Creatine supplementation and oxidative stress in rat liver
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1550-2783-10-54 http://hdl.handle.net/11449/112800 |
Resumo: | Background: The objective of this study was to determine the effects of creatine supplementation on liver biomarkers of oxidative stress in exercise-trained rats.Methods: Forty 90-day-old adult male Wistar rats were assigned to four groups for the eight-week experiment. Control group (C) rats received a balanced control diet; creatine control group (CCr) rats received a balanced diet supplemented with 2% creatine; trained group (T) rats received a balanced diet and intense exercise training equivalent to the maximal lactate steady state phase; and supplemented-trained (TCr) rats were given a balanced diet supplemented with 2% creatine and subjected to intense exercise training equivalent to the maximal lactate steady state phase. At the end of the experimental period, concentrations of creatine, hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS) were measured as well as the enzyme activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-GPx) and catalase (CAT). Liver tissue levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio were also determined.Results: Hepatic creatine levels were highest in the CCr and TCr groups with increased concentration of H2O2 observed in the T and TCr animal groups. SOD activity was decreased in the TCr group. GSH-GPx activity was increased in the T and TCr groups while CAT was elevated in the CCr and TCr groups. GSH, GGS and the GSH/GSSG ratio did not differ between all animal subsets.Conclusions: Our results demonstrate that creatine supplementation acts in an additive manner to physical training to raise antioxidant enzymes in rat liver. However, because markers of liver oxidative stress were unchanged, this finding may also indicate that training-induced oxidative stress cannot be ameliorated by creatine supplementation. |
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Creatine supplementation and oxidative stress in rat liverCreatine supplementationOxidative stressEnzymesTreadmill exerciseBackground: The objective of this study was to determine the effects of creatine supplementation on liver biomarkers of oxidative stress in exercise-trained rats.Methods: Forty 90-day-old adult male Wistar rats were assigned to four groups for the eight-week experiment. Control group (C) rats received a balanced control diet; creatine control group (CCr) rats received a balanced diet supplemented with 2% creatine; trained group (T) rats received a balanced diet and intense exercise training equivalent to the maximal lactate steady state phase; and supplemented-trained (TCr) rats were given a balanced diet supplemented with 2% creatine and subjected to intense exercise training equivalent to the maximal lactate steady state phase. At the end of the experimental period, concentrations of creatine, hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS) were measured as well as the enzyme activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-GPx) and catalase (CAT). Liver tissue levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio were also determined.Results: Hepatic creatine levels were highest in the CCr and TCr groups with increased concentration of H2O2 observed in the T and TCr animal groups. SOD activity was decreased in the TCr group. GSH-GPx activity was increased in the T and TCr groups while CAT was elevated in the CCr and TCr groups. GSH, GGS and the GSH/GSSG ratio did not differ between all animal subsets.Conclusions: Our results demonstrate that creatine supplementation acts in an additive manner to physical training to raise antioxidant enzymes in rat liver. However, because markers of liver oxidative stress were unchanged, this finding may also indicate that training-induced oxidative stress cannot be ameliorated by creatine supplementation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Phys Educ, Lab Nutr Metab & Exercise, Rio Claro, SP, BrazilUniv Fed Mato Grosso, UFMT, Fac Nutr, Postgrad Program Graduat Biosci, Culaba, Mato Grosso, BrazilUniv Estadual Paulista, Dept Phys Educ, Lab Nutr Metab & Exercise, Rio Claro, SP, BrazilFAPESP: 09/52063-0Biomed Central Ltd.Universidade Estadual Paulista (Unesp)Univ Fed Mato GrossoAraujo, Michel B. [UNESP]Moura, Leandro P. [UNESP]Vieira Junior, Roberto C.Junior, Marcelo C. [UNESP]Dalia, Rodrigo A. [UNESP]Sponton, Amanda C. [UNESP]Ribeiro, Carla [UNESP]Mello, Maria Alice R. [UNESP]2014-12-03T13:11:04Z2014-12-03T13:11:04Z2013-12-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1186/1550-2783-10-54Journal Of The International Society Of Sports Nutrition. London: Biomed Central Ltd, v. 10, 8 p., 2013.1550-2783http://hdl.handle.net/11449/11280010.1186/1550-2783-10-54WOS:000328961100001WOS000328961100001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the International Society of Sports Nutrition3.1350,775info:eu-repo/semantics/openAccess2023-12-31T06:14:36Zoai:repositorio.unesp.br:11449/112800Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-31T06:14:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Creatine supplementation and oxidative stress in rat liver |
