Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jcrs.2014.11.047 http://hdl.handle.net/11449/160783 |
Resumo: | PURPOSE: To evaluate the adhesion of human lens capsule cells on hydrophilic acrylic intraocular lenses (IOLs) coated with polyethylene glycol (PEG). SETTING: Department of Ophthalmology, Faculty of Medicine, Universidade Estadual Paulista Botucatu, Sao Paulo, Brazil. DESIGN: Experimental study. METHODS: Human anterior lens capsules obtained during cataract surgery were cultured and seeded (200 cells/IOLs) in triplicates on the surface of a copolymer comprising hydroxyethyl methacrylate, ethyl methacrylate, and methyl methacrylate IOLs (Loflex) treated or not treated with PEG. After 26 hours, the number of viable adherent cells was estimated by counting in a hemocytometer. RESULTS: The coating of hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion (P < .05). Cells showing 2 distinct morphologic patterns epithelial and dendritic-like-were observed during the in vitro establishment of the cultures. A tendency toward greater adhesion of dendritic-like cells was observed in untreated IOLs compared with treated IOLs (P = .095). CONCLUSION: Coating hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion. This treatment might play a role in posterior capsule opacification prevention. (C) ASCRS and ESCRS |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycolPURPOSE: To evaluate the adhesion of human lens capsule cells on hydrophilic acrylic intraocular lenses (IOLs) coated with polyethylene glycol (PEG). SETTING: Department of Ophthalmology, Faculty of Medicine, Universidade Estadual Paulista Botucatu, Sao Paulo, Brazil. DESIGN: Experimental study. METHODS: Human anterior lens capsules obtained during cataract surgery were cultured and seeded (200 cells/IOLs) in triplicates on the surface of a copolymer comprising hydroxyethyl methacrylate, ethyl methacrylate, and methyl methacrylate IOLs (Loflex) treated or not treated with PEG. After 26 hours, the number of viable adherent cells was estimated by counting in a hemocytometer. RESULTS: The coating of hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion (P < .05). Cells showing 2 distinct morphologic patterns epithelial and dendritic-like-were observed during the in vitro establishment of the cultures. A tendency toward greater adhesion of dendritic-like cells was observed in untreated IOLs compared with treated IOLs (P = .095). CONCLUSION: Coating hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion. This treatment might play a role in posterior capsule opacification prevention. (C) ASCRS and ESCRSUniv Estadual Paulista, Grad Program Gen Basis Surg, Biosci Inst, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Biosci Inst, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Genet, Biosci Inst, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Biostat, Biosci Inst, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Ophthalmol Otorhinolaryngol & Head & Neck Su, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Control & Automat Engn, Sao Paulo, BrazilUniv Estadual Paulista, Grad Program Gen Basis Surg, Biosci Inst, Botucatu, SP, BrazilUniv Estadual Paulista, Botucatu Med Sch, Biosci Inst, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Genet, Biosci Inst, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Biostat, Biosci Inst, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Ophthalmol Otorhinolaryngol & Head & Neck Su, Botucatu, SP, BrazilUniv Estadual Paulista, Dept Control & Automat Engn, Sao Paulo, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Hata Viveiros, Magda Massae [UNESP]Soares, Ricardo Torres [UNESP]Model, Michelle Sako [UNESP]Rainho, Claudia Aparecida [UNESP]Padovani, Carlos Roberto [UNESP]Cruz, Nilson [UNESP]Schellini, Silvana Artioli [UNESP]Lottelli Rodrigues, Antonio Carlos [UNESP]2018-11-26T16:16:43Z2018-11-26T16:16:43Z2015-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1478-1483application/pdfhttp://dx.doi.org/10.1016/j.jcrs.2014.11.047Journal Of Cataract And Refractive Surgery. New York: Elsevier Science Inc, v. 41, n. 7, p. 1478-1483, 2015.0886-3350http://hdl.handle.net/11449/16078310.1016/j.jcrs.2014.11.047WOS:000360871600022WOS000360871600022.pdf88148235451595040000-0002-0285-1162Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Cataract And Refractive Surgery1,799info:eu-repo/semantics/openAccess2023-10-03T06:01:10Zoai:repositorio.unesp.br:11449/160783Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-03T06:01:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol |
title |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol |
spellingShingle |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol Hata Viveiros, Magda Massae [UNESP] |
title_short |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol |
title_full |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol |
title_fullStr |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol |
title_full_unstemmed |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol |
title_sort |
Adhesion study of cultured human lens capsule cells on hydrophilic intraocular lenses coated with polyethylene glycol |
author |
Hata Viveiros, Magda Massae [UNESP] |
author_facet |
Hata Viveiros, Magda Massae [UNESP] Soares, Ricardo Torres [UNESP] Model, Michelle Sako [UNESP] Rainho, Claudia Aparecida [UNESP] Padovani, Carlos Roberto [UNESP] Cruz, Nilson [UNESP] Schellini, Silvana Artioli [UNESP] Lottelli Rodrigues, Antonio Carlos [UNESP] |
author_role |
author |
author2 |
Soares, Ricardo Torres [UNESP] Model, Michelle Sako [UNESP] Rainho, Claudia Aparecida [UNESP] Padovani, Carlos Roberto [UNESP] Cruz, Nilson [UNESP] Schellini, Silvana Artioli [UNESP] Lottelli Rodrigues, Antonio Carlos [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Hata Viveiros, Magda Massae [UNESP] Soares, Ricardo Torres [UNESP] Model, Michelle Sako [UNESP] Rainho, Claudia Aparecida [UNESP] Padovani, Carlos Roberto [UNESP] Cruz, Nilson [UNESP] Schellini, Silvana Artioli [UNESP] Lottelli Rodrigues, Antonio Carlos [UNESP] |
description |
PURPOSE: To evaluate the adhesion of human lens capsule cells on hydrophilic acrylic intraocular lenses (IOLs) coated with polyethylene glycol (PEG). SETTING: Department of Ophthalmology, Faculty of Medicine, Universidade Estadual Paulista Botucatu, Sao Paulo, Brazil. DESIGN: Experimental study. METHODS: Human anterior lens capsules obtained during cataract surgery were cultured and seeded (200 cells/IOLs) in triplicates on the surface of a copolymer comprising hydroxyethyl methacrylate, ethyl methacrylate, and methyl methacrylate IOLs (Loflex) treated or not treated with PEG. After 26 hours, the number of viable adherent cells was estimated by counting in a hemocytometer. RESULTS: The coating of hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion (P < .05). Cells showing 2 distinct morphologic patterns epithelial and dendritic-like-were observed during the in vitro establishment of the cultures. A tendency toward greater adhesion of dendritic-like cells was observed in untreated IOLs compared with treated IOLs (P = .095). CONCLUSION: Coating hydrophilic acrylic IOLs with PEG was effective in inhibiting cell adhesion. This treatment might play a role in posterior capsule opacification prevention. (C) ASCRS and ESCRS |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-01 2018-11-26T16:16:43Z 2018-11-26T16:16:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jcrs.2014.11.047 Journal Of Cataract And Refractive Surgery. New York: Elsevier Science Inc, v. 41, n. 7, p. 1478-1483, 2015. 0886-3350 http://hdl.handle.net/11449/160783 10.1016/j.jcrs.2014.11.047 WOS:000360871600022 WOS000360871600022.pdf 8814823545159504 0000-0002-0285-1162 |
url |
http://dx.doi.org/10.1016/j.jcrs.2014.11.047 http://hdl.handle.net/11449/160783 |
identifier_str_mv |
Journal Of Cataract And Refractive Surgery. New York: Elsevier Science Inc, v. 41, n. 7, p. 1478-1483, 2015. 0886-3350 10.1016/j.jcrs.2014.11.047 WOS:000360871600022 WOS000360871600022.pdf 8814823545159504 0000-0002-0285-1162 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Cataract And Refractive Surgery 1,799 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1478-1483 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964410772455424 |