Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jnutbio.2017.08.006 http://hdl.handle.net/11449/170210 |
Resumo: | Obesity and type 2 diabetes are characterized by subclinical inflammatory process. Changes in composition or modulation of the gut microbiota may play an important role in the obesity-associated inflammatory process. In the current study, we evaluated the effects of probiotics (Lactobacillus rhamnosus, L. acidophilus and Bifidobacterium bifidumi) on gut microbiota, changes in permeability, and insulin sensitivity and signaling in high-fat diet and control animals. More importantly, we investigated the effects of these gut modulations on hypothalamic control of food intake, and insulin and leptin signaling. Swiss mice were submitted to a high-fat diet (HFD) with probiotics or pair-feeding for 5 weeks. Metagenome analyses were performed on DNA samples from mouse feces. Blood was drawn to determine levels of glucose, insulin, LPS, cytokines and GLP-1. Liver, muscle, ileum and hypothalamus tissue proteins were analyzed by Western blotting and real-time polymerase chain reaction. In addition, liver and adipose tissues were analyzed using histology and immunohistochemistry. The HFD induced huge alterations in gut microbiota accompanied by increased intestinal permeability, LPS translocation and systemic low-grade inflammation, resulting in decreased glucose tolerance and hyperphagic behavior. All these obesity-related features were reversed by changes in the gut microbiota profile induced by probiotics. Probiotics also induced an improvement in hypothalamic insulin and leptin resistance. Our data demonstrate that the intestinal microbiome is a key modulator of inflammatory and metabolic pathways in both peripheral and central tissues. These findings shed light on probiotics as an important tool to prevent and treat patients with obesity and insulin resistance. |
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Probiotics modulate gut microbiota and improve insulin sensitivity in DIO miceGut microbiotaInsulin sensitivityProbioticsObesity and type 2 diabetes are characterized by subclinical inflammatory process. Changes in composition or modulation of the gut microbiota may play an important role in the obesity-associated inflammatory process. In the current study, we evaluated the effects of probiotics (Lactobacillus rhamnosus, L. acidophilus and Bifidobacterium bifidumi) on gut microbiota, changes in permeability, and insulin sensitivity and signaling in high-fat diet and control animals. More importantly, we investigated the effects of these gut modulations on hypothalamic control of food intake, and insulin and leptin signaling. Swiss mice were submitted to a high-fat diet (HFD) with probiotics or pair-feeding for 5 weeks. Metagenome analyses were performed on DNA samples from mouse feces. Blood was drawn to determine levels of glucose, insulin, LPS, cytokines and GLP-1. Liver, muscle, ileum and hypothalamus tissue proteins were analyzed by Western blotting and real-time polymerase chain reaction. In addition, liver and adipose tissues were analyzed using histology and immunohistochemistry. The HFD induced huge alterations in gut microbiota accompanied by increased intestinal permeability, LPS translocation and systemic low-grade inflammation, resulting in decreased glucose tolerance and hyperphagic behavior. All these obesity-related features were reversed by changes in the gut microbiota profile induced by probiotics. Probiotics also induced an improvement in hypothalamic insulin and leptin resistance. Our data demonstrate that the intestinal microbiome is a key modulator of inflammatory and metabolic pathways in both peripheral and central tissues. These findings shed light on probiotics as an important tool to prevent and treat patients with obesity and insulin resistance.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Instituto Nacional de Ciência e Tecnologia de Obesidade e DiabetesDepartment of Internal Medicine State University of CampinasDepartment of Physical Education São Paulo State University (UNESP) Bioscience InstituteDepartment of Biology Science Federal University of PernambucoDepartment of Physical Education São Paulo State University (UNESP) Bioscience InstituteFAPESP: 2012/15009-0Instituto Nacional de Ciência e Tecnologia de Obesidade e Diabetes: 573856/2008-7Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Universidade Federal de Pernambuco (UFPE)Bagarolli, Renata A.Tobar, NatáliaOliveira, Alexandre G. [UNESP]Araújo, Tiago G.Carvalho, Bruno M.Rocha, Guilherme Z.Vecina, Juliana F.Calisto, KellyGuadagnini, DiozePrada, Patrícia O.Santos, AndreySaad, Sara T.O.Saad, Mario J.A.2018-12-11T16:49:46Z2018-12-11T16:49:46Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16-25application/pdfhttp://dx.doi.org/10.1016/j.jnutbio.2017.08.006Journal of Nutritional Biochemistry, v. 50, p. 16-25.1873-48470955-2863http://hdl.handle.net/11449/17021010.1016/j.jnutbio.2017.08.0062-s2.0-850300945372-s2.0-85030094537.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Nutritional Biochemistry1,678info:eu-repo/semantics/openAccess2023-11-15T06:13:31Zoai:repositorio.unesp.br:11449/170210Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-15T06:13:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice |
