Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0037-8682-0183-2014 http://hdl.handle.net/11449/114114 |
Resumo: | Introduction Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar. Methods To determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification. Results The nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival. Conclusions NAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis. |
id |
UNSP_8ec99af1e620e70352753878d8781db2 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/114114 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantumGenetic diversityKala-azarVisceral leishmaniasisMacrophage inhibition factorTropical diseasesSingle nucleotide polymorphismIntroduction Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar. Methods To determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification. Results The nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival. Conclusions NAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal do Piauí Instituto de Doenças Tropicais Natan Portella Laboratório de LeishmaniosesUniversidade Federal de Juiz de Fora Laboratório de VirologiaUniversidade Federal de Alfenas Laboratório de VacinasUniversidade Federal da Paraíba Sociais e Agrárias Centro de Ciências HumanasUniversidade Estadual Paulista Instituto de Biociências Departamento de ParasitologiaUniversidade Federal do Pará Núcleo de Medicina Tropical Laboratório de Biologia MolecularUniversidade Estadual Paulista Instituto de Biociências Departamento de ParasitologiaSociedade Brasileira de Medicina Tropical - SBMTUniversidade Federal do Piauí (UFPI)Universidade Federal de Juiz de Fora (UFJF)Universidade Federal de Alfenas (UNIFAL)Universidade Federal da Paraíba (UFPB)Universidade Estadual Paulista (Unesp)Universidade Federal do Pará Núcleo de Medicina Tropical Laboratório de Biologia MolecularAguiar, Bruno Guedes AlcoforadoCoelho, Daniela LemosCosta, Dorcas LamounierDrumond, Betânia PaivaCoelho, Luiz Felipe LeomilFigueiredo, Lívio CarvalhoZacarias, Danielle AlvesSilva, Jailthon Carlos DaAlonso, Diego Peres [UNESP]Ribolla, Paulo Eduardo MartinsIshikawa, Edna Aoba YassuiGaído, Samara BelchiorCosta, Carlos Henrique Nery2015-02-02T12:39:13Z2015-02-02T12:39:13Z2014-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article593-598application/pdfhttp://dx.doi.org/10.1590/0037-8682-0183-2014Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 47, n. 5, p. 593-598, 2014.0037-8682http://hdl.handle.net/11449/11411410.1590/0037-8682-0183-2014S0037-86822014000500593S0037-86822014000500593.pdf35771497484568800000-0001-8735-6090SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropical1.3580,658info:eu-repo/semantics/openAccess2023-12-17T06:21:34Zoai:repositorio.unesp.br:11449/114114Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-17T06:21:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
spellingShingle |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum Aguiar, Bruno Guedes Alcoforado Genetic diversity Kala-azar Visceral leishmaniasis Macrophage inhibition factor Tropical diseases Single nucleotide polymorphism |
title_short |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_full |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_fullStr |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_full_unstemmed |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_sort |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
author |
Aguiar, Bruno Guedes Alcoforado |
author_facet |
Aguiar, Bruno Guedes Alcoforado Coelho, Daniela Lemos Costa, Dorcas Lamounier Drumond, Betânia Paiva Coelho, Luiz Felipe Leomil Figueiredo, Lívio Carvalho Zacarias, Danielle Alves Silva, Jailthon Carlos Da Alonso, Diego Peres [UNESP] Ribolla, Paulo Eduardo Martins Ishikawa, Edna Aoba Yassui Gaído, Samara Belchior Costa, Carlos Henrique Nery |
author_role |
author |
author2 |
Coelho, Daniela Lemos Costa, Dorcas Lamounier Drumond, Betânia Paiva Coelho, Luiz Felipe Leomil Figueiredo, Lívio Carvalho Zacarias, Danielle Alves Silva, Jailthon Carlos Da Alonso, Diego Peres [UNESP] Ribolla, Paulo Eduardo Martins Ishikawa, Edna Aoba Yassui Gaído, Samara Belchior Costa, Carlos Henrique Nery |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Piauí (UFPI) Universidade Federal de Juiz de Fora (UFJF) Universidade Federal de Alfenas (UNIFAL) Universidade Federal da Paraíba (UFPB) Universidade Estadual Paulista (Unesp) Universidade Federal do Pará Núcleo de Medicina Tropical Laboratório de Biologia Molecular |
dc.contributor.author.fl_str_mv |
Aguiar, Bruno Guedes Alcoforado Coelho, Daniela Lemos Costa, Dorcas Lamounier Drumond, Betânia Paiva Coelho, Luiz Felipe Leomil Figueiredo, Lívio Carvalho Zacarias, Danielle Alves Silva, Jailthon Carlos Da Alonso, Diego Peres [UNESP] Ribolla, Paulo Eduardo Martins Ishikawa, Edna Aoba Yassui Gaído, Samara Belchior Costa, Carlos Henrique Nery |
dc.subject.por.fl_str_mv |
Genetic diversity Kala-azar Visceral leishmaniasis Macrophage inhibition factor Tropical diseases Single nucleotide polymorphism |
topic |
Genetic diversity Kala-azar Visceral leishmaniasis Macrophage inhibition factor Tropical diseases Single nucleotide polymorphism |
description |
Introduction Kala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar. Methods To determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification. Results The nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival. Conclusions NAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 2015-02-02T12:39:13Z 2015-02-02T12:39:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-0183-2014 Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 47, n. 5, p. 593-598, 2014. 0037-8682 http://hdl.handle.net/11449/114114 10.1590/0037-8682-0183-2014 S0037-86822014000500593 S0037-86822014000500593.pdf 3577149748456880 0000-0001-8735-6090 |
url |
http://dx.doi.org/10.1590/0037-8682-0183-2014 http://hdl.handle.net/11449/114114 |
identifier_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 47, n. 5, p. 593-598, 2014. 0037-8682 10.1590/0037-8682-0183-2014 S0037-86822014000500593 S0037-86822014000500593.pdf 3577149748456880 0000-0001-8735-6090 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical 1.358 0,658 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
593-598 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790031254913024 |