title |
Creatine supplementation and oxidative stress in rat liver |
spellingShingle |
Creatine supplementation and oxidative stress in rat liver Araujo, Michel B. [UNESP] Creatine supplementation Oxidative stress Enzymes Treadmill exercise |
title_short |
Creatine supplementation and oxidative stress in rat liver |
title_full |
Creatine supplementation and oxidative stress in rat liver |
title_fullStr |
Creatine supplementation and oxidative stress in rat liver |
title_full_unstemmed |
Creatine supplementation and oxidative stress in rat liver |
title_sort |
Creatine supplementation and oxidative stress in rat liver |
author |
Araujo, Michel B. [UNESP] |
author_facet |
Araujo, Michel B. [UNESP] Moura, Leandro P. [UNESP] Vieira Junior, Roberto C. Junior, Marcelo C. [UNESP] Dalia, Rodrigo A. [UNESP] Sponton, Amanda C. [UNESP] Ribeiro, Carla [UNESP] Mello, Maria Alice R. [UNESP] |
author_role |
author |
author2 |
Moura, Leandro P. [UNESP] Vieira Junior, Roberto C. Junior, Marcelo C. [UNESP] Dalia, Rodrigo A. [UNESP] Sponton, Amanda C. [UNESP] Ribeiro, Carla [UNESP] Mello, Maria Alice R. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Fed Mato Grosso |
dc.contributor.author.fl_str_mv |
Araujo, Michel B. [UNESP] Moura, Leandro P. [UNESP] Vieira Junior, Roberto C. Junior, Marcelo C. [UNESP] Dalia, Rodrigo A. [UNESP] Sponton, Amanda C. [UNESP] Ribeiro, Carla [UNESP] Mello, Maria Alice R. [UNESP] |
dc.subject.por.fl_str_mv |
Creatine supplementation Oxidative stress Enzymes Treadmill exercise |
topic |
Creatine supplementation Oxidative stress Enzymes Treadmill exercise |
description |
Background: The objective of this study was to determine the effects of creatine supplementation on liver biomarkers of oxidative stress in exercise-trained rats.Methods: Forty 90-day-old adult male Wistar rats were assigned to four groups for the eight-week experiment. Control group (C) rats received a balanced control diet; creatine control group (CCr) rats received a balanced diet supplemented with 2% creatine; trained group (T) rats received a balanced diet and intense exercise training equivalent to the maximal lactate steady state phase; and supplemented-trained (TCr) rats were given a balanced diet supplemented with 2% creatine and subjected to intense exercise training equivalent to the maximal lactate steady state phase. At the end of the experimental period, concentrations of creatine, hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS) were measured as well as the enzyme activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-GPx) and catalase (CAT). Liver tissue levels of reduced glutathione (GSH), oxidized glutathione (GSSG) and the GSH/GSSG ratio were also determined.Results: Hepatic creatine levels were highest in the CCr and TCr groups with increased concentration of H2O2 observed in the T and TCr animal groups. SOD activity was decreased in the TCr group. GSH-GPx activity was increased in the T and TCr groups while CAT was elevated in the CCr and TCr groups. GSH, GGS and the GSH/GSSG ratio did not differ between all animal subsets.Conclusions: Our results demonstrate that creatine supplementation acts in an additive manner to physical training to raise antioxidant enzymes in rat liver. However, because markers of liver oxidative stress were unchanged, this finding may also indicate that training-induced oxidative stress cannot be ameliorated by creatine supplementation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-10 2014-12-03T13:11:04Z 2014-12-03T13:11:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1550-2783-10-54 Journal Of The International Society Of Sports Nutrition. London: Biomed Central Ltd, v. 10, 8 p., 2013. 1550-2783 http://hdl.handle.net/11449/112800 10.1186/1550-2783-10-54 WOS:000328961100001 WOS000328961100001.pdf |
url |
http://dx.doi.org/10.1186/1550-2783-10-54 http://hdl.handle.net/11449/112800 |
identifier_str_mv |
Journal Of The International Society Of Sports Nutrition. London: Biomed Central Ltd, v. 10, 8 p., 2013. 1550-2783 10.1186/1550-2783-10-54 WOS:000328961100001 WOS000328961100001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the International Society of Sports Nutrition 3.135 0,775 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965461418344448 |