Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1094/PBIOMES-06-19-0028-R http://hdl.handle.net/11449/195351 |
Resumo: | The microbial communities thriving at the root-soil interface have the potential to improve plant growth and sustainable crop production. Yet, how agricultural practices, such as the application of either mineral or organic nitrogen fertilizers, impact on the composition and functions of these communities remains to be fully elucidated. By deploying a two-pronged 16S rRNA gene sequencing and predictive metagenomics approach, we demonstrated that the bacterial microbiota of field-grown tomato (Solarium tycopersicum) plants is the product of a selective process that progressively differentiates between rhizosphere and root microhabitats. This process initiates as early as plants are in a nursery stage and it is then more marked at late developmental stages, in particular at harvest. This selection acts on both the bacterial relative abundances and phylogenetic assignments, with a bias for the enrichment members of the phylum Actinobacteria in the root compartment. Digestate-based and mineral-based nitrogen fertilizers trigger a distinct bacterial enrichment in both rhizosphere and root microhabitats. This compositional diversification mirrors a predicted functional diversification of the root-inhabiting communities, manifested predominantly by the differential enrichment of genes associated to ABC transporters and the two-component system. Together, our data suggest that the microbiota thriving at the tomato root soil interface is modulated by and in responses to the type of nitrogen fertilizer applied to the field. |
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Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plantsdigestatefertilizersmetagenomicsmicrobiotanitrogenrhizosphere and phyllosphererootSolanum lycopersicumyield and crop productivityThe microbial communities thriving at the root-soil interface have the potential to improve plant growth and sustainable crop production. Yet, how agricultural practices, such as the application of either mineral or organic nitrogen fertilizers, impact on the composition and functions of these communities remains to be fully elucidated. By deploying a two-pronged 16S rRNA gene sequencing and predictive metagenomics approach, we demonstrated that the bacterial microbiota of field-grown tomato (Solarium tycopersicum) plants is the product of a selective process that progressively differentiates between rhizosphere and root microhabitats. This process initiates as early as plants are in a nursery stage and it is then more marked at late developmental stages, in particular at harvest. This selection acts on both the bacterial relative abundances and phylogenetic assignments, with a bias for the enrichment members of the phylum Actinobacteria in the root compartment. Digestate-based and mineral-based nitrogen fertilizers trigger a distinct bacterial enrichment in both rhizosphere and root microhabitats. This compositional diversification mirrors a predicted functional diversification of the root-inhabiting communities, manifested predominantly by the differential enrichment of genes associated to ABC transporters and the two-component system. Together, our data suggest that the microbiota thriving at the tomato root soil interface is modulated by and in responses to the type of nitrogen fertilizer applied to the field.GENBACCA project (Regione Emilia Romagna, POR-FESR 2014/2020 GENBACCA Initiative)Royal Society of Edinburgh/Scottish Government Personal Research FellowshipMarie ActionsBiotechnology and Biological Sciences Research Council (BBSRC) iCASE studentshipHorizon 2020 Framework Programme Innovation Action 'CIRCLES' (European Commission)Univ Modena & Reggio Emilia, Ctr BIOGEST SITEIA, Dept Life Sci, Reggio Emilia, ItalyUniv Dundee, Sch Life Sci, Plant Sci, Dundee, ScotlandSao Paulo State Univ, Fac Agr & Vet Sci, Jaboticabal, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Jaboticabal, BrazilBiotechnology and Biological Sciences Research Council (BBSRC) iCASE studentship: BB/M016811/1Horizon 2020 Framework Programme Innovation Action 'CIRCLES' (European Commission): 818290Amer Phytopathological SocUniv Modena & Reggio EmiliaUniv DundeeUniversidade Estadual Paulista (Unesp)Caradonia, FedericaRonga, DomenicoCatellani, MarcelloAzevedo, Cleber Vinicius Giaretta [UNESP]Terrazas, Rodrigo AlegriaRobertson-Albertyn, SengaFrancia, EnricoBulgarelli, Davide2020-12-10T17:31:35Z2020-12-10T17:31:35Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article315-325http://dx.doi.org/10.1094/PBIOMES-06-19-0028-RPhytobiomes Journal. St Paul: Amer Phytopathological Soc, v. 3, n. 4, p. 315-325, 2019.http://hdl.handle.net/11449/19535110.1094/PBIOMES-06-19-0028-RWOS:000530576000007Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhytobiomes Journalinfo:eu-repo/semantics/openAccess2021-10-23T08:05:14Zoai:repositorio.unesp.br:11449/195351Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T08:05:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants |
