Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches

Detalhes bibliográficos
Autor(a) principal: Feliciano, M.a.r.
Data de Publicação: 2014
Outros Autores: Silva, A.s.l., Crivelaro, R.m., Oliveira, M.e.f., Coutinho, L.n., Vicente, W.r.r.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-6392
http://hdl.handle.net/11449/114175
Resumo: The aim of this study was to determine the profile of IL-2, IL-4, IL-10, IFN-γ, and TNF-α cytokines and KC-like cells (natural killer) in pregnant bitches, unpublished values for the species. A total of 27 females of the Shi Tzu, Pug, English Bulldog and French breeds, weighing 4-20kg and aged 4-6 years were used. Blood samples were collected from bitches during the anestrous and on the 2nd, 5th, 6th, 7th and 8th week of pregnancy. Serum levels of cytokines were measured by panel MILLIPLEX MAP (CCYTO-90K, MILLIPORE, Billerica, Massachusetts, USA) validated for dogs. Twenty four females showed physiological pregnancy and three bitches showed pathological pregnancy. There was no difference between cytokine values during anestrous and gestational weeks of bitches (P>0.05). However, it was possible to verify the physiological behavior of serum levels during modulation of immune response in the gestational process of animals. In animals with gestational disorders, abnormal values for IL-2, IL-4 and INF-y were noted. It was concluded that serum levels of cytokines evaluated in pregnant bitches can help the better understanding of physiological and pathological gestational processes and correlated immunology in this species.
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spelling Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitchesPerfil de citocinas IL-2, IL-4, IL-10, IFN-?, TNF-? e células KC-like em cadelas gestantescãoimunidadegestaçãodogimmunitypregnancyThe aim of this study was to determine the profile of IL-2, IL-4, IL-10, IFN-γ, and TNF-α cytokines and KC-like cells (natural killer) in pregnant bitches, unpublished values for the species. A total of 27 females of the Shi Tzu, Pug, English Bulldog and French breeds, weighing 4-20kg and aged 4-6 years were used. Blood samples were collected from bitches during the anestrous and on the 2nd, 5th, 6th, 7th and 8th week of pregnancy. Serum levels of cytokines were measured by panel MILLIPLEX MAP (CCYTO-90K, MILLIPORE, Billerica, Massachusetts, USA) validated for dogs. Twenty four females showed physiological pregnancy and three bitches showed pathological pregnancy. There was no difference between cytokine values during anestrous and gestational weeks of bitches (P>0.05). However, it was possible to verify the physiological behavior of serum levels during modulation of immune response in the gestational process of animals. In animals with gestational disorders, abnormal values for IL-2, IL-4 and INF-y were noted. It was concluded that serum levels of cytokines evaluated in pregnant bitches can help the better understanding of physiological and pathological gestational processes and correlated immunology in this species.Objetivou-se por meio deste estudo determinar o perfil das citocinas IL-2, IL-4, IL-10, IFN-γ, TNF-α e células KC-like (natural killer) em cadelas gestantes, valores que são inéditos para a espécie. Foram utilizadas 27 fêmeas das raças Shi-tzu, Pug, Bulldog Inglês e Francês, pesando de 4 a 20 kg e com idade entre quatro e seis anos. Foram coletadas amostras sanguíneas das cadelas durante o anestro e na segunda, quinta, sexta, sétima e oitava semanas gestacionais. Os níveis séricos de citocinas foram dosados pelo painel MILLIPLEX MAP (CCYTO-90K, MILLIPORE, Billerica, Massachusetts, EUA) validado para cães. Vinte e quatro fêmeas apresentaram gestação fisiológica e três cadelas, gestação patológica. Não foi verificada diferença entre os valores das citocinas durante anestro e nas semanas gestacionais das cadelas (P>0,05). Entretanto, foi possível verificar o comportamento fisiológico dos níveis séricos durante a modulação da resposta imune no processo gestacional dos animais. Verificaram-se, nos animais com afecções gestacionais, valores alterados para IL-2, IL-4 e INF-y. Pôde-se concluir que os valores séricos das citocinas avaliadas, nas cadelas gestantes, podem auxiliar no maior conhecimento dos processos fisiológicos e patológicos da gestação, correlacionados à imunologia, nessa espécie.