Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cbpb.2020.110467 http://hdl.handle.net/11449/197244 |
Resumo: | Gonadal sex differentiation in teleost fish shows greater plasticity as compared to other vertebrates, as it can be influenced by a variety of factors such as exogenous sex steroids. Exogenous estrogens, such as 17 beta-estradiol (E2), can induce feminization when administered during early embryonic development. However, the mechanisms underlying the E2-induced feminization are not fully understood, especially in Neotropical species. Therefore, the aim of this study was to evaluate the effects of E2 administration on the phenotypic sex characteristics, histological assessment of the gonads, and the expression of selected genes in Astyanax altiparanae exposed to dietary E2 prior to gonadal differentiation. M 4 days post-hatch (dph), groups of 30-40 undifferentiated larvae were fed with a diet containing varying amounts of E2 for 28 days, and fish were sampled at 90 dph. Previous studies revealed that ovary formation in A. altiparanae occurred at 58 dph, whereas the first sign of testis formation was found at 73 dph. In relation to the control, E2 exposure increased the proportion of phenotypic females in 120% and 148.4% for 4 and 6 mg E2/Kg, respectively. However, histological analysis revealed that treatments did not affect gonadal sex ratio between males and females, but induced intersex (testisova) in the group treated with 6 mg E2/Kg food. Treatment with E2 also altered gonadal transcript levels of a selected number of genes implicated in sexual differentiation. Males overexpressed dmrt1, sox9 and amh following E2 treatment as compared to control. Females showed increased mRNA levels of dmrtl and sox9, which might be related to the down-regulation of cyp19a1a after E2 exposure. In summary, E2 exposure during early gonadal development affected male secondary characteristics without changing the gonadal sex ratio, and altered expression of genes implicated in sexual differentiation. |
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Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanaeAstyanax altiparanaeSex differentiation17 beta-estradiolIntersexGonadal resilienceGonadal sex differentiation in teleost fish shows greater plasticity as compared to other vertebrates, as it can be influenced by a variety of factors such as exogenous sex steroids. Exogenous estrogens, such as 17 beta-estradiol (E2), can induce feminization when administered during early embryonic development. However, the mechanisms underlying the E2-induced feminization are not fully understood, especially in Neotropical species. Therefore, the aim of this study was to evaluate the effects of E2 administration on the phenotypic sex characteristics, histological assessment of the gonads, and the expression of selected genes in Astyanax altiparanae exposed to dietary E2 prior to gonadal differentiation. M 4 days post-hatch (dph), groups of 30-40 undifferentiated larvae were fed with a diet containing varying amounts of E2 for 28 days, and fish were sampled at 90 dph. Previous studies revealed that ovary formation in A. altiparanae occurred at 58 dph, whereas the first sign of testis formation was found at 73 dph. In relation to the control, E2 exposure increased the proportion of phenotypic females in 120% and 148.4% for 4 and 6 mg E2/Kg, respectively. However, histological analysis revealed that treatments did not affect gonadal sex ratio between males and females, but induced intersex (testisova) in the group treated with 6 mg E2/Kg food. Treatment with E2 also altered gonadal transcript levels of a selected number of genes implicated in sexual differentiation. Males overexpressed dmrt1, sox9 and amh following E2 treatment as compared to control. Females showed increased mRNA levels of dmrtl and sox9, which might be related to the down-regulation of cyp19a1a after E2 exposure. In summary, E2 exposure during early gonadal development affected male secondary characteristics without changing the gonadal sex ratio, and altered expression of genes implicated in sexual differentiation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, Reprod & Mol Biol Grp, Botucatu, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, BrazilSao Paulo State Univ, Aquaculture Ctr CAUNESP, Jaboticabal, SP, BrazilWest Virginia Univ, Dept Orthopaed Musculoskeletal Res, Morgantown, WV 26506 USAUniv Fed Para, UFPA, Fac Med, Lab Morfofisiol, Altamira, Para, BrazilSao Paulo State Univ, Inst Biosci, Dept Struct & Funct Biol, Reprod & Mol Biol Grp, Botucatu, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, BrazilSao Paulo State Univ, Aquaculture Ctr CAUNESP, Jaboticabal, SP, BrazilCNPq: 190111/2014-3FAPESP: 14/07620-7FAPESP: 14/25313-4FAPESP: 16/12101-4FAPESP: 18/10265-5Elsevier B.V.Universidade Estadual Paulista (Unesp)West Virginia UnivUniversidade Federal do Pará (UFPA)Martinez-Bengochea, A. [UNESP]Doretto, L. [UNESP]Rosa, I. F. [UNESP]Oliveira, M. A. [UNESP]Silva, C. [UNESP]Silva, D. M. Z. A. [UNESP]Santos, G. R. [UNESP]Santos, J. S. F. [UNESP]Avelar, M. M. [UNESP]Silva, L. [UNESP]Lucianelli-Junior, D.Souza, E. R. B. [UNESP]Silva, R. C. [UNESP]Stewart, A. B.Nakaghi, L. S. O. [UNESP]Valentin, F. N.Nobrega, R. H. [UNESP]2020-12-10T20:10:40Z2020-12-10T20:10:40Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1016/j.cbpb.2020.110467Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology. New York: Elsevier Science Inc, v. 248, 10 p., 2020.1096-4959http://hdl.handle.net/11449/19724410.1016/j.cbpb.2020.110467WOS:000563860500004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Biochemistry And Physiology B-biochemistry & Molecular Biologyinfo:eu-repo/semantics/openAccess2021-10-23T12:31:14Zoai:repositorio.unesp.br:11449/197244Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:31:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae |
title |
Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae |
spellingShingle |
Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae Martinez-Bengochea, A. [UNESP] Astyanax altiparanae Sex differentiation 17 beta-estradiol Intersex Gonadal resilience |
title_short |
Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae |
title_full |
Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae |
title_fullStr |
Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae |
title_full_unstemmed |
Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae |
title_sort |
Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae |
author |
Martinez-Bengochea, A. [UNESP] |
author_facet |
Martinez-Bengochea, A. [UNESP] Doretto, L. [UNESP] Rosa, I. F. [UNESP] Oliveira, M. A. [UNESP] Silva, C. [UNESP] Silva, D. M. Z. A. [UNESP] Santos, G. R. [UNESP] Santos, J. S. F. [UNESP] Avelar, M. M. [UNESP] Silva, L. [UNESP] Lucianelli-Junior, D. Souza, E. R. B. [UNESP] Silva, R. C. [UNESP] Stewart, A. B. Nakaghi, L. S. O. [UNESP] Valentin, F. N. Nobrega, R. H. [UNESP] |
author_role |
author |
author2 |
Doretto, L. [UNESP] Rosa, I. F. [UNESP] Oliveira, M. A. [UNESP] Silva, C. [UNESP] Silva, D. M. Z. A. [UNESP] Santos, G. R. [UNESP] Santos, J. S. F. [UNESP] Avelar, M. M. [UNESP] Silva, L. [UNESP] Lucianelli-Junior, D. Souza, E. R. B. [UNESP] Silva, R. C. [UNESP] Stewart, A. B. Nakaghi, L. S. O. [UNESP] Valentin, F. N. Nobrega, R. H. [UNESP] |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) West Virginia Univ Universidade Federal do Pará (UFPA) |
dc.contributor.author.fl_str_mv |
Martinez-Bengochea, A. [UNESP] Doretto, L. [UNESP] Rosa, I. F. [UNESP] Oliveira, M. A. [UNESP] Silva, C. [UNESP] Silva, D. M. Z. A. [UNESP] Santos, G. R. [UNESP] Santos, J. S. F. [UNESP] Avelar, M. M. [UNESP] Silva, L. [UNESP] Lucianelli-Junior, D. Souza, E. R. B. [UNESP] Silva, R. C. [UNESP] Stewart, A. B. Nakaghi, L. S. O. [UNESP] Valentin, F. N. Nobrega, R. H. [UNESP] |
dc.subject.por.fl_str_mv |
Astyanax altiparanae Sex differentiation 17 beta-estradiol Intersex Gonadal resilience |
topic |
Astyanax altiparanae Sex differentiation 17 beta-estradiol Intersex Gonadal resilience |
description |
Gonadal sex differentiation in teleost fish shows greater plasticity as compared to other vertebrates, as it can be influenced by a variety of factors such as exogenous sex steroids. Exogenous estrogens, such as 17 beta-estradiol (E2), can induce feminization when administered during early embryonic development. However, the mechanisms underlying the E2-induced feminization are not fully understood, especially in Neotropical species. Therefore, the aim of this study was to evaluate the effects of E2 administration on the phenotypic sex characteristics, histological assessment of the gonads, and the expression of selected genes in Astyanax altiparanae exposed to dietary E2 prior to gonadal differentiation. M 4 days post-hatch (dph), groups of 30-40 undifferentiated larvae were fed with a diet containing varying amounts of E2 for 28 days, and fish were sampled at 90 dph. Previous studies revealed that ovary formation in A. altiparanae occurred at 58 dph, whereas the first sign of testis formation was found at 73 dph. In relation to the control, E2 exposure increased the proportion of phenotypic females in 120% and 148.4% for 4 and 6 mg E2/Kg, respectively. However, histological analysis revealed that treatments did not affect gonadal sex ratio between males and females, but induced intersex (testisova) in the group treated with 6 mg E2/Kg food. Treatment with E2 also altered gonadal transcript levels of a selected number of genes implicated in sexual differentiation. Males overexpressed dmrt1, sox9 and amh following E2 treatment as compared to control. Females showed increased mRNA levels of dmrtl and sox9, which might be related to the down-regulation of cyp19a1a after E2 exposure. In summary, E2 exposure during early gonadal development affected male secondary characteristics without changing the gonadal sex ratio, and altered expression of genes implicated in sexual differentiation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:10:40Z 2020-12-10T20:10:40Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cbpb.2020.110467 Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology. New York: Elsevier Science Inc, v. 248, 10 p., 2020. 1096-4959 http://hdl.handle.net/11449/197244 10.1016/j.cbpb.2020.110467 WOS:000563860500004 |
url |
http://dx.doi.org/10.1016/j.cbpb.2020.110467 http://hdl.handle.net/11449/197244 |
identifier_str_mv |
Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology. New York: Elsevier Science Inc, v. 248, 10 p., 2020. 1096-4959 10.1016/j.cbpb.2020.110467 WOS:000563860500004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964847551545344 |