title |
Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants |
spellingShingle |
Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants Caradonia, Federica digestate fertilizers metagenomics microbiota nitrogen rhizosphere and phyllosphere root Solanum lycopersicum yield and crop productivity |
title_short |
Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants |
title_full |
Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants |
title_fullStr |
Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants |
title_full_unstemmed |
Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants |
title_sort |
Nitrogen Fertilizers Shape the Composition and Predicted Functions of the Microbiota of Field-Grown Tomato Plants |
author |
Caradonia, Federica |
author_facet |
Caradonia, Federica Ronga, Domenico Catellani, Marcello Azevedo, Cleber Vinicius Giaretta [UNESP] Terrazas, Rodrigo Alegria Robertson-Albertyn, Senga Francia, Enrico Bulgarelli, Davide |
author_role |
author |
author2 |
Ronga, Domenico Catellani, Marcello Azevedo, Cleber Vinicius Giaretta [UNESP] Terrazas, Rodrigo Alegria Robertson-Albertyn, Senga Francia, Enrico Bulgarelli, Davide |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Modena & Reggio Emilia Univ Dundee Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Caradonia, Federica Ronga, Domenico Catellani, Marcello Azevedo, Cleber Vinicius Giaretta [UNESP] Terrazas, Rodrigo Alegria Robertson-Albertyn, Senga Francia, Enrico Bulgarelli, Davide |
dc.subject.por.fl_str_mv |
digestate fertilizers metagenomics microbiota nitrogen rhizosphere and phyllosphere root Solanum lycopersicum yield and crop productivity |
topic |
digestate fertilizers metagenomics microbiota nitrogen rhizosphere and phyllosphere root Solanum lycopersicum yield and crop productivity |
description |
The microbial communities thriving at the root-soil interface have the potential to improve plant growth and sustainable crop production. Yet, how agricultural practices, such as the application of either mineral or organic nitrogen fertilizers, impact on the composition and functions of these communities remains to be fully elucidated. By deploying a two-pronged 16S rRNA gene sequencing and predictive metagenomics approach, we demonstrated that the bacterial microbiota of field-grown tomato (Solarium tycopersicum) plants is the product of a selective process that progressively differentiates between rhizosphere and root microhabitats. This process initiates as early as plants are in a nursery stage and it is then more marked at late developmental stages, in particular at harvest. This selection acts on both the bacterial relative abundances and phylogenetic assignments, with a bias for the enrichment members of the phylum Actinobacteria in the root compartment. Digestate-based and mineral-based nitrogen fertilizers trigger a distinct bacterial enrichment in both rhizosphere and root microhabitats. This compositional diversification mirrors a predicted functional diversification of the root-inhabiting communities, manifested predominantly by the differential enrichment of genes associated to ABC transporters and the two-component system. Together, our data suggest that the microbiota thriving at the tomato root soil interface is modulated by and in responses to the type of nitrogen fertilizer applied to the field. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 2020-12-10T17:31:35Z 2020-12-10T17:31:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1094/PBIOMES-06-19-0028-R Phytobiomes Journal. St Paul: Amer Phytopathological Soc, v. 3, n. 4, p. 315-325, 2019. http://hdl.handle.net/11449/195351 10.1094/PBIOMES-06-19-0028-R WOS:000530576000007 |
url |
http://dx.doi.org/10.1094/PBIOMES-06-19-0028-R http://hdl.handle.net/11449/195351 |
identifier_str_mv |
Phytobiomes Journal. St Paul: Amer Phytopathological Soc, v. 3, n. 4, p. 315-325, 2019. 10.1094/PBIOMES-06-19-0028-R WOS:000530576000007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Phytobiomes Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
315-325 |
dc.publisher.none.fl_str_mv |
Amer Phytopathological Soc |
publisher.none.fl_str_mv |
Amer Phytopathological Soc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790247036125184 |