title |
Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice |
spellingShingle |
Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice Bagarolli, Renata A. Gut microbiota Insulin sensitivity Probiotics |
title_short |
Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice |
title_full |
Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice |
title_fullStr |
Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice |
title_full_unstemmed |
Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice |
title_sort |
Probiotics modulate gut microbiota and improve insulin sensitivity in DIO mice |
author |
Bagarolli, Renata A. |
author_facet |
Bagarolli, Renata A. Tobar, Natália Oliveira, Alexandre G. [UNESP] Araújo, Tiago G. Carvalho, Bruno M. Rocha, Guilherme Z. Vecina, Juliana F. Calisto, Kelly Guadagnini, Dioze Prada, Patrícia O. Santos, Andrey Saad, Sara T.O. Saad, Mario J.A. |
author_role |
author |
author2 |
Tobar, Natália Oliveira, Alexandre G. [UNESP] Araújo, Tiago G. Carvalho, Bruno M. Rocha, Guilherme Z. Vecina, Juliana F. Calisto, Kelly Guadagnini, Dioze Prada, Patrícia O. Santos, Andrey Saad, Sara T.O. Saad, Mario J.A. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Universidade Federal de Pernambuco (UFPE) |
dc.contributor.author.fl_str_mv |
Bagarolli, Renata A. Tobar, Natália Oliveira, Alexandre G. [UNESP] Araújo, Tiago G. Carvalho, Bruno M. Rocha, Guilherme Z. Vecina, Juliana F. Calisto, Kelly Guadagnini, Dioze Prada, Patrícia O. Santos, Andrey Saad, Sara T.O. Saad, Mario J.A. |
dc.subject.por.fl_str_mv |
Gut microbiota Insulin sensitivity Probiotics |
topic |
Gut microbiota Insulin sensitivity Probiotics |
description |
Obesity and type 2 diabetes are characterized by subclinical inflammatory process. Changes in composition or modulation of the gut microbiota may play an important role in the obesity-associated inflammatory process. In the current study, we evaluated the effects of probiotics (Lactobacillus rhamnosus, L. acidophilus and Bifidobacterium bifidumi) on gut microbiota, changes in permeability, and insulin sensitivity and signaling in high-fat diet and control animals. More importantly, we investigated the effects of these gut modulations on hypothalamic control of food intake, and insulin and leptin signaling. Swiss mice were submitted to a high-fat diet (HFD) with probiotics or pair-feeding for 5 weeks. Metagenome analyses were performed on DNA samples from mouse feces. Blood was drawn to determine levels of glucose, insulin, LPS, cytokines and GLP-1. Liver, muscle, ileum and hypothalamus tissue proteins were analyzed by Western blotting and real-time polymerase chain reaction. In addition, liver and adipose tissues were analyzed using histology and immunohistochemistry. The HFD induced huge alterations in gut microbiota accompanied by increased intestinal permeability, LPS translocation and systemic low-grade inflammation, resulting in decreased glucose tolerance and hyperphagic behavior. All these obesity-related features were reversed by changes in the gut microbiota profile induced by probiotics. Probiotics also induced an improvement in hypothalamic insulin and leptin resistance. Our data demonstrate that the intestinal microbiome is a key modulator of inflammatory and metabolic pathways in both peripheral and central tissues. These findings shed light on probiotics as an important tool to prevent and treat patients with obesity and insulin resistance. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-12-11T16:49:46Z 2018-12-11T16:49:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jnutbio.2017.08.006 Journal of Nutritional Biochemistry, v. 50, p. 16-25. 1873-4847 0955-2863 http://hdl.handle.net/11449/170210 10.1016/j.jnutbio.2017.08.006 2-s2.0-85030094537 2-s2.0-85030094537.pdf |
url |
http://dx.doi.org/10.1016/j.jnutbio.2017.08.006 http://hdl.handle.net/11449/170210 |
identifier_str_mv |
Journal of Nutritional Biochemistry, v. 50, p. 16-25. 1873-4847 0955-2863 10.1016/j.jnutbio.2017.08.006 2-s2.0-85030094537 2-s2.0-85030094537.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Nutritional Biochemistry 1,678 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16-25 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964943286534144 |