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Unesp Faculdade de Ciências Agrárias e VeterináriasUnesp Faculdade de Ciências Agrárias e VeterináriasUniversidade Federal de Minas Gerais, Escola de VeterináriaUniversidade Estadual Paulista (Unesp)Feliciano, M.a.r.Silva, A.s.l.Crivelaro, R.m.Oliveira, M.e.f.Coutinho, L.n.Vicente, W.r.r.2015-02-02T12:39:18Z2015-02-02T12:39:18Z2014-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1067-1072application/pdfhttp://dx.doi.org/10.1590/1678-6392Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 66, n. 4, p. 1067-1072, 2014.0102-0935http://hdl.handle.net/11449/11417510.1590/1678-6392S0102-09352014000401067S0102-09352014000401067.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinária e Zootecnia0.2860,248info:eu-repo/semantics/openAccess2023-11-11T06:08:24Zoai:repositorio.unesp.br:11449/114175Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-11T06:08:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches
Perfil de citocinas IL-2, IL-4, IL-10, IFN-?, TNF-? e células KC-like em cadelas gestantes
title Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches
spellingShingle Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches
Feliciano, M.a.r.
cão
imunidade
gestação
dog
immunity
pregnancy
title_short Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches
title_full Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches
title_fullStr Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches
title_full_unstemmed Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches
title_sort Profile of IL-2, IL-4, IL-10, IFN- ? , TNF- ? and KC-like cytokines in pregnant bitches
author Feliciano, M.a.r.
author_facet Feliciano, M.a.r.
Silva, A.s.l.
Crivelaro, R.m.
Oliveira, M.e.f.
Coutinho, L.n.
Vicente, W.r.r.
author_role author
author2 Silva, A.s.l.
Crivelaro, R.m.
Oliveira, M.e.f.
Coutinho, L.n.
Vicente, W.r.r.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Feliciano, M.a.r.
Silva, A.s.l.
Crivelaro, R.m.
Oliveira, M.e.f.
Coutinho, L.n.
Vicente, W.r.r.
dc.subject.por.fl_str_mv cão
imunidade
gestação
dog
immunity
pregnancy
topic cão
imunidade
gestação
dog
immunity
pregnancy
description The aim of this study was to determine the profile of IL-2, IL-4, IL-10, IFN-γ, and TNF-α cytokines and KC-like cells (natural killer) in pregnant bitches, unpublished values for the species. A total of 27 females of the Shi Tzu, Pug, English Bulldog and French breeds, weighing 4-20kg and aged 4-6 years were used. Blood samples were collected from bitches during the anestrous and on the 2nd, 5th, 6th, 7th and 8th week of pregnancy. Serum levels of cytokines were measured by panel MILLIPLEX MAP (CCYTO-90K, MILLIPORE, Billerica, Massachusetts, USA) validated for dogs. Twenty four females showed physiological pregnancy and three bitches showed pathological pregnancy. There was no difference between cytokine values during anestrous and gestational weeks of bitches (P>0.05). However, it was possible to verify the physiological behavior of serum levels during modulation of immune response in the gestational process of animals. In animals with gestational disorders, abnormal values for IL-2, IL-4 and INF-y were noted. It was concluded that serum levels of cytokines evaluated in pregnant bitches can help the better understanding of physiological and pathological gestational processes and correlated immunology in this species.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-01
2015-02-02T12:39:18Z
2015-02-02T12:39:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-6392
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 66, n. 4, p. 1067-1072, 2014.
0102-0935
http://hdl.handle.net/11449/114175
10.1590/1678-6392
S0102-09352014000401067
S0102-09352014000401067.pdf
url http://dx.doi.org/10.1590/1678-6392
http://hdl.handle.net/11449/114175
identifier_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 66, n. 4, p. 1067-1072, 2014.
0102-0935
10.1590/1678-6392
S0102-09352014000401067
S0102-09352014000401067.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia
0.286
0,248
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1067-1072
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964889809158144

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