and promote bioactivity and antimicrobial activity. The β-TCP scaffolds had an apparent porosity of 72%, and after the incorporation of the bioglass, this porosity was maintained. The elements of the bioglass were incorporated into β-TCP matrix and there was a partial transformation from the β-TCP phase to the α-TCP (α-tricalcium phosphate) phase, besides the formation of bioactive calcium and sodium‑calcium silicates. The scaffolds β-TCP with 45S5 bioglass incorporated (β-TCP/45S5) did not show a reduction in their values of mechanical strength and Weibull modulus, despite the partial transformation to the α-TCP phase. Bioactivity, cell viability, and antimicrobial activity improved significantly for the β-TCP/45S5 scaffold comparing to the scaffold without the bioglass. The mineralization of carbonated hydroxyapatite was verified in Simulated Body Fluid (SBF). The cell viability, evaluated by the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide – MTT in MG63 cells, increased by 178%, and β-TCP/45S5 scaffold also enhanced cell activity and osteoblast differentiation observed by means of total protein contend and alkaline phosphatase activity, respectively. The formation of growth inhibition zones was also observed in the disk diffusion assay for three tested microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. To conclude, the vacuum impregnation method in 45S5 bioglass sol gel solution was effective in penetrating all the interconnected macroporosity of the scaffolds and covering the surface of the struts, which improved their biological properties in vitro, bioactivity and antibacterial activity, without reducing mechanical strength and porosity values. Thus, the β-TCP/45S5 scaffolds are shown as potential candidates for use in tissue engineering, mainly in bone tissue regeneration and recovery. |
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Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation45S5 bioglassAntimicrobial activityBioactivityBiocompatibilityScaffoldsSol-gelTricalcium phosphateIn this work, β-TCP (β-tricalcium phosphate) bioresorbable scaffolds were prepared by the gel casting method. Then, they were impregnated with a 45S5 bioglass sol gel solution to improve biocompatibility and promote bioactivity and antimicrobial activity. The β-TCP scaffolds had an apparent porosity of 72%, and after the incorporation of the bioglass, this porosity was maintained. The elements of the bioglass were incorporated into β-TCP matrix and there was a partial transformation from the β-TCP phase to the α-TCP (α-tricalcium phosphate) phase, besides the formation of bioactive calcium and sodium‑calcium silicates. The scaffolds β-TCP with 45S5 bioglass incorporated (β-TCP/45S5) did not show a reduction in their values of mechanical strength and Weibull modulus, despite the partial transformation to the α-TCP phase. Bioactivity, cell viability, and antimicrobial activity improved significantly for the β-TCP/45S5 scaffold comparing to the scaffold without the bioglass. The mineralization of carbonated hydroxyapatite was verified in Simulated Body Fluid (SBF). The cell viability, evaluated by the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide – MTT in MG63 cells, increased by 178%, and β-TCP/45S5 scaffold also enhanced cell activity and osteoblast differentiation observed by means of total protein contend and alkaline phosphatase activity, respectively. The formation of growth inhibition zones was also observed in the disk diffusion assay for three tested microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. To conclude, the vacuum impregnation method in 45S5 bioglass sol gel solution was effective in penetrating all the interconnected macroporosity of the scaffolds and covering the surface of the struts, which improved their biological properties in vitro, bioactivity and antibacterial activity, without reducing mechanical strength and porosity values. Thus, the β-TCP/45S5 scaffolds are shown as potential candidates for use in tissue engineering, mainly in bone tissue regeneration and recovery.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Federal University of São Paulo (UNIFESP), 330 Talim StTechnological Institute of Aeronautics (ITA), 50 Mal. Eduardo Gomes PlazaSão Paulo State University (UNESP) Institute of Science and Technology, 777 Eng. Francisco José Longo AvenueSão Paulo State University (UNESP) Institute of Science and Technology, 777 Eng. Francisco José Longo AvenueFAPESP: 2015/24659-7Universidade de São Paulo (USP)Technological Institute of Aeronautics (ITA)Universidade Estadual Paulista (UNESP)Spirandeli, B. R.Ribas, R. G.Amaral, S. S. [UNESP]Martins, E. F.Esposito, E.Vasconcellos, L. M.R. [UNESP]Campos, T. M.B.Thim, G. P.Trichês, E. S.2022-04-28T19:46:08Z2022-04-28T19:46:08Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.msec.2021.112453Materials Science and Engineering C, v. 131.1873-01910928-4931http://hdl.handle.net/11449/22269410.1016/j.msec.2021.1124532-s2.0-85117565189Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2022-04-28T19:46:08Zoai:repositorio.unesp.br:11449/222694Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:17:52.439485Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation |
title |
Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation |
spellingShingle |
Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation Spirandeli, B. R. 45S5 bioglass Antimicrobial activity Bioactivity Biocompatibility Scaffolds Sol-gel Tricalcium phosphate |
title_short |
Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation |
title_full |
Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation |
title_fullStr |
Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation |
title_full_unstemmed |
Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation |
title_sort |
Incorporation of 45S5 bioglass via sol-gel in β-TCP scaffolds: Bioactivity and antimicrobial activity evaluation |
author |
Spirandeli, B. R. |
author_facet |
Spirandeli, B. R. Ribas, R. G. Amaral, S. S. [UNESP] Martins, E. F. Esposito, E. Vasconcellos, L. M.R. [UNESP] Campos, T. M.B. Thim, G. P. Trichês, E. S. |
author_role |
author |
author2 |
Ribas, R. G. Amaral, S. S. [UNESP] Martins, E. F. Esposito, E. Vasconcellos, L. M.R. [UNESP] Campos, T. M.B. Thim, G. P. Trichês, E. S. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Technological Institute of Aeronautics (ITA) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Spirandeli, B. R. Ribas, R. G. Amaral, S. S. [UNESP] Martins, E. F. Esposito, E. Vasconcellos, L. M.R. [UNESP] Campos, T. M.B. Thim, G. P. Trichês, E. S. |
dc.subject.por.fl_str_mv |
45S5 bioglass Antimicrobial activity Bioactivity Biocompatibility Scaffolds Sol-gel Tricalcium phosphate |
topic |
45S5 bioglass Antimicrobial activity Bioactivity Biocompatibility Scaffolds Sol-gel Tricalcium phosphate |
description |
In this work, β-TCP (β-tricalcium phosphate) bioresorbable scaffolds were prepared by the gel casting method. Then, they were impregnated with a 45S5 bioglass sol gel solution to improve biocompatibility and promote bioactivity and antimicrobial activity. The β-TCP scaffolds had an apparent porosity of 72%, and after the incorporation of the bioglass, this porosity was maintained. The elements of the bioglass were incorporated into β-TCP matrix and there was a partial transformation from the β-TCP phase to the α-TCP (α-tricalcium phosphate) phase, besides the formation of bioactive calcium and sodium‑calcium silicates. The scaffolds β-TCP with 45S5 bioglass incorporated (β-TCP/45S5) did not show a reduction in their values of mechanical strength and Weibull modulus, despite the partial transformation to the α-TCP phase. Bioactivity, cell viability, and antimicrobial activity improved significantly for the β-TCP/45S5 scaffold comparing to the scaffold without the bioglass. The mineralization of carbonated hydroxyapatite was verified in Simulated Body Fluid (SBF). The cell viability, evaluated by the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide – MTT in MG63 cells, increased by 178%, and β-TCP/45S5 scaffold also enhanced cell activity and osteoblast differentiation observed by means of total protein contend and alkaline phosphatase activity, respectively. The formation of growth inhibition zones was also observed in the disk diffusion assay for three tested microorganisms: Staphylococcus aureus, Escherichia coli and Candida albicans. To conclude, the vacuum impregnation method in 45S5 bioglass sol gel solution was effective in penetrating all the interconnected macroporosity of the scaffolds and covering the surface of the struts, which improved their biological properties in vitro, bioactivity and antibacterial activity, without reducing mechanical strength and porosity values. Thus, the β-TCP/45S5 scaffolds are shown as potential candidates for use in tissue engineering, mainly in bone tissue regeneration and recovery. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-04-28T19:46:08Z 2022-04-28T19:46:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.msec.2021.112453 Materials Science and Engineering C, v. 131. 1873-0191 0928-4931 http://hdl.handle.net/11449/222694 10.1016/j.msec.2021.112453 2-s2.0-85117565189 |
url |
http://dx.doi.org/10.1016/j.msec.2021.112453 http://hdl.handle.net/11449/222694 |
identifier_str_mv |
Materials Science and Engineering C, v. 131. 1873-0191 0928-4931 10.1016/j.msec.2021.112453 2-s2.0-85117565189 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials Science and Engineering C |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129048383062016 |