Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer

Detalhes bibliográficos
Autor(a) principal: da Conceição, Poliana
Data de Publicação: 2023
Outros Autores: dos Santos Neto, Antonio Gomes, Khan, Sabir [UNESP], Tanaka, Auro A., Santana, Antônio Euzébio G., del Pilar Taboada-Sotomayor, Maria [UNESP], Goulart, Marília O. F., Santos, Ana Caroline Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00604-023-05738-7
http://hdl.handle.net/11449/247052
Resumo: A magnetic graphite-epoxy composite (m-GEC) electrochemical sensor is presented based on magnetic imprinted polymer (mag-MIP) to determine homocysteine (Hcy). Mag-MIP was synthesized via precipitation polymerization, using functionalized magnetic nanoparticles (Fe3O4) together with the template molecule (Hcy), the functional monomer 2-hydroxyethyl methacrylate (HEMA), and the structural monomer trimethylolpropane trimethacrylate (TRIM). For mag-NIP (magnetic non-imprinted polymer), the procedure was the same in the absence of Hcy. Morphological and structural properties of the resultant mag-MIP and mag-NIP were examined using TEM, FT-IR, and Vibrating Sample Magnetometer. Under optimized conditions, the m-GEC/mag-MIP sensor showed a linear range of 0.1–2 µmol L−1, with a limit of detection (LOD) of 0.030 µmol L−1. In addition, the proposed sensor responded selectively to Hcy compared to several interferents present in biological samples. The recovery values determined by differential pulse voltammetry (DPV) were close to 100% for natural and synthetic samples, indicating good method accuracy. The developed electrochemical sensor proves to be a suitable device for determining Hcy, with advantages related to magnetic separation and electrochemical analysis.
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spelling Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymerDifferential pulse voltammetryElectroanalysisElectrochemical sensorFe3O4 nanoparticlesHyperhomocysteinemiaMagnetic molecularly imprinted polymerA magnetic graphite-epoxy composite (m-GEC) electrochemical sensor is presented based on magnetic imprinted polymer (mag-MIP) to determine homocysteine (Hcy). Mag-MIP was synthesized via precipitation polymerization, using functionalized magnetic nanoparticles (Fe3O4) together with the template molecule (Hcy), the functional monomer 2-hydroxyethyl methacrylate (HEMA), and the structural monomer trimethylolpropane trimethacrylate (TRIM). For mag-NIP (magnetic non-imprinted polymer), the procedure was the same in the absence of Hcy. Morphological and structural properties of the resultant mag-MIP and mag-NIP were examined using TEM, FT-IR, and Vibrating Sample Magnetometer. Under optimized conditions, the m-GEC/mag-MIP sensor showed a linear range of 0.1–2 µmol L−1, with a limit of detection (LOD) of 0.030 µmol L−1. In addition, the proposed sensor responded selectively to Hcy compared to several interferents present in biological samples. The recovery values determined by differential pulse voltammetry (DPV) were close to 100% for natural and synthetic samples, indicating good method accuracy. The developed electrochemical sensor proves to be a suitable device for determining Hcy, with advantages related to magnetic separation and electrochemical analysis.Programa de Pós-graduação em Química e Biotecnologia (PPGQB) Instituto de Química e Biotecnologia Universidade Federal de Alagoas, Campus A.C. Simões, Tabuleiro dos Martins, ALDepartamento de Química Universidade Federal do Maranhão Avenida dos Portugueses, MAInstituto de Química INCT-DATREM Universidade Estadual Paulista, Rua Prof. Francisco Degni, 55, SPNational Institute of Science and Technology of Bioanalytics (INCT-Bio)Instituto de Química INCT-DATREM Universidade Estadual Paulista, Rua Prof. Francisco Degni, 55, SPUniversidade Federal de AlagoasAvenida dos PortuguesesUniversidade Estadual Paulista (UNESP)National Institute of Science and Technology of Bioanalytics (INCT-Bio)da Conceição, Polianados Santos Neto, Antonio GomesKhan, Sabir [UNESP]Tanaka, Auro A.Santana, Antônio Euzébio G.del Pilar Taboada-Sotomayor, Maria [UNESP]Goulart, Marília O. F.Santos, Ana Caroline Ferreira2023-07-29T12:57:42Z2023-07-29T12:57:42Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00604-023-05738-7Microchimica Acta, v. 190, n. 4, 2023.1436-50730026-3672http://hdl.handle.net/11449/24705210.1007/s00604-023-05738-72-s2.0-85150956061Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrochimica Actainfo:eu-repo/semantics/openAccess2023-07-29T12:57:42Zoai:repositorio.unesp.br:11449/247052Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:57:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer
title Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer
spellingShingle Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer
da Conceição, Poliana
Differential pulse voltammetry
Electroanalysis
Electrochemical sensor
Fe3O4 nanoparticles
Hyperhomocysteinemia
Magnetic molecularly imprinted polymer
title_short Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer
title_full Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer
title_fullStr Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer
title_full_unstemmed Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer
title_sort Extraction-assisted voltammetric determination of homocysteine using magnetic nanoparticles modified with molecularly imprinted polymer
author da Conceição, Poliana
author_facet da Conceição, Poliana
dos Santos Neto, Antonio Gomes
Khan, Sabir [UNESP]
Tanaka, Auro A.
Santana, Antônio Euzébio G.
del Pilar Taboada-Sotomayor, Maria [UNESP]
Goulart, Marília O. F.
Santos, Ana Caroline Ferreira
author_role author
author2 dos Santos Neto, Antonio Gomes
Khan, Sabir [UNESP]
Tanaka, Auro A.
Santana, Antônio Euzébio G.
del Pilar Taboada-Sotomayor, Maria [UNESP]
Goulart, Marília O. F.
Santos, Ana Caroline Ferreira
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Alagoas
Avenida dos Portugueses
Universidade Estadual Paulista (UNESP)
National Institute of Science and Technology of Bioanalytics (INCT-Bio)
dc.contributor.author.fl_str_mv da Conceição, Poliana
dos Santos Neto, Antonio Gomes
Khan, Sabir [UNESP]
Tanaka, Auro A.
Santana, Antônio Euzébio G.
del Pilar Taboada-Sotomayor, Maria [UNESP]
Goulart, Marília O. F.
Santos, Ana Caroline Ferreira
dc.subject.por.fl_str_mv Differential pulse voltammetry
Electroanalysis
Electrochemical sensor
Fe3O4 nanoparticles
Hyperhomocysteinemia
Magnetic molecularly imprinted polymer
topic Differential pulse voltammetry
Electroanalysis
Electrochemical sensor
Fe3O4 nanoparticles
Hyperhomocysteinemia
Magnetic molecularly imprinted polymer
description A magnetic graphite-epoxy composite (m-GEC) electrochemical sensor is presented based on magnetic imprinted polymer (mag-MIP) to determine homocysteine (Hcy). Mag-MIP was synthesized via precipitation polymerization, using functionalized magnetic nanoparticles (Fe3O4) together with the template molecule (Hcy), the functional monomer 2-hydroxyethyl methacrylate (HEMA), and the structural monomer trimethylolpropane trimethacrylate (TRIM). For mag-NIP (magnetic non-imprinted polymer), the procedure was the same in the absence of Hcy. Morphological and structural properties of the resultant mag-MIP and mag-NIP were examined using TEM, FT-IR, and Vibrating Sample Magnetometer. Under optimized conditions, the m-GEC/mag-MIP sensor showed a linear range of 0.1–2 µmol L−1, with a limit of detection (LOD) of 0.030 µmol L−1. In addition, the proposed sensor responded selectively to Hcy compared to several interferents present in biological samples. The recovery values determined by differential pulse voltammetry (DPV) were close to 100% for natural and synthetic samples, indicating good method accuracy. The developed electrochemical sensor proves to be a suitable device for determining Hcy, with advantages related to magnetic separation and electrochemical analysis.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T12:57:42Z
2023-07-29T12:57:42Z
2023-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00604-023-05738-7
Microchimica Acta, v. 190, n. 4, 2023.
1436-5073
0026-3672
http://hdl.handle.net/11449/247052
10.1007/s00604-023-05738-7
2-s2.0-85150956061
url http://dx.doi.org/10.1007/s00604-023-05738-7
http://hdl.handle.net/11449/247052
identifier_str_mv Microchimica Acta, v. 190, n. 4, 2023.
1436-5073
0026-3672
10.1007/s00604-023-05738-7
2-s2.0-85150956061
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microchimica Acta
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1797789806562902016

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